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The map label for this gene is purR [H]

Identifier: 146301778

GI number: 146301778

Start: 4822581

End: 4823597

Strand: Direct

Name: purR [H]

Synonym: Fjoh_4042

Alternate gene names: 146301778

Gene position: 4822581-4823597 (Clockwise)

Preceding gene: 146301777

Following gene: 146301779

Centisome position: 79.1

GC content: 36.09

Gene sequence:

>1017_bases
GTGAAAAGAATTTCAATTAAAGATGTGGCATTAAAAGCAGGCGTATCTATCGCCTCAGTATCTTATGTACTTAACAATAA
GATTGACAGCCGTATTCCGCAGGAAACAATTGACAAGGTAAAACACGCTGCCGAAGAATTAAATTACCGTCCTAACAATA
TTGCAAAAAGCTTAAAAACACAGCGAACCCATATAATTGGTCTTGTTGTCGCTGATATTGCAAATCCTTACTTTTCTCAG
TTAGCCCGAATTATTGAAGATGAAGCCAACAAACTGGGTTATACCCTTTTAATTGGAAGCTCTGATGAAAATGCCGAAAA
ATTTGAAGCGCTTATCAATATGTTTGCCGACCGTCAGGCCGATGGATTAATTATTGCTCCTGTTGAAAACAGTGAAAAAT
GTTTGTTGAAACTGCAAAAGCAAAAGATTCCTTTTGTATTAATTGACCGTTATTTATCTGGTGTTGAAGCAGACAGCATA
CAAATTAATAACTACGATATAAGCCAAAAAGTTACAGAACACCTTATAAACGGCGGTTATAAAAACACTCTTCTTGTTGC
TTATAAAACACAGTTGCAGCACTTATTACAGCGAACTCAGGCATTTTTAAATACGCTCGAAAAACATCAAATACAAAACA
GTCAAGTGCTTTTAGCAGACAAAGACTATATTGACAGAGACATTAATACCGGCTTAGAAAATATGCTGCAAAATGCAGTA
AAACCAGATGCCATCTTTTTTACTAGTAATAAACTGGCAGTTTCCGGACTCAAAAAACTTACACAACTTGGCATAAATAT
TCCGCAGGATATTGCAGTTGCTGCTTTTGATGAAACTGAAGCTTATGAGCTTTTTCCGGTTCCGCTAACGTATGTAAAAC
AGCCGCTTTCTGCCATTGGGACAACCGCTGTAAAAATGCTTAACGAAAAAATCAATATCAAAAATCTGCCTTTGCAGAAT
GTAACTTTACATGCTGAACTTCATATAGGTCAGTCTACAAAATCTAAACACATCTAA

Upstream 100 bases:

>100_bases
GCTAAAATGATTTTTTTAACTCACTTAATTTACAAATTTCAAAATAAATACTTGCTTAAACGTTTAAGTTGATTATATTT
GCCTTTCTGAAATCGTTATC

Downstream 100 bases:

>100_bases
ACTTTTTTATTTACCACTTTGCTTAAACGTTTAATCAAAAAAAAAGAAAATCATGAGAGAAGAAATAAATACAAAATCAA
TCTATTGTTATGGAGAAGTA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MKRISIKDVALKAGVSIASVSYVLNNKIDSRIPQETIDKVKHAAEELNYRPNNIAKSLKTQRTHIIGLVVADIANPYFSQ
LARIIEDEANKLGYTLLIGSSDENAEKFEALINMFADRQADGLIIAPVENSEKCLLKLQKQKIPFVLIDRYLSGVEADSI
QINNYDISQKVTEHLINGGYKNTLLVAYKTQLQHLLQRTQAFLNTLEKHQIQNSQVLLADKDYIDRDINTGLENMLQNAV
KPDAIFFTSNKLAVSGLKKLTQLGINIPQDIAVAAFDETEAYELFPVPLTYVKQPLSAIGTTAVKMLNEKINIKNLPLQN
VTLHAELHIGQSTKSKHI

Sequences:

>Translated_338_residues
MKRISIKDVALKAGVSIASVSYVLNNKIDSRIPQETIDKVKHAAEELNYRPNNIAKSLKTQRTHIIGLVVADIANPYFSQ
LARIIEDEANKLGYTLLIGSSDENAEKFEALINMFADRQADGLIIAPVENSEKCLLKLQKQKIPFVLIDRYLSGVEADSI
QINNYDISQKVTEHLINGGYKNTLLVAYKTQLQHLLQRTQAFLNTLEKHQIQNSQVLLADKDYIDRDINTGLENMLQNAV
KPDAIFFTSNKLAVSGLKKLTQLGINIPQDIAVAAFDETEAYELFPVPLTYVKQPLSAIGTTAVKMLNEKINIKNLPLQN
VTLHAELHIGQSTKSKHI
>Mature_338_residues
MKRISIKDVALKAGVSIASVSYVLNNKIDSRIPQETIDKVKHAAEELNYRPNNIAKSLKTQRTHIIGLVVADIANPYFSQ
LARIIEDEANKLGYTLLIGSSDENAEKFEALINMFADRQADGLIIAPVENSEKCLLKLQKQKIPFVLIDRYLSGVEADSI
QINNYDISQKVTEHLINGGYKNTLLVAYKTQLQHLLQRTQAFLNTLEKHQIQNSQVLLADKDYIDRDINTGLENMLQNAV
KPDAIFFTSNKLAVSGLKKLTQLGINIPQDIAVAAFDETEAYELFPVPLTYVKQPLSAIGTTAVKMLNEKINIKNLPLQN
VTLHAELHIGQSTKSKHI

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=325, Percent_Identity=27.3846153846154, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=25.6797583081571, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1790194, Length=327, Percent_Identity=28.7461773700306, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789202, Length=313, Percent_Identity=26.8370607028754, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=26.6025641025641, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787906, Length=338, Percent_Identity=24.2603550295858, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788474, Length=303, Percent_Identity=26.0726072607261, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789068, Length=301, Percent_Identity=27.5747508305648, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786268, Length=294, Percent_Identity=27.5510204081633, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787580, Length=327, Percent_Identity=26.605504587156, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI48994940, Length=335, Percent_Identity=21.1940298507463, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789456, Length=326, Percent_Identity=24.8466257668712, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790715, Length=318, Percent_Identity=24.2138364779874, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37756; Mature: 37756

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRISIKDVALKAGVSIASVSYVLNNKIDSRIPQETIDKVKHAAEELNYRPNNIAKSLKT
CCCCCHHHHHHHCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
QRTHIIGLVVADIANPYFSQLARIIEDEANKLGYTLLIGSSDENAEKFEALINMFADRQA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCC
DGLIIAPVENSEKCLLKLQKQKIPFVLIDRYLSGVEADSIQINNYDISQKVTEHLINGGY
CCEEEEECCCCHHHHHHHHHHCCCEEEHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCC
KNTLLVAYKTQLQHLLQRTQAFLNTLEKHQIQNSQVLLADKDYIDRDINTGLENMLQNAV
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
KPDAIFFTSNKLAVSGLKKLTQLGINIPQDIAVAAFDETEAYELFPVPLTYVKQPLSAIG
CCCEEEEECCHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEECCCHHHHHHHHHHHH
TTAVKMLNEKINIKNLPLQNVTLHAELHIGQSTKSKHI
HHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKRISIKDVALKAGVSIASVSYVLNNKIDSRIPQETIDKVKHAAEELNYRPNNIAKSLKT
CCCCCHHHHHHHCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
QRTHIIGLVVADIANPYFSQLARIIEDEANKLGYTLLIGSSDENAEKFEALINMFADRQA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCC
DGLIIAPVENSEKCLLKLQKQKIPFVLIDRYLSGVEADSIQINNYDISQKVTEHLINGGY
CCEEEEECCCCHHHHHHHHHHCCCEEEHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCC
KNTLLVAYKTQLQHLLQRTQAFLNTLEKHQIQNSQVLLADKDYIDRDINTGLENMLQNAV
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
KPDAIFFTSNKLAVSGLKKLTQLGINIPQDIAVAAFDETEAYELFPVPLTYVKQPLSAIG
CCCEEEEECCHHHHHHHHHHHHHCCCCCCHHEEEEECCCCCEEEECCCHHHHHHHHHHHH
TTAVKMLNEKINIKNLPLQNVTLHAELHIGQSTKSKHI
HHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA