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The map label for this gene is mraW

Identifier: 146299563

GI number: 146299563

Start: 2083565

End: 2084473

Strand: Direct

Name: mraW

Synonym: Fjoh_1803

Alternate gene names: 146299563

Gene position: 2083565-2084473 (Clockwise)

Preceding gene: 146299562

Following gene: 146299564

Centisome position: 34.17

GC content: 36.96

Gene sequence:

>909_bases
ATGACGACGACAATGGAATATCATAATCCGGTTTTACTTCATCCAACGGTTGATGGTTTAGATATTAAGCCAGACGGCAT
TTATGTAGATGTAACGTTTGGCGGCGGCGGGCACTCAAAAGAAATTTTGAGACGTTTAGGGCCAAACGGAAAATTGTTTG
CTTTTGATCAGGATGAAGATGCGCTGGCAAATGCGCTGCCTGATGAGAGGTTTACCTTAATTAATGAGAATTTTAGATTT
ATAAAAAGATTTCTTCGTTTTCATGGAATAAAAGCAGTAGATGGGATCTTGGCAGATTTAGGAGTTTCATCACATCAGTT
CGATGTTCCGGAGAGAGGTTTTTCAACAAGATTCGATGCTGAGCTGGATATGCGGATGAGTCAAAAAAATGACTTAAATG
CTTACCGGGTGGTAAATGAATATGAAGAACAGGATTTACGTCGTGTTTTTTTTGATTATGGGGAATTGAAAAATGCACCA
GTGCTGGCAAGAACAATTGTAGAAGCCAGACATCATAGACCAATAAAAACGACCGATGAATTAAAAGAAGTTTTGAAAAA
ATATTTACCGGAGAAAGTTCGAAATAAAATATTGGCTCAAATCTATCAGGCGATTCGAATCGAGGTAAATCAGGAAATGG
ATGTTTTAAAAGAATTTATAGAGCAGTCTCTTGAAATTTTAAAACCGGGCGGAAGATTCTCTGTAATCTCATACCACTCT
TTAGAAGACAGACTGGTAAAAAGATTTATTAAAAACGGAATGTTTGAAGGAGAGCCAGAGAGAGATTTTTACGGAAACTT
CTCGGTGCCGTTTAAAACAATAGGAAAACTGATTGTTCCGGATGATGAGGAAATCAAAATTAACAATAGAGCAAGAAGTG
CAAAATTGAGAATTGCCGAAAAGATATAA

Upstream 100 bases:

>100_bases
AATATTGTGGAAATTTGGGATAAAGATTTATACGAAAAATCAATAAGCGGCGAAGATATGGATTTTGCCGATTTAGCCGA
GGAAGTAATGGGAAATATTA

Downstream 100 bases:

>100_bases
TACATATATATAGGTAGAAAATGAAAAGTGGAGTATTCAGCATATTAAAAGCAAGATTTCTAATAAACGACGATGCCGTA
AAAAACTGGCGTTTCATTGT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTTTMEYHNPVLLHPTVDGLDIKPDGIYVDVTFGGGGHSKEILRRLGPNGKLFAFDQDEDALANALPDERFTLINENFRF
IKRFLRFHGIKAVDGILADLGVSSHQFDVPERGFSTRFDAELDMRMSQKNDLNAYRVVNEYEEQDLRRVFFDYGELKNAP
VLARTIVEARHHRPIKTTDELKEVLKKYLPEKVRNKILAQIYQAIRIEVNQEMDVLKEFIEQSLEILKPGGRFSVISYHS
LEDRLVKRFIKNGMFEGEPERDFYGNFSVPFKTIGKLIVPDDEEIKINNRARSAKLRIAEKI

Sequences:

>Translated_302_residues
MTTTMEYHNPVLLHPTVDGLDIKPDGIYVDVTFGGGGHSKEILRRLGPNGKLFAFDQDEDALANALPDERFTLINENFRF
IKRFLRFHGIKAVDGILADLGVSSHQFDVPERGFSTRFDAELDMRMSQKNDLNAYRVVNEYEEQDLRRVFFDYGELKNAP
VLARTIVEARHHRPIKTTDELKEVLKKYLPEKVRNKILAQIYQAIRIEVNQEMDVLKEFIEQSLEILKPGGRFSVISYHS
LEDRLVKRFIKNGMFEGEPERDFYGNFSVPFKTIGKLIVPDDEEIKINNRARSAKLRIAEKI
>Mature_301_residues
TTTMEYHNPVLLHPTVDGLDIKPDGIYVDVTFGGGGHSKEILRRLGPNGKLFAFDQDEDALANALPDERFTLINENFRFI
KRFLRFHGIKAVDGILADLGVSSHQFDVPERGFSTRFDAELDMRMSQKNDLNAYRVVNEYEEQDLRRVFFDYGELKNAPV
LARTIVEARHHRPIKTTDELKEVLKKYLPEKVRNKILAQIYQAIRIEVNQEMDVLKEFIEQSLEILKPGGRFSVISYHSL
EDRLVKRFIKNGMFEGEPERDFYGNFSVPFKTIGKLIVPDDEEIKINNRARSAKLRIAEKI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=343, Percent_Identity=33.2361516034985, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI165377202, Length=197, Percent_Identity=37.0558375634518, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1786270, Length=307, Percent_Identity=42.9967426710098, Blast_Score=215, Evalue=3e-57,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=33.6336336336336, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=33.6336336336336, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_FLAJ1 (A5FIX6)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194154.1
- ProteinModelPortal:   A5FIX6
- SMR:   A5FIX6
- STRING:   A5FIX6
- GeneID:   5091870
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1803
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   FJOH376686:FJOH_1803-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34945; Mature: 34814

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: BINDING 56-56 BINDING 84-84 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTMEYHNPVLLHPTVDGLDIKPDGIYVDVTFGGGGHSKEILRRLGPNGKLFAFDQDED
CCCCCCCCCCEEEECCCCCCEECCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCHH
ALANALPDERFTLINENFRFIKRFLRFHGIKAVDGILADLGVSSHQFDVPERGFSTRFDA
HHHHCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ELDMRMSQKNDLNAYRVVNEYEEQDLRRVFFDYGELKNAPVLARTIVEARHHRPIKTTDE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
LKEVLKKYLPEKVRNKILAQIYQAIRIEVNQEMDVLKEFIEQSLEILKPGGRFSVISYHS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHH
LEDRLVKRFIKNGMFEGEPERDFYGNFSVPFKTIGKLIVPDDEEIKINNRARSAKLRIAE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCEEECCCHHHHHHHHHH
KI
CC
>Mature Secondary Structure 
TTTMEYHNPVLLHPTVDGLDIKPDGIYVDVTFGGGGHSKEILRRLGPNGKLFAFDQDED
CCCCCCCCCEEEECCCCCCEECCCCEEEEEEECCCCCHHHHHHHHCCCCCEEEECCCHH
ALANALPDERFTLINENFRFIKRFLRFHGIKAVDGILADLGVSSHQFDVPERGFSTRFDA
HHHHCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
ELDMRMSQKNDLNAYRVVNEYEEQDLRRVFFDYGELKNAPVLARTIVEARHHRPIKTTDE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHH
LKEVLKKYLPEKVRNKILAQIYQAIRIEVNQEMDVLKEFIEQSLEILKPGGRFSVISYHS
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHH
LEDRLVKRFIKNGMFEGEPERDFYGNFSVPFKTIGKLIVPDDEEIKINNRARSAKLRIAE
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCEECCCCCCEEECCCHHHHHHHHHH
KI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA