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The map label for this gene is rsmE [H]

Identifier: 146302321

GI number: 146302321

Start: 5557900

End: 5558607

Strand: Direct

Name: rsmE [H]

Synonym: Fjoh_4594

Alternate gene names: 146302321

Gene position: 5557900-5558607 (Clockwise)

Preceding gene: 146302320

Following gene: 146302322

Centisome position: 91.16

GC content: 33.62

Gene sequence:

>708_bases
ATGCAGTTATTTTACAATCCGGATATAGACGAAACAACCGAAAGTTTTTCTTTTGATAAAGAAGAAAGCAAACACATTAT
AAAAGTTTTACGCAAGAAAGATTCAGATATTCTTCACGTTACAAATGGTCAGGGATTATTGTTTGAAACCGAAATTACGC
TGGCATCTGATAACAAATGTATCGTGAGCGTTCTTTCAATAAAAAAATCTCCAGAACCAAAATTTCGTCTGCACCTTGCT
GTTGCGCCTACTAAAATGAATGACCGTTTTGAGTGGTTTTTAGAAAAAGCTACAGAAATAGGCATTCAGGAAATTACACC
AATTATCTGCGATCGTTCTGAAAGAAAAGTGATTAATTTAGAGCGTTTTGAAAAAATAGTTTTATCGGCAATGAAACAAT
CTAATGAAATGTTTCTTCCAAAATTAAACGATGCTGTTTCGTTTAAAGAATTTATCAAGCAAAAAAATCAAGGCTTGCAG
TTTATTGCGCACTGCGAAGAAACCGATAAAAAATCTTTGAAAGAATCTTTAAAATCAAACGAAAACGTAACCTTATTAAT
TGGTCCCGAAGGTGATTTTTCTGAAAAAGAAATTGCATTGGCTCTTGAAAACAACTATAAACCGGTAACATTAGGAAATA
CTCGTCTGCGTACAGAAACTGCCGCAGTTGTGGCCTGCCATAGTGTTGTATTTTTTAATGAAGTTTAA

Upstream 100 bases:

>100_bases
ATAATGCCGCAATGATAGGAATTGTAGGCTACCAAAAATATTTATCAAAACGTTTTGAAGATTCTTCAGTAGTTTCTAAA
GCACGAATTCAGTTTTAATT

Downstream 100 bases:

>100_bases
TAAATTCCGAGGGTTTTGTGAAAAACAAAACGAGTAGACAAATTACAATTCTATGAAAAAAATATTTTATTTGCTTTTAC
TTTTTTCAATCACTTCTTTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MQLFYNPDIDETTESFSFDKEESKHIIKVLRKKDSDILHVTNGQGLLFETEITLASDNKCIVSVLSIKKSPEPKFRLHLA
VAPTKMNDRFEWFLEKATEIGIQEITPIICDRSERKVINLERFEKIVLSAMKQSNEMFLPKLNDAVSFKEFIKQKNQGLQ
FIAHCEETDKKSLKESLKSNENVTLLIGPEGDFSEKEIALALENNYKPVTLGNTRLRTETAAVVACHSVVFFNEV

Sequences:

>Translated_235_residues
MQLFYNPDIDETTESFSFDKEESKHIIKVLRKKDSDILHVTNGQGLLFETEITLASDNKCIVSVLSIKKSPEPKFRLHLA
VAPTKMNDRFEWFLEKATEIGIQEITPIICDRSERKVINLERFEKIVLSAMKQSNEMFLPKLNDAVSFKEFIKQKNQGLQ
FIAHCEETDKKSLKESLKSNENVTLLIGPEGDFSEKEIALALENNYKPVTLGNTRLRTETAAVVACHSVVFFNEV
>Mature_235_residues
MQLFYNPDIDETTESFSFDKEESKHIIKVLRKKDSDILHVTNGQGLLFETEITLASDNKCIVSVLSIKKSPEPKFRLHLA
VAPTKMNDRFEWFLEKATEIGIQEITPIICDRSERKVINLERFEKIVLSAMKQSNEMFLPKLNDAVSFKEFIKQKNQGLQ
FIAHCEETDKKSLKESLKSNENVTLLIGPEGDFSEKEIALALENNYKPVTLGNTRLRTETAAVVACHSVVFFNEV

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=229, Percent_Identity=28.3842794759825, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26856; Mature: 26856

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLFYNPDIDETTESFSFDKEESKHIIKVLRKKDSDILHVTNGQGLLFETEITLASDNKC
CCEEECCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCE
IVSVLSIKKSPEPKFRLHLAVAPTKMNDRFEWFLEKATEIGIQEITPIICDRSERKVINL
EEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHCCHHHCCCCCCCEECH
ERFEKIVLSAMKQSNEMFLPKLNDAVSFKEFIKQKNQGLQFIAHCEETDKKSLKESLKSN
HHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCC
ENVTLLIGPEGDFSEKEIALALENNYKPVTLGNTRLRTETAAVVACHSVVFFNEV
CCEEEEECCCCCCCCCEEEEEEECCCCEEEECCCEECHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MQLFYNPDIDETTESFSFDKEESKHIIKVLRKKDSDILHVTNGQGLLFETEITLASDNKC
CCEEECCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEEEEECCCCE
IVSVLSIKKSPEPKFRLHLAVAPTKMNDRFEWFLEKATEIGIQEITPIICDRSERKVINL
EEEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCHHHCCHHHCCCCCCCEECH
ERFEKIVLSAMKQSNEMFLPKLNDAVSFKEFIKQKNQGLQFIAHCEETDKKSLKESLKSN
HHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCC
ENVTLLIGPEGDFSEKEIALALENNYKPVTLGNTRLRTETAAVVACHSVVFFNEV
CCEEEEECCCCCCCCCEEEEEEECCCCEEEECCCEECHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]