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The map label for this gene is mnmC [H]

Identifier: 146302496

GI number: 146302496

Start: 5764560

End: 5765225

Strand: Reverse

Name: mnmC [H]

Synonym: Fjoh_4769

Alternate gene names: 146302496

Gene position: 5765225-5764560 (Counterclockwise)

Preceding gene: 146302498

Following gene: 146302495

Centisome position: 94.56

GC content: 33.78

Gene sequence:

>666_bases
GTGAAAAGGGAAATAATTAAAACGCTAGATGGTTCAACGACAATTCATTTGGAAGAGTGGAATGAAAGTTATCACTCTAA
ACATGGTGCAATACAGGAAGCAAAACACGTATTTATAATGAATGGACTTTCTTTATTTGAGAATAATCCGGTAACTATAA
TGGAAATTGGTTTTGGGACTGGTCTAAATGCTTTTATTACTTTTTTGGAGTCTGAAAATAAAGATCAAAAAATTGACTAT
GTTGGAGTAGAGGGATATCCGGTAAATGCAGAAGAAGTTTTAGCAATGAATTATGTCGAAGAACTCGAAGCATTGGAATT
TCGTAACATTTTTGAAGAAATGCATAAATCTGAATGGGGTAAAAAAATAGAAATTAGCAGCCGTTTCTCGTTAACCAAAA
GGAAACAATTTTTTGATGAAATAAACGATTTAGAAATTTTTGATTTGATTTACTTTGACGCCTTTGGATATCGGGTTCAG
CCGGAGCTTTGGAGTACTGAAATTTTTCAAAAAATGTACAATAGTTTAAAGCCAAATGGAGTTCTCGTTACTTATGCAGC
TCGTGGAGTTGTTAAAAGAAGTATGATTTCTGTTGGATTTACGGTCGAAAAATTGGCAGGGCCTCCGGGAAAACGAGAAA
TGTTTAGAGCCTTTAAAAAGGTTTAA

Upstream 100 bases:

>100_bases
AAACTGTCGTTTTTTAAGGCTTTACGCATTTGTTTTAATTAATTTTGACTGATAAAATAAACTCAAAATCAATAAAACAC
CATTGTAAAATTCGAAAATT

Downstream 100 bases:

>100_bases
GTGAGTTATTTAGAATAGTTCTATATTGATTTAAACGCATTAAGAAAACGTATTCGCTGCTAAAATTTTTAAAAAAATAA
GTTAAAAATAGTATCTGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKREIIKTLDGSTTIHLEEWNESYHSKHGAIQEAKHVFIMNGLSLFENNPVTIMEIGFGTGLNAFITFLESENKDQKIDY
VGVEGYPVNAEEVLAMNYVEELEALEFRNIFEEMHKSEWGKKIEISSRFSLTKRKQFFDEINDLEIFDLIYFDAFGYRVQ
PELWSTEIFQKMYNSLKPNGVLVTYAARGVVKRSMISVGFTVEKLAGPPGKREMFRAFKKV

Sequences:

>Translated_221_residues
MKREIIKTLDGSTTIHLEEWNESYHSKHGAIQEAKHVFIMNGLSLFENNPVTIMEIGFGTGLNAFITFLESENKDQKIDY
VGVEGYPVNAEEVLAMNYVEELEALEFRNIFEEMHKSEWGKKIEISSRFSLTKRKQFFDEINDLEIFDLIYFDAFGYRVQ
PELWSTEIFQKMYNSLKPNGVLVTYAARGVVKRSMISVGFTVEKLAGPPGKREMFRAFKKV
>Mature_221_residues
MKREIIKTLDGSTTIHLEEWNESYHSKHGAIQEAKHVFIMNGLSLFENNPVTIMEIGFGTGLNAFITFLESENKDQKIDY
VGVEGYPVNAEEVLAMNYVEELEALEFRNIFEEMHKSEWGKKIEISSRFSLTKRKQFFDEINDLEIFDLIYFDAFGYRVQ
PELWSTEIFQKMYNSLKPNGVLVTYAARGVVKRSMISVGFTVEKLAGPPGKREMFRAFKKV

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=224, Percent_Identity=26.7857142857143, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 25590; Mature: 25590

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREIIKTLDGSTTIHLEEWNESYHSKHGAIQEAKHVFIMNGLSLFENNPVTIMEIGFGT
CCCHHHHHCCCCEEEEEEHHHHHHHHHCCCHHHHCEEEEEECHHHHCCCCEEEEEECCCC
GLNAFITFLESENKDQKIDYVGVEGYPVNAEEVLAMNYVEELEALEFRNIFEEMHKSEWG
CHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKIEISSRFSLTKRKQFFDEINDLEIFDLIYFDAFGYRVQPELWSTEIFQKMYNSLKPNG
CEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCC
VLVTYAARGVVKRSMISVGFTVEKLAGPPGKREMFRAFKKV
EEEEEHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKREIIKTLDGSTTIHLEEWNESYHSKHGAIQEAKHVFIMNGLSLFENNPVTIMEIGFGT
CCCHHHHHCCCCEEEEEEHHHHHHHHHCCCHHHHCEEEEEECHHHHCCCCEEEEEECCCC
GLNAFITFLESENKDQKIDYVGVEGYPVNAEEVLAMNYVEELEALEFRNIFEEMHKSEWG
CHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKIEISSRFSLTKRKQFFDEINDLEIFDLIYFDAFGYRVQPELWSTEIFQKMYNSLKPNG
CEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCCCCCC
VLVTYAARGVVKRSMISVGFTVEKLAGPPGKREMFRAFKKV
EEEEEHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA