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The map label for this gene is dctA2 [H]

Identifier: 146299273

GI number: 146299273

Start: 1743972

End: 1745195

Strand: Direct

Name: dctA2 [H]

Synonym: Fjoh_1513

Alternate gene names: 146299273

Gene position: 1743972-1745195 (Clockwise)

Preceding gene: 146299272

Following gene: 146299274

Centisome position: 28.6

GC content: 36.44

Gene sequence:

>1224_bases
ATGGAAGTTAAGAAAATCAATTTTTTACAGAGTTACAGCAGCATTTTATGGCTTCTTGGCGGAATTATAATTGGAAGTAT
TTTTGGATTGGTATTTGGAGAAGATGTTTTGATTATAAAACCTCTTGGCGATATATTCCTGAATTTACTTTTTACAGCCA
TTATTCCGCTTATATTTTTCACAATAGGTTCTTCGGTTGCGAATCTGGAGCGAACAGAAAAGCTAGGAAAACTGTTTGTA
ATAATGGTATTGGTTTTTCTCGCGACAATTCTGATTTCAGCAATTGTAATGATTTGTGCTGTTTTTCTTTTTCCAATTCA
TGAACATATTGCAATTGCCAAAGTTGTTTTTGAAGAAACAGCATCAGGATCGGCTGGAGATCAAATTGCAAAACTGCTTA
CAGCCAATGATTTCTTCGAACTATTGTCTCGAAAAAGCATGCTGGCATTGATTATTTTCTCTTTTTTAGTAGGTTTTGCA
ACATTACAATCGGGAGAAAAAGGAAATGCTTTCAAAAGTTTTCTAGATTCAGGAAACGAGGTAATGAAACAGCTTTTGAA
CATTATAATGAAATTTGCACCAATTGGTTTAGGAGCTTATTTTGCTTACCAAGTTGGTGTTTTCGGACCACAATTGTTAG
GCGTTTATGCAAAACCAATGGCAGTTTATTATGCTGCCTGTATTTTCTATTTTTTTGTGTTCTTTAGTTTATATGCTTTT
GCTGCTGGCGGAAAACGAGCTTTTAAAGTTTTTTGGAGCAATAACATAATGCCTTCTTTAACAGCAGTTGGAACCTGCAG
CAGCATTGCAACGATTCCGGCCAATTTAACTGCGGCAGAAAAAATGGGAATTCCGGCACATGTACGAAATCTGGTAATTC
CGCTTGGCGCGCCTCTGCATAAAGACGGTTCGAGTATGTCATCCATCTTAAAAATAACTTTTTTGTTTGCCATGTTTGGT
AAAGATTTTTCAGATCCGATGACAATTCTTTTAGCACTTGGAATTACGGTTATAGTTTCAATTGTCGAAGGCGGAATCCC
TAATGGAGGTTATATTGGCGAAATCTTAGCCATAACTGTTTATGGTTTTCCAATGGAACAAGCACTTCCGGTTGCCATGA
TTCTGGGAACTTTAGTAGATCCAATCGCTACTTTATTAAATGCCAATGGAGACGTAATCTGTTCTATGATGGTTTCAAGA
TTCTCTGAAAAGACGAAATGGTAG

Upstream 100 bases:

>100_bases
ATGTTTGTTTAAATAAGTCAAACGCCTTTATAGACAAAGAGAACTATGTTTCTATGTGTTAAAAAATAAAGGTTGCTGAA
TTCTAAACCCTTGTATCATT

Downstream 100 bases:

>100_bases
CTTCTCTATAAAATTTCAATACTTAAAATAAATACTAAAAACAACTTATCTCGTGTGAAATGGCTTTGACTTCGCTCAGC
CTGACATTGCGTGATGTAAA

Product: sodium:dicarboxylate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MEVKKINFLQSYSSILWLLGGIIIGSIFGLVFGEDVLIIKPLGDIFLNLLFTAIIPLIFFTIGSSVANLERTEKLGKLFV
IMVLVFLATILISAIVMICAVFLFPIHEHIAIAKVVFEETASGSAGDQIAKLLTANDFFELLSRKSMLALIIFSFLVGFA
TLQSGEKGNAFKSFLDSGNEVMKQLLNIIMKFAPIGLGAYFAYQVGVFGPQLLGVYAKPMAVYYAACIFYFFVFFSLYAF
AAGGKRAFKVFWSNNIMPSLTAVGTCSSIATIPANLTAAEKMGIPAHVRNLVIPLGAPLHKDGSSMSSILKITFLFAMFG
KDFSDPMTILLALGITVIVSIVEGGIPNGGYIGEILAITVYGFPMEQALPVAMILGTLVDPIATLLNANGDVICSMMVSR
FSEKTKW

Sequences:

>Translated_407_residues
MEVKKINFLQSYSSILWLLGGIIIGSIFGLVFGEDVLIIKPLGDIFLNLLFTAIIPLIFFTIGSSVANLERTEKLGKLFV
IMVLVFLATILISAIVMICAVFLFPIHEHIAIAKVVFEETASGSAGDQIAKLLTANDFFELLSRKSMLALIIFSFLVGFA
TLQSGEKGNAFKSFLDSGNEVMKQLLNIIMKFAPIGLGAYFAYQVGVFGPQLLGVYAKPMAVYYAACIFYFFVFFSLYAF
AAGGKRAFKVFWSNNIMPSLTAVGTCSSIATIPANLTAAEKMGIPAHVRNLVIPLGAPLHKDGSSMSSILKITFLFAMFG
KDFSDPMTILLALGITVIVSIVEGGIPNGGYIGEILAITVYGFPMEQALPVAMILGTLVDPIATLLNANGDVICSMMVSR
FSEKTKW
>Mature_407_residues
MEVKKINFLQSYSSILWLLGGIIIGSIFGLVFGEDVLIIKPLGDIFLNLLFTAIIPLIFFTIGSSVANLERTEKLGKLFV
IMVLVFLATILISAIVMICAVFLFPIHEHIAIAKVVFEETASGSAGDQIAKLLTANDFFELLSRKSMLALIIFSFLVGFA
TLQSGEKGNAFKSFLDSGNEVMKQLLNIIMKFAPIGLGAYFAYQVGVFGPQLLGVYAKPMAVYYAACIFYFFVFFSLYAF
AAGGKRAFKVFWSNNIMPSLTAVGTCSSIATIPANLTAAEKMGIPAHVRNLVIPLGAPLHKDGSSMSSILKITFLFAMFG
KDFSDPMTILLALGITVIVSIVEGGIPNGGYIGEILAITVYGFPMEQALPVAMILGTLVDPIATLLNANGDVICSMMVSR
FSEKTKW

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI223468564, Length=268, Percent_Identity=24.6268656716418, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI169790839, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI223468566, Length=268, Percent_Identity=24.6268656716418, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI5032093, Length=270, Percent_Identity=24.8148148148148, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI4827012, Length=262, Percent_Identity=24.0458015267176, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI66773030, Length=268, Percent_Identity=25, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI262359914, Length=199, Percent_Identity=29.1457286432161, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI21314632, Length=296, Percent_Identity=22.972972972973, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI40254478, Length=251, Percent_Identity=25.4980079681275, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789947, Length=403, Percent_Identity=25.3101736972705, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790514, Length=424, Percent_Identity=22.877358490566, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788024, Length=415, Percent_Identity=23.3734939759036, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71996953, Length=265, Percent_Identity=26.4150943396226, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193206654, Length=405, Percent_Identity=22.4691358024691, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983106, Length=423, Percent_Identity=21.2765957446809, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71983099, Length=423, Percent_Identity=21.2765957446809, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=428, Percent_Identity=22.4299065420561, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583025, Length=431, Percent_Identity=22.2737819025522, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17137668, Length=431, Percent_Identity=22.2737819025522, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24583023, Length=431, Percent_Identity=22.2737819025522, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44014; Mature: 44014

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]