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The map label for this gene is pucA [H]

Identifier: 146301941

GI number: 146301941

Start: 5056346

End: 5057485

Strand: Reverse

Name: pucA [H]

Synonym: Fjoh_4205

Alternate gene names: 146301941

Gene position: 5057485-5056346 (Counterclockwise)

Preceding gene: 146301942

Following gene: 146301940

Centisome position: 82.95

GC content: 36.75

Gene sequence:

>1140_bases
ATGAACATTATTTTTATGAAAGAAATCAGCGAAATTCTCAAAGCCTATTCACAAGCAAAATCGGAAGGAAAAAAAACAGC
TTTGGCTACAGTCGTTAAAGTTGAAGGATCTTCGTACCGACAGCCCGGTGCGCGCATGCTTGTTACCGAAGACGGCCAGC
TTACAGGAGCAATAAGCGGAGGCTGTCTGGAAGGAGATGCACTGCGAAAAGCACTGCTTTCCATTAATCAAAAACAAAAT
AAACTCATTACTTATAATACCAATAATGAAGATGATATTGAATTAGGATTGCAGCTTGGATGCAACGGAATTGTACATAT
TTTGTTTGAATATATAAATGATGAAATCCCTAATAATCCCATTCAGCTTTTACAACAACTGGAATTAGAAAGAAAAGAAG
CCGTAATAGTTACTGTTTTTTCTCTAAAAAGAAATGCCCATCAAATAGGAACGGCATTATTTTTTAGAAAAGAAAGTCCC
GTATTAAGCTGTAACAATGATATTCCAGATCTAATTTCTGATGTAAATGAAGTAATGCAGACCAAAACTTCTGCAGTAAA
AAAACTTCCGGGAGAAAACAGTACCGAAGCCCTGATAGAATACATAAAACCTGTTATTTCGCTCGTTATTGCCGGAGCAG
GAAACGATGTGCAGCCTTTAGTAAAAATGGCTTCAATTTTGGGCTGGAAAGTTATTATTGGCGACGGCCGCGCTACTCAC
GCTGTATCAAAACGTTTTCCAAAAGCAGATAAAGTAAATGCCGTAAAACCAGAACAGTTTTTAGAAGATATTCTAATCGA
CGATCAAACCTATTTTGTTTTAATGACACACAATTACAAATACGATTTAGCGGTTTTGAAAATGCTTTTTGAAACCAGCT
GTCAGTACATTGGAATCCTCGGCCCCAAAACCAAACTGCAGCGAATGCTCAATGATCTTCAAAATGAAGGAATAAAAATC
AGCGAAGAACAATTAAAACGCATTTACAGCCCAATTGGATTAGATATTGGAGCCGAAACTTCTGAAGAAATTGCTTTATC
AATTGTTTCAGAAATAAAAGCCTTTGCCACAGGAAAAACCGGAACTTCCCTGAAATTCAAAACCGGAAAAATTCACGATG
AAATTCATTATGGAGAATAA

Upstream 100 bases:

>100_bases
TTTAAATTCAAAATATTATAAACTTAAACACACTCGTTAAGTTTATAATATTTTTTTATTTTTAAAGTATGAAAGCGTCA
TCAATTCTAATTTCTTTTTG

Downstream 100 bases:

>100_bases
ATTTCACAATAAAACCGGTATTGTAATTTTAGCCGCCGGCAGTTCTTCCAGATTAGGACGTCCGAAACAATTGCTGCAAT
ACAACCAATCAACACTTTTA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: XDHase subunit A [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNIIFMKEISEILKAYSQAKSEGKKTALATVVKVEGSSYRQPGARMLVTEDGQLTGAISGGCLEGDALRKALLSINQKQN
KLITYNTNNEDDIELGLQLGCNGIVHILFEYINDEIPNNPIQLLQQLELERKEAVIVTVFSLKRNAHQIGTALFFRKESP
VLSCNNDIPDLISDVNEVMQTKTSAVKKLPGENSTEALIEYIKPVISLVIAGAGNDVQPLVKMASILGWKVIIGDGRATH
AVSKRFPKADKVNAVKPEQFLEDILIDDQTYFVLMTHNYKYDLAVLKMLFETSCQYIGILGPKTKLQRMLNDLQNEGIKI
SEEQLKRIYSPIGLDIGAETSEEIALSIVSEIKAFATGKTGTSLKFKTGKIHDEIHYGE

Sequences:

>Translated_379_residues
MNIIFMKEISEILKAYSQAKSEGKKTALATVVKVEGSSYRQPGARMLVTEDGQLTGAISGGCLEGDALRKALLSINQKQN
KLITYNTNNEDDIELGLQLGCNGIVHILFEYINDEIPNNPIQLLQQLELERKEAVIVTVFSLKRNAHQIGTALFFRKESP
VLSCNNDIPDLISDVNEVMQTKTSAVKKLPGENSTEALIEYIKPVISLVIAGAGNDVQPLVKMASILGWKVIIGDGRATH
AVSKRFPKADKVNAVKPEQFLEDILIDDQTYFVLMTHNYKYDLAVLKMLFETSCQYIGILGPKTKLQRMLNDLQNEGIKI
SEEQLKRIYSPIGLDIGAETSEEIALSIVSEIKAFATGKTGTSLKFKTGKIHDEIHYGE
>Mature_379_residues
MNIIFMKEISEILKAYSQAKSEGKKTALATVVKVEGSSYRQPGARMLVTEDGQLTGAISGGCLEGDALRKALLSINQKQN
KLITYNTNNEDDIELGLQLGCNGIVHILFEYINDEIPNNPIQLLQQLELERKEAVIVTVFSLKRNAHQIGTALFFRKESP
VLSCNNDIPDLISDVNEVMQTKTSAVKKLPGENSTEALIEYIKPVISLVIAGAGNDVQPLVKMASILGWKVIIGDGRATH
AVSKRFPKADKVNAVKPEQFLEDILIDDQTYFVLMTHNYKYDLAVLKMLFETSCQYIGILGPKTKLQRMLNDLQNEGIKI
SEEQLKRIYSPIGLDIGAETSEEIALSIVSEIKAFATGKTGTSLKFKTGKIHDEIHYGE

Specific function: Oxidizes hypoxanthine and xanthine to uric acid. PucA subunit could exert a molybdenum cofactor recruiting function [H]

COG id: COG1975

COG function: function code O; Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789240, Length=148, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003777 [H]

Pfam domain/function: PF02625 XdhC_CoxI [H]

EC number: =1.17.1.4 [H]

Molecular weight: Translated: 41944; Mature: 41944

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIFMKEISEILKAYSQAKSEGKKTALATVVKVEGSSYRQPGARMLVTEDGQLTGAISG
CCEEEHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCEEEEECCCCEEEECCC
GCLEGDALRKALLSINQKQNKLITYNTNNEDDIELGLQLGCNGIVHILFEYINDEIPNNP
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCH
IQLLQQLELERKEAVIVTVFSLKRNAHQIGTALFFRKESPVLSCNNDIPDLISDVNEVMQ
HHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHEEECCCCEEECCCCCHHHHHHHHHHHH
TKTSAVKKLPGENSTEALIEYIKPVISLVIAGAGNDVQPLVKMASILGWKVIIGDGRATH
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEECCCHHH
AVSKRFPKADKVNAVKPEQFLEDILIDDQTYFVLMTHNYKYDLAVLKMLFETSCQYIGIL
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEE
GPKTKLQRMLNDLQNEGIKISEEQLKRIYSPIGLDIGAETSEEIALSIVSEIKAFATGKT
CCHHHHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GTSLKFKTGKIHDEIHYGE
CCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNIIFMKEISEILKAYSQAKSEGKKTALATVVKVEGSSYRQPGARMLVTEDGQLTGAISG
CCEEEHHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCCCEEEEECCCCEEEECCC
GCLEGDALRKALLSINQKQNKLITYNTNNEDDIELGLQLGCNGIVHILFEYINDEIPNNP
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCH
IQLLQQLELERKEAVIVTVFSLKRNAHQIGTALFFRKESPVLSCNNDIPDLISDVNEVMQ
HHHHHHHHHHCCCEEEEEEEHHHCCHHHHHHHHEEECCCCEEECCCCCHHHHHHHHHHHH
TKTSAVKKLPGENSTEALIEYIKPVISLVIAGAGNDVQPLVKMASILGWKVIIGDGRATH
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEECCCHHH
AVSKRFPKADKVNAVKPEQFLEDILIDDQTYFVLMTHNYKYDLAVLKMLFETSCQYIGIL
HHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEE
GPKTKLQRMLNDLQNEGIKISEEQLKRIYSPIGLDIGAETSEEIALSIVSEIKAFATGKT
CCHHHHHHHHHHHHHCCCEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GTSLKFKTGKIHDEIHYGE
CCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]