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The map label for this gene is cysNC [H]

Identifier: 146299263

GI number: 146299263

Start: 1730354

End: 1731598

Strand: Direct

Name: cysNC [H]

Synonym: Fjoh_1503

Alternate gene names: 146299263

Gene position: 1730354-1731598 (Clockwise)

Preceding gene: 146299262

Following gene: 146299264

Centisome position: 28.38

GC content: 34.38

Gene sequence:

>1245_bases
ATGGACGTTTTAAAAATAGCAACAGCAGGAAGTGTAGATGACGGAAAAAGTACTTTGATCGGGAGATTATTGTACGATAC
AAAATCATTGACAACTGATAAAATAGAAGCAATCGAAAAAAGCAGTAAGCAAAAAGGATACGATTACCTTGATTTTTCTT
TGGCAACTGATGGTTTAGTAGCAGAAAGAGAACAAGGAATCACGATTGACGTTGCGCATATTTATTTTTCGACTGCAAAG
AAAAGTTACATTATTGCCGATACTCCGGGTCACGTAGAATATACAAGAAACATGGTTACAGGAGCTTCGACTTCTCAGGT
TTCTATCATTTTAATTGATGCCAGAAAAGGAGTTATCGAGCAGACTTACCGTCACTTTTTTATCAATAATTTATTGAGAG
TAAAAGAGGTAATCGTAGCGATAAACAAAATGGATTTAGTTGATTACTCAGAAGAAGTTTTCAATAAAATCAAAGCCGAT
TTTCAGGCATTAAATGCAAAAAGCACATTCAAAGAGCAAAACGTAAGTTATATTCCGTTAAGCGCTATTAACGGCGGAAA
CGTGGTTGATAAATCTGAAAATATGCCTTGGTATGATGGACAAACGGTTTTAGAACATTTAGAAGGATTACATTCACATG
ATGTTTTTGAAGCAGGAAAAGCACGTTTTCCAGTTCAGACTGTTATTCGTCCAAAAACGGAAGAATACCATGATTTTAGA
GGTTACGCTGGTAAATTATACGGAAACTCAATTAAAGTTGGAGATGCTGTTACAGTTCTTCCATCTTTAACAGAATCTAA
AGTGTCTAAAATTCACTTTTTCGATAAAACATTTGACGAAGCTGTTGCAGGTTCTTCAATTACAATCGAATTAGAAAATG
ATATCAATGTGACGAGAGGTGATATGATTGTAAAATCAGATGAACTTCCAAAAATTGAAAAAGACATTACAACGACAGTT
TGCTGGATGGACAGCAAAAAACTGGTTCCAGGAACAAAATATTTAGTACAACACAATACAAACAGAGTTTTAGCAAAAGT
AGAAAGCATTAAAAATACAATTGCTACAGATTATTCTGGAACGACAGAAGCTTCACAATTGGCCATCAACGAAATTGGAG
AAGTAACGATCAAATTAAGCAAACCGTTATATTTTGATTCATACAACGAAAACAAATCAAACGGAGCTTTCATCTTAATT
GATACAGCAACAAACACAACAGCAGGAGTAGGATTTATTAGGTAA

Upstream 100 bases:

>100_bases
ACAACAATAACAAAAAAAGCAGACTAAATATTTTGATGGTTTTAGTTGATGTTTTATAAAAACCAACAACCATAAACCAA
CAACCAACAACATAAAAAGA

Downstream 100 bases:

>100_bases
ACCTGCTATAAGCTTTAAGCAATAAGCCTTAAGCTTAAAAGATTGAAGACACAAAGTTTAAAAAGCTTATAGCCTACAGC
TTAAAGCCTAAAGCAAGAAA

Product: sulfate adenylyltransferase, large subunit

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDVLKIATAGSVDDGKSTLIGRLLYDTKSLTTDKIEAIEKSSKQKGYDYLDFSLATDGLVAEREQGITIDVAHIYFSTAK
KSYIIADTPGHVEYTRNMVTGASTSQVSIILIDARKGVIEQTYRHFFINNLLRVKEVIVAINKMDLVDYSEEVFNKIKAD
FQALNAKSTFKEQNVSYIPLSAINGGNVVDKSENMPWYDGQTVLEHLEGLHSHDVFEAGKARFPVQTVIRPKTEEYHDFR
GYAGKLYGNSIKVGDAVTVLPSLTESKVSKIHFFDKTFDEAVAGSSITIELENDINVTRGDMIVKSDELPKIEKDITTTV
CWMDSKKLVPGTKYLVQHNTNRVLAKVESIKNTIATDYSGTTEASQLAINEIGEVTIKLSKPLYFDSYNENKSNGAFILI
DTATNTTAGVGFIR

Sequences:

>Translated_414_residues
MDVLKIATAGSVDDGKSTLIGRLLYDTKSLTTDKIEAIEKSSKQKGYDYLDFSLATDGLVAEREQGITIDVAHIYFSTAK
KSYIIADTPGHVEYTRNMVTGASTSQVSIILIDARKGVIEQTYRHFFINNLLRVKEVIVAINKMDLVDYSEEVFNKIKAD
FQALNAKSTFKEQNVSYIPLSAINGGNVVDKSENMPWYDGQTVLEHLEGLHSHDVFEAGKARFPVQTVIRPKTEEYHDFR
GYAGKLYGNSIKVGDAVTVLPSLTESKVSKIHFFDKTFDEAVAGSSITIELENDINVTRGDMIVKSDELPKIEKDITTTV
CWMDSKKLVPGTKYLVQHNTNRVLAKVESIKNTIATDYSGTTEASQLAINEIGEVTIKLSKPLYFDSYNENKSNGAFILI
DTATNTTAGVGFIR
>Mature_414_residues
MDVLKIATAGSVDDGKSTLIGRLLYDTKSLTTDKIEAIEKSSKQKGYDYLDFSLATDGLVAEREQGITIDVAHIYFSTAK
KSYIIADTPGHVEYTRNMVTGASTSQVSIILIDARKGVIEQTYRHFFINNLLRVKEVIVAINKMDLVDYSEEVFNKIKAD
FQALNAKSTFKEQNVSYIPLSAINGGNVVDKSENMPWYDGQTVLEHLEGLHSHDVFEAGKARFPVQTVIRPKTEEYHDFR
GYAGKLYGNSIKVGDAVTVLPSLTESKVSKIHFFDKTFDEAVAGSSITIELENDINVTRGDMIVKSDELPKIEKDITTTV
CWMDSKKLVPGTKYLVQHNTNRVLAKVESIKNTIATDYSGTTEASQLAINEIGEVTIKLSKPLYFDSYNENKSNGAFILI
DTATNTTAGVGFIR

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=430, Percent_Identity=26.046511627907, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI5729864, Length=430, Percent_Identity=26.046511627907, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4503475, Length=431, Percent_Identity=26.6821345707657, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI4503471, Length=430, Percent_Identity=26.046511627907, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI194018522, Length=294, Percent_Identity=30.952380952381, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI194097354, Length=294, Percent_Identity=30.952380952381, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI194018520, Length=294, Percent_Identity=30.952380952381, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI46094014, Length=293, Percent_Identity=30.3754266211604, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI34147630, Length=148, Percent_Identity=36.4864864864865, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=35.4838709677419, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789108, Length=414, Percent_Identity=46.8599033816425, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1789737, Length=143, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790412, Length=143, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17552884, Length=431, Percent_Identity=28.3062645011601, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=431, Percent_Identity=28.3062645011601, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI32566629, Length=423, Percent_Identity=26.7139479905437, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=424, Percent_Identity=26.6509433962264, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532067, Length=316, Percent_Identity=26.5822784810127, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=316, Percent_Identity=26.5822784810127, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32566301, Length=142, Percent_Identity=35.2112676056338, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=205, Percent_Identity=28.780487804878, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=150, Percent_Identity=34, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=437, Percent_Identity=25.858123569794, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=437, Percent_Identity=25.858123569794, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322937, Length=450, Percent_Identity=25.7777777777778, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=305, Percent_Identity=30.4918032786885, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6324761, Length=300, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652838, Length=438, Percent_Identity=27.1689497716895, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137572, Length=438, Percent_Identity=27.1689497716895, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI45553807, Length=431, Percent_Identity=28.538283062645, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45553816, Length=431, Percent_Identity=28.538283062645, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24651721, Length=431, Percent_Identity=28.538283062645, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17864154, Length=431, Percent_Identity=28.538283062645, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45550900, Length=430, Percent_Identity=26.2790697674419, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17137380, Length=296, Percent_Identity=26.6891891891892, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19921738, Length=179, Percent_Identity=32.4022346368715, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281363316, Length=291, Percent_Identity=25.4295532646048, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864358, Length=291, Percent_Identity=25.4295532646048, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 45918; Mature: 45918

