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The map label for this gene is glnA [H]

Identifier: 146300100

GI number: 146300100

Start: 2816329

End: 2818518

Strand: Direct

Name: glnA [H]

Synonym: Fjoh_2345

Alternate gene names: 146300100

Gene position: 2816329-2818518 (Clockwise)

Preceding gene: 146300097

Following gene: 146300101

Centisome position: 46.19

GC content: 35.02

Gene sequence:

>2190_bases
ATGTCAACATTACGTTTCCAAGCGCTAAAAGAAGCTTCTACCAGAAAGCCGGTGCATTTTGAAGAAATTGATAGAAAATC
TAATCTTTTTGGTTCAAATGTGTTTAATGAAAAAGCAATGAAGCAGTATTTAACTTCTGATGCTTTAAAAGGAGTAAGAG
ATGCCATTCAACATGGAACGAAGATAGAACGAAAACTGGCAGATTATATCGCCATGGGAATGAAAGAATGGGCATTATCA
AAAGGTGTTACACATTATACACACTGGTTTCAGCCGCTTACAGGAACAACAGCAGAAAAACATGATGCTTTTTTTGAAAC
GTCTTATGACGGCAATGAGTCTGTAGAAAAATTTGGCGGTGCACAATTAGTACAGCAGGAACCAGATGCATCAAGTTTCC
CAAATGGTGGAATTAGAAATACATTTGAAGCAAGAGGTTATACAGCCTGGGATCCAACTTCGCCTGCTTTTATATATGGT
ACAACTTTATGTATTCCAACTGTATTCATCGCTTATACTGGTGAAGCTTTAGATAATAAGATACCTTTATTAAGAGCATT
ATCTGCAATTGACGAAGCAGCTACAGAAGTTTGTAAATATTTTGACAAAAACGTAAAAAAGGTAACGGCAACATTAGGAT
GGGAGCAGGAATATTTCCTGATCGACAAAGCTTTGGCAAATTCTCGTCCAGACTTAATGATGACAGGAAGAACCTTATTA
GGTCATACTTCTGCAAAAGGACAGCAGTTAGACGATCACTATTTTGGATCTATCCCAACCCGCGCGCTTACTTATATGAG
AGATTTAGAGCAGGAATGTATGTTATTGGGTATTCCGGTAAAAACGCGTCATAATGAGGTTGCGCCAAATCAGTTTGAGT
TAGCACCAATTTTTGAAGAAACAAATCTTGCTGTAGATCATAACTCTTTATTAATGGATGTAATGCAGAGAGTAGCAGAA
CGTCACGACTTTAAAGTATTATTTCACGAAAAACCATTTAAAGGTGTAAACGGTTCTGGTAAACACAATAACTGGTCGCT
GGCAACAGATACAGGAGTTAATTTATTGAGTCCGAGCAAAACGCCAATGAGCAATTTACAATTTTTGACTTTCTTTATTA
ATACAATAAAAGCAGTAAACGATAACGAAACTTTATTAAGAGCTTCTATCGCAACAGCAAGTAACGATCACAGGTTAGGA
GCAAACGAAGCGCCTCCGGCAATTATGTCTGTATTTATTGGAGCGCAATTAACAAAAGTATTGTCTGAGTTAGAAAGTGT
AACAACAGGAAAACTTTCTCCAGAAGAAAAAACAGATCTTAAATTAAATGTAGTTGGGAAAATTCCAGACGTATTATTAG
ACAATACAGATCGCAACAGAACTTCGCCTTTTGCTTTTACAGGAAATAAATTTGAGTTTAGAGCCGTAGGTTCAAATTCA
AACTGTTCAAACGCAATGACCACTTTGAATGCGATTGTAGCAAAACAATTAATAGACTTTAAAAATGAAGTAGAGAACCT
AATTGAGTCGAAAGACATGAAAAAGGACGATGCTATTTTTAATGTTTTAAGAGAATACATCAAACAATCTAAAAAAATCC
TTTTTGAAGGAGACGGTTACAGCGAAGCTTGGGAAAAAGAAGCAGCAAAAAGAGGCTTAAGCAACTTTAAAACTACTCCG
GAAGCTATTAAGGCAAAAGTATCAAAACAAGCTTTGGAGTTATTTGCTGAACTTGGAATTTTTAACCACGTTGAAGCCGA
AGCCCGTTACGAAATTGAATTGGAAGAATACACAAAGAAAATCCAGATTGAAGGCAGAGTTTTAGGTGATATTGCCAGAA
ACCATGTAATTCCAACAGCAATTCGTTATCAGAATACTTTAATTGAAAATGTAAAAGGTTTAAAAGAAATCTTTGGAAAA
GAATTTGAAACCATTGCAAAAGAACAAATTACTTTAATAAAAGAAATTTCAGGTCATATTGAAGGAATCAATTCTAAAGT
ATTGGCAATGACTAATGAAAGAAAAAAAGCAAACCAATTGACTGATGCCCAAAAAATGGCCGAAGCATATTGCAATAAAG
TAAAACCTTATTTTGATGAAATTCGTAATCACTGTGATAAATTAGAATTATTAGTTGATGACGAAAGCTGGACACTAACA
AAATATAGAGAATTACTGTTCACGAAATAA

Upstream 100 bases:

>100_bases
ATAAAAATCTCGAATTTAAGTTTAAAACAGGGTTATTTATTGTTTATATTTGTCGATCATTTTTCAATGAAAATTATTAC
GAATTTTTAAAATTATAGAC

Downstream 100 bases:

>100_bases
GTTATATACTTAGAAATGTTAAAAACCTGTCTTGATAAAGACAGGTTTTTTTATGATAAAATGAAAAATTGGTCTAATTG
ATACTCATTTTAATAAATGT

Product: glutamine synthetase, catalytic region

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MSTLRFQALKEASTRKPVHFEEIDRKSNLFGSNVFNEKAMKQYLTSDALKGVRDAIQHGTKIERKLADYIAMGMKEWALS
KGVTHYTHWFQPLTGTTAEKHDAFFETSYDGNESVEKFGGAQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIYG
TTLCIPTVFIAYTGEALDNKIPLLRALSAIDEAATEVCKYFDKNVKKVTATLGWEQEYFLIDKALANSRPDLMMTGRTLL
GHTSAKGQQLDDHYFGSIPTRALTYMRDLEQECMLLGIPVKTRHNEVAPNQFELAPIFEETNLAVDHNSLLMDVMQRVAE
RHDFKVLFHEKPFKGVNGSGKHNNWSLATDTGVNLLSPSKTPMSNLQFLTFFINTIKAVNDNETLLRASIATASNDHRLG
ANEAPPAIMSVFIGAQLTKVLSELESVTTGKLSPEEKTDLKLNVVGKIPDVLLDNTDRNRTSPFAFTGNKFEFRAVGSNS
NCSNAMTTLNAIVAKQLIDFKNEVENLIESKDMKKDDAIFNVLREYIKQSKKILFEGDGYSEAWEKEAAKRGLSNFKTTP
EAIKAKVSKQALELFAELGIFNHVEAEARYEIELEEYTKKIQIEGRVLGDIARNHVIPTAIRYQNTLIENVKGLKEIFGK
EFETIAKEQITLIKEISGHIEGINSKVLAMTNERKKANQLTDAQKMAEAYCNKVKPYFDEIRNHCDKLELLVDDESWTLT
KYRELLFTK

Sequences:

>Translated_729_residues
MSTLRFQALKEASTRKPVHFEEIDRKSNLFGSNVFNEKAMKQYLTSDALKGVRDAIQHGTKIERKLADYIAMGMKEWALS
KGVTHYTHWFQPLTGTTAEKHDAFFETSYDGNESVEKFGGAQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIYG
TTLCIPTVFIAYTGEALDNKIPLLRALSAIDEAATEVCKYFDKNVKKVTATLGWEQEYFLIDKALANSRPDLMMTGRTLL
GHTSAKGQQLDDHYFGSIPTRALTYMRDLEQECMLLGIPVKTRHNEVAPNQFELAPIFEETNLAVDHNSLLMDVMQRVAE
RHDFKVLFHEKPFKGVNGSGKHNNWSLATDTGVNLLSPSKTPMSNLQFLTFFINTIKAVNDNETLLRASIATASNDHRLG
ANEAPPAIMSVFIGAQLTKVLSELESVTTGKLSPEEKTDLKLNVVGKIPDVLLDNTDRNRTSPFAFTGNKFEFRAVGSNS
NCSNAMTTLNAIVAKQLIDFKNEVENLIESKDMKKDDAIFNVLREYIKQSKKILFEGDGYSEAWEKEAAKRGLSNFKTTP
EAIKAKVSKQALELFAELGIFNHVEAEARYEIELEEYTKKIQIEGRVLGDIARNHVIPTAIRYQNTLIENVKGLKEIFGK
EFETIAKEQITLIKEISGHIEGINSKVLAMTNERKKANQLTDAQKMAEAYCNKVKPYFDEIRNHCDKLELLVDDESWTLT
KYRELLFTK
>Mature_728_residues
STLRFQALKEASTRKPVHFEEIDRKSNLFGSNVFNEKAMKQYLTSDALKGVRDAIQHGTKIERKLADYIAMGMKEWALSK
GVTHYTHWFQPLTGTTAEKHDAFFETSYDGNESVEKFGGAQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIYGT
TLCIPTVFIAYTGEALDNKIPLLRALSAIDEAATEVCKYFDKNVKKVTATLGWEQEYFLIDKALANSRPDLMMTGRTLLG
HTSAKGQQLDDHYFGSIPTRALTYMRDLEQECMLLGIPVKTRHNEVAPNQFELAPIFEETNLAVDHNSLLMDVMQRVAER
HDFKVLFHEKPFKGVNGSGKHNNWSLATDTGVNLLSPSKTPMSNLQFLTFFINTIKAVNDNETLLRASIATASNDHRLGA
NEAPPAIMSVFIGAQLTKVLSELESVTTGKLSPEEKTDLKLNVVGKIPDVLLDNTDRNRTSPFAFTGNKFEFRAVGSNSN
CSNAMTTLNAIVAKQLIDFKNEVENLIESKDMKKDDAIFNVLREYIKQSKKILFEGDGYSEAWEKEAAKRGLSNFKTTPE
AIKAKVSKQALELFAELGIFNHVEAEARYEIELEEYTKKIQIEGRVLGDIARNHVIPTAIRYQNTLIENVKGLKEIFGKE
FETIAKEQITLIKEISGHIEGINSKVLAMTNERKKANQLTDAQKMAEAYCNKVKPYFDEIRNHCDKLELLVDDESWTLTK
YRELLFTK

Specific function: Unknown

COG id: COG3968

COG function: function code R; Uncharacterized protein related to glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR022147 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF12437 GSIII_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 82106; Mature: 81975

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLRFQALKEASTRKPVHFEEIDRKSNLFGSNVFNEKAMKQYLTSDALKGVRDAIQHGT
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
KIERKLADYIAMGMKEWALSKGVTHYTHWFQPLTGTTAEKHDAFFETSYDGNESVEKFGG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHCCCEECCCCCCHHHHHHCC
AQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIYGTTLCIPTVFIAYTGEALDNK
HHHHHCCCCCCCCCCCCCCCCHHCCCEECCCCCCCCEEEHHHHHHHHHHHHHCCHHHCCC
IPLLRALSAIDEAATEVCKYFDKNVKKVTATLGWEQEYFLIDKALANSRPDLMMTGRTLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHH
GHTSAKGQQLDDHYFGSIPTRALTYMRDLEQECMLLGIPVKTRHNEVAPNQFELAPIFEE
CCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEECC
TNLAVDHNSLLMDVMQRVAERHDFKVLFHEKPFKGVNGSGKHNNWSLATDTGVNLLSPSK
CCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
TPMSNLQFLTFFINTIKAVNDNETLLRASIATASNDHRLGANEAPPAIMSVFIGAQLTKV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSELESVTTGKLSPEEKTDLKLNVVGKIPDVLLDNTDRNRTSPFAFTGNKFEFRAVGSNS
HHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCEEEECCCEEEEEECCCC
NCSNAMTTLNAIVAKQLIDFKNEVENLIESKDMKKDDAIFNVLREYIKQSKKILFEGDGY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCC
SEAWEKEAAKRGLSNFKTTPEAIKAKVSKQALELFAELGIFNHVEAEARYEIELEEYTKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEHHHHHHH
IQIEGRVLGDIARNHVIPTAIRYQNTLIENVKGLKEIFGKEFETIAKEQITLIKEISGHI
EEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGINSKVLAMTNERKKANQLTDAQKMAEAYCNKVKPYFDEIRNHCDKLELLVDDESWTLT
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHH
KYRELLFTK
HHHHHHCCC
>Mature Secondary Structure 
STLRFQALKEASTRKPVHFEEIDRKSNLFGSNVFNEKAMKQYLTSDALKGVRDAIQHGT
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
KIERKLADYIAMGMKEWALSKGVTHYTHWFQPLTGTTAEKHDAFFETSYDGNESVEKFGG
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHCCCEECCCCCCHHHHHHCC
AQLVQQEPDASSFPNGGIRNTFEARGYTAWDPTSPAFIYGTTLCIPTVFIAYTGEALDNK
HHHHHCCCCCCCCCCCCCCCCHHCCCEECCCCCCCCEEEHHHHHHHHHHHHHCCHHHCCC
IPLLRALSAIDEAATEVCKYFDKNVKKVTATLGWEQEYFLIDKALANSRPDLMMTGRTLL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHH
GHTSAKGQQLDDHYFGSIPTRALTYMRDLEQECMLLGIPVKTRHNEVAPNQFELAPIFEE
CCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEEECC
TNLAVDHNSLLMDVMQRVAERHDFKVLFHEKPFKGVNGSGKHNNWSLATDTGVNLLSPSK
CCEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCC
TPMSNLQFLTFFINTIKAVNDNETLLRASIATASNDHRLGANEAPPAIMSVFIGAQLTKV
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSELESVTTGKLSPEEKTDLKLNVVGKIPDVLLDNTDRNRTSPFAFTGNKFEFRAVGSNS
HHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCEEEECCCEEEEEECCCC
NCSNAMTTLNAIVAKQLIDFKNEVENLIESKDMKKDDAIFNVLREYIKQSKKILFEGDGY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEECCCC
SEAWEKEAAKRGLSNFKTTPEAIKAKVSKQALELFAELGIFNHVEAEARYEIELEEYTKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEEEHHHHHHH
IQIEGRVLGDIARNHVIPTAIRYQNTLIENVKGLKEIFGKEFETIAKEQITLIKEISGHI
EEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGINSKVLAMTNERKKANQLTDAQKMAEAYCNKVKPYFDEIRNHCDKLELLVDDESWTLT
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCHHH
KYRELLFTK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2576872 [H]