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The map label for this gene is czcB [H]

Identifier: 146302030

GI number: 146302030

Start: 5187400

End: 5188485

Strand: Direct

Name: czcB [H]

Synonym: Fjoh_4294

Alternate gene names: 146302030

Gene position: 5187400-5188485 (Clockwise)

Preceding gene: 146302029

Following gene: 146302031

Centisome position: 85.08

GC content: 36.37

Gene sequence:

>1086_bases
ATGAAGAAACTTATAATCCCAATACTTGGGTTAATGCTCGTTTACGGATGCGGCAAGAAAGAAGAAGTAAAAAAAGCTCA
GGATGAAAAATTCTGCATCGATAAAGATTTAAAAGAAAAAATTACAATAGAACCTGTACAGAAACGTGCCGTGAGCGAAT
CTATTAACCTTACCGGAAATATTACTTACAACGGCGATCACGTGGTTCAGTTTAACAGCCTTGTTGAAGGAATTATTACC
AAAACTACATTTTCACTAGGCGATTATGTAAAAAAAGGACAAGTTCTGGCAGAGATAAAAAGTACAGAACTAAACAGCAT
GCAGTCTGAAAGTAAATCATTGCAGTCGCAGATAACGGTAGCACAGCGCAATTTACAAGCCACAAAATCAATGTTTGAAG
ACGGAATTTCTTCTCAAAAAGACTTATTGCAGGCACAAAGCGAACTGGATGTTTTAAAATCTTCTTTAGAAAACGTAAGA
GCCAATCTGGCAATGTTCAGCGCAAGCAGTGAAAGAGCTGTTTTTCAGATCAAAGCGCCGACTGAAGGTTTTATTGTAGA
TAAAAATATCAGCCCGGGAATGCAGATTACAAACGAAAGCGATCCATTGTTCACCATTTCTGATTTAAAAGAAATCTGGG
TTTTGGTAAATGTTTATACCAGTAATCTTAAAAACATCAGCGAAAACATGCAGGTCGATGTAACGACACCTGCTTATCCT
GGAGAAATTTTTAAAGGAAAAATCAATATGATGTCTAAAGTTTTTGATGCCGATGAACACGTTTTAAAAGCACGAATTGT
AATGGACAACCGAAACATGAAATTAAAACCGGGAATGACAGCCGATATTACAATCGATAAAAGCAAAGGCGGTGAAGAAC
TGGCAGCTATTCCTGCAAAAGCAGCTATTTTCGACAACAACCGCGATTATATTTTAATCTACAAAAACGATTGCACAATC
GAAACCAGAGAAATAAACCCCGTTTTAAAAAACAACAGCTGGCTTTATTTTGATAAAGGTGTAAATGAAGGCGAAAAGGT
AATTACCAAAAACCAGCTCCTGATTTACGAAAGATTAAAAAACTAA

Upstream 100 bases:

>100_bases
CTCAATTCGAAAAAAGACCTGAATAAAAATTTCGAAGAACTGCGCTATATCACGGGGCAGGAAATCAATTAAAACATCAA
TTTCTCAAAACAAAAAGATA

Downstream 100 bases:

>100_bases
GCATCCTTAAAAACGGATAAACACAATACAAGACATGAAAAAATTTGTACAAGGTCTGGTTGCTTTTTCCTTGAAAAACT
CGCTTATTATATTCTTTTTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKKLIIPILGLMLVYGCGKKEEVKKAQDEKFCIDKDLKEKITIEPVQKRAVSESINLTGNITYNGDHVVQFNSLVEGIIT
KTTFSLGDYVKKGQVLAEIKSTELNSMQSESKSLQSQITVAQRNLQATKSMFEDGISSQKDLLQAQSELDVLKSSLENVR
ANLAMFSASSERAVFQIKAPTEGFIVDKNISPGMQITNESDPLFTISDLKEIWVLVNVYTSNLKNISENMQVDVTTPAYP
GEIFKGKINMMSKVFDADEHVLKARIVMDNRNMKLKPGMTADITIDKSKGGEELAAIPAKAAIFDNNRDYILIYKNDCTI
ETREINPVLKNNSWLYFDKGVNEGEKVITKNQLLIYERLKN

Sequences:

>Translated_361_residues
MKKLIIPILGLMLVYGCGKKEEVKKAQDEKFCIDKDLKEKITIEPVQKRAVSESINLTGNITYNGDHVVQFNSLVEGIIT
KTTFSLGDYVKKGQVLAEIKSTELNSMQSESKSLQSQITVAQRNLQATKSMFEDGISSQKDLLQAQSELDVLKSSLENVR
ANLAMFSASSERAVFQIKAPTEGFIVDKNISPGMQITNESDPLFTISDLKEIWVLVNVYTSNLKNISENMQVDVTTPAYP
GEIFKGKINMMSKVFDADEHVLKARIVMDNRNMKLKPGMTADITIDKSKGGEELAAIPAKAAIFDNNRDYILIYKNDCTI
ETREINPVLKNNSWLYFDKGVNEGEKVITKNQLLIYERLKN
>Mature_361_residues
MKKLIIPILGLMLVYGCGKKEEVKKAQDEKFCIDKDLKEKITIEPVQKRAVSESINLTGNITYNGDHVVQFNSLVEGIIT
KTTFSLGDYVKKGQVLAEIKSTELNSMQSESKSLQSQITVAQRNLQATKSMFEDGISSQKDLLQAQSELDVLKSSLENVR
ANLAMFSASSERAVFQIKAPTEGFIVDKNISPGMQITNESDPLFTISDLKEIWVLVNVYTSNLKNISENMQVDVTTPAYP
GEIFKGKINMMSKVFDADEHVLKARIVMDNRNMKLKPGMTADITIDKSKGGEELAAIPAKAAIFDNNRDYILIYKNDCTI
ETREINPVLKNNSWLYFDKGVNEGEKVITKNQLLIYERLKN

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=301, Percent_Identity=21.9269102990033, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40493; Mature: 40493

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIIPILGLMLVYGCGKKEEVKKAQDEKFCIDKDLKEKITIEPVQKRAVSESINLTGN
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHCCCCEEEE
ITYNGDHVVQFNSLVEGIITKTTFSLGDYVKKGQVLAEIKSTELNSMQSESKSLQSQITV
EEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHH
AQRNLQATKSMFEDGISSQKDLLQAQSELDVLKSSLENVRANLAMFSASSERAVFQIKAP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECC
TEGFIVDKNISPGMQITNESDPLFTISDLKEIWVLVNVYTSNLKNISENMQVDVTTPAYP
CCCEEEECCCCCCCEEECCCCCEEEHHHHHHHHEEHHHHHHHHHHCCCCCEEEEECCCCC
GEIFKGKINMMSKVFDADEHVLKARIVMDNRNMKLKPGMTADITIDKSKGGEELAAIPAK
CHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCEEECCCCEEEEEEECCCCCCHHHHCCCE
AAIFDNNRDYILIYKNDCTIETREINPVLKNNSWLYFDKGVNEGEKVITKNQLLIYERLK
EEEEECCCCEEEEEECCCEEEEEECCEEEECCCEEEEECCCCCCCCEEECCCEEEEEECC
N
C
>Mature Secondary Structure
MKKLIIPILGLMLVYGCGKKEEVKKAQDEKFCIDKDLKEKITIEPVQKRAVSESINLTGN
CCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHCCCCCHHHHHHHHCCCCEEEE
ITYNGDHVVQFNSLVEGIITKTTFSLGDYVKKGQVLAEIKSTELNSMQSESKSLQSQITV
EEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHH
AQRNLQATKSMFEDGISSQKDLLQAQSELDVLKSSLENVRANLAMFSASSERAVFQIKAP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECC
TEGFIVDKNISPGMQITNESDPLFTISDLKEIWVLVNVYTSNLKNISENMQVDVTTPAYP
CCCEEEECCCCCCCEEECCCCCEEEHHHHHHHHEEHHHHHHHHHHCCCCCEEEEECCCCC
GEIFKGKINMMSKVFDADEHVLKARIVMDNRNMKLKPGMTADITIDKSKGGEELAAIPAK
CHHHHHHHHHHHHHHCCHHHEEEEEEEEECCCEEECCCCEEEEEEECCCCCCHHHHCCCE
AAIFDNNRDYILIYKNDCTIETREINPVLKNNSWLYFDKGVNEGEKVITKNQLLIYERLK
EEEEECCCCEEEEEECCCEEEEEECCEEEECCCEEEEECCCCCCCCEEECCCEEEEEECC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]