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The map label for this gene is nagB [C]

Identifier: 146299788

GI number: 146299788

Start: 2381279

End: 2383192

Strand: Direct

Name: nagB [C]

Synonym: Fjoh_2029

Alternate gene names: 146299788

Gene position: 2381279-2383192 (Clockwise)

Preceding gene: 146299787

Following gene: 146299791

Centisome position: 39.06

GC content: 36.83

Gene sequence:

>1914_bases
ATGATTAAAGAAAGTATAAATTTCAAAGAAGCAGGAAAGTTTGAAGAAACGCGTTTTGAAAAGATACACAATGTAATTTT
TGATTCGTCTAAAGAAGCTTCGATTTTAGTAGCTCAGGAAATAGCCAATATAATTCAGAGAAAAGAAGAATTAAACGAAC
CTTGTGTTTTAGGTTTGGCAACAGGATCTTCTCCTATAAAAGTATACGAAGAATTAGTTCGTCTTCATAAAGAAGAAGGT
TTAAGCTTTGCAAATGTGGTAACTTTCAACTTAGATGAGTATTATCCAATGGATAAAAACGACATTCAAAGTTATTGGTA
CTTTATGCATGAGCACCTATTTAATCATGTTAATATTCCGCCTCAAAATATCAATATTCCTGACGGAAGCATCAGCAATG
AAGACCTTCAGCAATATTGCATCGATTATGAAATGAAAATCAAAGCTTACGGCGGATTGGATTTTCAGCTTCTTGGAATT
GGAAGAACGGGACATATCGGATTCAACGAACCGGGATCTCACGTAAATTCTGGAACCAGAAGCATTACATTAGATCACTT
AACACGTGTTGATGCAGCATCATCATTTTTAGGAATTGATAATGTGCCTAGAAAAGCAATTACAATGGGAATTGGCACCG
TAAAAAATGCCAAAAGAATTGTACTTCTTGGATGGGGAATCAGCAAAGCAGATATTATAAAGAAAACAATCGAAGGTGAA
ATTTCATCTCGAGTTCCGGCGACGTATTTACAACAGCACAACAACACAACTTTTGTTTTAGATACCGAAGCTTCATCAGA
ATTAACCCGAGTAAAAACGCCTTGGTTAGTAAAATCTGTAATCTGGACAGACGAATTAAAATTGAAAGCGGTAGCTTGGT
TAAGCGAATTAACTAAAAAACCTTTCTTAAAACTGACTGACAAAGATTATAACGATAACGGAATGTCTAGCGTTTTAACA
GAAGAAGGGACTGCATACAATTTGAATATTAAAATGTTCAACAAAATGCAGCAGACTATCACGGGATGGCCGGGAGGAAA
ACCAAATGCTGATGATACCTACAGACCAGAACGTGCAACTCCGGAAAGAAAAAGAATTATCATTTTTAGTCCGCACCCAG
ATGATGATGTTATCTCAATGGGAGGAACTTTTGACAGATTGGTAGAGCAGGGGCATGATGTTCATATTGCATACCAGACT
TCAGGAAATATTGCGGTTTCGAATCAGGAAGCTTTAAAGTTTGCTGAAATTACAAAAGCATTAAATGCTGATTCTGTTAC
GGCAGAGACTATTATTGACTTTTTGAAAAACAAAAAAAGCAATGATATCGATTCATTAGAAGTAAGAAAGCTGAAAGGTT
TAATTAGAAGAAGTGAATCATTAGCAGCAACAAGATATTTAGGCTTGCCGGATTCTAATGTAAATTTCCTTGATCTTCCT
TTTTATGAAACAGGAACGGTAAAGAAAAATAATCTTGGTGAGGCAGATATTGAAATTATGTGCGATATCATCGAAAGAAT
AAAACCTCATCAAATCTACGCAGCAGGAGATTTAGCAGACCCGCACGGAACGCACAAAGTGTGTCTGGATAGTTTGTTTG
AAGCTTTGAAACGATTGAAACACAAGAGTTTTATGGATGACTGCTGGGTTTGGCTGTACAGAGGTGCTTGGCACGAATGG
GAAACCTACCAAATTGAAATGGCGGTTCCAATGAGTCCGGATCAGGTGCTTAAAAAACGTCACGCGATTTTCTATCACCA
GTCTCAAAAAGACGGTGTAATGTTTCAGGGTGATGACAGCAGAGAATTCTGGATTAGAGCAGAAGACAGAAACAGATTAA
CTGCAGAAAAATATCACGCTCTAGGTTTAGCTGACTATTCAGCAATTGAAGCCTTCAAGAGATATTTCTTCTAA

Upstream 100 bases:

>100_bases
TTTTGTCCTATAGCGAAGGTAACAGGTTATTAGTCGAATTTTTGATTATTTACATTATGTAATATTTAATTTTATACTAA
ATAATTAGAACAACAGGCAA

Downstream 100 bases:

>100_bases
GAAAATCTTTAAAATATAATAAACTATTTTCATGTGGTTTATTTGGTTAGTTACCAAGTTTTGAAATGCAGCCGTGGTTG
GCTGCATTTTTTTTGTTTTT

Product: glucosamine-6-phosphate deaminase-like protein

Products: D-fructose 6-phosphate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MIKESINFKEAGKFEETRFEKIHNVIFDSSKEASILVAQEIANIIQRKEELNEPCVLGLATGSSPIKVYEELVRLHKEEG
LSFANVVTFNLDEYYPMDKNDIQSYWYFMHEHLFNHVNIPPQNINIPDGSISNEDLQQYCIDYEMKIKAYGGLDFQLLGI
GRTGHIGFNEPGSHVNSGTRSITLDHLTRVDAASSFLGIDNVPRKAITMGIGTVKNAKRIVLLGWGISKADIIKKTIEGE
ISSRVPATYLQQHNNTTFVLDTEASSELTRVKTPWLVKSVIWTDELKLKAVAWLSELTKKPFLKLTDKDYNDNGMSSVLT
EEGTAYNLNIKMFNKMQQTITGWPGGKPNADDTYRPERATPERKRIIIFSPHPDDDVISMGGTFDRLVEQGHDVHIAYQT
SGNIAVSNQEALKFAEITKALNADSVTAETIIDFLKNKKSNDIDSLEVRKLKGLIRRSESLAATRYLGLPDSNVNFLDLP
FYETGTVKKNNLGEADIEIMCDIIERIKPHQIYAAGDLADPHGTHKVCLDSLFEALKRLKHKSFMDDCWVWLYRGAWHEW
ETYQIEMAVPMSPDQVLKKRHAIFYHQSQKDGVMFQGDDSREFWIRAEDRNRLTAEKYHALGLADYSAIEAFKRYFF

Sequences:

>Translated_637_residues
MIKESINFKEAGKFEETRFEKIHNVIFDSSKEASILVAQEIANIIQRKEELNEPCVLGLATGSSPIKVYEELVRLHKEEG
LSFANVVTFNLDEYYPMDKNDIQSYWYFMHEHLFNHVNIPPQNINIPDGSISNEDLQQYCIDYEMKIKAYGGLDFQLLGI
GRTGHIGFNEPGSHVNSGTRSITLDHLTRVDAASSFLGIDNVPRKAITMGIGTVKNAKRIVLLGWGISKADIIKKTIEGE
ISSRVPATYLQQHNNTTFVLDTEASSELTRVKTPWLVKSVIWTDELKLKAVAWLSELTKKPFLKLTDKDYNDNGMSSVLT
EEGTAYNLNIKMFNKMQQTITGWPGGKPNADDTYRPERATPERKRIIIFSPHPDDDVISMGGTFDRLVEQGHDVHIAYQT
SGNIAVSNQEALKFAEITKALNADSVTAETIIDFLKNKKSNDIDSLEVRKLKGLIRRSESLAATRYLGLPDSNVNFLDLP
FYETGTVKKNNLGEADIEIMCDIIERIKPHQIYAAGDLADPHGTHKVCLDSLFEALKRLKHKSFMDDCWVWLYRGAWHEW
ETYQIEMAVPMSPDQVLKKRHAIFYHQSQKDGVMFQGDDSREFWIRAEDRNRLTAEKYHALGLADYSAIEAFKRYFF
>Mature_637_residues
MIKESINFKEAGKFEETRFEKIHNVIFDSSKEASILVAQEIANIIQRKEELNEPCVLGLATGSSPIKVYEELVRLHKEEG
LSFANVVTFNLDEYYPMDKNDIQSYWYFMHEHLFNHVNIPPQNINIPDGSISNEDLQQYCIDYEMKIKAYGGLDFQLLGI
GRTGHIGFNEPGSHVNSGTRSITLDHLTRVDAASSFLGIDNVPRKAITMGIGTVKNAKRIVLLGWGISKADIIKKTIEGE
ISSRVPATYLQQHNNTTFVLDTEASSELTRVKTPWLVKSVIWTDELKLKAVAWLSELTKKPFLKLTDKDYNDNGMSSVLT
EEGTAYNLNIKMFNKMQQTITGWPGGKPNADDTYRPERATPERKRIIIFSPHPDDDVISMGGTFDRLVEQGHDVHIAYQT
SGNIAVSNQEALKFAEITKALNADSVTAETIIDFLKNKKSNDIDSLEVRKLKGLIRRSESLAATRYLGLPDSNVNFLDLP
FYETGTVKKNNLGEADIEIMCDIIERIKPHQIYAAGDLADPHGTHKVCLDSLFEALKRLKHKSFMDDCWVWLYRGAWHEW
ETYQIEMAVPMSPDQVLKKRHAIFYHQSQKDGVMFQGDDSREFWIRAEDRNRLTAEKYHALGLADYSAIEAFKRYFF

Specific function: Catalyzes The Reversible Isomerization-Deamination Of Glucosamine 6-Phosphate (Glcn6p) To Form Fructose 6-Phosphate (Fru6p) And Ammonium Ion. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=259, Percent_Identity=41.6988416988417, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI13027378, Length=251, Percent_Identity=40.6374501992032, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1786893, Length=239, Percent_Identity=40.5857740585774, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789530, Length=212, Percent_Identity=25.4716981132075, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994958, Length=236, Percent_Identity=27.1186440677966, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554876, Length=259, Percent_Identity=37.4517374517375, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24581960, Length=228, Percent_Identity=40.7894736842105, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920764, Length=228, Percent_Identity=40.7894736842105, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR003737
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso; PF02585 PIG-L [H]

EC number: 3.5.99.6

Molecular weight: Translated: 72521; Mature: 72521

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKESINFKEAGKFEETRFEKIHNVIFDSSKEASILVAQEIANIIQRKEELNEPCVLGLA
CCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEE
TGSSPIKVYEELVRLHKEEGLSFANVVTFNLDEYYPMDKNDIQSYWYFMHEHLFNHVNIP
CCCCHHHHHHHHHHHHHHHCCCEEEEEEEECHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
PQNINIPDGSISNEDLQQYCIDYEMKIKAYGGLDFQLLGIGRTGHIGFNEPGSHVNSGTR
CCCCCCCCCCCCHHHHHHHHCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCC
SITLDHLTRVDAASSFLGIDNVPRKAITMGIGTVKNAKRIVLLGWGISKADIIKKTIEGE
EEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCH
ISSRVPATYLQQHNNTTFVLDTEASSELTRVKTPWLVKSVIWTDELKLKAVAWLSELTKK
HHCCCCHHHHHHCCCEEEEEECCCCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
PFLKLTDKDYNDNGMSSVLTEEGTAYNLNIKMFNKMQQTITGWPGGKPNADDTYRPERAT
CCEEECCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PERKRIIIFSPHPDDDVISMGGTFDRLVEQGHDVHIAYQTSGNIAVSNQEALKFAEITKA
CCCCEEEEECCCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHH
LNADSVTAETIIDFLKNKKSNDIDSLEVRKLKGLIRRSESLAATRYLGLPDSNVNFLDLP
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECC
FYETGTVKKNNLGEADIEIMCDIIERIKPHQIYAAGDLADPHGTHKVCLDSLFEALKRLK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
HKSFMDDCWVWLYRGAWHEWETYQIEMAVPMSPDQVLKKRHAIFYHQSQKDGVMFQGDDS
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHEEEECCCCCCEEEECCCC
REFWIRAEDRNRLTAEKYHALGLADYSAIEAFKRYFF
CEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIKESINFKEAGKFEETRFEKIHNVIFDSSKEASILVAQEIANIIQRKEELNEPCVLGLA
CCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEE
TGSSPIKVYEELVRLHKEEGLSFANVVTFNLDEYYPMDKNDIQSYWYFMHEHLFNHVNIP
CCCCHHHHHHHHHHHHHHHCCCEEEEEEEECHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
PQNINIPDGSISNEDLQQYCIDYEMKIKAYGGLDFQLLGIGRTGHIGFNEPGSHVNSGTR
CCCCCCCCCCCCHHHHHHHHCCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCC
SITLDHLTRVDAASSFLGIDNVPRKAITMGIGTVKNAKRIVLLGWGISKADIIKKTIEGE
EEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCH
ISSRVPATYLQQHNNTTFVLDTEASSELTRVKTPWLVKSVIWTDELKLKAVAWLSELTKK
HHCCCCHHHHHHCCCEEEEEECCCCCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
PFLKLTDKDYNDNGMSSVLTEEGTAYNLNIKMFNKMQQTITGWPGGKPNADDTYRPERAT
CCEEECCCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
PERKRIIIFSPHPDDDVISMGGTFDRLVEQGHDVHIAYQTSGNIAVSNQEALKFAEITKA
CCCCEEEEECCCCCCCEEECCCHHHHHHHCCCCEEEEEECCCCEEECCHHHHHHHHHHHH
LNADSVTAETIIDFLKNKKSNDIDSLEVRKLKGLIRRSESLAATRYLGLPDSNVNFLDLP
CCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECC
FYETGTVKKNNLGEADIEIMCDIIERIKPHQIYAAGDLADPHGTHKVCLDSLFEALKRLK
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
HKSFMDDCWVWLYRGAWHEWETYQIEMAVPMSPDQVLKKRHAIFYHQSQKDGVMFQGDDS
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHEEEECCCCCCEEEECCCC
REFWIRAEDRNRLTAEKYHALGLADYSAIEAFKRYFF
CEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucosamine 6-phosphate; H2O

Specific reaction: D-glucosamine 6-phosphate + H2O = D-fructose 6-phosphate + NH3

General reaction: Group transfer (transmolecular Intramolecular oxidoreduction) [C]

Inhibitor: N3-(4-Methoxyfumaroyl)-L-2, 3-diaminopropanoic acid [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]