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The map label for this gene is oprM [H]

Identifier: 146300984

GI number: 146300984

Start: 3861008

End: 3862462

Strand: Direct

Name: oprM [H]

Synonym: Fjoh_3240

Alternate gene names: 146300984

Gene position: 3861008-3862462 (Clockwise)

Preceding gene: 146300983

Following gene: 146300986

Centisome position: 63.33

GC content: 36.43

Gene sequence:

>1455_bases
ATGAAACAAGAAATAAATAGATATATAATCTTCGGAGGCGTTTTACTGCTTACAGCCTGCTCTGCTCCTAAAATCAACAA
TAATTTCGCGGCAAAACAACTGCCTGAAAATTTTGATTTGAAAAGAAAAAAAGCAGAAGACAGTCTGCAGGATTTTATTC
CGCTAAAAACAGAAACCTATTTTAAAGATGCCAAACTGGAAAACCTTTTAAAGAAAGCCATTGCCCAAAATCCTGACTAT
TTAATTATGCAGGAAAGAATTTTAATTGCCAATTCGCATTTAAAAGCAGCAAAACTGGGGCTTCTTCCTTCTTTGGATTT
TGTGGCTGATGCTTCCGGAACGCATTATGGAAAATACACTATGGACGGTGTAGGGAATTTTGATACCAATTTTTCGCAAA
ATATTAATGAAAAACAAAGGATAAACGAAGATGTAACCCCTAACCTCTTTTTAGGCGGAAAAGTTTCGTGGGAAGCTGAT
ATTTGGGGAAAACTGAGCAATCGAAAAAAAGCGGCTCGTGAACGTTTCTTTGCTTCTCAGGAAGGAATGCGTTTACTGCA
AACCCGATTGTTAAGCGATGTCGCCGATTTGTATTATAAACTGATCGCTTTAGACAAACAGGCTTCGATTTACGATAAAA
ACCTCAAAACACAACAAAAAGCGCTTGATATTATTTCGGCACAACGATCTGTGGGAAAAGCAACCGAATTGGCTGTACAG
CAGTTTAATGCTCAGAATAATAATATTTTAGCCGAAGCTGAACAGCTGAATTTAAGCATTGACCAAACTGAAAAAGCGTT
ATTAAATCTTTTAGGGGAATATGGCGGAAAAATCGACAGAAGTTCTGATTTTCTTTCTGGTCATCTTGAGGTGTTAAATC
AAAAAATAAGCGTAGATTCGATTATTCATAAAAGACCCGATGTATCTGAAGCGTATCATGAACTTCGTGCCTCAAATGCC
GATGCTAAGGCTGCACGTGCTGCCTTTTTCCCGACTTTAAACTTGGGAGGTTATGCCGGATTTAACTCGTTTTCTTTTTC
AACTTTTTTTGATAACGGATCGTTTGCCTGGCAATTGCTTGGCGGATTAACAGCTCCGGTTTTTAATAAGGGACAAATAA
AACAAGAATTTTTTGTATCTAATAAAAGACAGCAGATTTCGTTTCTGCAATATCAAAATACAATTACAACAGCGTATAAT
GAACTCAGTGCTTTACTGCACAGAACAGAATCTTTTGTTGATGTTTTGAAATATAAATCGAAGGAAATTGAACATCTCGA
AATTGCTGTAAACGTTTCAAATGATTTGTATTTGAGCGGTTATGCCAATTATCTGGAAATTATTAATGCCCAAAAAAATA
AACTGCAGGCCGAACTTGACTTTGTCGACATTCAATTAAAAAATGCAGAGTCACAGGTTTTATTGTACAAGGCTTTAGGA
GGAGGAATTAACTAA

Upstream 100 bases:

>100_bases
TGGAGGAATGCTTATAGGAACTGTTTTCGGACTTGTAATCATTCCGGGACTTTATATCCTGTTTGCCAAATTAGAAAAAG
CAATGAATAAAAATTAAAAC

Downstream 100 bases:

>100_bases
TCTTGAAGCTGCTACTGGTCGTAAACTTTTGAATTAGTACTTAAAAACTGCAGTTATAAATCCCACCCGTTTTAACGGAT
GGGATTTTTTTATTTTTAAG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKQEINRYIIFGGVLLLTACSAPKINNNFAAKQLPENFDLKRKKAEDSLQDFIPLKTETYFKDAKLENLLKKAIAQNPDY
LIMQERILIANSHLKAAKLGLLPSLDFVADASGTHYGKYTMDGVGNFDTNFSQNINEKQRINEDVTPNLFLGGKVSWEAD
IWGKLSNRKKAARERFFASQEGMRLLQTRLLSDVADLYYKLIALDKQASIYDKNLKTQQKALDIISAQRSVGKATELAVQ
QFNAQNNNILAEAEQLNLSIDQTEKALLNLLGEYGGKIDRSSDFLSGHLEVLNQKISVDSIIHKRPDVSEAYHELRASNA
DAKAARAAFFPTLNLGGYAGFNSFSFSTFFDNGSFAWQLLGGLTAPVFNKGQIKQEFFVSNKRQQISFLQYQNTITTAYN
ELSALLHRTESFVDVLKYKSKEIEHLEIAVNVSNDLYLSGYANYLEIINAQKNKLQAELDFVDIQLKNAESQVLLYKALG
GGIN

Sequences:

