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The map label for this gene is dctA [H]

Identifier: 146302250

GI number: 146302250

Start: 5464652

End: 5465899

Strand: Reverse

Name: dctA [H]

Synonym: Fjoh_4523

Alternate gene names: 146302250

Gene position: 5465899-5464652 (Counterclockwise)

Preceding gene: 146302252

Following gene: 146302245

Centisome position: 89.65

GC content: 34.46

Gene sequence:

>1248_bases
ATGAATATAAACACTCCAAACCCTTCATCAAAAGCCAGTAAAAGCATTTTTCATGCTATAATTACCAACCTTACTTTTTG
GGTTTTGATTGCTATTCTTGCTGGTGTTTTACTAGGACATTTTTCGCCGGAAAATGGTGTGAAAATGGAAATTGTTGGAA
AAAGGTTTGTTGATCTTATTAAGCTTTTTATTGGTCCTATTATTTTCCTTACCATCGTTTTGGGAATTTCAGGAATGGGA
AATTTAAAAAAAGTAGGCCGAATTGGTGTAAAAGCCCTGGGTTATTTTGAAGTAGTTTCGACAGTTGCTTTAGCAATTGG
TGTAGCTGTAGCTTATATATTTCAACCTGGAAAAATAGACAAATCAGGATTAAGTTTAGGAGATGCAAGCCAATATACAA
ATGGAAGCGCAAAAGATTTTTCGTGGCTTCAGTTTTTCTTTTCAAATTTTACACTTCAGGTTTTATTAGCAGCAATTGTC
TGCGGAATTGCTTTGAATTTTTACCAAAAAAGAGAACAGACTATTTTGGTCTTAGAGCGATTTTCAAAATTTGTTTTTAC
GGGTTTAAAATATGTTATGTATCTCGCGCCAATTGGTGCTTTTGGCGGAATGGCCTATACAATTGGCAAGTTTGGATTAG
CGACTTTAATTCCGCTTGGAAAATTAATGCTCTGCGTTTACCTTACAATGGCGCTTTTTGTATTTTTAATTTTGGGCAGT
ATTTTGAGATATTATAAAATAAATATTCTCTCGATTTTAAAATACATCAAAGAAGAACTTTTACTGGTTTTAGGAACTTC
ATCTTCTGAAGCTGCTTTGCCCAGTATTATGGTAAAACTAGAAAGAATGGGCTGTAGTAAATCAGTTGTTGGATTGGTAA
TTCCAACAGGATATTCATTTAATCTTGACGGAACTTCGATTTACTTATCAATGTCTGTAATATTCCTTGCACAATTATAT
GATGTGCATTTGAGTTTTTTCGAAATCCTGACTGTAATAGGTATTTTGATGATTACATCAAAAGGAGCCGCAGGAGTAAC
CGGAAGCGGCTTTATTGTTTTAGCGTCTACTTTAACAGCGTTGCATAAAATTCCTGTTGAAGGATTGGCTTTTTTACTGG
GAGTTGATAAATTTATGAGTGAAGCCAGAGCCATTACAAACTTAATAGGAAATACTGTGGCAACCATTATAATTTCTAAA
ACAGAACGCGATTTTACGGAGCTGAATCTTGATCCGGTTTTGGAGTAG

Upstream 100 bases:

>100_bases
GAATCTAGATAAATGATAAGGCCTTGATTTATCAGGGACAGAATCCGGCTTATAGGTCTGCATTTTTTATATATTTGTGA
AACTATCTTAACCCTTTTTC

Downstream 100 bases:

>100_bases
TTTTTTTTAGCTGCAAAGGTTCAAAGTTGCAAAGGTGCAAAGGTTCAAAGATATAAAAGAACAAAGCCATTAAAACTCTG
CCCCTTTGTACCTTTGCACC

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNINTPNPSSKASKSIFHAIITNLTFWVLIAILAGVLLGHFSPENGVKMEIVGKRFVDLIKLFIGPIIFLTIVLGISGMG
NLKKVGRIGVKALGYFEVVSTVALAIGVAVAYIFQPGKIDKSGLSLGDASQYTNGSAKDFSWLQFFFSNFTLQVLLAAIV
CGIALNFYQKREQTILVLERFSKFVFTGLKYVMYLAPIGAFGGMAYTIGKFGLATLIPLGKLMLCVYLTMALFVFLILGS
ILRYYKINILSILKYIKEELLLVLGTSSSEAALPSIMVKLERMGCSKSVVGLVIPTGYSFNLDGTSIYLSMSVIFLAQLY
DVHLSFFEILTVIGILMITSKGAAGVTGSGFIVLASTLTALHKIPVEGLAFLLGVDKFMSEARAITNLIGNTVATIIISK
TERDFTELNLDPVLE

Sequences:

>Translated_415_residues
MNINTPNPSSKASKSIFHAIITNLTFWVLIAILAGVLLGHFSPENGVKMEIVGKRFVDLIKLFIGPIIFLTIVLGISGMG
NLKKVGRIGVKALGYFEVVSTVALAIGVAVAYIFQPGKIDKSGLSLGDASQYTNGSAKDFSWLQFFFSNFTLQVLLAAIV
CGIALNFYQKREQTILVLERFSKFVFTGLKYVMYLAPIGAFGGMAYTIGKFGLATLIPLGKLMLCVYLTMALFVFLILGS
ILRYYKINILSILKYIKEELLLVLGTSSSEAALPSIMVKLERMGCSKSVVGLVIPTGYSFNLDGTSIYLSMSVIFLAQLY
DVHLSFFEILTVIGILMITSKGAAGVTGSGFIVLASTLTALHKIPVEGLAFLLGVDKFMSEARAITNLIGNTVATIIISK
TERDFTELNLDPVLE
>Mature_415_residues
MNINTPNPSSKASKSIFHAIITNLTFWVLIAILAGVLLGHFSPENGVKMEIVGKRFVDLIKLFIGPIIFLTIVLGISGMG
NLKKVGRIGVKALGYFEVVSTVALAIGVAVAYIFQPGKIDKSGLSLGDASQYTNGSAKDFSWLQFFFSNFTLQVLLAAIV
CGIALNFYQKREQTILVLERFSKFVFTGLKYVMYLAPIGAFGGMAYTIGKFGLATLIPLGKLMLCVYLTMALFVFLILGS
ILRYYKINILSILKYIKEELLLVLGTSSSEAALPSIMVKLERMGCSKSVVGLVIPTGYSFNLDGTSIYLSMSVIFLAQLY
DVHLSFFEILTVIGILMITSKGAAGVTGSGFIVLASTLTALHKIPVEGLAFLLGVDKFMSEARAITNLIGNTVATIIISK
TERDFTELNLDPVLE

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI5032093, Length=396, Percent_Identity=26.5151515151515, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI223468564, Length=275, Percent_Identity=29.0909090909091, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI223468566, Length=263, Percent_Identity=29.277566539924, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4827012, Length=233, Percent_Identity=27.4678111587983, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI169790839, Length=223, Percent_Identity=27.3542600896861, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194239697, Length=164, Percent_Identity=29.8780487804878, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI66773030, Length=155, Percent_Identity=29.6774193548387, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40254478, Length=163, Percent_Identity=32.5153374233129, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21314632, Length=431, Percent_Identity=22.2737819025522, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI301601644, Length=152, Percent_Identity=28.2894736842105, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789947, Length=409, Percent_Identity=50.8557457212714, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790514, Length=412, Percent_Identity=36.4077669902913, Blast_Score=254, Evalue=7e-69,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=23.1920199501247, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=404, Percent_Identity=27.4752475247525, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI193206654, Length=455, Percent_Identity=23.956043956044, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71983106, Length=145, Percent_Identity=35.1724137931034, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71983099, Length=145, Percent_Identity=35.1724137931034, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193206505, Length=435, Percent_Identity=23.448275862069, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996953, Length=150, Percent_Identity=31.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17541374, Length=148, Percent_Identity=31.7567567567568, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583025, Length=435, Percent_Identity=21.8390804597701, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137668, Length=435, Percent_Identity=21.8390804597701, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583023, Length=435, Percent_Identity=21.8390804597701, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45005; Mature: 45005

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCCCCC
>Mature Secondary Structure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HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]