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The map label for this gene is gidB

Identifier: 146299509

GI number: 146299509

Start: 2023774

End: 2024403

Strand: Direct

Name: gidB

Synonym: Fjoh_1749

Alternate gene names: 146299509

Gene position: 2023774-2024403 (Clockwise)

Preceding gene: 146299503

Following gene: 146299514

Centisome position: 33.19

GC content: 31.11

Gene sequence:

>630_bases
ATGGATGAGATATTGAAATATTTTCCCAATTTGACCGATCTTCAAATCGAACAATTTCAAAAATTAGACTTTTTATACCA
CAATTGGAATGAAAAAATCAATGTTATTTCACGCAAAGACATTGATTCATTATATACAAAACACATTTTACATTCTTTAG
GAATTGCTAAAGTTATGAAATTCGAACCTGGAACAACAGTTCTGGATGTTGGAACAGGAGGAGGTTTTCCTGGAATTCCG
TTAGCGATTCTTTTTCCAGAAACTCGTTTTTATTTGATTGATGTTATTGCTAAAAAAATAAAAGTGGTTCAGGGCGTTGT
AGATGCATTAGAATTAAAAAATGTAAAAGCAGAACAAAAACGTGCCGAATTGGTAAAAGGTGATTTCGATTTTATTGTGA
GCCGTGCCGTAACTAATATGCCTGATTTTGTTTCTTGGATAAAAGATAAAATTAAAAAGCAGCACAAGCATAAACTAAAA
AATGGGATTCTTTATCTTAAAGGTGGCGATTTGTCTGAAGAACTAAAAGATTTTCCTGCTGCAACCTTATATGATTTATC
AGAGATATTTGAAGATGAATTTTTTGAAACTAAAAAAGTGGTTCATCTTCCTTTAAAGTTTAAACCTTAG

Upstream 100 bases:

>100_bases
TTAAATAGGTTTCAAAGGTAATTATTATAAATTCTCAATTTGCAAAATTGAGTTAAATATTTCAATCGTAAAGTAGTACT
TTTGTTAAAAATTTACAGCA

Downstream 100 bases:

>100_bases
TTTGATATAAAAAAAACGGAAAGAAATTCTTTCCGGTTTTTTTTGAATTAATTGTAATTATTGTAAACTATTTGTTTCTG
TTAATTTTATCTCTGCGAGC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MDEILKYFPNLTDLQIEQFQKLDFLYHNWNEKINVISRKDIDSLYTKHILHSLGIAKVMKFEPGTTVLDVGTGGGFPGIP
LAILFPETRFYLIDVIAKKIKVVQGVVDALELKNVKAEQKRAELVKGDFDFIVSRAVTNMPDFVSWIKDKIKKQHKHKLK
NGILYLKGGDLSEELKDFPAATLYDLSEIFEDEFFETKKVVHLPLKFKP

Sequences:

>Translated_209_residues
MDEILKYFPNLTDLQIEQFQKLDFLYHNWNEKINVISRKDIDSLYTKHILHSLGIAKVMKFEPGTTVLDVGTGGGFPGIP
LAILFPETRFYLIDVIAKKIKVVQGVVDALELKNVKAEQKRAELVKGDFDFIVSRAVTNMPDFVSWIKDKIKKQHKHKLK
NGILYLKGGDLSEELKDFPAATLYDLSEIFEDEFFETKKVVHLPLKFKP
>Mature_209_residues
MDEILKYFPNLTDLQIEQFQKLDFLYHNWNEKINVISRKDIDSLYTKHILHSLGIAKVMKFEPGTTVLDVGTGGGFPGIP
LAILFPETRFYLIDVIAKKIKVVQGVVDALELKNVKAEQKRAELVKGDFDFIVSRAVTNMPDFVSWIKDKIKKQHKHKLK
NGILYLKGGDLSEELKDFPAATLYDLSEIFEDEFFETKKVVHLPLKFKP

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=167, Percent_Identity=33.5329341317365, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_FLAJ1 (A5FJ42)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001194100.1
- ProteinModelPortal:   A5FJ42
- SMR:   A5FJ42
- STRING:   A5FJ42
- GeneID:   5093091
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_1749
- eggNOG:   COG0357
- HOGENOM:   HBG308018
- OMA:   ICLKGGE
- ProtClustDB:   PRK00107
- BioCyc:   FJOH376686:FJOH_1749-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.- [C]

Molecular weight: Translated: 24039; Mature: 24039

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEILKYFPNLTDLQIEQFQKLDFLYHNWNEKINVISRKDIDSLYTKHILHSLGIAKVMK
CCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHE
FEPGTTVLDVGTGGGFPGIPLAILFPETRFYLIDVIAKKIKVVQGVVDALELKNVKAEQK
ECCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RAELVKGDFDFIVSRAVTNMPDFVSWIKDKIKKQHKHKLKNGILYLKGGDLSEELKDFPA
HHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCC
ATLYDLSEIFEDEFFETKKVVHLPLKFKP
HHHHHHHHHHHHHHHHCHHEEEECCEECC
>Mature Secondary Structure
MDEILKYFPNLTDLQIEQFQKLDFLYHNWNEKINVISRKDIDSLYTKHILHSLGIAKVMK
CCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHE
FEPGTTVLDVGTGGGFPGIPLAILFPETRFYLIDVIAKKIKVVQGVVDALELKNVKAEQK
ECCCCEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RAELVKGDFDFIVSRAVTNMPDFVSWIKDKIKKQHKHKLKNGILYLKGGDLSEELKDFPA
HHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHCCC
ATLYDLSEIFEDEFFETKKVVHLPLKFKP
HHHHHHHHHHHHHHHHCHHEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA