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The map label for this gene is panB

Identifier: 146302438

GI number: 146302438

Start: 5687162

End: 5687980

Strand: Direct

Name: panB

Synonym: Fjoh_4711

Alternate gene names: 146302438

Gene position: 5687162-5687980 (Clockwise)

Preceding gene: 146302437

Following gene: 146302439

Centisome position: 93.28

GC content: 37.97

Gene sequence:

>819_bases
ATGTCAACAGCAAAAAAAGATTATAAAAGAATCACAACAAAGTCATTAATTGAAATGAAAAGCAATGGAGAAAAAATCTC
TATGCTTACGGCGTATGATTACACAATGGCTAAAATTGTTGATACCGCAGGAGTCGATGTAATTTTGGTGGGAGATTCTG
CTTCAAATGTTATGGCGGGCCATGAAACGACTCTTCCAATTACTTTAGATCAAATGATTTATCACGCTTCTTCTGTAGTT
CGCGCTGTAGAGAGAGGTTTGGTTGTGGTTGATCTTCCTTTTGGAAGTTACCAGTCAGATCCTAAAGAGGCTTTGCGTTC
TGCTATCAGAATCATGAAAGAAAGCGGCGCCCACGCTGTGAAATTAGAAGGCGGAAAAGAAATTAAAGAATCAATCAAAA
AAATATTAAACGCAGGAATTCCGGTTATGGGCCACTTAGGTTTAACGCCTCAGTCTATCTATAAATTTGGAACTTACAGC
GTTCGTGCAAAAGAAGAACAAGAAGCTGAAAAACTTATTGAAGATGCTCAAATGCTTGAAAAAGTAGGATGTTTTGGAGT
TGTTCTTGAAAAAATCCCAGCTGATTTAGCTAAAAAAGTAGCCGATAGTATTTCAATCCCAGTTATCGGAATTGGTGCCG
GCGGTGCGGTAGACGGACAAGTTTTAGTTATTCACGATATGTTAGGAATGAATAATGAATTCAGCCCGCGTTTCTTACGC
CGTTACTTAAACTTATACGAAGAAATGACTAAAGCAATTGGTCAATATGCTGCTGATGTAAAATCAAGCGATTTCCCTAA
TGCTAGTGAGCAATATTAA

Upstream 100 bases:

>100_bases
TTTTTAAAGACGGAAAGCTTTTTTCTGTCGAAATATTCTTTTATTGCTGATTCCCCTTTTACAATTATTCAATACTTTTA
CATTATCAAAAAAAATTAAA

Downstream 100 bases:

>100_bases
TTTTTAAAGATGCTAAGGTTCTAAGGTTCTGAGATTTTTTTTAGAGACAAAGATCCAAAGTGACAAAGGTTCAAAGGCTT
TTTAAATTGAACAAAACTTA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSTAKKDYKRITTKSLIEMKSNGEKISMLTAYDYTMAKIVDTAGVDVILVGDSASNVMAGHETTLPITLDQMIYHASSVV
RAVERGLVVVDLPFGSYQSDPKEALRSAIRIMKESGAHAVKLEGGKEIKESIKKILNAGIPVMGHLGLTPQSIYKFGTYS
VRAKEEQEAEKLIEDAQMLEKVGCFGVVLEKIPADLAKKVADSISIPVIGIGAGGAVDGQVLVIHDMLGMNNEFSPRFLR
RYLNLYEEMTKAIGQYAADVKSSDFPNASEQY

Sequences:

>Translated_272_residues
MSTAKKDYKRITTKSLIEMKSNGEKISMLTAYDYTMAKIVDTAGVDVILVGDSASNVMAGHETTLPITLDQMIYHASSVV
RAVERGLVVVDLPFGSYQSDPKEALRSAIRIMKESGAHAVKLEGGKEIKESIKKILNAGIPVMGHLGLTPQSIYKFGTYS
VRAKEEQEAEKLIEDAQMLEKVGCFGVVLEKIPADLAKKVADSISIPVIGIGAGGAVDGQVLVIHDMLGMNNEFSPRFLR
RYLNLYEEMTKAIGQYAADVKSSDFPNASEQY
>Mature_271_residues
STAKKDYKRITTKSLIEMKSNGEKISMLTAYDYTMAKIVDTAGVDVILVGDSASNVMAGHETTLPITLDQMIYHASSVVR
AVERGLVVVDLPFGSYQSDPKEALRSAIRIMKESGAHAVKLEGGKEIKESIKKILNAGIPVMGHLGLTPQSIYKFGTYSV
RAKEEQEAEKLIEDAQMLEKVGCFGVVLEKIPADLAKKVADSISIPVIGIGAGGAVDGQVLVIHDMLGMNNEFSPRFLRR
YLNLYEEMTKAIGQYAADVKSSDFPNASEQY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=262, Percent_Identity=38.9312977099237, Blast_Score=210, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6319653, Length=273, Percent_Identity=38.0952380952381, Blast_Score=205, Evalue=8e-54,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_FLAJ1 (A5FAQ5)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001197029.1
- ProteinModelPortal:   A5FAQ5
- SMR:   A5FAQ5
- STRING:   A5FAQ5
- GeneID:   5092409
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_4711
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- BioCyc:   FJOH376686:FJOH_4711-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29640; Mature: 29508

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: ACT_SITE 190-190 BINDING 91-91 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAKKDYKRITTKSLIEMKSNGEKISMLTAYDYTMAKIVDTAGVDVILVGDSASNVMAG
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCC
HETTLPITLDQMIYHASSVVRAVERGLVVVDLPFGSYQSDPKEALRSAIRIMKESGAHAV
CCCCCCEEHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEE
KLEGGKEIKESIKKILNAGIPVMGHLGLTPQSIYKFGTYSVRAKEEQEAEKLIEDAQMLE
EECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHH
KVGCFGVVLEKIPADLAKKVADSISIPVIGIGAGGAVDGQVLVIHDMLGMNNEFSPRFLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHCCCCCCCHHHHH
RYLNLYEEMTKAIGQYAADVKSSDFPNASEQY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STAKKDYKRITTKSLIEMKSNGEKISMLTAYDYTMAKIVDTAGVDVILVGDSASNVMAG
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHCCCEEEEECCCCCCCCCC
HETTLPITLDQMIYHASSVVRAVERGLVVVDLPFGSYQSDPKEALRSAIRIMKESGAHAV
CCCCCCEEHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEE
KLEGGKEIKESIKKILNAGIPVMGHLGLTPQSIYKFGTYSVRAKEEQEAEKLIEDAQMLE
EECCCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCEEECCHHHHHHHHHHHHHHHH
KVGCFGVVLEKIPADLAKKVADSISIPVIGIGAGGAVDGQVLVIHDMLGMNNEFSPRFLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHCCCCCCCHHHHH
RYLNLYEEMTKAIGQYAADVKSSDFPNASEQY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA