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The map label for this gene is aceA [H]

Identifier: 146299499

GI number: 146299499

Start: 2012889

End: 2014169

Strand: Direct

Name: aceA [H]

Synonym: Fjoh_1739

Alternate gene names: 146299499

Gene position: 2012889-2014169 (Clockwise)

Preceding gene: 146299498

Following gene: 146299503

Centisome position: 33.02

GC content: 39.66

Gene sequence:

>1281_bases
ATGAAAACAACAGAAGACAGAATTCAGGAATTGATTAACGATTGGATTACGAACCCGAGATGGAAAGGAGTTGAGCGTCC
TTACACTGCAAGCGAAGTAGTAACACTTCAGGGGTCTTATAAAATTGAGCATTCTATTGCCAAAATGGGTGCTGAAAAAT
TATGGAGAAAGTTAAAAAGTCAGGATTATGTTGCGGGTTTGGGAGCATTAACTGGAAATCAGGCGATTCAGGAAGTCGAT
GCGGGTTTAGAAGCAATTTACTTAAGTGGATGGCAGGTTGCTGCTGATGCAAATCTGGCGGGAGAAATGTATCCTGACCA
ATCGCTTTACCCGGTAAACAGCGTTCCGATGGTGGTAAAAAAGATCAACAACGCTTTATTGCGTGCTGATCAGATTCAGA
CTGTAAATAATATTGAAGATAGAAAAGATTATTTAGTTCCAATTGTAGCTGATGCCGAAGCTGGTTTTGGCGGCAACTTA
AATGCTTTCGAATTAATGAAATCGATGATTGAAGCTGGAGCTTCTGGAGTTCATTTTGAAGATCAGCTGAGTTCTGCTAA
AAAGTGCGGACATTTGGGCGGGAAGGTTTTAGTTCCAACTCAGGAAGCAATAAATAAATTAATTGCGGCACGATTGGCAT
CAGATGTTATGGGCGTTTCAACTTTAATAATTGCTCGAACAGATGCCGATGCAGCTAATTTACTAACAAGCGATGCGGAT
CCAAGAGATAGAAAGTTTTTAACAGGTGAAAAAACGACTGAAGGTTTCTTCTACGTTAAAAACGGAATTGATCAGGGAAT
TGCAAGAGGTTTAAGTTACGCACCATACGCTGATTTAATCTGGATGGAAACCAGTAATCCCGATTTAGATTATGCAAGAA
AGTTTGCAAAAGCAATGAAAAAAGAATTTCCAGACAAAATGCTGGCGTACAATTGTTCGCCGTCTTTCAACTGGGCGGCA
AAATTGACGGTTGCTGAAATGGAAACGTTTAGAGAAGATTTAGCAGCAATGGGATATAGTTTTCAGTTCATTACTTTAGC
AGGATTCCATGCTTTGAATACCAGTATGTTTGAATTGTCTAAAGCTTACAAAGAGAGAGGAATGGCAGGCTATTCTGAAT
TGCAGGAACGCGAATTTGCTTTGCAGAAAAACGGATTCAGAGCTGTAAAACATCAGGCTTTTGTGGGAACTTCTTATTTT
GATGCCGTTCAAAATACGGTAATGTTAGGGAAATCAGCAATAACCGCAATGGAACATTCAACAGAGGTTGAACAGTTTTA
A

Upstream 100 bases:

>100_bases
ATAACTATGTTTCTATGTGTTTAAAAATCTTGGTTCAAACTGAAAACTGAAAACCGAGACTGAAAACTTTATTAACGAAC
CACTTTAACTAAACTATATT

Downstream 100 bases:

>100_bases
TTTTTTTTACCATTAAGATATTAAGAAAGTTAAGCTTTATATCCTTAAAAAAGAAGGAAGATTAAGTCTAGCTTAATGAA
TCTTAATATCTTAATGGTTT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKTTEDRIQELINDWITNPRWKGVERPYTASEVVTLQGSYKIEHSIAKMGAEKLWRKLKSQDYVAGLGALTGNQAIQEVD
AGLEAIYLSGWQVAADANLAGEMYPDQSLYPVNSVPMVVKKINNALLRADQIQTVNNIEDRKDYLVPIVADAEAGFGGNL
NAFELMKSMIEAGASGVHFEDQLSSAKKCGHLGGKVLVPTQEAINKLIAARLASDVMGVSTLIIARTDADAANLLTSDAD
PRDRKFLTGEKTTEGFFYVKNGIDQGIARGLSYAPYADLIWMETSNPDLDYARKFAKAMKKEFPDKMLAYNCSPSFNWAA
KLTVAEMETFREDLAAMGYSFQFITLAGFHALNTSMFELSKAYKERGMAGYSELQEREFALQKNGFRAVKHQAFVGTSYF
DAVQNTVMLGKSAITAMEHSTEVEQF

Sequences:

>Translated_426_residues
MKTTEDRIQELINDWITNPRWKGVERPYTASEVVTLQGSYKIEHSIAKMGAEKLWRKLKSQDYVAGLGALTGNQAIQEVD
AGLEAIYLSGWQVAADANLAGEMYPDQSLYPVNSVPMVVKKINNALLRADQIQTVNNIEDRKDYLVPIVADAEAGFGGNL
NAFELMKSMIEAGASGVHFEDQLSSAKKCGHLGGKVLVPTQEAINKLIAARLASDVMGVSTLIIARTDADAANLLTSDAD
PRDRKFLTGEKTTEGFFYVKNGIDQGIARGLSYAPYADLIWMETSNPDLDYARKFAKAMKKEFPDKMLAYNCSPSFNWAA
KLTVAEMETFREDLAAMGYSFQFITLAGFHALNTSMFELSKAYKERGMAGYSELQEREFALQKNGFRAVKHQAFVGTSYF
DAVQNTVMLGKSAITAMEHSTEVEQF
>Mature_426_residues
MKTTEDRIQELINDWITNPRWKGVERPYTASEVVTLQGSYKIEHSIAKMGAEKLWRKLKSQDYVAGLGALTGNQAIQEVD
AGLEAIYLSGWQVAADANLAGEMYPDQSLYPVNSVPMVVKKINNALLRADQIQTVNNIEDRKDYLVPIVADAEAGFGGNL
NAFELMKSMIEAGASGVHFEDQLSSAKKCGHLGGKVLVPTQEAINKLIAARLASDVMGVSTLIIARTDADAANLLTSDAD
PRDRKFLTGEKTTEGFFYVKNGIDQGIARGLSYAPYADLIWMETSNPDLDYARKFAKAMKKEFPDKMLAYNCSPSFNWAA
KLTVAEMETFREDLAAMGYSFQFITLAGFHALNTSMFELSKAYKERGMAGYSELQEREFALQKNGFRAVKHQAFVGTSYF
DAVQNTVMLGKSAITAMEHSTEVEQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=435, Percent_Identity=60.2298850574713, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1786525, Length=303, Percent_Identity=23.7623762376238, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=414, Percent_Identity=58.4541062801932, Blast_Score=495, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17561814, Length=414, Percent_Identity=58.4541062801932, Blast_Score=495, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320908, Length=254, Percent_Identity=36.6141732283465, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325263, Length=261, Percent_Identity=36.3984674329502, Blast_Score=154, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47115; Mature: 47115

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTEDRIQELINDWITNPRWKGVERPYTASEVVTLQGSYKIEHSIAKMGAEKLWRKLKS
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
QDYVAGLGALTGNQAIQEVDAGLEAIYLSGWQVAADANLAGEMYPDQSLYPVNSVPMVVK
CCHHHHHHHHHHHHHHHHHHCCHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
KINNALLRADQIQTVNNIEDRKDYLVPIVADAEAGFGGNLNAFELMKSMIEAGASGVHFE
HHHHHHHHHHHHHHHHCHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHH
DQLSSAKKCGHLGGKVLVPTQEAINKLIAARLASDVMGVSTLIIARTDADAANLLTSDAD
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHCCCCC
PRDRKFLTGEKTTEGFFYVKNGIDQGIARGLSYAPYADLIWMETSNPDLDYARKFAKAMK
CCCCCEECCCCCCCCEEEECCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
KEFPDKMLAYNCSPSFNWAAKLTVAEMETFREDLAAMGYSFQFITLAGFHALNTSMFELS
HHCCHHHEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
KAYKERGMAGYSELQEREFALQKNGFRAVKHQAFVGTSYFDAVQNTVMLGKSAITAMEHS
HHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
TEVEQF
HHHCCC
>Mature Secondary Structure
MKTTEDRIQELINDWITNPRWKGVERPYTASEVVTLQGSYKIEHSIAKMGAEKLWRKLKS
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCC
QDYVAGLGALTGNQAIQEVDAGLEAIYLSGWQVAADANLAGEMYPDQSLYPVNSVPMVVK
CCHHHHHHHHHHHHHHHHHHCCHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHH
KINNALLRADQIQTVNNIEDRKDYLVPIVADAEAGFGGNLNAFELMKSMIEAGASGVHFE
HHHHHHHHHHHHHHHHCHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCHH
DQLSSAKKCGHLGGKVLVPTQEAINKLIAARLASDVMGVSTLIIARTDADAANLLTSDAD
HHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHCCCCC
PRDRKFLTGEKTTEGFFYVKNGIDQGIARGLSYAPYADLIWMETSNPDLDYARKFAKAMK
CCCCCEECCCCCCCCEEEECCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHH
KEFPDKMLAYNCSPSFNWAAKLTVAEMETFREDLAAMGYSFQFITLAGFHALNTSMFELS
HHCCHHHEEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
KAYKERGMAGYSELQEREFALQKNGFRAVKHQAFVGTSYFDAVQNTVMLGKSAITAMEHS
HHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
TEVEQF
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]