Click here to switch to the map view.

The map label for this gene is pchA [H]

Identifier: 146302445

GI number: 146302445

Start: 5697412

End: 5698482

Strand: Reverse

Name: pchA [H]

Synonym: Fjoh_4718

Alternate gene names: 146302445

Gene position: 5698482-5697412 (Counterclockwise)

Preceding gene: 146302446

Following gene: 146302444

Centisome position: 93.47

GC content: 32.59

Gene sequence:

>1071_bases
ATGAATCCATTTTTCTTAAAATTTAAAAATCATAAAGAACAAAATTTACCTTTTGTACTTTATTCAAAACCCAATTCTGG
AAATCTTATTGGTTTTTTACAGCAAAATGATACTTTACATCAAGTTGCAGATTATACAGAGAAAGGATTTGTTTTTGCTT
CTTTTGATGAAAAACAGCTTGTTTTAATTCCGGAGAACGAATCGGAAATTATTACTTCACAAAAAGAAATCACTGCATTT
GAATCTTTTGAAACAGATGATTTAAATTTAGATGCTGCAGCTAAAACGCAATACGAAAGTTTAGTTGCAAAAGGCGTTGA
GGCTATTAAAAATGAAGAGTTTAAAAAAGTAGTTTTATCAAGAAGCGAAAAAGTCAATTTAGCTGAATTTGATTTTATTG
AAACCTTTCAGCATTTGATTCAAATTTATCCTGCTACTTTCTGTTATTGCTTTTTTCATCCAAAAGTTGGATTTTGGATG
GGAGCAACGCCTGAAAAACTTTTAAAAGCAAACGGAAATGTTTTTGAAACCATGGCTTTGGCCGGAACACAAAAAGATAA
TTCTGAAACTGAAATAGTCTGGCAGCAAAAAGAAAAAGACGAACAGCAGTTTGTAACTGATTTTATTGTAAAAAGACTGA
GAGAATTTACGGCTTCGGTTGTAGTTTCTGAACCGTACAGTTTAAAAGCAGGTTCTATCTGGCATATTAAAACGGATATT
TCGGGAGTTTTAAAGGAGAATTCAACTTTAGAAGAAGTTATTGATACGCTTCATCCAACTCCTGCTGTGTGCGGACTTCC
AAAGAAAAAAGCAAAAGCATTTATTATCGAAAACGAGAATTACGATAGAACTTTTTATACCGGATTTCTGGGTGAATTAA
ACAGCAGTTTTACTGGAAATACTGTTAGTTCTGATTTATTCGTAAATTTACGAAGCATGCAGATTCTGGATAAAACAGCC
ATTTTATACATGGGATGCGGCATAACTAAAGAAAGTATTCCCGAAAAAGAATGGGAGGAAAGCGTCAATAAATCGATGAC
GATGAAAAGAGTATTGAGAGTTATGAGTTAG

Upstream 100 bases:

>100_bases
TCATCTTTGGGAAATCAAAATTACAGATGAAGAAGGAAATCTGGTTTCGCTTTGTAAACTGACGAATATGGTTTTAGACA
GAAAGAAAAGTGAATAAATT

Downstream 100 bases:

>100_bases
AAGTTATGAATTATGAGTTTACAGTCGCAGTCGCAGTTTGCAGATACTGAGACTAAAAACTGAGACTGAAAACTGAGACT
GCGACTCAACCTGAAACAAA

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNPFFLKFKNHKEQNLPFVLYSKPNSGNLIGFLQQNDTLHQVADYTEKGFVFASFDEKQLVLIPENESEIITSQKEITAF
ESFETDDLNLDAAAKTQYESLVAKGVEAIKNEEFKKVVLSRSEKVNLAEFDFIETFQHLIQIYPATFCYCFFHPKVGFWM
GATPEKLLKANGNVFETMALAGTQKDNSETEIVWQQKEKDEQQFVTDFIVKRLREFTASVVVSEPYSLKAGSIWHIKTDI
SGVLKENSTLEEVIDTLHPTPAVCGLPKKKAKAFIIENENYDRTFYTGFLGELNSSFTGNTVSSDLFVNLRSMQILDKTA
ILYMGCGITKESIPEKEWEESVNKSMTMKRVLRVMS

Sequences:

>Translated_356_residues
MNPFFLKFKNHKEQNLPFVLYSKPNSGNLIGFLQQNDTLHQVADYTEKGFVFASFDEKQLVLIPENESEIITSQKEITAF
ESFETDDLNLDAAAKTQYESLVAKGVEAIKNEEFKKVVLSRSEKVNLAEFDFIETFQHLIQIYPATFCYCFFHPKVGFWM
GATPEKLLKANGNVFETMALAGTQKDNSETEIVWQQKEKDEQQFVTDFIVKRLREFTASVVVSEPYSLKAGSIWHIKTDI
SGVLKENSTLEEVIDTLHPTPAVCGLPKKKAKAFIIENENYDRTFYTGFLGELNSSFTGNTVSSDLFVNLRSMQILDKTA
ILYMGCGITKESIPEKEWEESVNKSMTMKRVLRVMS
>Mature_356_residues
MNPFFLKFKNHKEQNLPFVLYSKPNSGNLIGFLQQNDTLHQVADYTEKGFVFASFDEKQLVLIPENESEIITSQKEITAF
ESFETDDLNLDAAAKTQYESLVAKGVEAIKNEEFKKVVLSRSEKVNLAEFDFIETFQHLIQIYPATFCYCFFHPKVGFWM
GATPEKLLKANGNVFETMALAGTQKDNSETEIVWQQKEKDEQQFVTDFIVKRLREFTASVVVSEPYSLKAGSIWHIKTDI
SGVLKENSTLEEVIDTLHPTPAVCGLPKKKAKAFIIENENYDRTFYTGFLGELNSSFTGNTVSSDLFVNLRSMQILDKTA
ILYMGCGITKESIPEKEWEESVNKSMTMKRVLRVMS

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=313, Percent_Identity=27.1565495207668, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082077, Length=273, Percent_Identity=28.2051282051282, Blast_Score=102, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320935, Length=288, Percent_Identity=27.0833333333333, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 40589; Mature: 40589

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPFFLKFKNHKEQNLPFVLYSKPNSGNLIGFLQQNDTLHQVADYTEKGFVFASFDEKQL
CCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCEE
VLIPENESEIITSQKEITAFESFETDDLNLDAAAKTQYESLVAKGVEAIKNEEFKKVVLS
EEEECCCCHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
RSEKVNLAEFDFIETFQHLIQIYPATFCYCFFHPKVGFWMGATPEKLLKANGNVFETMAL
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEECCCCHHHHHHCCCCEEEEHHH
AGTQKDNSETEIVWQQKEKDEQQFVTDFIVKRLREFTASVVVSEPYSLKAGSIWHIKTDI
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCH
SGVLKENSTLEEVIDTLHPTPAVCGLPKKKAKAFIIENENYDRTFYTGFLGELNSSFTGN
HHHHHCCCCHHHHHHHHCCCCCCCCCCCHHCCEEEEECCCCCCCEEHHHHHHCCCCCCCC
TVSSDLFVNLRSMQILDKTAILYMGCGITKESIPEKEWEESVNKSMTMKRVLRVMS
CCCHHHEEEHHHHHHHHHHEEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNPFFLKFKNHKEQNLPFVLYSKPNSGNLIGFLQQNDTLHQVADYTEKGFVFASFDEKQL
CCCEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCEEEECCCCEE
VLIPENESEIITSQKEITAFESFETDDLNLDAAAKTQYESLVAKGVEAIKNEEFKKVVLS
EEEECCCCHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHC
RSEKVNLAEFDFIETFQHLIQIYPATFCYCFFHPKVGFWMGATPEKLLKANGNVFETMAL
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEECCCCHHHHHHCCCCEEEEHHH
AGTQKDNSETEIVWQQKEKDEQQFVTDFIVKRLREFTASVVVSEPYSLKAGSIWHIKTDI
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCH
SGVLKENSTLEEVIDTLHPTPAVCGLPKKKAKAFIIENENYDRTFYTGFLGELNSSFTGN
HHHHHCCCCHHHHHHHHCCCCCCCCCCCHHCCEEEEECCCCCCCEEHHHHHHCCCCCCCC
TVSSDLFVNLRSMQILDKTAILYMGCGITKESIPEKEWEESVNKSMTMKRVLRVMS
CCCHHHEEEHHHHHHHHHHEEEEEECCCCHHCCCHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]