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLKIATAGSVDDGKSTLIGRLLYDTKSLTTDKIEAIEKSSKQKGYDYLDFSLATDGLV
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCH
AEREQGITIDVAHIYFSTAKKSYIIADTPGHVEYTRNMVTGASTSQVSIILIDARKGVIE
HHCCCCCEEEEEEEEEECCCCCEEEECCCCCHHHHHHEECCCCCCEEEEEEEECCCCHHH
QTYRHFFINNLLRVKEVIVAINKMDLVDYSEEVFNKIKADFQALNAKSTFKEQNVSYIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEE
SAINGGNVVDKSENMPWYDGQTVLEHLEGLHSHDVFEAGKARFPVQTVIRPKTEEYHDFR
EECCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHCCCCCHHHHHHC
GYAGKLYGNSIKVGDAVTVLPSLTESKVSKIHFFDKTFDEAVAGSSITIELENDINVTRG
CCCEEEECCEEECCCEEEECCCCHHHHHHEEEEHHHHHHHHHCCCEEEEEECCCCCEECC
DMIVKSDELPKIEKDITTTVCWMDSKKLVPGTKYLVQHNTNRVLAKVESIKNTIATDYSG
CEEEECCCCCCHHHCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
TTEASQLAINEIGEVTIKLSKPLYFDSYNENKSNGAFILIDTATNTTAGVGFIR
CCHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDVLKIATAGSVDDGKSTLIGRLLYDTKSLTTDKIEAIEKSSKQKGYDYLDFSLATDGLV
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCH
AEREQGITIDVAHIYFSTAKKSYIIADTPGHVEYTRNMVTGASTSQVSIILIDARKGVIE
HHCCCCCEEEEEEEEEECCCCCEEEECCCCCHHHHHHEECCCCCCEEEEEEEECCCCHHH
QTYRHFFINNLLRVKEVIVAINKMDLVDYSEEVFNKIKADFQALNAKSTFKEQNVSYIPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEE
SAINGGNVVDKSENMPWYDGQTVLEHLEGLHSHDVFEAGKARFPVQTVIRPKTEEYHDFR
EECCCCCEEECCCCCCCCCCHHHHHHHHCCCCCCHHHCCCCCCCHHHHCCCCCHHHHHHC
GYAGKLYGNSIKVGDAVTVLPSLTESKVSKIHFFDKTFDEAVAGSSITIELENDINVTRG
CCCEEEECCEEECCCEEEECCCCHHHHHHEEEEHHHHHHHHHCCCEEEEEECCCCCEECC
DMIVKSDELPKIEKDITTTVCWMDSKKLVPGTKYLVQHNTNRVLAKVESIKNTIATDYSG
CEEEECCCCCCHHHCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
TTEASQLAINEIGEVTIKLSKPLYFDSYNENKSNGAFILIDTATNTTAGVGFIR
CCHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]