Click here to switch to the map view.

The map label for this gene is aprN [H]

Identifier: 146300144

GI number: 146300144

Start: 2864273

End: 2865874

Strand: Direct

Name: aprN [H]

Synonym: Fjoh_2389

Alternate gene names: 146300144

Gene position: 2864273-2865874 (Clockwise)

Preceding gene: 146300143

Following gene: 146300145

Centisome position: 46.98

GC content: 36.2

Gene sequence:

>1602_bases
ATGAGATATAACTTTACTTTTCTTTTATTCTTCGTTTCGTTTGCCATTTTTGCGCAGGAAGAAGCGTGGGTTTATTTTAA
TGCAAAACCCAATGCTCAGGCATTCTTTAGTAATCCTTTATCAGAACTATCGCAAAGAGCTTTAGACCGAAGAACAAATC
AAAATATAGTTTTAGATTTAACAGATGCACCGTTAGAAGCTTCATTTGTAAATCAAATTAAATCTAGTACAGGAATAACG
TTGATGGCACAGTCTAAATGGCTTAATGCACTTCACATTCGCGGTACACAAACTAATATCAATGCTTTAAAAGGATTGTC
TTTTGTAGCAAAAGTTGAGTTTGCTGATAAAAGTTTAAATACTACTGGAAAAAAAGTCTCAGTAGCAAAAACAAGTCAAA
CACTTGATAAGTTAAAAACTACTGTTGATTATTCGTATGGTAATTCTGCCAATCAAATTCAGATGTTAAACGGTCAGGTT
CTGCATCAGCAGAATTTTACCGGAGAAGGAAAAATTATAGCCGTTTTAGATGCTGGATTTCCAGGTGTAAATACAGCCCA
GCCTTTTGAAAATCTTAGAAATAACAACCGAATTCTGGGAGGTTACGACTTCACCACCCGAAATGACAATTTTTACACGG
GAGACGATCACGGAACTTTAGTACTTTCAACTATGGGAGGTTATAAAGAAAATGCCTTGGTTGGAACAGCTCCAAATGCA
TCATATTATCTTTTTATTACCGAAATCGATGCTGTAGAAAATCCTGTAGAAGAATCTTTATGGGTTGAAGCTGCCGAAAG
AGCTGATGCTTTGGGAGTTGATATAATAACTACTTCATTAGGATATTTTGGTGACCGCACAGAGCCGAGATATAATCATA
CCTACAGCGATATGAACGGAATAACAACTTTTATTTCGCGAGGCGCTGAAATAGCTTTTAGTAAAGGAATATTGGTTTTA
GCTTCTGCCGGAAATGAAGGAAATACGACAGAAAAACATGTTGGTTCTCCAGCCGATGCTGTTTCAGTTCTGGCAATTGG
TGCGGTTACAGCTTCTAAAGTAAAATCGAGTTTCAGTTCGATTGGGCCAAGTTATGATAACAGAATAAAGCCGGATGTAA
TGGCACAGGGATCTGCGGCGACAGTAGCTAATGCAAACGGAAGTATAGGAACTGCCGATGGAACTTCTTTTTCATGTCCA
ATTATAGCAGGAATGGCAGCTTCTTTATGGCAGGCTTTTCCAACTAAAACCAATAAAGAAATCAGACAAATGATTTTGGC
TTCTGCCGACAGATATACTACTCCTGATACTAGTTATGGATATGGAATACCAAATTTTGGTTCTACTTTAGGAACTCAAA
CTTTCCTGTCAACTTCAGATTTTTTGGTTTATCCAAATCCAACAAAATCAAATATCTCATTTTTGTTTGATAACGAAACC
GCTTCTGTTTCTATCTATTCGTTATTAGGACAAAAGCTGATTGAAAAACAAATCACAAATCAAAATCCGGTACTTTCTGT
AGAAGGTTTAACAAACGGACTTTATTTTTATACTTTCGATGCGGGAAGCTTACATAAAACAGGAAAAATAATAAAACAAT
AA

Upstream 100 bases:

>100_bases
GACAATTTGTTGCCTGGTATTTAGAAAATGAATTAGTTGGTTCGGGAGTAATTTCTTAGCTTTATACTTTCTTAGGAAGA
AATTCCATAAAAAGTATACT

Downstream 100 bases:

>100_bases
TAGTTTTAATGAATAGAATTACAACGCTTTTTAATATAAAATATCCAATTATTCAAGGTGGCATGATTTGGAATAGCGGT
TATAAATTAGCATCGGCAGT

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MRYNFTFLLFFVSFAIFAQEEAWVYFNAKPNAQAFFSNPLSELSQRALDRRTNQNIVLDLTDAPLEASFVNQIKSSTGIT
LMAQSKWLNALHIRGTQTNINALKGLSFVAKVEFADKSLNTTGKKVSVAKTSQTLDKLKTTVDYSYGNSANQIQMLNGQV
LHQQNFTGEGKIIAVLDAGFPGVNTAQPFENLRNNNRILGGYDFTTRNDNFYTGDDHGTLVLSTMGGYKENALVGTAPNA
SYYLFITEIDAVENPVEESLWVEAAERADALGVDIITTSLGYFGDRTEPRYNHTYSDMNGITTFISRGAEIAFSKGILVL
ASAGNEGNTTEKHVGSPADAVSVLAIGAVTASKVKSSFSSIGPSYDNRIKPDVMAQGSAATVANANGSIGTADGTSFSCP
IIAGMAASLWQAFPTKTNKEIRQMILASADRYTTPDTSYGYGIPNFGSTLGTQTFLSTSDFLVYPNPTKSNISFLFDNET
ASVSIYSLLGQKLIEKQITNQNPVLSVEGLTNGLYFYTFDAGSLHKTGKIIKQ

Sequences:

>Translated_533_residues
MRYNFTFLLFFVSFAIFAQEEAWVYFNAKPNAQAFFSNPLSELSQRALDRRTNQNIVLDLTDAPLEASFVNQIKSSTGIT
LMAQSKWLNALHIRGTQTNINALKGLSFVAKVEFADKSLNTTGKKVSVAKTSQTLDKLKTTVDYSYGNSANQIQMLNGQV
LHQQNFTGEGKIIAVLDAGFPGVNTAQPFENLRNNNRILGGYDFTTRNDNFYTGDDHGTLVLSTMGGYKENALVGTAPNA
SYYLFITEIDAVENPVEESLWVEAAERADALGVDIITTSLGYFGDRTEPRYNHTYSDMNGITTFISRGAEIAFSKGILVL
ASAGNEGNTTEKHVGSPADAVSVLAIGAVTASKVKSSFSSIGPSYDNRIKPDVMAQGSAATVANANGSIGTADGTSFSCP
IIAGMAASLWQAFPTKTNKEIRQMILASADRYTTPDTSYGYGIPNFGSTLGTQTFLSTSDFLVYPNPTKSNISFLFDNET
ASVSIYSLLGQKLIEKQITNQNPVLSVEGLTNGLYFYTFDAGSLHKTGKIIKQ
>Mature_533_residues
MRYNFTFLLFFVSFAIFAQEEAWVYFNAKPNAQAFFSNPLSELSQRALDRRTNQNIVLDLTDAPLEASFVNQIKSSTGIT
LMAQSKWLNALHIRGTQTNINALKGLSFVAKVEFADKSLNTTGKKVSVAKTSQTLDKLKTTVDYSYGNSANQIQMLNGQV
LHQQNFTGEGKIIAVLDAGFPGVNTAQPFENLRNNNRILGGYDFTTRNDNFYTGDDHGTLVLSTMGGYKENALVGTAPNA
SYYLFITEIDAVENPVEESLWVEAAERADALGVDIITTSLGYFGDRTEPRYNHTYSDMNGITTFISRGAEIAFSKGILVL
ASAGNEGNTTEKHVGSPADAVSVLAIGAVTASKVKSSFSSIGPSYDNRIKPDVMAQGSAATVANANGSIGTADGTSFSCP
IIAGMAASLWQAFPTKTNKEIRQMILASADRYTTPDTSYGYGIPNFGSTLGTQTFLSTSDFLVYPNPTKSNISFLFDNET
ASVSIYSLLGQKLIEKQITNQNPVLSVEGLTNGLYFYTFDAGSLHKTGKIIKQ

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 57773; Mature: 57773

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00136 SUBTILASE_ASP ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYNFTFLLFFVSFAIFAQEEAWVYFNAKPNAQAFFSNPLSELSQRALDRRTNQNIVLDL
CCEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEE
TDAPLEASFVNQIKSSTGITLMAQSKWLNALHIRGTQTNINALKGLSFVAKVEFADKSLN
CCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHHCCEEEEEEEECCCCCC
TTGKKVSVAKTSQTLDKLKTTVDYSYGNSANQIQMLNGQVLHQQNFTGEGKIIAVLDAGF
CCCCEEEEEHHHHHHHHHHHHEEECCCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCC
PGVNTAQPFENLRNNNRILGGYDFTTRNDNFYTGDDHGTLVLSTMGGYKENALVGTAPNA
CCCCCCHHHHHHHCCCEEEECEEEEECCCCEEECCCCCEEEEEECCCCCCCCEEECCCCC
SYYLFITEIDAVENPVEESLWVEAAERADALGVDIITTSLGYFGDRTEPRYNHTYSDMNG
EEEEEEEEECHHCCCHHHHHHHHHHHHCCCCCEEHEEHHHHCCCCCCCCCCCCCHHHHHH
ITTFISRGAEIAFSKGILVLASAGNEGNTTEKHVGSPADAVSVLAIGAVTASKVKSSFSS
HHHHHHCCCCEEEECCEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGPSYDNRIKPDVMAQGSAATVANANGSIGTADGTSFSCPIIAGMAASLWQAFPTKTNKE
CCCCCCCCCCCCCEECCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
IRQMILASADRYTTPDTSYGYGIPNFGSTLGTQTFLSTSDFLVYPNPTKSNISFLFDNET
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCHHEECCCCEEEECCCCCCCEEEEECCCC
ASVSIYSLLGQKLIEKQITNQNPVLSVEGLTNGLYFYTFDAGSLHKTGKIIKQ
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHCC
>Mature Secondary Structure
MRYNFTFLLFFVSFAIFAQEEAWVYFNAKPNAQAFFSNPLSELSQRALDRRTNQNIVLDL
CCEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEE
TDAPLEASFVNQIKSSTGITLMAQSKWLNALHIRGTQTNINALKGLSFVAKVEFADKSLN
CCCCCHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHHCCEEEEEEEECCCCCC
TTGKKVSVAKTSQTLDKLKTTVDYSYGNSANQIQMLNGQVLHQQNFTGEGKIIAVLDAGF
CCCCEEEEEHHHHHHHHHHHHEEECCCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCC
PGVNTAQPFENLRNNNRILGGYDFTTRNDNFYTGDDHGTLVLSTMGGYKENALVGTAPNA
CCCCCCHHHHHHHCCCEEEECEEEEECCCCEEECCCCCEEEEEECCCCCCCCEEECCCCC
SYYLFITEIDAVENPVEESLWVEAAERADALGVDIITTSLGYFGDRTEPRYNHTYSDMNG
EEEEEEEEECHHCCCHHHHHHHHHHHHCCCCCEEHEEHHHHCCCCCCCCCCCCCHHHHHH
ITTFISRGAEIAFSKGILVLASAGNEGNTTEKHVGSPADAVSVLAIGAVTASKVKSSFSS
HHHHHHCCCCEEEECCEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGPSYDNRIKPDVMAQGSAATVANANGSIGTADGTSFSCPIIAGMAASLWQAFPTKTNKE
CCCCCCCCCCCCCEECCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
IRQMILASADRYTTPDTSYGYGIPNFGSTLGTQTFLSTSDFLVYPNPTKSNISFLFDNET
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCHHEECCCCEEEECCCCCCCEEEEECCCC
ASVSIYSLLGQKLIEKQITNQNPVLSVEGLTNGLYFYTFDAGSLHKTGKIIKQ
CCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]