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The map label for this gene is mls [H]

Identifier: 146299498

GI number: 146299498

Start: 2011042

End: 2012664

Strand: Direct

Name: mls [H]

Synonym: Fjoh_1738

Alternate gene names: 146299498

Gene position: 2011042-2012664 (Clockwise)

Preceding gene: 146299492

Following gene: 146299499

Centisome position: 32.98

GC content: 36.48

Gene sequence:

>1623_bases
ATGAAATTAAAAACCACAGCTATGAAAAACCAATTAGAAATTACCGAAACGGCAATGGAATTTTTAGCCGAAAAGAAGCT
TCGTTATCCAAAGATCTGGACAGAAGAAGCCATTGTTTTTATAACTGAATTGCATAGAAAATTCGAATCACAGCGAAAAC
TATTGCTTTTACAGCGCGAACAGAAACAAGTCACTTTTGATCAGGGAATTATGCCTGTTTTTATTCCAGAAACCAAAAGT
GTGAGAGAAGGCAGTTGGAAAGCTGGAGAAATTCCGAAAGATTTATTGGATCGAAGAGTAGAAATTACCGGGCCGGTTGA
CCGCAAAATGATTATTAATGCTTTGAATTCTGGCGCCAAAACATTTATGGCCGATTTTGAAGACAGCACTTCGCCAACCT
GGCAAAACTTGATGGACGGACAGGTAAATTTGATTGATGCGGTAAACAAAACCATTACGTTTACTGATTTGGTAAAACAG
AAATCGTATCATTTAAATGAAAAAATCGCAACGTTAATTGTACGTCCGAGAGGTTTGCATTTGCCAGAAAAACATGTTTT
AGTTGAAGGAAATGAAGTTTCGGGTTCTTTGATAGATTTTGGTTTGTATGTTTTTCACAATCATAAACGACTTTTAGAAA
ACAATTCTGGACCGTATTTCTACATTCCGAAATTAGAGCATTATCTGGAAGCGCGCTGGTGGAATAATGTAATTGATTTT
ACCGAGGATTATTTAAAACTGAACAGAGGAACGATAAAAGTGACGGTTTTAATTGAAACGATAACGGCAAGTTTTCAATT
GGATGAAATTATCTACGAATTGAAAGAACATATCGTTGGCTTAAACTGCGGACGCTGGGATTATATTTTCTCCTACATCA
AAAAGTTTAGAAAAAATCCAAAATTCATCGTGCCAGATCGGGATCAGGTAACTATGACTTCGCCTTTTATGAGTGCTTAT
TCAAATCTGGTAATTCAAAGATGTCATAAAAGAGGAATTCATGCAATTGGCGGAATGGCTGCACAGATTCCGATTCGAAA
TAACGAAGAAGCTAATGCAGTTGCTTTCGCAAAAGTAAGAACCGATAAGGAACGTGAAGTTAAAAATGGACACGACGGAA
CTTGGGTAGCGCATCCGGATTTAGTTGCAATAGCAAAAGAAATTTTTGATAAAGGAATGCCAACTCCAAATCAGATTCAC
GTAAAAAGAGAACATCGAAAAATTACAGAAGCCGATTTGATTGAACCGCCAATTGGAATCATTACTGAAAATGGTGTCCG
CAAAAACATCAACGTTGGAGTTTTATATCTGGCTTCATGGCTAAACGGACAAGGCGCCGCCGCTTTACATAATTTGATGG
AAGATGCTGCAACGGCCGAAATTTCGAGATCACAATTGTGGCAATGGCTTCAGAATAAAGTGATTTTAGATAATGGACGA
AAATTAGATTTGGCTTATTATCATGAATTGGCTTTAAATGAATTCAGAAAAATCAAGGAAGAAATAGGAGAAGAAAATTA
TGAAAAACAACAATTTCCATTAGCCGAAAAAATGCTGGAAAGACTGGTTGTAAACCTGCATTTTGTGGATTTCGTTACTC
TTCCATGTTATAAATATCTATAA

Upstream 100 bases:

>100_bases
AAAAAACTTTTTTATAATATTGTGGTGTAGAAAATATTAATTATCAGTTTGTTATGGATTTGTGTTTTTGAAAAACATAG
AATTGATTCAATTGCTGCTA

Downstream 100 bases:

>100_bases
TTTATTTAAACACATAGAAACATAGATTTTATTTTTTCTATAAAAAGAGAAAAGAAAAAACTGGTTTTTCATGCATAGAT
AACTATGTGAATTGAAACAA

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MKLKTTAMKNQLEITETAMEFLAEKKLRYPKIWTEEAIVFITELHRKFESQRKLLLLQREQKQVTFDQGIMPVFIPETKS
VREGSWKAGEIPKDLLDRRVEITGPVDRKMIINALNSGAKTFMADFEDSTSPTWQNLMDGQVNLIDAVNKTITFTDLVKQ
KSYHLNEKIATLIVRPRGLHLPEKHVLVEGNEVSGSLIDFGLYVFHNHKRLLENNSGPYFYIPKLEHYLEARWWNNVIDF
TEDYLKLNRGTIKVTVLIETITASFQLDEIIYELKEHIVGLNCGRWDYIFSYIKKFRKNPKFIVPDRDQVTMTSPFMSAY
SNLVIQRCHKRGIHAIGGMAAQIPIRNNEEANAVAFAKVRTDKEREVKNGHDGTWVAHPDLVAIAKEIFDKGMPTPNQIH
VKREHRKITEADLIEPPIGIITENGVRKNINVGVLYLASWLNGQGAAALHNLMEDAATAEISRSQLWQWLQNKVILDNGR
KLDLAYYHELALNEFRKIKEEIGEENYEKQQFPLAEKMLERLVVNLHFVDFVTLPCYKYL

Sequences:

>Translated_540_residues
MKLKTTAMKNQLEITETAMEFLAEKKLRYPKIWTEEAIVFITELHRKFESQRKLLLLQREQKQVTFDQGIMPVFIPETKS
VREGSWKAGEIPKDLLDRRVEITGPVDRKMIINALNSGAKTFMADFEDSTSPTWQNLMDGQVNLIDAVNKTITFTDLVKQ
KSYHLNEKIATLIVRPRGLHLPEKHVLVEGNEVSGSLIDFGLYVFHNHKRLLENNSGPYFYIPKLEHYLEARWWNNVIDF
TEDYLKLNRGTIKVTVLIETITASFQLDEIIYELKEHIVGLNCGRWDYIFSYIKKFRKNPKFIVPDRDQVTMTSPFMSAY
SNLVIQRCHKRGIHAIGGMAAQIPIRNNEEANAVAFAKVRTDKEREVKNGHDGTWVAHPDLVAIAKEIFDKGMPTPNQIH
VKREHRKITEADLIEPPIGIITENGVRKNINVGVLYLASWLNGQGAAALHNLMEDAATAEISRSQLWQWLQNKVILDNGR
KLDLAYYHELALNEFRKIKEEIGEENYEKQQFPLAEKMLERLVVNLHFVDFVTLPCYKYL
>Mature_540_residues
MKLKTTAMKNQLEITETAMEFLAEKKLRYPKIWTEEAIVFITELHRKFESQRKLLLLQREQKQVTFDQGIMPVFIPETKS
VREGSWKAGEIPKDLLDRRVEITGPVDRKMIINALNSGAKTFMADFEDSTSPTWQNLMDGQVNLIDAVNKTITFTDLVKQ
KSYHLNEKIATLIVRPRGLHLPEKHVLVEGNEVSGSLIDFGLYVFHNHKRLLENNSGPYFYIPKLEHYLEARWWNNVIDF
TEDYLKLNRGTIKVTVLIETITASFQLDEIIYELKEHIVGLNCGRWDYIFSYIKKFRKNPKFIVPDRDQVTMTSPFMSAY
SNLVIQRCHKRGIHAIGGMAAQIPIRNNEEANAVAFAKVRTDKEREVKNGHDGTWVAHPDLVAIAKEIFDKGMPTPNQIH
VKREHRKITEADLIEPPIGIITENGVRKNINVGVLYLASWLNGQGAAALHNLMEDAATAEISRSQLWQWLQNKVILDNGR
KLDLAYYHELALNEFRKIKEEIGEENYEKQQFPLAEKMLERLVVNLHFVDFVTLPCYKYL

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=512, Percent_Identity=50.390625, Blast_Score=529, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17561814, Length=520, Percent_Identity=53.6538461538462, Blast_Score=548, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71982926, Length=404, Percent_Identity=58.1683168316832, Blast_Score=476, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6324212, Length=516, Percent_Identity=47.8682170542636, Blast_Score=483, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322222, Length=517, Percent_Identity=47.5822050290135, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 62473; Mature: 62473

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTTAMKNQLEITETAMEFLAEKKLRYPKIWTEEAIVFITELHRKFESQRKLLLLQRE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHEEHH
QKQVTFDQGIMPVFIPETKSVREGSWKAGEIPKDLLDRRVEITGPVDRKMIINALNSGAK
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHCCCHH
TFMADFEDSTSPTWQNLMDGQVNLIDAVNKTITFTDLVKQKSYHLNEKIATLIVRPRGLH
HHHHCCCCCCCCHHHHHCCCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHEEEECCCCCC
LPEKHVLVEGNEVSGSLIDFGLYVFHNHKRLLENNSGPYFYIPKLEHYLEARWWNNVIDF
CCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
TEDYLKLNRGTIKVTVLIETITASFQLDEIIYELKEHIVGLNCGRWDYIFSYIKKFRKNP
HHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
KFIVPDRDQVTMTSPFMSAYSNLVIQRCHKRGIHAIGGMAAQIPIRNNEEANAVAFAKVR
CEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCEEEEEEEC
TDKEREVKNGHDGTWVAHPDLVAIAKEIFDKGMPTPNQIHVKREHRKITEADLIEPPIGI
CCHHHHHCCCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHCCCCCCCE
ITENGVRKNINVGVLYLASWLNGQGAAALHNLMEDAATAEISRSQLWQWLQNKVILDNGR
EECCCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCC
KLDLAYYHELALNEFRKIKEEIGEENYEKQQFPLAEKMLERLVVNLHFVDFVTLPCYKYL
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MKLKTTAMKNQLEITETAMEFLAEKKLRYPKIWTEEAIVFITELHRKFESQRKLLLLQRE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHEEHH
QKQVTFDQGIMPVFIPETKSVREGSWKAGEIPKDLLDRRVEITGPVDRKMIINALNSGAK
HHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHCCCHH
TFMADFEDSTSPTWQNLMDGQVNLIDAVNKTITFTDLVKQKSYHLNEKIATLIVRPRGLH
HHHHCCCCCCCCHHHHHCCCCCHHHHHHCCCEEHHHHHHHHHHHHHHHHHEEEECCCCCC
LPEKHVLVEGNEVSGSLIDFGLYVFHNHKRLLENNSGPYFYIPKLEHYLEARWWNNVIDF
CCCCEEEEECCCCCCHHHHHHHHHHCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
TEDYLKLNRGTIKVTVLIETITASFQLDEIIYELKEHIVGLNCGRWDYIFSYIKKFRKNP
HHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCC
KFIVPDRDQVTMTSPFMSAYSNLVIQRCHKRGIHAIGGMAAQIPIRNNEEANAVAFAKVR
CEECCCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCCCCCEEEEEEEC
TDKEREVKNGHDGTWVAHPDLVAIAKEIFDKGMPTPNQIHVKREHRKITEADLIEPPIGI
CCHHHHHCCCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEEHHHCCCCHHHCCCCCCCE
ITENGVRKNINVGVLYLASWLNGQGAAALHNLMEDAATAEISRSQLWQWLQNKVILDNGR
EECCCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCC
KLDLAYYHELALNEFRKIKEEIGEENYEKQQFPLAEKMLERLVVNLHFVDFVTLPCYKYL
EEEHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA