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The map label for this gene is rlmL [H]

Identifier: 146300250

GI number: 146300250

Start: 2983161

End: 2984324

Strand: Direct

Name: rlmL [H]

Synonym: Fjoh_2495

Alternate gene names: 146300250

Gene position: 2983161-2984324 (Clockwise)

Preceding gene: 146300247

Following gene: 146300251

Centisome position: 48.93

GC content: 35.22

Gene sequence:

>1164_bases
ATGGAAGAAAATTTTAGAATGATAGCCAAATGTTTTTTTGGCTTTGAGGAAATATTAGAGAAAGAATTGCGCACGCTTGG
GGCTCAGGATGTTGAAAAAGGAGTGCGTATGGTAAGTTTTAAAGGAGATAAAGGTTTTATGTACAAAGCCAATTTGTCGC
TTAGAACTGCATTAAAAATCCTGAAACCAATTTATTCTTTCAGAGCTAACAATGAACAGGCTTTGTATAAGGGAATTTCG
GGCATTAACTGGTCGAAGTTGTTGAACGCTAATCAGACTTTTGTAATTGATTCAACAGTTCATTCTACTTATTTTAATCA
TTCTGAGTTTGTTTCTCAAAAATGTAAAGATGCTATTGTTGACCAGTTTAGAGAAAGAACGGGACAACGACCAAGTATTG
ATAAACAATATCCGGATTTAAGAATTAATGTTCATATTGATAAAGATCAGGTTTCGGTTTCTTTAGATACTTCGGGAAGT
TCACTTCATCAGCGCGGTTACAGAACGGCAACTAATATTGCGCCTATAAACGAGGTTTTAGCAGCTGGAATTCTTTTATT
ATCAGGCTGGGACGGACAAACTCACTTTTTAGATCCAATGTGCGGATCTGGAACTTTTCTTGCCGAAGCAGCTATGATTG
CCTGCAATATTCCGGCAAATATTAATCGTAAAGAATTTGCTTTTGAGAAATGGAAAGACTGGGACAACGATTTGTTTGAT
ACTATTGTTGACAGTTTGATGAAGAAAACAAGAGAGTTTCATTACACCATAAAAGGTTTTGATAAAGCGCCAAGTGCTGT
AAGCAAAGCGAAAGACAATATTAGAAATGCGAATCTTGAAGATTATATAACAATTCATGAAGATAACTTTTTTGATACTG
AAAAAGAAGTAGAAGGAAAACTGCATATTGTTTTTAATCCGCCGTATGATGAGCGTCTGGATATTCACATGGAGCAGTTC
TACAAAAATATCGGCGATACTTTAAAGAAAAATTATCCGGGAACAAATGCGTGGTTTATAACTGCAAACCTTGAAGCTTT
AAAATTCGTTGGATTAAAACCGTCAAGAAAAATTAAACTTTTTAACGGAAGTCTTGAAGCACGTTTGGTAAAATACGAAA
TGTACGAAGGAAGTAAGAGAACGAAGTTTCAGGTTTCTGAATAG

Upstream 100 bases:

>100_bases
TTAATTCAAAGTCCGGCAAATTTAGTGTATTTTTGCCGAAAAATAACTATTTCGCGGCATCTGTCTGCGATTGGGGTTGT
TTTTAATACAAAAAGTAAAA

Downstream 100 bases:

>100_bases
TTACAAAGTAACAAAGGTTCAAAGTAACAAAGGTTTTAAAACTTTGCCCGCTTTGAATCTCAAAAAAATGAAATAATAAG
TCAAGTAAATAATATAAAGC

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEENFRMIAKCFFGFEEILEKELRTLGAQDVEKGVRMVSFKGDKGFMYKANLSLRTALKILKPIYSFRANNEQALYKGIS
GINWSKLLNANQTFVIDSTVHSTYFNHSEFVSQKCKDAIVDQFRERTGQRPSIDKQYPDLRINVHIDKDQVSVSLDTSGS
SLHQRGYRTATNIAPINEVLAAGILLLSGWDGQTHFLDPMCGSGTFLAEAAMIACNIPANINRKEFAFEKWKDWDNDLFD
TIVDSLMKKTREFHYTIKGFDKAPSAVSKAKDNIRNANLEDYITIHEDNFFDTEKEVEGKLHIVFNPPYDERLDIHMEQF
YKNIGDTLKKNYPGTNAWFITANLEALKFVGLKPSRKIKLFNGSLEARLVKYEMYEGSKRTKFQVSE

Sequences:

>Translated_387_residues
MEENFRMIAKCFFGFEEILEKELRTLGAQDVEKGVRMVSFKGDKGFMYKANLSLRTALKILKPIYSFRANNEQALYKGIS
GINWSKLLNANQTFVIDSTVHSTYFNHSEFVSQKCKDAIVDQFRERTGQRPSIDKQYPDLRINVHIDKDQVSVSLDTSGS
SLHQRGYRTATNIAPINEVLAAGILLLSGWDGQTHFLDPMCGSGTFLAEAAMIACNIPANINRKEFAFEKWKDWDNDLFD
TIVDSLMKKTREFHYTIKGFDKAPSAVSKAKDNIRNANLEDYITIHEDNFFDTEKEVEGKLHIVFNPPYDERLDIHMEQF
YKNIGDTLKKNYPGTNAWFITANLEALKFVGLKPSRKIKLFNGSLEARLVKYEMYEGSKRTKFQVSE
>Mature_387_residues
MEENFRMIAKCFFGFEEILEKELRTLGAQDVEKGVRMVSFKGDKGFMYKANLSLRTALKILKPIYSFRANNEQALYKGIS
GINWSKLLNANQTFVIDSTVHSTYFNHSEFVSQKCKDAIVDQFRERTGQRPSIDKQYPDLRINVHIDKDQVSVSLDTSGS
SLHQRGYRTATNIAPINEVLAAGILLLSGWDGQTHFLDPMCGSGTFLAEAAMIACNIPANINRKEFAFEKWKDWDNDLFD
TIVDSLMKKTREFHYTIKGFDKAPSAVSKAKDNIRNANLEDYITIHEDNFFDTEKEVEGKLHIVFNPPYDERLDIHMEQF
YKNIGDTLKKNYPGTNAWFITANLEALKFVGLKPSRKIKLFNGSLEARLVKYEMYEGSKRTKFQVSE

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=363, Percent_Identity=32.5068870523416, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 44407; Mature: 44407

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENFRMIAKCFFGFEEILEKELRTLGAQDVEKGVRMVSFKGDKGFMYKANLSLRTALKI
CCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHH
LKPIYSFRANNEQALYKGISGINWSKLLNANQTFVIDSTVHSTYFNHSEFVSQKCKDAIV
HHHHHHHCCCCHHHHHHCCCCCCHHHHCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHH
DQFRERTGQRPSIDKQYPDLRINVHIDKDQVSVSLDTSGSSLHQRGYRTATNIAPINEVL
HHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCHHHHCCCCCCCCCCHHHHHH
AAGILLLSGWDGQTHFLDPMCGSGTFLAEAAMIACNIPANINRKEFAFEKWKDWDNDLFD
HHHEEEEECCCCCCEECCCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
TIVDSLMKKTREFHYTIKGFDKAPSAVSKAKDNIRNANLEDYITIHEDNFFDTEKEVEGK
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHCCCE
LHIVFNPPYDERLDIHMEQFYKNIGDTLKKNYPGTNAWFITANLEALKFVGLKPSRKIKL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHEEEEECCCCCCEEEE
FNGSLEARLVKYEMYEGSKRTKFQVSE
ECCCCEEEEEEEEEECCCCCCEEEECC
>Mature Secondary Structure
MEENFRMIAKCFFGFEEILEKELRTLGAQDVEKGVRMVSFKGDKGFMYKANLSLRTALKI
CCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHCCCEEEEEECCCCEEEEECCHHHHHHHH
LKPIYSFRANNEQALYKGISGINWSKLLNANQTFVIDSTVHSTYFNHSEFVSQKCKDAIV
HHHHHHHCCCCHHHHHHCCCCCCHHHHCCCCCEEEEECHHHHHHCCHHHHHHHHHHHHHH
DQFRERTGQRPSIDKQYPDLRINVHIDKDQVSVSLDTSGSSLHQRGYRTATNIAPINEVL
HHHHHHCCCCCCCCCCCCCEEEEEEEECCEEEEEEECCCCHHHHCCCCCCCCCCHHHHHH
AAGILLLSGWDGQTHFLDPMCGSGTFLAEAAMIACNIPANINRKEFAFEKWKDWDNDLFD
HHHEEEEECCCCCCEECCCCCCCCCHHHHHEEEEECCCCCCCHHHHHHHHHCCCCHHHHH
TIVDSLMKKTREFHYTIKGFDKAPSAVSKAKDNIRNANLEDYITIHEDNFFDTEKEVEGK
HHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHCCCE
LHIVFNPPYDERLDIHMEQFYKNIGDTLKKNYPGTNAWFITANLEALKFVGLKPSRKIKL
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHEEEEECCCCCCEEEE
FNGSLEARLVKYEMYEGSKRTKFQVSE
ECCCCEEEEEEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA