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The map label for this gene is oprM [H]

Identifier: 146302143

GI number: 146302143

Start: 5319875

End: 5321311

Strand: Direct

Name: oprM [H]

Synonym: Fjoh_4409

Alternate gene names: 146302143

Gene position: 5319875-5321311 (Clockwise)

Preceding gene: 146302142

Following gene: 146302144

Centisome position: 87.26

GC content: 40.08

Gene sequence:

>1437_bases
ATGAAGAATAACAGAAATATAAAATACGCAGGCCTAGCCTGCTTATTGCTCTCTATAGCGGGCTGTAAAACGACGACTAT
TGCAGAAAAAACAGAAAACAAAACAGTGCCAGAAAACTATGCCGATAATGGCTTGGACACTCTTAATACTGGAAAAGTAA
AATGGAGAAGCTATTTTACTGATGAAAATTTAGAAAACCTGATTGATATTGCGCTCAAGAACAATCAGGAATTAAATAGT
ACGCTTCAGGAAATCGAAATTGCAAGAAGCGAAGTAAAAGCCCGAAAAGGAGAATATCTGCCTTTTGTTGGCTTTAGCGC
TGGTGCAGATGTAACCAAATCCGGAAGATACACAAGCACAGGTTCAGGCGAGGCAACAACAGAGATTATGCCGGGCAGAG
AAACGCCTGATCCGCTTCCCAATTACCGTTTTGCACTGACTTCAAGCTGGGAAATCGATATTTGGAACAAGCTGCATACT
GCCAAAAAAGCCGCTTTAAACCGTTATTTGGGCTCTATTGAAGGAAAGAATTTCGTAGTCACCAATCTGATTGCAGAAAT
AGCCGATTCGTATTACGAACTTTTAGCGTTGGATAATCAATTGGAAATCATCAGACAAAATATTGATATTCAAACGAATG
CCTTAAAAGTCGTAAAAATACAAAAAGAGGCGGCAAGGGTAAACGAACTGGCTGTGCGCCGGTTTGAGGCTCAGATTTTA
AGCACGCAAAGCCTTCAGTTTGGCATACAGCAGCAGATAACCGAAACAGAAAATAAAATCAATTTCCTCTTGGGGCGTTT
TCCTCAAAAAATTGCCAGAAACAGCAATACATTCAGTTCTATAACTCCTGCAGATATTCATGCCGGAGTGCCTTCTCAGC
TTCTGGAAAATCGTCCCGACATTAAAAAAGCAGAAATGGAACTAATCGCTGCAAAACTCGACATTAAGGCCGCTAAAGCT
AAATTTTATCCATCGCTGGGCATTTCAGCAGGAATTGGTTATGAAGCTTTCAATGCCAAGTATCTGCTGACTTCTCCAGA
ATCTCTATTGTATTCCATAGCCGGAGATTTGGTTGCTCCTTTGATTAACAGAAATGCCATTAAAGCAGAATATCTTACCG
CTGGCGCCAAACAGATCCAGGCAGTCTACAATTATGAAAGGACCCTGGTAAATGCTTACGTTGAAGTGGCAAACCAACTG
TCTAAAATTCAAAATACGGCAAAAAGCTATGATTTGAAGTCAAAACAGGTTGAGGCTTTAAACGAATCTGTTCAGATATC
CACTAACTTGTTTAGTTCTGCCAGGGCAGATTATATGGAAGTTTTACTGACCCAGAAAGATGCTTTAGAATCTAAGTTTG
AACTTATCGAAACCAAAAAACAGCAGATGAATGCTTTTGTAAATATTTACAAAGCATTAGGCGGCGGGTGGAATTAG

Upstream 100 bases:

>100_bases
CAGGGCCGAAAACTAATTAAAGGAGAAGAAGAAAGTTCATTATCGGATGATTTCATGCATCATATTGATGATTTTTCAAC
AAATAATCAAACACGGGAAA

Downstream 100 bases:

>100_bases
TAGTTTTCATTTTTATTTGATTTTTTGAAAAGGAGACCTGTTTTTTAACAGGGCTCCTTTTCTGCTTACCACCTAGTCCT
GGATCAAATATAAAACTAAT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKNNRNIKYAGLACLLLSIAGCKTTTIAEKTENKTVPENYADNGLDTLNTGKVKWRSYFTDENLENLIDIALKNNQELNS
TLQEIEIARSEVKARKGEYLPFVGFSAGADVTKSGRYTSTGSGEATTEIMPGRETPDPLPNYRFALTSSWEIDIWNKLHT
AKKAALNRYLGSIEGKNFVVTNLIAEIADSYYELLALDNQLEIIRQNIDIQTNALKVVKIQKEAARVNELAVRRFEAQIL
STQSLQFGIQQQITETENKINFLLGRFPQKIARNSNTFSSITPADIHAGVPSQLLENRPDIKKAEMELIAAKLDIKAAKA
KFYPSLGISAGIGYEAFNAKYLLTSPESLLYSIAGDLVAPLINRNAIKAEYLTAGAKQIQAVYNYERTLVNAYVEVANQL
SKIQNTAKSYDLKSKQVEALNESVQISTNLFSSARADYMEVLLTQKDALESKFELIETKKQQMNAFVNIYKALGGGWN

Sequences:

>Translated_478_residues
MKNNRNIKYAGLACLLLSIAGCKTTTIAEKTENKTVPENYADNGLDTLNTGKVKWRSYFTDENLENLIDIALKNNQELNS
TLQEIEIARSEVKARKGEYLPFVGFSAGADVTKSGRYTSTGSGEATTEIMPGRETPDPLPNYRFALTSSWEIDIWNKLHT
AKKAALNRYLGSIEGKNFVVTNLIAEIADSYYELLALDNQLEIIRQNIDIQTNALKVVKIQKEAARVNELAVRRFEAQIL
STQSLQFGIQQQITETENKINFLLGRFPQKIARNSNTFSSITPADIHAGVPSQLLENRPDIKKAEMELIAAKLDIKAAKA
KFYPSLGISAGIGYEAFNAKYLLTSPESLLYSIAGDLVAPLINRNAIKAEYLTAGAKQIQAVYNYERTLVNAYVEVANQL
SKIQNTAKSYDLKSKQVEALNESVQISTNLFSSARADYMEVLLTQKDALESKFELIETKKQQMNAFVNIYKALGGGWN
>Mature_478_residues
MKNNRNIKYAGLACLLLSIAGCKTTTIAEKTENKTVPENYADNGLDTLNTGKVKWRSYFTDENLENLIDIALKNNQELNS
TLQEIEIARSEVKARKGEYLPFVGFSAGADVTKSGRYTSTGSGEATTEIMPGRETPDPLPNYRFALTSSWEIDIWNKLHT
AKKAALNRYLGSIEGKNFVVTNLIAEIADSYYELLALDNQLEIIRQNIDIQTNALKVVKIQKEAARVNELAVRRFEAQIL
STQSLQFGIQQQITETENKINFLLGRFPQKIARNSNTFSSITPADIHAGVPSQLLENRPDIKKAEMELIAAKLDIKAAKA
KFYPSLGISAGIGYEAFNAKYLLTSPESLLYSIAGDLVAPLINRNAIKAEYLTAGAKQIQAVYNYERTLVNAYVEVANQL
SKIQNTAKSYDLKSKQVEALNESVQISTNLFSSARADYMEVLLTQKDALESKFELIETKKQQMNAFVNIYKALGGGWN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=470, Percent_Identity=25.531914893617, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI2367348, Length=491, Percent_Identity=21.3849287169043, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53200; Mature: 53200

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNRNIKYAGLACLLLSIAGCKTTTIAEKTENKTVPENYADNGLDTLNTGKVKWRSYFT
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
DENLENLIDIALKNNQELNSTLQEIEIARSEVKARKGEYLPFVGFSAGADVTKSGRYTST
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCC
GSGEATTEIMPGRETPDPLPNYRFALTSSWEIDIWNKLHTAKKAALNRYLGSIEGKNFVV
CCCCCEEEECCCCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHH
TNLIAEIADSYYELLALDNQLEIIRQNIDIQTNALKVVKIQKEAARVNELAVRRFEAQIL
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
STQSLQFGIQQQITETENKINFLLGRFPQKIARNSNTFSSITPADIHAGVPSQLLENRPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHCCCCC
IKKAEMELIAAKLDIKAAKAKFYPSLGISAGIGYEAFNAKYLLTSPESLLYSIAGDLVAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHH
LINRNAIKAEYLTAGAKQIQAVYNYERTLVNAYVEVANQLSKIQNTAKSYDLKSKQVEAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NESVQISTNLFSSARADYMEVLLTQKDALESKFELIETKKQQMNAFVNIYKALGGGWN
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNNRNIKYAGLACLLLSIAGCKTTTIAEKTENKTVPENYADNGLDTLNTGKVKWRSYFT
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
DENLENLIDIALKNNQELNSTLQEIEIARSEVKARKGEYLPFVGFSAGADVTKSGRYTST
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCC
GSGEATTEIMPGRETPDPLPNYRFALTSSWEIDIWNKLHTAKKAALNRYLGSIEGKNFVV
CCCCCEEEECCCCCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHH
TNLIAEIADSYYELLALDNQLEIIRQNIDIQTNALKVVKIQKEAARVNELAVRRFEAQIL
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHH
STQSLQFGIQQQITETENKINFLLGRFPQKIARNSNTFSSITPADIHAGVPSQLLENRPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHCCCCC
IKKAEMELIAAKLDIKAAKAKFYPSLGISAGIGYEAFNAKYLLTSPESLLYSIAGDLVAP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHH
LINRNAIKAEYLTAGAKQIQAVYNYERTLVNAYVEVANQLSKIQNTAKSYDLKSKQVEAL
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NESVQISTNLFSSARADYMEVLLTQKDALESKFELIETKKQQMNAFVNIYKALGGGWN
HHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]