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The map label for this gene is gcvH [H]

Identifier: 146302181

GI number: 146302181

Start: 5369651

End: 5370031

Strand: Direct

Name: gcvH [H]

Synonym: Fjoh_4450

Alternate gene names: 146302181

Gene position: 5369651-5370031 (Clockwise)

Preceding gene: 146302180

Following gene: 146302182

Centisome position: 88.07

GC content: 35.96

Gene sequence:

>381_bases
ATGGAAATACCTAAAAACCTTTTATACACACAAGACCACGAATGGGTAAAAATCGAAAATGATACAGCCTATATTGGAAT
TACTGATTTTGCACAGCACGAATTAGGTGATATTGTCTATGTTGAAATAGAAACCCTCGGGGAGAAATTAAGCCAGCATG
AGGTATTTGGTACAGTTGAAGCTGTAAAAACGGTATCTGACCTTTTCCTTCCGCTTACAGGGGAAATTCTGGAATTGAAT
CCTAAATTAGAAACAAATCCCGAATTGGTCAATTCCGATCCTTATGATGAGGGCTGGATGATTAAAATAAAAATTGAAAA
CAATACAGAAATTGATGCATTACTGAAATCCGATACCTACGCATCCTTAATAGGTTTATGA

Upstream 100 bases:

>100_bases
AGTTTTTGAAATAACCGAGCCACATCAATGCTGCATCTATTGTGCATACGGGAATGTTCCGTGTCCGGAAAAACAAAACA
AAATTAAAAAACAATAAAAA

Downstream 100 bases:

>100_bases
TATGGAAAATCAAAGCGTAAATATCAAAAAAACAGCCCTTAATGCAATTCATCATGACCTTGGCGGGAAAATGACCCCAT
TTGCCGGTTATGAAATGCCG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MEIPKNLLYTQDHEWVKIENDTAYIGITDFAQHELGDIVYVEIETLGEKLSQHEVFGTVEAVKTVSDLFLPLTGEILELN
PKLETNPELVNSDPYDEGWMIKIKIENNTEIDALLKSDTYASLIGL

Sequences:

>Translated_126_residues
MEIPKNLLYTQDHEWVKIENDTAYIGITDFAQHELGDIVYVEIETLGEKLSQHEVFGTVEAVKTVSDLFLPLTGEILELN
PKLETNPELVNSDPYDEGWMIKIKIENNTEIDALLKSDTYASLIGL
>Mature_126_residues
MEIPKNLLYTQDHEWVKIENDTAYIGITDFAQHELGDIVYVEIETLGEKLSQHEVFGTVEAVKTVSDLFLPLTGEILELN
PKLETNPELVNSDPYDEGWMIKIKIENNTEIDALLKSDTYASLIGL

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=116, Percent_Identity=43.1034482758621, Blast_Score=117, Evalue=3e-27,
Organism=Homo sapiens, GI89057342, Length=116, Percent_Identity=42.2413793103448, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=40.650406504065, Blast_Score=110, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=40.5172413793103, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=38.9380530973451, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=38.8429752066116, Blast_Score=103, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=35.7723577235772, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14280; Mature: 14280

Theoretical pI: Translated: 3.94; Mature: 3.94

Prosite motif: PS00867 CPSASE_2 ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPKNLLYTQDHEWVKIENDTAYIGITDFAQHELGDIVYVEIETLGEKLSQHEVFGTVE
CCCCCCCEEECCCCEEEEECCEEEEEECHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
AVKTVSDLFLPLTGEILELNPKLETNPELVNSDPYDEGWMIKIKIENNTEIDALLKSDTY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCH
ASLIGL
HHHHCC
>Mature Secondary Structure
MEIPKNLLYTQDHEWVKIENDTAYIGITDFAQHELGDIVYVEIETLGEKLSQHEVFGTVE
CCCCCCCEEECCCCEEEEECCEEEEEECHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHH
AVKTVSDLFLPLTGEILELNPKLETNPELVNSDPYDEGWMIKIKIENNTEIDALLKSDTY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCH
ASLIGL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA