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The map label for this gene is folE

Identifier: 146300025

GI number: 146300025

Start: 2735506

End: 2736177

Strand: Reverse

Name: folE

Synonym: Fjoh_2270

Alternate gene names: 146300025

Gene position: 2736177-2735506 (Counterclockwise)

Preceding gene: 146300026

Following gene: 146300024

Centisome position: 44.88

GC content: 33.93

Gene sequence:

>672_bases
ATGATAAATAACGAAGATTTTTTAGACGAAATAGGTGACAGTCATTTCAGCAGTAATGCAAAAAACCCTTTAAGAGAGGA
TGCTTTTGATATAACTGATGAAGAAAAAATAGAAAAAATTAAAAAAGATGTTGAAAACATTCTTCAGACTCTTGGAATGG
ATTTAACAGATGACAGCATAAAAGGTACTCCAAACCGAGTAGCTAAAATGTTTGTAAAAGAAATTTTTGGAGGTTTAAAT
CCTGCAAAACAGCCAAAAGCCTCAACTTTTGACAATAATTACAAATATGGCGAAATGCTGGTAGAAAAAAACATTACCGT
TTATTCTACCTGTGAACACCACTTACTGCCAATTATAGGACGTGCTCACGTTGCTTATATTTCAAGCGGAAGAGTTATTG
GTTTATCAAAAATGAACCGTATCGTTGAGTATTATGCTAAAAGACCTCAGGTACAAGAGCGTTTAACAATGCAGATTGTT
CAGGAACTTCAAAAAGCTTTAGGAACAGAAGATGTAGCCTGCGTTATCGATGCAAAACACCTTTGTGTTAACTCAAGAGG
AATCAAAGATATCGAAAGCAGTACTGTAACTTCTGAATTCGGCGGAAAATTCAAAGATCCTCAAACAAAAAGAGAATTTC
TTGATTACATTAAACTAGATACACAGTTCTAA

Upstream 100 bases:

>100_bases
AAAGGATCGATTAAATAATAAAAACGATAGAAAAGGTTTCCCATTTATTAATTATCTTTACCGCCTTAAAAAGATATACC
CATTATAATAGTAGTTTTTA

Downstream 100 bases:

>100_bases
AAGTTTATTGTTTTTAGTTAATAGTTTATTGTTCTTATGGCGAAAATTGAAAAATTTGAAGATCTGGAAATCTGGAGACT
TGCTCGAGAAATTTGTCAGC

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MINNEDFLDEIGDSHFSSNAKNPLREDAFDITDEEKIEKIKKDVENILQTLGMDLTDDSIKGTPNRVAKMFVKEIFGGLN
PAKQPKASTFDNNYKYGEMLVEKNITVYSTCEHHLLPIIGRAHVAYISSGRVIGLSKMNRIVEYYAKRPQVQERLTMQIV
QELQKALGTEDVACVIDAKHLCVNSRGIKDIESSTVTSEFGGKFKDPQTKREFLDYIKLDTQF

Sequences:

>Translated_223_residues
MINNEDFLDEIGDSHFSSNAKNPLREDAFDITDEEKIEKIKKDVENILQTLGMDLTDDSIKGTPNRVAKMFVKEIFGGLN
PAKQPKASTFDNNYKYGEMLVEKNITVYSTCEHHLLPIIGRAHVAYISSGRVIGLSKMNRIVEYYAKRPQVQERLTMQIV
QELQKALGTEDVACVIDAKHLCVNSRGIKDIESSTVTSEFGGKFKDPQTKREFLDYIKLDTQF
>Mature_223_residues
MINNEDFLDEIGDSHFSSNAKNPLREDAFDITDEEKIEKIKKDVENILQTLGMDLTDDSIKGTPNRVAKMFVKEIFGGLN
PAKQPKASTFDNNYKYGEMLVEKNITVYSTCEHHLLPIIGRAHVAYISSGRVIGLSKMNRIVEYYAKRPQVQERLTMQIV
QELQKALGTEDVACVIDAKHLCVNSRGIKDIESSTVTSEFGGKFKDPQTKREFLDYIKLDTQF

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=181, Percent_Identity=39.7790055248619, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI4503949, Length=181, Percent_Identity=39.7790055248619, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI66932970, Length=169, Percent_Identity=34.3195266272189, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI66932972, Length=140, Percent_Identity=38.5714285714286, Blast_Score=93, Evalue=1e-19,
Organism=Escherichia coli, GI1788476, Length=199, Percent_Identity=49.2462311557789, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17560486, Length=223, Percent_Identity=36.322869955157, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321706, Length=189, Percent_Identity=39.6825396825397, Blast_Score=135, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24656782, Length=221, Percent_Identity=34.841628959276, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656796, Length=209, Percent_Identity=34.9282296650718, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656791, Length=197, Percent_Identity=34.010152284264, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_FLAJ1 (A5FHM7)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194616.1
- ProteinModelPortal:   A5FHM7
- SMR:   A5FHM7
- STRING:   A5FHM7
- GeneID:   5094405
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2270
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   RSRQEFF
- ProtClustDB:   CLSK847441
- BioCyc:   FJOH376686:FJOH_2270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 25313; Mature: 25313

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINNEDFLDEIGDSHFSSNAKNPLREDAFDITDEEKIEKIKKDVENILQTLGMDLTDDSI
CCCCHHHHHHHCCHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGTPNRVAKMFVKEIFGGLNPAKQPKASTFDNNYKYGEMLVEKNITVYSTCEHHLLPIIG
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHH
RAHVAYISSGRVIGLSKMNRIVEYYAKRPQVQERLTMQIVQELQKALGTEDVACVIDAKH
HHHHEEECCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
LCVNSRGIKDIESSTVTSEFGGKFKDPQTKREFLDYIKLDTQF
HHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINNEDFLDEIGDSHFSSNAKNPLREDAFDITDEEKIEKIKKDVENILQTLGMDLTDDSI
CCCCHHHHHHHCCHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
KGTPNRVAKMFVKEIFGGLNPAKQPKASTFDNNYKYGEMLVEKNITVYSTCEHHLLPIIG
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHH
RAHVAYISSGRVIGLSKMNRIVEYYAKRPQVQERLTMQIVQELQKALGTEDVACVIDAKH
HHHHEEECCCCEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
LCVNSRGIKDIESSTVTSEFGGKFKDPQTKREFLDYIKLDTQF
HHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA