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The map label for this gene is leuB [H]

Identifier: 146300613

GI number: 146300613

Start: 3398973

End: 3400091

Strand: Reverse

Name: leuB [H]

Synonym: Fjoh_2864

Alternate gene names: 146300613

Gene position: 3400091-3398973 (Counterclockwise)

Preceding gene: 146300614

Following gene: 146300612

Centisome position: 55.77

GC content: 34.32

Gene sequence:

>1119_bases
ATGAATTTGAAAATAGCAGTTTTACCAGGAGACGGAATTGGACCAGAAGTTATTGCACAGGCCAAAAAAGCATTATATGC
TATTGGCGAAGTGTACAATCATGAATTTGTTTTTGAAGAAGCACTCATGGGTGCGATCGCTATTGACAAAACTGGAAACC
CGCTCCCGGAGCAGACTTTAAATCTTTGTTTAAATACTGATGCTGTTTTGCTTGGTGCTATTGGAGATCCAAAATACGAT
AACAACCCAAATGCAAAAGTTCGTCCGGAGCAAGGTTTGTTAAAACTTCGAAAAGAACTTGGTTTGTTTGCCAATATTCG
TCCTATTAAACCATTTAAGGCTTTAATTGATGCTTCTCCTTTAAAAAGAGAGATTATTGAAGGAGCAGATTTTACAATTT
TCAGAGAATTAACCGGCGGTGCTTATTTTGGTACAAAAACATTAAATGATGAAGGCACACATGCTTCGGATTTATGTGAG
TATTCTGAAGAAGAAATAACGAGAATTGCACATTTAGCTTTTAAATCGGCACAAAACCGACGCAAGAAGTTAACTATGGT
AGACAAAGCAAATGTTTTGGAAACTTCCAGATTATGGAGAAAAGTGGTTCAAAAAGTAGGCGAAAGCTATCCAGATGTAA
TTTTGGACTTTTTATTTGTAGATAACGCAGCAATGCAGCTGATTTTGAATCCAAAACAATTTGATGTGATTTTAACTGAG
AATTTATTTGGAGATATTTTATCTGATGAAGCCAGCGTAATTACAGGATCTATTGGTTTACTGCCATCAATATCTTTAGG
AGAAAAAAATGCTTTGTTTGAGCCAATTCATGGTTCATATCCACAAGCAAAAGGAAAAAATATTGCCAATCCAATTGCTT
CGATTTTAGCTGCAGGACTTTTGTTAGAACATTTTGGATTAACTAAAGAAGCGAATGTAGTTTTTCAGGCAGTTAAAAAA
GCAATTGAATATGAAGTTGTTACTGTTGATTTAAAACCAGATTCAAAATTTGGTACAAATGAAGTAGGGGAGTTTGTTTC
CAATTTCATTTTCAGCAAAGATGACTTATTATATTTTAATAATGATAATGTTTCGATTGGTCAATCGACAATAGTTTAG

Upstream 100 bases:

>100_bases
CTAAAATTGAAGGTGAATTAATCAGAAGCTAATAAAACACAATAGAGCCAAGAAGAAAGAGAAAAAAATAAAGATTTAAA
TACATAAAGAACGAGACGTT

Downstream 100 bases:

>100_bases
ATTTTTTATTAAAATAATGAATTAAATAACAAGAAGTATTAGAAATGTTGAGATTTTATTTTTTTAGTTCTTAAAGTTTT
TGTACTTTTGTAACACTAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNLKIAVLPGDGIGPEVIAQAKKALYAIGEVYNHEFVFEEALMGAIAIDKTGNPLPEQTLNLCLNTDAVLLGAIGDPKYD
NNPNAKVRPEQGLLKLRKELGLFANIRPIKPFKALIDASPLKREIIEGADFTIFRELTGGAYFGTKTLNDEGTHASDLCE
YSEEEITRIAHLAFKSAQNRRKKLTMVDKANVLETSRLWRKVVQKVGESYPDVILDFLFVDNAAMQLILNPKQFDVILTE
NLFGDILSDEASVITGSIGLLPSISLGEKNALFEPIHGSYPQAKGKNIANPIASILAAGLLLEHFGLTKEANVVFQAVKK
AIEYEVVTVDLKPDSKFGTNEVGEFVSNFIFSKDDLLYFNNDNVSIGQSTIV

Sequences:

>Translated_372_residues
MNLKIAVLPGDGIGPEVIAQAKKALYAIGEVYNHEFVFEEALMGAIAIDKTGNPLPEQTLNLCLNTDAVLLGAIGDPKYD
NNPNAKVRPEQGLLKLRKELGLFANIRPIKPFKALIDASPLKREIIEGADFTIFRELTGGAYFGTKTLNDEGTHASDLCE
YSEEEITRIAHLAFKSAQNRRKKLTMVDKANVLETSRLWRKVVQKVGESYPDVILDFLFVDNAAMQLILNPKQFDVILTE
NLFGDILSDEASVITGSIGLLPSISLGEKNALFEPIHGSYPQAKGKNIANPIASILAAGLLLEHFGLTKEANVVFQAVKK
AIEYEVVTVDLKPDSKFGTNEVGEFVSNFIFSKDDLLYFNNDNVSIGQSTIV
>Mature_372_residues
MNLKIAVLPGDGIGPEVIAQAKKALYAIGEVYNHEFVFEEALMGAIAIDKTGNPLPEQTLNLCLNTDAVLLGAIGDPKYD
NNPNAKVRPEQGLLKLRKELGLFANIRPIKPFKALIDASPLKREIIEGADFTIFRELTGGAYFGTKTLNDEGTHASDLCE
YSEEEITRIAHLAFKSAQNRRKKLTMVDKANVLETSRLWRKVVQKVGESYPDVILDFLFVDNAAMQLILNPKQFDVILTE
NLFGDILSDEASVITGSIGLLPSISLGEKNALFEPIHGSYPQAKGKNIANPIASILAAGLLLEHFGLTKEANVVFQAVKK
AIEYEVVTVDLKPDSKFGTNEVGEFVSNFIFSKDDLLYFNNDNVSIGQSTIV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=336, Percent_Identity=31.25, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI28178821, Length=321, Percent_Identity=29.9065420560748, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178816, Length=321, Percent_Identity=29.9065420560748, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178838, Length=337, Percent_Identity=29.3768545994065, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI4758582, Length=329, Percent_Identity=29.483282674772, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI28178819, Length=153, Percent_Identity=36.6013071895425, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=46.218487394958, Blast_Score=291, Evalue=3e-80,
Organism=Escherichia coli, GI1788101, Length=364, Percent_Identity=32.4175824175824, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI1787381, Length=379, Percent_Identity=27.1767810026385, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17550882, Length=342, Percent_Identity=31.8713450292398, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17505779, Length=331, Percent_Identity=32.9305135951662, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71986051, Length=356, Percent_Identity=28.3707865168539, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=362, Percent_Identity=32.0441988950276, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6319830, Length=357, Percent_Identity=45.3781512605042, Blast_Score=265, Evalue=7e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=333, Percent_Identity=35.4354354354354, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=323, Percent_Identity=29.4117647058824, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=347, Percent_Identity=29.6829971181556, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24643270, Length=345, Percent_Identity=31.8840579710145, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24643268, Length=345, Percent_Identity=31.8840579710145, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI281362242, Length=341, Percent_Identity=30.4985337243402, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24648872, Length=341, Percent_Identity=30.4985337243402, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI161078633, Length=351, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24650122, Length=351, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078635, Length=351, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078637, Length=351, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078639, Length=349, Percent_Identity=29.2263610315186, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24661184, Length=338, Percent_Identity=28.4023668639053, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI20130355, Length=220, Percent_Identity=25.4545454545455, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 40798; Mature: 40798

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKIAVLPGDGIGPEVIAQAKKALYAIGEVYNHEFVFEEALMGAIAIDKTGNPLPEQTL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCHHHH
NLCLNTDAVLLGAIGDPKYDNNPNAKVRPEQGLLKLRKELGLFANIRPIKPFKALIDASP
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCH
LKREIIEGADFTIFRELTGGAYFGTKTLNDEGTHASDLCEYSEEEITRIAHLAFKSAQNR
HHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
RKKLTMVDKANVLETSRLWRKVVQKVGESYPDVILDFLFVDNAAMQLILNPKQFDVILTE
HHHEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHEEEEECCCCEEEEEEH
NLFGDILSDEASVITGSIGLLPSISLGEKNALFEPIHGSYPQAKGKNIANPIASILAAGL
HHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LLEHFGLTKEANVVFQAVKKAIEYEVVTVDLKPDSKFGTNEVGEFVSNFIFSKDDLLYFN
HHHHHCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEC
NDNVSIGQSTIV
CCCCCCCCCCCC
>Mature Secondary Structure
MNLKIAVLPGDGIGPEVIAQAKKALYAIGEVYNHEFVFEEALMGAIAIDKTGNPLPEQTL
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCHHHH
NLCLNTDAVLLGAIGDPKYDNNPNAKVRPEQGLLKLRKELGLFANIRPIKPFKALIDASP
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCH
LKREIIEGADFTIFRELTGGAYFGTKTLNDEGTHASDLCEYSEEEITRIAHLAFKSAQNR
HHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
RKKLTMVDKANVLETSRLWRKVVQKVGESYPDVILDFLFVDNAAMQLILNPKQFDVILTE
HHHEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHEEEEECCCCEEEEEEH
NLFGDILSDEASVITGSIGLLPSISLGEKNALFEPIHGSYPQAKGKNIANPIASILAAGL
HHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LLEHFGLTKEANVVFQAVKKAIEYEVVTVDLKPDSKFGTNEVGEFVSNFIFSKDDLLYFN
HHHHHCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEC
NDNVSIGQSTIV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA