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The map label for this gene is helA [H]

Identifier: 146302169

GI number: 146302169

Start: 5359272

End: 5360171

Strand: Direct

Name: helA [H]

Synonym: Fjoh_4437

Alternate gene names: 146302169

Gene position: 5359272-5360171 (Clockwise)

Preceding gene: 146302159

Following gene: 146302170

Centisome position: 87.9

GC content: 37.0

Gene sequence:

>900_bases
GTGGAAGTTTCAATATTGATAAAAACGGCGAAAATTATCTTGGTTGAATCGTGCCACATTATACAAAAAAATAACAAGAC
AGATGAAAACGACGACGATCGGGATATTGTAATCACAACACCAAAAGACGAAAGGGCGACGTTAAATTCATTGGATAATG
TTTTTATAAATACTACAACCGGTAAAGCTGTTCCATTGTCACAGATTTCAACTTTAACCATGGAAAGTTCTCCTTTATTT
ATTAAGCATTTAAACAAAATAAGAACTGTTTCCATCACTGCTTTTGTTGATAAAAATCACCTGACAGACAATGTTATTAC
AGACTTAATGAGCAAATTGGATAATTTCAAATTTCCTTCAGGTTATAAATATACTATGGGAGGTGAGTTCGAGTCTAAAA
ATGAATTCTTTGGAGGTTTTGAAACCATTATTATAATTACCATTTTTCTCTTTGTTGCTGTTTTAATCTTACTATTCAAA
ACTTTTAAAAGAACACTGATTGTTTTATCGGTTATACCTTTGGGAATTGTGGGCGCTGTTACAGCCCTTTGGGTAACTGG
TAACTCCCTGTGTTTTGTGGCAACTATTGGTTTAATTGCCTTAGTGGGAATCGAAGTTAAGAACTCTATCTTATTTGTTG
ATTTTACTAATCAGCTCAGGATGCACGGAATGTCAGTGGAAGACGCTATACGTAAAGGCGGTGAAGTCCGTTTTCTGCCC
ATCATGTTGACTTCCCTGACAGCTATTGGTGGCCTGCTGCCTATTGCGCTGTCAACTAATCCGTTGATTTCCCCGCTGGC
AGTAGTGATGATAGGCGGACTGATTAGCTCAACGCTGCTATCAAGAATTGTGACACCGATAGTATACAAATTGATGCCCC
CGAGTATAAAAACACTGTAA

Upstream 100 bases:

>100_bases
TTTCAAAAAAATATTTGTCTTAGTTTGTTGTAAAAATGTATATCTATGTATCTGAATCAATTAATTATTATAACGAAATT
TTAAACGGATTTAAATCATT

Downstream 100 bases:

>100_bases
AATATTATCTTTTAAAATTAAGATAATTCTCCTGTTAGAACTGTATAATTTCGCAAGCCCTGATTGGTCTTGCGAAATTA
TCAAAGGAAGGGCCTAAAAC

Product: cation/multidrug efflux pump-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEVSILIKTAKIILVESCHIIQKNNKTDENDDDRDIVITTPKDERATLNSLDNVFINTTTGKAVPLSQISTLTMESSPLF
IKHLNKIRTVSITAFVDKNHLTDNVITDLMSKLDNFKFPSGYKYTMGGEFESKNEFFGGFETIIIITIFLFVAVLILLFK
TFKRTLIVLSVIPLGIVGAVTALWVTGNSLCFVATIGLIALVGIEVKNSILFVDFTNQLRMHGMSVEDAIRKGGEVRFLP
IMLTSLTAIGGLLPIALSTNPLISPLAVVMIGGLISSTLLSRIVTPIVYKLMPPSIKTL

Sequences:

>Translated_299_residues
MEVSILIKTAKIILVESCHIIQKNNKTDENDDDRDIVITTPKDERATLNSLDNVFINTTTGKAVPLSQISTLTMESSPLF
IKHLNKIRTVSITAFVDKNHLTDNVITDLMSKLDNFKFPSGYKYTMGGEFESKNEFFGGFETIIIITIFLFVAVLILLFK
TFKRTLIVLSVIPLGIVGAVTALWVTGNSLCFVATIGLIALVGIEVKNSILFVDFTNQLRMHGMSVEDAIRKGGEVRFLP
IMLTSLTAIGGLLPIALSTNPLISPLAVVMIGGLISSTLLSRIVTPIVYKLMPPSIKTL
>Mature_299_residues
MEVSILIKTAKIILVESCHIIQKNNKTDENDDDRDIVITTPKDERATLNSLDNVFINTTTGKAVPLSQISTLTMESSPLF
IKHLNKIRTVSITAFVDKNHLTDNVITDLMSKLDNFKFPSGYKYTMGGEFESKNEFFGGFETIIIITIFLFVAVLILLFK
TFKRTLIVLSVIPLGIVGAVTALWVTGNSLCFVATIGLIALVGIEVKNSILFVDFTNQLRMHGMSVEDAIRKGGEVRFLP
IMLTSLTAIGGLLPIALSTNPLISPLAVVMIGGLISSTLLSRIVTPIVYKLMPPSIKTL

Specific function: Presumed to function with HelC and HelB in efflux of an unidentified substrate [H]

COG id: COG0841

COG function: function code V; Cation/multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1788391, Length=259, Percent_Identity=32.046332046332, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788390, Length=248, Percent_Identity=32.6612903225806, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788814, Length=245, Percent_Identity=28.1632653061224, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786788, Length=267, Percent_Identity=23.9700374531835, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786667, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789666, Length=241, Percent_Identity=27.8008298755187, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789930, Length=248, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR004763 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 32774; Mature: 32774

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVSILIKTAKIILVESCHIIQKNNKTDENDDDRDIVITTPKDERATLNSLDNVFINTTT
CCEEEEEEEEEEEEEECHHEEECCCCCCCCCCCCEEEEECCCCCHHHHHCCCCEEEECCC
GKAVPLSQISTLTMESSPLFIKHLNKIRTVSITAFVDKNHLTDNVITDLMSKLDNFKFPS
CCCCCHHHHHEEEECCCCHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCC
GYKYTMGGEFESKNEFFGGFETIIIITIFLFVAVLILLFKTFKRTLIVLSVIPLGIVGAV
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALWVTGNSLCFVATIGLIALVGIEVKNSILFVDFTNQLRMHGMSVEDAIRKGGEVRFLP
HHHHHCCCCCHHHHHHHHHHHHCEEECCCEEEEEECCHHHHCCCCHHHHHHCCCCEEEHH
IMLTSLTAIGGLLPIALSTNPLISPLAVVMIGGLISSTLLSRIVTPIVYKLMPPSIKTL
HHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEVSILIKTAKIILVESCHIIQKNNKTDENDDDRDIVITTPKDERATLNSLDNVFINTTT
CCEEEEEEEEEEEEEECHHEEECCCCCCCCCCCCEEEEECCCCCHHHHHCCCCEEEECCC
GKAVPLSQISTLTMESSPLFIKHLNKIRTVSITAFVDKNHLTDNVITDLMSKLDNFKFPS
CCCCCHHHHHEEEECCCCHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHHHHCCCCCC
GYKYTMGGEFESKNEFFGGFETIIIITIFLFVAVLILLFKTFKRTLIVLSVIPLGIVGAV
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALWVTGNSLCFVATIGLIALVGIEVKNSILFVDFTNQLRMHGMSVEDAIRKGGEVRFLP
HHHHHCCCCCHHHHHHHHHHHHCEEECCCEEEEEECCHHHHCCCCHHHHHHCCCCEEEHH
IMLTSLTAIGGLLPIALSTNPLISPLAVVMIGGLISSTLLSRIVTPIVYKLMPPSIKTL
HHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8613357 [H]