>Translated_484_residues
MKQEINRYIIFGGVLLLTACSAPKINNNFAAKQLPENFDLKRKKAEDSLQDFIPLKTETYFKDAKLENLLKKAIAQNPDY
LIMQERILIANSHLKAAKLGLLPSLDFVADASGTHYGKYTMDGVGNFDTNFSQNINEKQRINEDVTPNLFLGGKVSWEAD
IWGKLSNRKKAARERFFASQEGMRLLQTRLLSDVADLYYKLIALDKQASIYDKNLKTQQKALDIISAQRSVGKATELAVQ
QFNAQNNNILAEAEQLNLSIDQTEKALLNLLGEYGGKIDRSSDFLSGHLEVLNQKISVDSIIHKRPDVSEAYHELRASNA
DAKAARAAFFPTLNLGGYAGFNSFSFSTFFDNGSFAWQLLGGLTAPVFNKGQIKQEFFVSNKRQQISFLQYQNTITTAYN
ELSALLHRTESFVDVLKYKSKEIEHLEIAVNVSNDLYLSGYANYLEIINAQKNKLQAELDFVDIQLKNAESQVLLYKALG
GGIN
>Mature_484_residues
MKQEINRYIIFGGVLLLTACSAPKINNNFAAKQLPENFDLKRKKAEDSLQDFIPLKTETYFKDAKLENLLKKAIAQNPDY
LIMQERILIANSHLKAAKLGLLPSLDFVADASGTHYGKYTMDGVGNFDTNFSQNINEKQRINEDVTPNLFLGGKVSWEAD
IWGKLSNRKKAARERFFASQEGMRLLQTRLLSDVADLYYKLIALDKQASIYDKNLKTQQKALDIISAQRSVGKATELAVQ
QFNAQNNNILAEAEQLNLSIDQTEKALLNLLGEYGGKIDRSSDFLSGHLEVLNQKISVDSIIHKRPDVSEAYHELRASNA
DAKAARAAFFPTLNLGGYAGFNSFSFSTFFDNGSFAWQLLGGLTAPVFNKGQIKQEFFVSNKRQQISFLQYQNTITTAYN
ELSALLHRTESFVDVLKYKSKEIEHLEIAVNVSNDLYLSGYANYLEIINAQKNKLQAELDFVDIQLKNAESQVLLYKALG
GGIN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=25.5230125523013, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI2367348, Length=408, Percent_Identity=21.5686274509804, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54347; Mature: 54347

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEINRYIIFGGVLLLTACSAPKINNNFAAKQLPENFDLKRKKAEDSLQDFIPLKTETY
CCCCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHH
FKDAKLENLLKKAIAQNPDYLIMQERILIANSHLKAAKLGLLPSLDFVADASGTHYGKYT
HHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCHHHHHCCCCCCHHHCCCCCCCCCCEE
MDGVGNFDTNFSQNINEKQRINEDVTPNLFLGGKVSWEADIWGKLSNRKKAARERFFASQ
ECCCCCCCCCCCCCCCHHHHCCCCCCCCEEECCEEEECHHHHHHHHHHHHHHHHHHHHHH
EGMRLLQTRLLSDVADLYYKLIALDKQASIYDKNLKTQQKALDIISAQRSVGKATELAVQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QFNAQNNNILAEAEQLNLSIDQTEKALLNLLGEYGGKIDRSSDFLSGHLEVLNQKISVDS
HHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHH
IIHKRPDVSEAYHELRASNADAKAARAAFFPTLNLGGYAGFNSFSFSTFFDNGSFAWQLL
HHHCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
GGLTAPVFNKGQIKQEFFVSNKRQQISFLQYQNTITTAYNELSALLHRTESFVDVLKYKS
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KEIEHLEIAVNVSNDLYLSGYANYLEIINAQKNKLQAELDFVDIQLKNAESQVLLYKALG
CCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCEEEEEECC
GGIN
CCCC
>Mature Secondary Structure
MKQEINRYIIFGGVLLLTACSAPKINNNFAAKQLPENFDLKRKKAEDSLQDFIPLKTETY
CCCCCCEEEEEHHHHHHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHH
FKDAKLENLLKKAIAQNPDYLIMQERILIANSHLKAAKLGLLPSLDFVADASGTHYGKYT
HHHHHHHHHHHHHHHCCCCEEEEECEEEEECCCCHHHHHCCCCCCHHHCCCCCCCCCCEE
MDGVGNFDTNFSQNINEKQRINEDVTPNLFLGGKVSWEADIWGKLSNRKKAARERFFASQ
ECCCCCCCCCCCCCCCHHHHCCCCCCCCEEECCEEEECHHHHHHHHHHHHHHHHHHHHHH
EGMRLLQTRLLSDVADLYYKLIALDKQASIYDKNLKTQQKALDIISAQRSVGKATELAVQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QFNAQNNNILAEAEQLNLSIDQTEKALLNLLGEYGGKIDRSSDFLSGHLEVLNQKISVDS
HHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHH
IIHKRPDVSEAYHELRASNADAKAARAAFFPTLNLGGYAGFNSFSFSTFFDNGSFAWQLL
HHHCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHH
GGLTAPVFNKGQIKQEFFVSNKRQQISFLQYQNTITTAYNELSALLHRTESFVDVLKYKS
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KEIEHLEIAVNVSNDLYLSGYANYLEIINAQKNKLQAELDFVDIQLKNAESQVLLYKALG
CCCEEEEEEEEECCCEEECCHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCEEEEEECC
GGIN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]