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The map label for this gene is actII-2 [H]

Identifier: 146301147

GI number: 146301147

Start: 4068930

End: 4070345

Strand: Reverse

Name: actII-2 [H]

Synonym: Fjoh_3405

Alternate gene names: 146301147

Gene position: 4070345-4068930 (Counterclockwise)

Preceding gene: 146301148

Following gene: 146301145

Centisome position: 66.76

GC content: 34.53

Gene sequence:

>1416_bases
ATGAAAAATAAAAAATACATTTCGCTCCTTATATTGCTGCTGGCACATTTGCTCACTATTTTGGATATCTTTATTGTAAA
TGTTGCTATTCCATCAATTCAGCGCGGGCTTGGTTCAAGTAATGGAAAAATTCAGTTAGTTGTAGCCATGTACATGGTTG
GATTTGCTTCTTTCCTTATAATTGGAGGTAAGACTGGAGATTATTTCGGCAGAAAGAAAATTTTTGTTTTAGGAATGCTC
TTATTTATGATTAGCTCTGCGGGCTGTGGTTTTGCAGATTCGACTGACCATCTTATTATTTTGAGGTTTTTTCAGGGAAT
AAGTGCAGGATTGATGTCTCCTCAGGTTTTGTCTTATATACAAGTTCTTTTTACAGATTACAAAGAGCGCACCTACGCAA
TGAGCTGGTATGGAATTGCGATTGGTATTGGCACTATGCTGGGACAGTTTCTGGGTGGTTTTTTGGTAGAACTCAAACCA
TATTTAATTGATCAATCCTGGCGGTATATATTTCTTATTAATATTCCTATTTGTGCTTCGGGGATTTTTCTTGCCATTCG
TTATCTTAATCCGTCCAGAGATTATTCTTCCCTGCAAATGGATTATTGGAGTGCTTTTATATTATGTATTGGACTCGCAT
TATTGGTCTTTTCCATGGCAGTTGGTTTAGAACAAAGCGGTTTGGTTAAAATTACCCTGCCAGTATCAATCGTATTTATT
TGTGTTTTTATATTCAGGCAGATAAAAAGGAAATCAAATAAAAAAGAACCTTTATTAAACCTTGAACTTTTTAAGAACAG
GAATTTCAATATGGCGGTTTCAGCATCAGCACTTTTTATGTTAATGCTTGATGCTTACTTTTTTGTGCTTGCTGTTTTTT
TACAAGAAGGAATTCAGCTTTCTCCCATGCAGGCAGGATATTTTATTGTATTTCAAGGAAGCGGGTTTATTCTGGCATCA
TTTTTATCAGCCAGATTAGTACCGCGGTTTGGTAAAACTGTATTGATTTTTGGTGTACTGCTTCTTATTTCAGCCATCAT
TTTACAGCTTGTTCTTTTTTATTATCCAATAGTTGATTTTAGAGGATATGTAATAATGTTTCTGCATGGTGTTGGTGTAG
CGCTGGTTTTACCTTCATTTGCTAATATAGCTTTAAAAAATCTGCCTCAAAATATGATTGGAAACGCATCAGGTGTTTAT
TCTACGGTACAGCAATTATTTGGTGCATTGGGCATTGCATTGACAGGAAGCGTATTCTACTATTTAGTAAAAGGAAATAC
AGATTTCAAGCATTTTTATAACGCTTTTTTCTATGGTACATTTATTCATTTGTTTTGCTTAATAAGCGTACTGCTGATTT
TATTTTTGCTGCCAAAGTCAGTTCTTCCAAAAAAAGAAAAGAATAGTGAAAAATAA

Upstream 100 bases:

>100_bases
TATAAATGGAAATACGAATGATTTAAAAGCCGTAAAAAAAAGTATAAATCAGTTTTTCTGATTTTTTTTAACCAAAAATA
GATAGACTGTTCTATATATT

Downstream 100 bases:

>100_bases
TTACGGTAATGATTAACTGACGATTACATCGTGTTTAAATAGCAGTCCTTTAACCTCATTCAAAGAAAAAACAGCCTTTT
TTAATTTCGTTTTAGAAGGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MKNKKYISLLILLLAHLLTILDIFIVNVAIPSIQRGLGSSNGKIQLVVAMYMVGFASFLIIGGKTGDYFGRKKIFVLGML
LFMISSAGCGFADSTDHLIILRFFQGISAGLMSPQVLSYIQVLFTDYKERTYAMSWYGIAIGIGTMLGQFLGGFLVELKP
YLIDQSWRYIFLINIPICASGIFLAIRYLNPSRDYSSLQMDYWSAFILCIGLALLVFSMAVGLEQSGLVKITLPVSIVFI
CVFIFRQIKRKSNKKEPLLNLELFKNRNFNMAVSASALFMLMLDAYFFVLAVFLQEGIQLSPMQAGYFIVFQGSGFILAS
FLSARLVPRFGKTVLIFGVLLLISAIILQLVLFYYPIVDFRGYVIMFLHGVGVALVLPSFANIALKNLPQNMIGNASGVY
STVQQLFGALGIALTGSVFYYLVKGNTDFKHFYNAFFYGTFIHLFCLISVLLILFLLPKSVLPKKEKNSEK

Sequences:

>Translated_471_residues
MKNKKYISLLILLLAHLLTILDIFIVNVAIPSIQRGLGSSNGKIQLVVAMYMVGFASFLIIGGKTGDYFGRKKIFVLGML
LFMISSAGCGFADSTDHLIILRFFQGISAGLMSPQVLSYIQVLFTDYKERTYAMSWYGIAIGIGTMLGQFLGGFLVELKP
YLIDQSWRYIFLINIPICASGIFLAIRYLNPSRDYSSLQMDYWSAFILCIGLALLVFSMAVGLEQSGLVKITLPVSIVFI
CVFIFRQIKRKSNKKEPLLNLELFKNRNFNMAVSASALFMLMLDAYFFVLAVFLQEGIQLSPMQAGYFIVFQGSGFILAS
FLSARLVPRFGKTVLIFGVLLLISAIILQLVLFYYPIVDFRGYVIMFLHGVGVALVLPSFANIALKNLPQNMIGNASGVY
STVQQLFGALGIALTGSVFYYLVKGNTDFKHFYNAFFYGTFIHLFCLISVLLILFLLPKSVLPKKEKNSEK
>Mature_471_residues
MKNKKYISLLILLLAHLLTILDIFIVNVAIPSIQRGLGSSNGKIQLVVAMYMVGFASFLIIGGKTGDYFGRKKIFVLGML
LFMISSAGCGFADSTDHLIILRFFQGISAGLMSPQVLSYIQVLFTDYKERTYAMSWYGIAIGIGTMLGQFLGGFLVELKP
YLIDQSWRYIFLINIPICASGIFLAIRYLNPSRDYSSLQMDYWSAFILCIGLALLVFSMAVGLEQSGLVKITLPVSIVFI
CVFIFRQIKRKSNKKEPLLNLELFKNRNFNMAVSASALFMLMLDAYFFVLAVFLQEGIQLSPMQAGYFIVFQGSGFILAS
FLSARLVPRFGKTVLIFGVLLLISAIILQLVLFYYPIVDFRGYVIMFLHGVGVALVLPSFANIALKNLPQNMIGNASGVY
STVQQLFGALGIALTGSVFYYLVKGNTDFKHFYNAFFYGTFIHLFCLISVLLILFLLPKSVLPKKEKNSEK

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=486, Percent_Identity=25.5144032921811, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=26.1845386533666, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788710, Length=421, Percent_Identity=25.8907363420428, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789042, Length=429, Percent_Identity=26.1072261072261, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=24.578313253012, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788509, Length=174, Percent_Identity=28.1609195402299, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=429, Percent_Identity=21.4452214452214, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52610; Mature: 52610

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKYISLLILLLAHLLTILDIFIVNVAIPSIQRGLGSSNGKIQLVVAMYMVGFASFLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHE
IGGKTGDYFGRKKIFVLGMLLFMISSAGCGFADSTDHLIILRFFQGISAGLMSPQVLSYI
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHH
QVLFTDYKERTYAMSWYGIAIGIGTMLGQFLGGFLVELKPYLIDQSWRYIFLINIPICAS
HHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHC
GIFLAIRYLNPSRDYSSLQMDYWSAFILCIGLALLVFSMAVGLEQSGLVKITLPVSIVFI
CHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
CVFIFRQIKRKSNKKEPLLNLELFKNRNFNMAVSASALFMLMLDAYFFVLAVFLQEGIQL
HHHHHHHHHHHCCCCCCCEEHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEE
SPMQAGYFIVFQGSGFILASFLSARLVPRFGKTVLIFGVLLLISAIILQLVLFYYPIVDF
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGYVIMFLHGVGVALVLPSFANIALKNLPQNMIGNASGVYSTVQQLFGALGIALTGSVFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
YLVKGNTDFKHFYNAFFYGTFIHLFCLISVLLILFLLPKSVLPKKEKNSEK
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCC
>Mature Secondary Structure
MKNKKYISLLILLLAHLLTILDIFIVNVAIPSIQRGLGSSNGKIQLVVAMYMVGFASFLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHE
IGGKTGDYFGRKKIFVLGMLLFMISSAGCGFADSTDHLIILRFFQGISAGLMSPQVLSYI
ECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCHHHHHHH
QVLFTDYKERTYAMSWYGIAIGIGTMLGQFLGGFLVELKPYLIDQSWRYIFLINIPICAS
HHHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHC
GIFLAIRYLNPSRDYSSLQMDYWSAFILCIGLALLVFSMAVGLEQSGLVKITLPVSIVFI
CHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHH
CVFIFRQIKRKSNKKEPLLNLELFKNRNFNMAVSASALFMLMLDAYFFVLAVFLQEGIQL
HHHHHHHHHHHCCCCCCCEEHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEE
SPMQAGYFIVFQGSGFILASFLSARLVPRFGKTVLIFGVLLLISAIILQLVLFYYPIVDF
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGYVIMFLHGVGVALVLPSFANIALKNLPQNMIGNASGVYSTVQQLFGALGIALTGSVFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
YLVKGNTDFKHFYNAFFYGTFIHLFCLISVLLILFLLPKSVLPKKEKNSEK
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]