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The map label for this gene is ackA

Identifier: 146301270

GI number: 146301270

Start: 4207451

End: 4208635

Strand: Reverse

Name: ackA

Synonym: Fjoh_3528

Alternate gene names: 146301270

Gene position: 4208635-4207451 (Counterclockwise)

Preceding gene: 146301271

Following gene: 146301269

Centisome position: 69.03

GC content: 33.25

Gene sequence:

>1185_bases
ATGAAAATACTAATTATAAACTCAGGAAGTTCTTCAATTAAATATCAATTAATGGTTATGCCGGCAAATGAAGTAATTTG
TTCAGGCATGATTGACAGAATTGGTTTAGAAACATCGAATATTACTTTTAAAACAGTTTCAAATTCATTTGAAGAAATAC
TACCGGTTCCAACTCACAAAGTAGGATTGCAAAAAGTAGCCGATATGCTTCTGGATGCAGAAACGGGAGTAATTAAAACC
ACATCAGAAATTACCGCAGTAGGGCATAGAGTAGTTCACGGAGGAAGTTATTTTTCGAACACAACAGTTATTACCGAAGA
AGTAAAAGAAAAAATTAAAGAACTTTCAGAACTGGCTCCTCTTCACAATCCGGCGCATTTAGTTGGAATAAACGTTGCCG
AAGAAATTTTTGCTTCTGCAAAACAAGTAGCCGTTTTTGATACCGCTTTTCATCAGACAATTCCGGTTGAAGCTCATAAA
TATGCTATTCCTAATTTTCTTTTAACAGAACATAAAGTTCGAGTTTACGGCTTTCATGGAACAAGTCATAAATATGTTTC
AGAAAAGGCAATTAATTATTTAGAAAAAGGCTCTAAAATTATTACTATTCATTTAGGAAACGGCTGTAGTATGACAGCTG
TAAAAGACGGAAAAAGCATCGATACTACTATGGGGTTTTCACCAGCAAATGGTTTAGTTATGGGAACCCGTGCAGGAGAT
ATAGATCAGTCGGTTATTTTTTATATGGTAAAAAGTTTAGGCTATACACCAGATGAAGTCAATTCTATTTTATTAAAACA
AAGCGGAATGCTTGGTCTTACAGGATATAGCGATCTTCGTGATATTGAATCTAAAGCATCAGAAGGAAATAAAGACTGTC
AGCTGGCATTATTAATGAATGCTTACAGAATTAGAAAAACAATTGGTTCTTATGCCGCAGCTTTAAACGGATTAGATGCT
ATAGTTTTTACAGCTGGTATTGGAGAAAATTCTTCGTTTATGCGTAATTTAATCTGTACAGATATGGATTATTTTGGAAT
TGAAATCGACAAAGAAAAGAATCAAATTCGTTCTAAAGAATTACGAGAAATTAATACTCCAAATTCTATTGTAAAAATTC
TGGTAGTGCCAACAGACGAAGAATTTGAAATTGCAAATCAGGTTTATCAATTATTAGAGAATTAA

Upstream 100 bases:

>100_bases
CTTTTATTTCAATTTGAATTCGTTACATTAAAAATATAAAATAAAAAGTGCTGAGTCTTAAAAAATTAGAAACTTAGCAT
CTTAGAACCTTAAAAAAGAA

Downstream 100 bases:

>100_bases
TCTCTTAAAAATAAACTTTAACAATGGCAAAAATCATTGCAGTACTACTATTTTTTGTATTTCTTTTTCTTTCATTAATC
CATGTTTATTGGGCTTTTGG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKILIINSGSSSIKYQLMVMPANEVICSGMIDRIGLETSNITFKTVSNSFEEILPVPTHKVGLQKVADMLLDAETGVIKT
TSEITAVGHRVVHGGSYFSNTTVITEEVKEKIKELSELAPLHNPAHLVGINVAEEIFASAKQVAVFDTAFHQTIPVEAHK
YAIPNFLLTEHKVRVYGFHGTSHKYVSEKAINYLEKGSKIITIHLGNGCSMTAVKDGKSIDTTMGFSPANGLVMGTRAGD
IDQSVIFYMVKSLGYTPDEVNSILLKQSGMLGLTGYSDLRDIESKASEGNKDCQLALLMNAYRIRKTIGSYAAALNGLDA
IVFTAGIGENSSFMRNLICTDMDYFGIEIDKEKNQIRSKELREINTPNSIVKILVVPTDEEFEIANQVYQLLEN

Sequences:

>Translated_394_residues
MKILIINSGSSSIKYQLMVMPANEVICSGMIDRIGLETSNITFKTVSNSFEEILPVPTHKVGLQKVADMLLDAETGVIKT
TSEITAVGHRVVHGGSYFSNTTVITEEVKEKIKELSELAPLHNPAHLVGINVAEEIFASAKQVAVFDTAFHQTIPVEAHK
YAIPNFLLTEHKVRVYGFHGTSHKYVSEKAINYLEKGSKIITIHLGNGCSMTAVKDGKSIDTTMGFSPANGLVMGTRAGD
IDQSVIFYMVKSLGYTPDEVNSILLKQSGMLGLTGYSDLRDIESKASEGNKDCQLALLMNAYRIRKTIGSYAAALNGLDA
IVFTAGIGENSSFMRNLICTDMDYFGIEIDKEKNQIRSKELREINTPNSIVKILVVPTDEEFEIANQVYQLLEN
>Mature_394_residues
MKILIINSGSSSIKYQLMVMPANEVICSGMIDRIGLETSNITFKTVSNSFEEILPVPTHKVGLQKVADMLLDAETGVIKT
TSEITAVGHRVVHGGSYFSNTTVITEEVKEKIKELSELAPLHNPAHLVGINVAEEIFASAKQVAVFDTAFHQTIPVEAHK
YAIPNFLLTEHKVRVYGFHGTSHKYVSEKAINYLEKGSKIITIHLGNGCSMTAVKDGKSIDTTMGFSPANGLVMGTRAGD
IDQSVIFYMVKSLGYTPDEVNSILLKQSGMLGLTGYSDLRDIESKASEGNKDCQLALLMNAYRIRKTIGSYAAALNGLDA
IVFTAGIGENSSFMRNLICTDMDYFGIEIDKEKNQIRSKELREINTPNSIVKILVVPTDEEFEIANQVYQLLEN

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=41.2060301507538, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI145693184, Length=390, Percent_Identity=41.2820512820513, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_FLAJ1 (A5FE24)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001195861.1
- ProteinModelPortal:   A5FE24
- SMR:   A5FE24
- STRING:   A5FE24
- GeneID:   5091206
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_3528
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   IPYEYYQ
- ProtClustDB:   CLSK2537824
- BioCyc:   FJOH376686:FJOH_3528-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43237; Mature: 43237

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIINSGSSSIKYQLMVMPANEVICSGMIDRIGLETSNITFKTVSNSFEEILPVPTHK
CEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEHHCCHHHHCCCCCHH
VGLQKVADMLLDAETGVIKTTSEITAVGHRVVHGGSYFSNTTVITEEVKEKIKELSELAP
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHCC
LHNPAHLVGINVAEEIFASAKQVAVFDTAFHQTIPVEAHKYAIPNFLLTEHKVRVYGFHG
CCCCCEEEECCHHHHHHHCCHHEEEEEHHHHCCCCCCHHHEECCCEEEECCEEEEEEEEC
TSHKYVSEKAINYLEKGSKIITIHLGNGCSMTAVKDGKSIDTTMGFSPANGLVMGTRAGD
CCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCC
IDQSVIFYMVKSLGYTPDEVNSILLKQSGMLGLTGYSDLRDIESKASEGNKDCQLALLMN
CHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCEEEEHHHH
AYRIRKTIGSYAAALNGLDAIVFTAGIGENSSFMRNLICTDMDYFGIEIDKEKNQIRSKE
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEECHHHHHHHHH
LREINTPNSIVKILVVPTDEEFEIANQVYQLLEN
HHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILIINSGSSSIKYQLMVMPANEVICSGMIDRIGLETSNITFKTVSNSFEEILPVPTHK
CEEEEEECCCCCEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEEHHCCHHHHCCCCCHH
VGLQKVADMLLDAETGVIKTTSEITAVGHRVVHGGSYFSNTTVITEEVKEKIKELSELAP
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHCC
LHNPAHLVGINVAEEIFASAKQVAVFDTAFHQTIPVEAHKYAIPNFLLTEHKVRVYGFHG
CCCCCEEEECCHHHHHHHCCHHEEEEEHHHHCCCCCCHHHEECCCEEEECCEEEEEEEEC
TSHKYVSEKAINYLEKGSKIITIHLGNGCSMTAVKDGKSIDTTMGFSPANGLVMGTRAGD
CCCHHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCC
IDQSVIFYMVKSLGYTPDEVNSILLKQSGMLGLTGYSDLRDIESKASEGNKDCQLALLMN
CHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCEEEEHHHH
AYRIRKTIGSYAAALNGLDAIVFTAGIGENSSFMRNLICTDMDYFGIEIDKEKNQIRSKE
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCEEEECHHHHHHHHH
LREINTPNSIVKILVVPTDEEFEIANQVYQLLEN
HHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA