Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 146301939

GI number: 146301939

Start: 5054558

End: 5055706

Strand: Direct

Name: iscS [H]

Synonym: Fjoh_4203

Alternate gene names: 146301939

Gene position: 5054558-5055706 (Clockwise)

Preceding gene: 146301935

Following gene: 146301943

Centisome position: 82.9

GC content: 36.55

Gene sequence:

>1149_bases
ATGCCGCAGCAGCAAAATATTTATCTGGACAATAATGCCACAACCCGTGTTGATGAACGCGTTCTCGAAGCTATGCTTCC
TTATTTTACAGATTTATATGCCAATTCTACAAGTCAGCATATTGCAGGATTAACGGTTAAGGAAGCAATTGAAAATGCTG
CGTGGCAAACTGCTGATTTAATAAATGCTGATGCAGAAGAAATTATTTTTACTTCTGGAGCAACTGAGGCAATCAATTTG
GCAATAAAAGGTCTGGCTGATCAAGAACGCAAACATATTGTTACTCTTGAAACAGAACATAAAGCAGTTCTTGAAACTTG
CCACTTTATGGAAAGTATCGGCTTTAATGCCAGCTATCTTCCTGTTGAACGTGACGGGCTTTTAGATCTTAATCTTTTAA
ATGAAACTGTTACAGATAAAACACTGGTTTTCATTGGAATGTTTTCTAATAACGAAACAGGTGTCATACAAAATGTCAGT
AAAATTTCTGCTATTTTAAAACGCAAAAATGTTCTTTTTTTGTGTGATACTACCCAGGCTGTTGGAAAAATTTCTATTGA
TGTAAAAGCTTTGGGAATTGATCTTTTAACAATGTCTGCTCATAAATTTTACGGACCAAAAGGAATTGGTGCTTTGTATC
TTTCGGCGAAAGCCAAAATAAAACTGCTTCCTCAGATTACTGGCGGCGGACATCAGAAAAAACTGCGAAGCGGTACACTG
AATGTTCCCGGAATTATTGGTTTAGGAAAAGCAGCAGAAATAGCTGTCAACGAATTAGAAAATGATAAAATCAGAATTGA
AAGACTTCGAAATGCATTAGAAAAAGGGTTATTGCAATTTAAAGGTTCTTTTGTAAACGGAAATATTCAGAACCGAATTT
ACAATACCACAAATATTTGTTTTCCCGGGGTAAATTCAGAACAGTTAATTATGGCTTTGGGGAATATCTCGGTTTCGAAT
GGAGCGTCGTGTTCGGCGGTTACTTCTGAGCCTTCACATGTTTTAAAAGCAATGGGATTGTCTGATGAAGAAGCTTTGAG
TTCGATTCGTTTTAGTCTGGGAAGATTTACTACGGAAGAAGAAATTGACATTGCTGTTGAACGTATTTTGACTTCAGCTG
AGCAATTAGTTTTGTCTCTAAAACGCTAA

Upstream 100 bases:

>100_bases
ATCCAGCTTATACAAATCTTAGCGTCCTTCGGTTAAGAAAAATTATGCACATTTGTTTTTACATTGAAAATTGTACTTTT
ACAAGACTAAGTTTTTATTT

Downstream 100 bases:

>100_bases
GTTTTTATTAAGCCTCAATTAATTCAACCTTTGCTCCTTTGCCCCTCTCAAAAAATAAGCTTATTGTAATCTTCTTCAGT
ATCAATATCGATACTGCCTT

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MPQQQNIYLDNNATTRVDERVLEAMLPYFTDLYANSTSQHIAGLTVKEAIENAAWQTADLINADAEEIIFTSGATEAINL
AIKGLADQERKHIVTLETEHKAVLETCHFMESIGFNASYLPVERDGLLDLNLLNETVTDKTLVFIGMFSNNETGVIQNVS
KISAILKRKNVLFLCDTTQAVGKISIDVKALGIDLLTMSAHKFYGPKGIGALYLSAKAKIKLLPQITGGGHQKKLRSGTL
NVPGIIGLGKAAEIAVNELENDKIRIERLRNALEKGLLQFKGSFVNGNIQNRIYNTTNICFPGVNSEQLIMALGNISVSN
GASCSAVTSEPSHVLKAMGLSDEEALSSIRFSLGRFTTEEEIDIAVERILTSAEQLVLSLKR

Sequences:

>Translated_382_residues
MPQQQNIYLDNNATTRVDERVLEAMLPYFTDLYANSTSQHIAGLTVKEAIENAAWQTADLINADAEEIIFTSGATEAINL
AIKGLADQERKHIVTLETEHKAVLETCHFMESIGFNASYLPVERDGLLDLNLLNETVTDKTLVFIGMFSNNETGVIQNVS
KISAILKRKNVLFLCDTTQAVGKISIDVKALGIDLLTMSAHKFYGPKGIGALYLSAKAKIKLLPQITGGGHQKKLRSGTL
NVPGIIGLGKAAEIAVNELENDKIRIERLRNALEKGLLQFKGSFVNGNIQNRIYNTTNICFPGVNSEQLIMALGNISVSN
GASCSAVTSEPSHVLKAMGLSDEEALSSIRFSLGRFTTEEEIDIAVERILTSAEQLVLSLKR
>Mature_381_residues
PQQQNIYLDNNATTRVDERVLEAMLPYFTDLYANSTSQHIAGLTVKEAIENAAWQTADLINADAEEIIFTSGATEAINLA
IKGLADQERKHIVTLETEHKAVLETCHFMESIGFNASYLPVERDGLLDLNLLNETVTDKTLVFIGMFSNNETGVIQNVSK
ISAILKRKNVLFLCDTTQAVGKISIDVKALGIDLLTMSAHKFYGPKGIGALYLSAKAKIKLLPQITGGGHQKKLRSGTLN
VPGIIGLGKAAEIAVNELENDKIRIERLRNALEKGLLQFKGSFVNGNIQNRIYNTTNICFPGVNSEQLIMALGNISVSNG
ASCSAVTSEPSHVLKAMGLSDEEALSSIRFSLGRFTTEEEIDIAVERILTSAEQLVLSLKR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=380, Percent_Identity=42.1052631578947, Blast_Score=323, Evalue=1e-88,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=31.8840579710145, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI48994898, Length=376, Percent_Identity=45.7446808510638, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1789175, Length=398, Percent_Identity=25.8793969849246, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787970, Length=268, Percent_Identity=28.7313432835821, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25143064, Length=378, Percent_Identity=43.3862433862434, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI17533177, Length=336, Percent_Identity=32.7380952380952, Blast_Score=150, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6319831, Length=369, Percent_Identity=44.9864498644986, Blast_Score=325, Evalue=1e-89,
Organism=Drosophila melanogaster, GI20129463, Length=375, Percent_Identity=43.7333333333333, Blast_Score=327, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41664; Mature: 41533

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQQNIYLDNNATTRVDERVLEAMLPYFTDLYANSTSQHIAGLTVKEAIENAAWQTADL
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHH
INADAEEIIFTSGATEAINLAIKGLADQERKHIVTLETEHKAVLETCHFMESIGFNASYL
HCCCHHHEEEECCCCCEEHHEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEC
PVERDGLLDLNLLNETVTDKTLVFIGMFSNNETGVIQNVSKISAILKRKNVLFLCDTTQA
EECCCCEEEEEHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCC
VGKISIDVKALGIDLLTMSAHKFYGPKGIGALYLSAKAKIKLLPQITGGGHQKKLRSGTL
CEEEEEEEEEECEEEEEECHHHHCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHHCCCC
NVPGIIGLGKAAEIAVNELENDKIRIERLRNALEKGLLQFKGSFVNGNIQNRIYNTTNIC
CCCCEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHEEECCCCEE
FPGVNSEQLIMALGNISVSNGASCSAVTSEPSHVLKAMGLSDEEALSSIRFSLGRFTTEE
ECCCCCCEEEEEECCEEECCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCH
EIDIAVERILTSAEQLVLSLKR
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQQQNIYLDNNATTRVDERVLEAMLPYFTDLYANSTSQHIAGLTVKEAIENAAWQTADL
CCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHH
INADAEEIIFTSGATEAINLAIKGLADQERKHIVTLETEHKAVLETCHFMESIGFNASYL
HCCCHHHEEEECCCCCEEHHEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCEEC
PVERDGLLDLNLLNETVTDKTLVFIGMFSNNETGVIQNVSKISAILKRKNVLFLCDTTQA
EECCCCEEEEEHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCC
VGKISIDVKALGIDLLTMSAHKFYGPKGIGALYLSAKAKIKLLPQITGGGHQKKLRSGTL
CEEEEEEEEEECEEEEEECHHHHCCCCCCEEEEEECCCEEEEEEEECCCCCHHHHHCCCC
NVPGIIGLGKAAEIAVNELENDKIRIERLRNALEKGLLQFKGSFVNGNIQNRIYNTTNIC
CCCCEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEECCCHHHEEECCCCEE
FPGVNSEQLIMALGNISVSNGASCSAVTSEPSHVLKAMGLSDEEALSSIRFSLGRFTTEE
ECCCCCCEEEEEECCEEECCCCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCH
EIDIAVERILTSAEQLVLSLKR
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA