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The map label for this gene is pbpE [H]

Identifier: 146300518

GI number: 146300518

Start: 3300776

End: 3302227

Strand: Reverse

Name: pbpE [H]

Synonym: Fjoh_2768

Alternate gene names: 146300518

Gene position: 3302227-3300776 (Counterclockwise)

Preceding gene: 146300519

Following gene: 146300517

Centisome position: 54.16

GC content: 29.48

Gene sequence:

>1452_bases
ATGAAAAAAACATTTAATGTTTTTTTGTTTCTGTTTGTTATAAATTTCAGCTTCGGCCAGAACACAAATAACCAATTAAC
AGAACAAAAAATAGACAGTTACATAAAAGAAGTAATTGAAGTAAATGAAATTCCCGGTACTGCACTCGCCGTAATAAAAA
ACGGAAAAGTCATTTATGAAAAGTATTTTGGAAAAGCTTCTCTGGAAGAAAATAAAGCAGTGGATAAAAACACTGCTTTT
AAAATTTTCTCTACAACCAAACTAATCACAAATGTTGGTGTATTTCAGCTTATAGACAAAGGAAAACTTTCTTTAGAAGA
TCCTATTACAAAATATTTAGAAAATCTTCCTAAAGAATGGCAGGATATAAAAATTAAAAATCTGCTGACGCATTCTTCGG
GCCTGCCTGATTTCGTTAAGTTTGATGATATTTCTATTTCAATTCCCTATTCTGAAAAACTTGCTATTTTATCAAAAAAG
CCAATGGAGTTTGCAACCGGAAATGAATATCGCTACAATCAGACTAACTATTTGTTTCTTGCTAAAATTATTGAAAAAAT
TACGGGACAGACTTTTGAAGATTATATCTTACAAAATCAATTTCCATCAATACAATCTGGTGTTTATTTTTCATCAAACT
TTGGCGAAGCACATCCTAACAGCGCTTTTAGGTATGTTAACTATAATTATGAAACGAAAAAATATACTAAGAGTACAACA
AACAGCGGTTTTGATGCTCACTCGTCTAATGGGCTGAATATTACATTGCAGAATTTTATAAAATGGAATAAAAATCTGGA
TAATGATATTTTTCTTAAAAAGGAAACCAAATATTCTATGTGGAAACCTTTTTCTTATGGTAATCAAACAGATCGTTTTG
GATACGGATGGGAAATTACTCCTTTGAACAAAATTTTATCTTACGGATTCAGCGGCGGTAATGAAACAGCTTTTAGAAAA
TTTGTTAAGAATGATTTGACTATCATATTTTTATCTAACGGGCACAAATATTCTAATTTATACATTCACAGTCAGGTAAT
TAATCATGTAGCTTCAATCGCAGATCCAGGTCTTACTGATGAATATTTATTAGCCGATGAAAAAATAACTCAGGATTTTT
TAAAACTTGATATTGATAAATCAACTCAAAACTATTTCAATTTGAAAAAAGTTCATCCTGAATGGGATTTTGAATTTCGC
TTAAATGCTATTGGTTATACTTTAATGAATTACGGCAGAATTAATGACGCGATAAAAGTTTTTGAAATAAATACAAAAGA
AAATCCAAAATCAGGAAATGCATTTGATAGTCTGGCTGAAGGATTATTTAATAACAATCAGTTTGAAATTTCGAAAGAAA
ACTATAAAAAAGCATTAGAATTAAATCCAGACAATACTAACGCCAAAGAAATGTTGGATAAAATCAAAAAGATTACAGAA
AAGAAATCGTAA

Upstream 100 bases:

>100_bases
ATGAAGCAGCATGCCAAAGACATGAAACAGCATGAGAAGGATATGAAGGAATGGGAAAAAGAAAACAGAAAATCTTAATT
TTTACTTCTAAACCTCATAA

Downstream 100 bases:

>100_bases
AAGAGTTCTTCCTTAAACCACAAAAAAGACTTTGCCAGCTGCAAAGTCTTTTTTTATATCTTTGAAAAAACAAATCTGCT
ATGTATAAATTTTTAGCCAA

Product: beta-lactamase

Products: NA

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKKTFNVFLFLFVINFSFGQNTNNQLTEQKIDSYIKEVIEVNEIPGTALAVIKNGKVIYEKYFGKASLEENKAVDKNTAF
KIFSTTKLITNVGVFQLIDKGKLSLEDPITKYLENLPKEWQDIKIKNLLTHSSGLPDFVKFDDISISIPYSEKLAILSKK
PMEFATGNEYRYNQTNYLFLAKIIEKITGQTFEDYILQNQFPSIQSGVYFSSNFGEAHPNSAFRYVNYNYETKKYTKSTT
NSGFDAHSSNGLNITLQNFIKWNKNLDNDIFLKKETKYSMWKPFSYGNQTDRFGYGWEITPLNKILSYGFSGGNETAFRK
FVKNDLTIIFLSNGHKYSNLYIHSQVINHVASIADPGLTDEYLLADEKITQDFLKLDIDKSTQNYFNLKKVHPEWDFEFR
LNAIGYTLMNYGRINDAIKVFEINTKENPKSGNAFDSLAEGLFNNNQFEISKENYKKALELNPDNTNAKEMLDKIKKITE
KKS

Sequences:

>Translated_483_residues
MKKTFNVFLFLFVINFSFGQNTNNQLTEQKIDSYIKEVIEVNEIPGTALAVIKNGKVIYEKYFGKASLEENKAVDKNTAF
KIFSTTKLITNVGVFQLIDKGKLSLEDPITKYLENLPKEWQDIKIKNLLTHSSGLPDFVKFDDISISIPYSEKLAILSKK
PMEFATGNEYRYNQTNYLFLAKIIEKITGQTFEDYILQNQFPSIQSGVYFSSNFGEAHPNSAFRYVNYNYETKKYTKSTT
NSGFDAHSSNGLNITLQNFIKWNKNLDNDIFLKKETKYSMWKPFSYGNQTDRFGYGWEITPLNKILSYGFSGGNETAFRK
FVKNDLTIIFLSNGHKYSNLYIHSQVINHVASIADPGLTDEYLLADEKITQDFLKLDIDKSTQNYFNLKKVHPEWDFEFR
LNAIGYTLMNYGRINDAIKVFEINTKENPKSGNAFDSLAEGLFNNNQFEISKENYKKALELNPDNTNAKEMLDKIKKITE
KKS
>Mature_483_residues
MKKTFNVFLFLFVINFSFGQNTNNQLTEQKIDSYIKEVIEVNEIPGTALAVIKNGKVIYEKYFGKASLEENKAVDKNTAF
KIFSTTKLITNVGVFQLIDKGKLSLEDPITKYLENLPKEWQDIKIKNLLTHSSGLPDFVKFDDISISIPYSEKLAILSKK
PMEFATGNEYRYNQTNYLFLAKIIEKITGQTFEDYILQNQFPSIQSGVYFSSNFGEAHPNSAFRYVNYNYETKKYTKSTT
NSGFDAHSSNGLNITLQNFIKWNKNLDNDIFLKKETKYSMWKPFSYGNQTDRFGYGWEITPLNKILSYGFSGGNETAFRK
FVKNDLTIIFLSNGHKYSNLYIHSQVINHVASIADPGLTDEYLLADEKITQDFLKLDIDKSTQNYFNLKKVHPEWDFEFR
LNAIGYTLMNYGRINDAIKVFEINTKENPKSGNAFDSLAEGLFNNNQFEISKENYKKALELNPDNTNAKEMLDKIKKITE
KKS

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=416, Percent_Identity=22.1153846153846, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790593, Length=199, Percent_Identity=28.643216080402, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71983659, Length=375, Percent_Identity=26.4, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983666, Length=199, Percent_Identity=32.1608040201005, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17550934, Length=220, Percent_Identity=24.5454545454545, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115533026, Length=210, Percent_Identity=24.7619047619048, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: NA

Molecular weight: Translated: 55703; Mature: 55703

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50005 TPR L=RR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTFNVFLFLFVINFSFGQNTNNQLTEQKIDSYIKEVIEVNEIPGTALAVIKNGKVIYE
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEE
KYFGKASLEENKAVDKNTAFKIFSTTKLITNVGVFQLIDKGKLSLEDPITKYLENLPKEW
ECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCHHH
QDIKIKNLLTHSSGLPDFVKFDDISISIPYSEKLAILSKKPMEFATGNEYRYNQTNYLFL
HCEEHHHHHHCCCCCCCCEEECCEEEECCCCCCEEEECCCCCCCCCCCCEEECCCCHHHH
AKIIEKITGQTFEDYILQNQFPSIQSGVYFSSNFGEAHPNSAFRYVNYNYETKKYTKSTT
HHHHHHHCCCCHHHHHHHCCCCCHHCCEEEECCCCCCCCCCEEEEEECCCCCHHHCCCCC
NSGFDAHSSNGLNITLQNFIKWNKNLDNDIFLKKETKYSMWKPFSYGNQTDRFGYGWEIT
CCCCCCCCCCCCEEEHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEC
PLNKILSYGFSGGNETAFRKFVKNDLTIIFLSNGHKYSNLYIHSQVINHVASIADPGLTD
HHHHHHHCCCCCCCHHHHHHHHHCCEEEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCC
EYLLADEKITQDFLKLDIDKSTQNYFNLKKVHPEWDFEFRLNAIGYTLMNYGRINDAIKV
HHEECCHHHHHHHHHEECCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHCCCCCCCEEE
FEINTKENPKSGNAFDSLAEGLFNNNQFEISKENYKKALELNPDNTNAKEMLDKIKKITE
EEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHCEEEEECCCCCCHHHHHHHHHHHHH
KKS
CCC
>Mature Secondary Structure
MKKTFNVFLFLFVINFSFGQNTNNQLTEQKIDSYIKEVIEVNEIPGTALAVIKNGKVIYE
CCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEE
KYFGKASLEENKAVDKNTAFKIFSTTKLITNVGVFQLIDKGKLSLEDPITKYLENLPKEW
ECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHCCHHH
QDIKIKNLLTHSSGLPDFVKFDDISISIPYSEKLAILSKKPMEFATGNEYRYNQTNYLFL
HCEEHHHHHHCCCCCCCCEEECCEEEECCCCCCEEEECCCCCCCCCCCCEEECCCCHHHH
AKIIEKITGQTFEDYILQNQFPSIQSGVYFSSNFGEAHPNSAFRYVNYNYETKKYTKSTT
HHHHHHHCCCCHHHHHHHCCCCCHHCCEEEECCCCCCCCCCEEEEEECCCCCHHHCCCCC
NSGFDAHSSNGLNITLQNFIKWNKNLDNDIFLKKETKYSMWKPFSYGNQTDRFGYGWEIT
CCCCCCCCCCCCEEEHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEC
PLNKILSYGFSGGNETAFRKFVKNDLTIIFLSNGHKYSNLYIHSQVINHVASIADPGLTD
HHHHHHHCCCCCCCHHHHHHHHHCCEEEEEEECCCEEEEEEEHHHHHHHHHHHCCCCCCC
EYLLADEKITQDFLKLDIDKSTQNYFNLKKVHPEWDFEFRLNAIGYTLMNYGRINDAIKV
HHEECCHHHHHHHHHEECCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHCCCCCCCEEE
FEINTKENPKSGNAFDSLAEGLFNNNQFEISKENYKKALELNPDNTNAKEMLDKIKKITE
EEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHCEEEEECCCCCCHHHHHHHHHHHHH
KKS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]