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The map label for this gene is pyrB

Identifier: 146302272

GI number: 146302272

Start: 5490869

End: 5491795

Strand: Reverse

Name: pyrB

Synonym: Fjoh_4545

Alternate gene names: 146302272

Gene position: 5491795-5490869 (Counterclockwise)

Preceding gene: 146302273

Following gene: 146302271

Centisome position: 90.08

GC content: 37.76

Gene sequence:

>927_bases
ATGAAAGAATTAAGCGTAGATCATTTATTAGGAATAAAATATATCAATGAGAATGATATTAACCTGATTTTTGAAACGGC
AGATCATTTTAAAGAAGTCATTAACCGACCAATTAAAAAAGTTCCTTCATTACGAGATATTACCATTGCCAATATTTTCT
TCGAAAACAGTACCAGAACCAAACTCTCTTTCGAATTAGCGCAGAAACGTTTATCTGCTGATGTTATCAGTTTTTCTGCA
GCTCAGTCATCGGTTAAAAAAGGAGAAACCCTGATTGATACTGTAAATAATATCCTTTCGATGAAAGTTGATATGGTTGT
AATGCGCCACTCCAATCCCGGAGCGGCTTATTTTTTATCTAAAAATGTCAAAGCCAGTATCGTAAACGCCGGAGACGGAG
CACACGAACACCCAACACAGGCTTTGTTAGACAGTTATTCTATTAGAGAAAAACTGGGCGATGTAGCCGGAAAAAAAGTA
GTTATTGTAGGCGATATTCTACATTCGAGAGTTGCTTTATCTAACATATATGCTTTGCAGATGCAGGGCGCAGAAGTAAA
AGTATGCGGACCAAAAACCTTGATTCCAAGATATATCGAATCTCTTGGGGTAACGGTTGAACCAAATTTACGCAAAGCTT
TAGAATGGTGCGACGTTGCGAATATGCTTCGTGTACAAAACGAGCGTATGGATGTAAATTTCTTCCCGTCAACACGCGAA
TATGCACAACAATACGGAGTAGATAAACCGCTTTTGGATTCTCTTAATAAAGAAATCGTAATCATGCACCCGGGACCAAT
CAACAGAGGAGTAGAGATTACTTCTGAAGTGGCTGATTCTGATCATTCTGTAATCTTAAATCAGGTTGAGAATGGTGTTG
CGATTAGAATGGCAGTTATTTATCTTTTGGCTTCTAAGATTCAATAG

Upstream 100 bases:

>100_bases
ATAGACCGAATGACCGAATAGAGTAGCTGCTCTTTAAAGAAGCATTGAATGAAAGATATTTTTCATTCAGAATTTGACAA
ATAATATTAAAGAAACAAAA

Downstream 100 bases:

>100_bases
TTTTTAGTTTACAGTCGCAGTCTCAGTTTACAGTTTTGAAAACAGCTGTCACCCTGAGCGAAGTCGAAGGGCTTTTACGT
GACGAGGGCTTCGACTTCGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKELSVDHLLGIKYINENDINLIFETADHFKEVINRPIKKVPSLRDITIANIFFENSTRTKLSFELAQKRLSADVISFSA
AQSSVKKGETLIDTVNNILSMKVDMVVMRHSNPGAAYFLSKNVKASIVNAGDGAHEHPTQALLDSYSIREKLGDVAGKKV
VIVGDILHSRVALSNIYALQMQGAEVKVCGPKTLIPRYIESLGVTVEPNLRKALEWCDVANMLRVQNERMDVNFFPSTRE
YAQQYGVDKPLLDSLNKEIVIMHPGPINRGVEITSEVADSDHSVILNQVENGVAIRMAVIYLLASKIQ

Sequences:

>Translated_308_residues
MKELSVDHLLGIKYINENDINLIFETADHFKEVINRPIKKVPSLRDITIANIFFENSTRTKLSFELAQKRLSADVISFSA
AQSSVKKGETLIDTVNNILSMKVDMVVMRHSNPGAAYFLSKNVKASIVNAGDGAHEHPTQALLDSYSIREKLGDVAGKKV
VIVGDILHSRVALSNIYALQMQGAEVKVCGPKTLIPRYIESLGVTVEPNLRKALEWCDVANMLRVQNERMDVNFFPSTRE
YAQQYGVDKPLLDSLNKEIVIMHPGPINRGVEITSEVADSDHSVILNQVENGVAIRMAVIYLLASKIQ
>Mature_308_residues
MKELSVDHLLGIKYINENDINLIFETADHFKEVINRPIKKVPSLRDITIANIFFENSTRTKLSFELAQKRLSADVISFSA
AQSSVKKGETLIDTVNNILSMKVDMVVMRHSNPGAAYFLSKNVKASIVNAGDGAHEHPTQALLDSYSIREKLGDVAGKKV
VIVGDILHSRVALSNIYALQMQGAEVKVCGPKTLIPRYIESLGVTVEPNLRKALEWCDVANMLRVQNERMDVNFFPSTRE
YAQQYGVDKPLLDSLNKEIVIMHPGPINRGVEITSEVADSDHSVILNQVENGVAIRMAVIYLLASKIQ

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=35.1612903225806, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=34.9206349206349, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI193204318, Length=332, Percent_Identity=31.9277108433735, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=34.7133757961783, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24642586, Length=308, Percent_Identity=32.7922077922078, Blast_Score=148, Evalue=4e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_FLAJ1 (A5FB79)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001196863.1
- ProteinModelPortal:   A5FB79
- STRING:   A5FB79
- GeneID:   5092618
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_4545
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   FJOH376686:FJOH_4545-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34228; Mature: 34228

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSVDHLLGIKYINENDINLIFETADHFKEVINRPIKKVPSLRDITIANIFFENSTRT
CCCCCCHHHEEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
KLSFELAQKRLSADVISFSAAQSSVKKGETLIDTVNNILSMKVDMVVMRHSNPGAAYFLS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEE
KNVKASIVNAGDGAHEHPTQALLDSYSIREKLGDVAGKKVVIVGDILHSRVALSNIYALQ
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEEEE
MQGAEVKVCGPKTLIPRYIESLGVTVEPNLRKALEWCDVANMLRVQNERMDVNFFPSTRE
ECCCEEEEECCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
YAQQYGVDKPLLDSLNKEIVIMHPGPINRGVEITSEVADSDHSVILNQVENGVAIRMAVI
HHHHHCCCCHHHHCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHH
YLLASKIQ
HHHHHHCC
>Mature Secondary Structure
MKELSVDHLLGIKYINENDINLIFETADHFKEVINRPIKKVPSLRDITIANIFFENSTRT
CCCCCCHHHEEEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCC
KLSFELAQKRLSADVISFSAAQSSVKKGETLIDTVNNILSMKVDMVVMRHSNPGAAYFLS
EEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEE
KNVKASIVNAGDGAHEHPTQALLDSYSIREKLGDVAGKKVVIVGDILHSRVALSNIYALQ
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEEEE
MQGAEVKVCGPKTLIPRYIESLGVTVEPNLRKALEWCDVANMLRVQNERMDVNFFPSTRE
ECCCEEEEECCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
YAQQYGVDKPLLDSLNKEIVIMHPGPINRGVEITSEVADSDHSVILNQVENGVAIRMAVI
HHHHHCCCCHHHHCCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCHHHHHHHH
YLLASKIQ
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA