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The map label for this gene is metH [H]

Identifier: 146299268

GI number: 146299268

Start: 1737381

End: 1738385

Strand: Direct

Name: metH [H]

Synonym: Fjoh_1508

Alternate gene names: 146299268

Gene position: 1737381-1738385 (Clockwise)

Preceding gene: 146299267

Following gene: 146299269

Centisome position: 28.5

GC content: 39.6

Gene sequence:

>1005_bases
ATGGCAATAACAATTCAAGAAGCAATAAAAAAAAATATCCTAATCCTAGATGGAGCAATGGGAACAATGTTGCAGCGCTA
TAATTTCTCCGAAGAAGATTTCAGAGGAGAGCGTTTCAAAGATTTTCCGCATCCGTTAAAAGGAAACAACGATTTACTAT
CCCTAACACAACCACAAGCAATTCGCGATGTTCATGCCGCTTATTATGAAGCTGGTGCTGACATTGTAGAAACCAATACC
TTCTCAGGAACGACAATCGGTATGGCTGATTATCACATGGAAGATTTGGTTTACGAGTTAAACTACGAATCGGCAAAAAT
CGCAAGACAAGTAGCCGATGAGTTTACCGCAAAAAATCCAGAAAAACCACGTTTCGTTGCCGGTTCAATCGGACCGACAA
ACCGTACGGCAAGTATGTCGCCAGACGTAAATGATCCGGGTTACAGAGCTGTAACGTTTGACGATTTAAGAATTGCGTAC
AAACAACAAGCAGAAGCTTTAATGGATGGTGGATGCGATTTACTTTTAGTAGAAACAATTTTCGATACGCTAAATGCAAA
AGCAGCACTTTTTGCAATCGAAGAAGTAAAAGAAGAACGCAACATTGATATTCCAATCATGGTTTCAGGAACAATTACAG
ATGCTTCTGGAAGAACACTTTCTGGGCAGACTGTTGAAGCGTTTTTGATTTCAGTTTCACATATTCCGTTGTTAAGTGTA
GGATTCAATTGCGCTCTTGGAGCTGATTTATTGAAACCGTATTTAAAAACATTAGCGCACAATACAAGTTTTAATGTTTC
GGCACATCCAAATGCAGGATTGCCAAATGCTTTCGGACAATACGATGAAACGCCAGAACAGACTCAGGTATTCATTAAAG
AGTATTTAGAAGATAATTTAATCAATATCATCGGAGGTTGTTGCGGAACAACGCCAGATCATATTCGATTAATGGCTGAG
GTGGCGAAGGATTATAAGCCGAGAATTGCGCCAGTTATTGCGTAA

Upstream 100 bases:

>100_bases
AAGCAAAGCTAACAGGTTTGCTTCAAAATCATAATAAAAAAACTTAGCATCTTAGTCCCGATAGCTATCGGGATAATCTC
AGAAGCTAAAAAAATAAGCC

Downstream 100 bases:

>100_bases
TATAATTTCCATTCGTTGCGTGGACGGATTAAAATCCATCCCTACAAAATAATTTGAGCTGAAGGCTCTTTAGAGAGTTC
CGAAGGAACGATTCATTTTG

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAITIQEAIKKNILILDGAMGTMLQRYNFSEEDFRGERFKDFPHPLKGNNDLLSLTQPQAIRDVHAAYYEAGADIVETNT
FSGTTIGMADYHMEDLVYELNYESAKIARQVADEFTAKNPEKPRFVAGSIGPTNRTASMSPDVNDPGYRAVTFDDLRIAY
KQQAEALMDGGCDLLLVETIFDTLNAKAALFAIEEVKEERNIDIPIMVSGTITDASGRTLSGQTVEAFLISVSHIPLLSV
GFNCALGADLLKPYLKTLAHNTSFNVSAHPNAGLPNAFGQYDETPEQTQVFIKEYLEDNLINIIGGCCGTTPDHIRLMAE
VAKDYKPRIAPVIA

Sequences:

>Translated_334_residues
MAITIQEAIKKNILILDGAMGTMLQRYNFSEEDFRGERFKDFPHPLKGNNDLLSLTQPQAIRDVHAAYYEAGADIVETNT
FSGTTIGMADYHMEDLVYELNYESAKIARQVADEFTAKNPEKPRFVAGSIGPTNRTASMSPDVNDPGYRAVTFDDLRIAY
KQQAEALMDGGCDLLLVETIFDTLNAKAALFAIEEVKEERNIDIPIMVSGTITDASGRTLSGQTVEAFLISVSHIPLLSV
GFNCALGADLLKPYLKTLAHNTSFNVSAHPNAGLPNAFGQYDETPEQTQVFIKEYLEDNLINIIGGCCGTTPDHIRLMAE
VAKDYKPRIAPVIA
>Mature_333_residues
AITIQEAIKKNILILDGAMGTMLQRYNFSEEDFRGERFKDFPHPLKGNNDLLSLTQPQAIRDVHAAYYEAGADIVETNTF
SGTTIGMADYHMEDLVYELNYESAKIARQVADEFTAKNPEKPRFVAGSIGPTNRTASMSPDVNDPGYRAVTFDDLRIAYK
QQAEALMDGGCDLLLVETIFDTLNAKAALFAIEEVKEERNIDIPIMVSGTITDASGRTLSGQTVEAFLISVSHIPLLSVG
FNCALGADLLKPYLKTLAHNTSFNVSAHPNAGLPNAFGQYDETPEQTQVFIKEYLEDNLINIIGGCCGTTPDHIRLMAEV
AKDYKPRIAPVIA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=330, Percent_Identity=55.4545454545455, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1790450, Length=329, Percent_Identity=56.838905775076, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786456, Length=315, Percent_Identity=25.3968253968254, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535405, Length=328, Percent_Identity=53.9634146341463, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 36705; Mature: 36574

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITIQEAIKKNILILDGAMGTMLQRYNFSEEDFRGERFKDFPHPLKGNNDLLSLTQPQA
CEEEHHHHHHCCEEEEECHHHHHHHHCCCCHHHCCCCHHHCCCCCCCCCCCEEECCCCHH
IRDVHAAYYEAGADIVETNTFSGTTIGMADYHMEDLVYELNYESAKIARQVADEFTAKNP
HHHHHHHHHHCCCCEEECCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
EKPRFVAGSIGPTNRTASMSPDVNDPGYRAVTFDDLRIAYKQQAEALMDGGCDLLLVETI
CCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHH
FDTLNAKAALFAIEEVKEERNIDIPIMVSGTITDASGRTLSGQTVEAFLISVSHIPLLSV
HHHHCCHHHHHHHHHHHHHCCCCCEEEEECEEECCCCCEECCCHHHHHHHHHHCCCHHHC
GFNCALGADLLKPYLKTLAHNTSFNVSAHPNAGLPNAFGQYDETPEQTQVFIKEYLEDNL
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
INIIGGCCGTTPDHIRLMAEVAKDYKPRIAPVIA
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AITIQEAIKKNILILDGAMGTMLQRYNFSEEDFRGERFKDFPHPLKGNNDLLSLTQPQA
EEEHHHHHHCCEEEEECHHHHHHHHCCCCHHHCCCCHHHCCCCCCCCCCCEEECCCCHH
IRDVHAAYYEAGADIVETNTFSGTTIGMADYHMEDLVYELNYESAKIARQVADEFTAKNP
HHHHHHHHHHCCCCEEECCCCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
EKPRFVAGSIGPTNRTASMSPDVNDPGYRAVTFDDLRIAYKQQAEALMDGGCDLLLVETI
CCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEHHHH
FDTLNAKAALFAIEEVKEERNIDIPIMVSGTITDASGRTLSGQTVEAFLISVSHIPLLSV
HHHHCCHHHHHHHHHHHHHCCCCCEEEEECEEECCCCCEECCCHHHHHHHHHHCCCHHHC
GFNCALGADLLKPYLKTLAHNTSFNVSAHPNAGLPNAFGQYDETPEQTQVFIKEYLEDNL
CCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
INIIGGCCGTTPDHIRLMAEVAKDYKPRIAPVIA
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]