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The map label for this gene is cysS

Identifier: 146300023

GI number: 146300023

Start: 2733543

End: 2735021

Strand: Reverse

Name: cysS

Synonym: Fjoh_2268

Alternate gene names: 146300023

Gene position: 2735021-2733543 (Counterclockwise)

Preceding gene: 146300024

Following gene: 146300022

Centisome position: 44.86

GC content: 37.05

Gene sequence:

>1479_bases
ATGCCACTATACAGCAGTCAGCCACTAAAAATATACAATTCACTTTCGGGTGAAAAAGAAAATTTCAAACCAATCCATGA
AGGAAATGTTGGAATGTATGTTTGTGGACCAACGGTTTACAGCAATGTGCATTTAGGAAACGTAAGGACTTTTATGTCTT
TTGACGTGATTTTCAGGTATTTTTTACACCTTGATTATAAAGTTCGTTACGTTCGAAATATTACTGATGTTGGTCATATT
GTAGATGATGTTGATGAAGGAGAAGATAAAATTGCCAAAAAAGCACGTTTAGAGCAGTTAGAACCAATGGAAGTTGTGCA
GCGTTATACTGTTGACTTTCATGACATTTTAAAAGCTTTCAACTTTTTACCGCCAAGTATTGAGCCAACAGCAACCGGAC
ATATTATTGAACAAATCGAAATTATCAAAAAAATTATCGATACCGGAATTGGTTACGAAGCCAACGGATCTGTTTATTTT
GATGTTGTAAAATATAACGAAACCAATAATTACGGCGTTTTAAGCGGCAGAAACATCGAAGATATGCTGGCGAATACCCG
TGATCTTGACGGACAGTCAGACAAAAGAAATCCGCAGGATTTTGCACTTTGGAAAAAAGCAGAACCGGAACATATCATGA
GATGGCCTTCACCTTGGAGTGATGGTTTCCCTGGCTGGCACTTAGAATGTACTGCAATGAGTACTAAATATCTAGGAAAT
CATTTTGACATTCACGGCGGCGGAATGGATTTGAAATTCCCTCATCACGAATGTGAAATTGCTCAAAACGAAGCCTGCAC
AGGACAATCGCCGGTAAATTACTGGATGCACACTAATATGCTTACGCTGAACGGAAAGAAAATGGCAAAATCTACCGGAA
ACAATATTTTACCGGGAGAAATTTTAAGCGGCGACAATACGATTTTAAGCAAAGCGTTTTCTGCATCAGTTACAAGATTC
TTTATGCTTCAGGCACATTACAGAAGTATTTTAGATTTTTCTGATGATGCGATTACTGCTGCCGAAAAAGGATACAAAAG
ATTAATGGAAGCTCTTGAAGCTTTACCAGCAATAAAAGCAGGTTCTTCCAGCTCTGTTGATTTTGCAGCATGGAAACAAG
CTTGCTATGACGCCATGAATGATGATTTTAACACTCCAATTTTAATTGCACAATTATTTGAAGCTGTTCGTTATATCAAT
TTACTAAAAGAAGGAAAAGAAACTATTTCTGCCGAAGATTTAAAATCTTTTTCAACTGCCATAAATGCGTTTGTTTTTGA
CGTTTTAGGTTTAAGCGATGAGAAAGCAGCAGACGGCAATAACGATAAATTAGAAGGTGTTGTAAATATGCTTATTGGAA
TGAGAAATCAAGCCAGAGCAGATAAAAATTTCGCACTTTCTGACCAAATCCGCGATCAGTTAATTGCGCTTGGAATTCAG
TTAAAAGACGGCAAAGAAGGAACGAGTTTTTCAATTTAA

Upstream 100 bases:

>100_bases
TATTTAAACAAATGTGAAATCAAGGGTCTAAAATTCAAATAAAATTTGCAACCAAAACAATAAACAATAAACCACGAACA
ACAAACAATTAAGTAAAAAT

Downstream 100 bases:

>100_bases
GAAAGAAAAAATCTAATAAATCATGAAAGTCATTACGCCATTTGTTTTATTAGTACGCTTTTATCAAACTGCGATTTCAC
CTTTTACACCTGCTTCATGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MPLYSSQPLKIYNSLSGEKENFKPIHEGNVGMYVCGPTVYSNVHLGNVRTFMSFDVIFRYFLHLDYKVRYVRNITDVGHI
VDDVDEGEDKIAKKARLEQLEPMEVVQRYTVDFHDILKAFNFLPPSIEPTATGHIIEQIEIIKKIIDTGIGYEANGSVYF
DVVKYNETNNYGVLSGRNIEDMLANTRDLDGQSDKRNPQDFALWKKAEPEHIMRWPSPWSDGFPGWHLECTAMSTKYLGN
HFDIHGGGMDLKFPHHECEIAQNEACTGQSPVNYWMHTNMLTLNGKKMAKSTGNNILPGEILSGDNTILSKAFSASVTRF
FMLQAHYRSILDFSDDAITAAEKGYKRLMEALEALPAIKAGSSSSVDFAAWKQACYDAMNDDFNTPILIAQLFEAVRYIN
LLKEGKETISAEDLKSFSTAINAFVFDVLGLSDEKAADGNNDKLEGVVNMLIGMRNQARADKNFALSDQIRDQLIALGIQ
LKDGKEGTSFSI

Sequences:

>Translated_492_residues
MPLYSSQPLKIYNSLSGEKENFKPIHEGNVGMYVCGPTVYSNVHLGNVRTFMSFDVIFRYFLHLDYKVRYVRNITDVGHI
VDDVDEGEDKIAKKARLEQLEPMEVVQRYTVDFHDILKAFNFLPPSIEPTATGHIIEQIEIIKKIIDTGIGYEANGSVYF
DVVKYNETNNYGVLSGRNIEDMLANTRDLDGQSDKRNPQDFALWKKAEPEHIMRWPSPWSDGFPGWHLECTAMSTKYLGN
HFDIHGGGMDLKFPHHECEIAQNEACTGQSPVNYWMHTNMLTLNGKKMAKSTGNNILPGEILSGDNTILSKAFSASVTRF
FMLQAHYRSILDFSDDAITAAEKGYKRLMEALEALPAIKAGSSSSVDFAAWKQACYDAMNDDFNTPILIAQLFEAVRYIN
LLKEGKETISAEDLKSFSTAINAFVFDVLGLSDEKAADGNNDKLEGVVNMLIGMRNQARADKNFALSDQIRDQLIALGIQ
LKDGKEGTSFSI
>Mature_491_residues
PLYSSQPLKIYNSLSGEKENFKPIHEGNVGMYVCGPTVYSNVHLGNVRTFMSFDVIFRYFLHLDYKVRYVRNITDVGHIV
DDVDEGEDKIAKKARLEQLEPMEVVQRYTVDFHDILKAFNFLPPSIEPTATGHIIEQIEIIKKIIDTGIGYEANGSVYFD
VVKYNETNNYGVLSGRNIEDMLANTRDLDGQSDKRNPQDFALWKKAEPEHIMRWPSPWSDGFPGWHLECTAMSTKYLGNH
FDIHGGGMDLKFPHHECEIAQNEACTGQSPVNYWMHTNMLTLNGKKMAKSTGNNILPGEILSGDNTILSKAFSASVTRFF
MLQAHYRSILDFSDDAITAAEKGYKRLMEALEALPAIKAGSSSSVDFAAWKQACYDAMNDDFNTPILIAQLFEAVRYINL
LKEGKETISAEDLKSFSTAINAFVFDVLGLSDEKAADGNNDKLEGVVNMLIGMRNQARADKNFALSDQIRDQLIALGIQL
KDGKEGTSFSI

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=528, Percent_Identity=33.9015151515151, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI21269877, Length=419, Percent_Identity=31.7422434367542, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI303304987, Length=419, Percent_Identity=31.7422434367542, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI10835051, Length=419, Percent_Identity=31.7422434367542, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI62240992, Length=419, Percent_Identity=31.7422434367542, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1786737, Length=492, Percent_Identity=41.0569105691057, Blast_Score=335, Evalue=5e-93,
Organism=Caenorhabditis elegans, GI17509795, Length=254, Percent_Identity=34.6456692913386, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17509797, Length=254, Percent_Identity=34.6456692913386, Blast_Score=145, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6324082, Length=425, Percent_Identity=30.3529411764706, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI19922218, Length=545, Percent_Identity=29.9082568807339, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI19922364, Length=288, Percent_Identity=36.4583333333333, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_FLAJ1 (A5FHM5)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194614.1
- ProteinModelPortal:   A5FHM5
- SMR:   A5FHM5
- STRING:   A5FHM5
- GeneID:   5094403
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_2268
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   FJOH376686:FJOH_2268-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55212; Mature: 55081

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYSSQPLKIYNSLSGEKENFKPIHEGNVGMYVCGPTVYSNVHLGNVRTFMSFDVIFRY
CCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHCCCEEECCHHHHHHHHHHHHH
FLHLDYKVRYVRNITDVGHIVDDVDEGEDKIAKKARLEQLEPMEVVQRYTVDFHDILKAF
HHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
NFLPPSIEPTATGHIIEQIEIIKKIIDTGIGYEANGSVYFDVVKYNETNNYGVLSGRNIE
HCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEECCCCCEEEECCCCHH
DMLANTRDLDGQSDKRNPQDFALWKKAEPEHIMRWPSPWSDGFPGWHLECTAMSTKYLGN
HHHCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEHHHHCCC
HFDIHGGGMDLKFPHHECEIAQNEACTGQSPVNYWMHTNMLTLNGKKMAKSTGNNILPGE
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCEEEECCHHHHHHCCCCCCCCC
ILSGDNTILSKAFSASVTRFFMLQAHYRSILDFSDDAITAAEKGYKRLMEALEALPAIKA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GSSSSVDFAAWKQACYDAMNDDFNTPILIAQLFEAVRYINLLKEGKETISAEDLKSFSTA
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
INAFVFDVLGLSDEKAADGNNDKLEGVVNMLIGMRNQARADKNFALSDQIRDQLIALGIQ
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCE
LKDGKEGTSFSI
EECCCCCCCCCC
>Mature Secondary Structure 
PLYSSQPLKIYNSLSGEKENFKPIHEGNVGMYVCGPTVYSNVHLGNVRTFMSFDVIFRY
CCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHCCCEEECCHHHHHHHHHHHHH
FLHLDYKVRYVRNITDVGHIVDDVDEGEDKIAKKARLEQLEPMEVVQRYTVDFHDILKAF
HHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
NFLPPSIEPTATGHIIEQIEIIKKIIDTGIGYEANGSVYFDVVKYNETNNYGVLSGRNIE
HCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEECCCCCEEEECCCCHH
DMLANTRDLDGQSDKRNPQDFALWKKAEPEHIMRWPSPWSDGFPGWHLECTAMSTKYLGN
HHHCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEHHHHCCC
HFDIHGGGMDLKFPHHECEIAQNEACTGQSPVNYWMHTNMLTLNGKKMAKSTGNNILPGE
EEEECCCCCCCCCCCHHHHHCCCCCCCCCCCCEEEEECCEEEECCHHHHHHCCCCCCCCC
ILSGDNTILSKAFSASVTRFFMLQAHYRSILDFSDDAITAAEKGYKRLMEALEALPAIKA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
GSSSSVDFAAWKQACYDAMNDDFNTPILIAQLFEAVRYINLLKEGKETISAEDLKSFSTA
CCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
INAFVFDVLGLSDEKAADGNNDKLEGVVNMLIGMRNQARADKNFALSDQIRDQLIALGIQ
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHCCE
LKDGKEGTSFSI
EECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA