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The map label for this gene is metZ [H]

Identifier: 146299256

GI number: 146299256

Start: 1723900

End: 1725072

Strand: Direct

Name: metZ [H]

Synonym: Fjoh_1496

Alternate gene names: 146299256

Gene position: 1723900-1725072 (Clockwise)

Preceding gene: 146299255

Following gene: 146299257

Centisome position: 28.28

GC content: 36.57

Gene sequence:

>1173_bases
ATGAACACAGAAGAATTTGGTTTTGAAACACAAGCCATTAGAACACAATTAGAAAGATCACAATATTTAGAACATTCGGT
GCCTTTGTACCTGTCGTCAAGCTTTGTTTTTGAAGATGCCGAAGATATGAGAGCTTCTTTTACAGAAGAAAAAGAAAGAA
ATATTTACAGCCGTTTCAGCAATCCAAACACAACTGAGTTTGTGGAGAAAATCTGCAAAATGGAAGGAGCTGAATCTGGT
TATGCATTCGCAACAGGAATGGCTGCGGTATATTCTACTTTTGCCGGTTTATTGAATTCAGGCGATCATATCGTTTCTGC
AAGCAGTGTTTTTGGTTCTACTCACGCTTTGTTTATGACTTATTTTCCAAAATGGAATATCGAAACTTCTTATTTCGAAA
TTAATAAGCCGGAAACAATCGAAAGTTTCATCAAACCAAACACTAAAATTTTATACGCCGAATCTCCAACAAATCCCGGA
GTAGATGTTATCGATTTAGAATTGTTAGGAAATATTGCCAAAAAACACAACTTGATTTTAATTATCGACAACTGTTTTGC
AACGCCATATTTACAACAGCCAATTAAATTTGGGGCGCATTTAGTAATTCATTCTGCTACAAAATTAATCGACGGACAAG
GCCGCGTTTTAGGCGGGGTAACAGTTGGTAATGCCGATTTGATCAGACAGATTTATTTGTTTTCAAGAAATACAGGACCG
GCATTATCACCATTTAATGCTTGGGTTCTTTCAAAAAGTTTAGAAACTTTAGCCGTTCGTGTCGACAGACACTGCGAAAA
TGCTTTAAAAGTAGCTGAGTTTTTAGAAAGTCATCCAAATGTAAACAGCGTAAAATATCCGTTCTTGAAATCGCATCCAC
AGTATGAAATTGCTAAAAAGCAAATGAAAGCGGGTGGAAACATTATTGCATTTGAAATTAAAGGTGGTATCGAAGCAGGA
AGAAAATTTCTGAATTCAATCGAACTTTGCTCATTATCTGCAAATATTGGTGATACAAGAACAATCGTAACGCATCCGGC
ATCGACAACGCACAGTAAATTATCTGAAGAAGATCAATTGGCAGTAGGAATCACGCAAGGTCTTGTTCGTGTTTCTGTAG
GTTTAGAAACTGTAGAAGATGTAATTGCCGATTTAAAACAAGCGCTTGGATAA

Upstream 100 bases:

>100_bases
TGTTAATTGAAAAAAACTAGAAAGATTATTTTGCGAAACTCTGTTTTCACTTTGTGAATCTTCGCGAAATAAACCGTTCA
AAAAACAAAAAAACAACACA

Downstream 100 bases:

>100_bases
ATCTCTTAGATTTAAAAATTCAAATTATATATTAATGACGATTTTTGACTGTAAATTTATTACTGTTAAAAATCGTCATT
CTTTTTGAGTGATACTTGAG

Product: cystathionine gamma-lyase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNTEEFGFETQAIRTQLERSQYLEHSVPLYLSSSFVFEDAEDMRASFTEEKERNIYSRFSNPNTTEFVEKICKMEGAESG
YAFATGMAAVYSTFAGLLNSGDHIVSASSVFGSTHALFMTYFPKWNIETSYFEINKPETIESFIKPNTKILYAESPTNPG
VDVIDLELLGNIAKKHNLILIIDNCFATPYLQQPIKFGAHLVIHSATKLIDGQGRVLGGVTVGNADLIRQIYLFSRNTGP
ALSPFNAWVLSKSLETLAVRVDRHCENALKVAEFLESHPNVNSVKYPFLKSHPQYEIAKKQMKAGGNIIAFEIKGGIEAG
RKFLNSIELCSLSANIGDTRTIVTHPASTTHSKLSEEDQLAVGITQGLVRVSVGLETVEDVIADLKQALG

Sequences:

>Translated_390_residues
MNTEEFGFETQAIRTQLERSQYLEHSVPLYLSSSFVFEDAEDMRASFTEEKERNIYSRFSNPNTTEFVEKICKMEGAESG
YAFATGMAAVYSTFAGLLNSGDHIVSASSVFGSTHALFMTYFPKWNIETSYFEINKPETIESFIKPNTKILYAESPTNPG
VDVIDLELLGNIAKKHNLILIIDNCFATPYLQQPIKFGAHLVIHSATKLIDGQGRVLGGVTVGNADLIRQIYLFSRNTGP
ALSPFNAWVLSKSLETLAVRVDRHCENALKVAEFLESHPNVNSVKYPFLKSHPQYEIAKKQMKAGGNIIAFEIKGGIEAG
RKFLNSIELCSLSANIGDTRTIVTHPASTTHSKLSEEDQLAVGITQGLVRVSVGLETVEDVIADLKQALG
>Mature_390_residues
MNTEEFGFETQAIRTQLERSQYLEHSVPLYLSSSFVFEDAEDMRASFTEEKERNIYSRFSNPNTTEFVEKICKMEGAESG
YAFATGMAAVYSTFAGLLNSGDHIVSASSVFGSTHALFMTYFPKWNIETSYFEINKPETIESFIKPNTKILYAESPTNPG
VDVIDLELLGNIAKKHNLILIIDNCFATPYLQQPIKFGAHLVIHSATKLIDGQGRVLGGVTVGNADLIRQIYLFSRNTGP
ALSPFNAWVLSKSLETLAVRVDRHCENALKVAEFLESHPNVNSVKYPFLKSHPQYEIAKKQMKAGGNIIAFEIKGGIEAG
RKFLNSIELCSLSANIGDTRTIVTHPASTTHSKLSEEDQLAVGITQGLVRVSVGLETVEDVIADLKQALG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=386, Percent_Identity=34.9740932642487, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI299473759, Length=386, Percent_Identity=32.1243523316062, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI25453487, Length=385, Percent_Identity=29.8701298701299, Blast_Score=165, Evalue=7e-41,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=28.7598944591029, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1789383, Length=359, Percent_Identity=26.7409470752089, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=388, Percent_Identity=34.5360824742268, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=388, Percent_Identity=34.5360824742268, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=36.6576819407008, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6319307, Length=342, Percent_Identity=38.5964912280702, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=34.8780487804878, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6321254, Length=412, Percent_Identity=25, Blast_Score=126, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=319, Percent_Identity=24.4514106583072, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=374, Percent_Identity=27.5401069518717, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922554, Length=388, Percent_Identity=34.7938144329897, Blast_Score=244, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43073; Mature: 43073

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEEFGFETQAIRTQLERSQYLEHSVPLYLSSSFVFEDAEDMRASFTEEKERNIYSRFS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCHHHHHHHHHHHHHHHHHHHCC
NPNTTEFVEKICKMEGAESGYAFATGMAAVYSTFAGLLNSGDHIVSASSVFGSTHALFMT
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHEEEEE
YFPKWNIETSYFEINKPETIESFIKPNTKILYAESPTNPGVDVIDLELLGNIAKKHNLIL
ECCCCCCCCCEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCEEE
IIDNCFATPYLQQPIKFGAHLVIHSATKLIDGQGRVLGGVTVGNADLIRQIYLFSRNTGP
EEECCCCCHHHHHHHHHCCEEEEEHHHHHCCCCCCEEEEEEECCHHHHHHHHHHCCCCCC
ALSPFNAWVLSKSLETLAVRVDRHCENALKVAEFLESHPNVNSVKYPFLKSHPQYEIAKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHCCCCCHHHHHH
QMKAGGNIIAFEIKGGIEAGRKFLNSIELCSLSANIGDTRTIVTHPASTTHSKLSEEDQL
HHHCCCCEEEEEECCCHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHHHHCCCCCCE
AVGITQGLVRVSVGLETVEDVIADLKQALG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTEEFGFETQAIRTQLERSQYLEHSVPLYLSSSFVFEDAEDMRASFTEEKERNIYSRFS
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCEEECCHHHHHHHHHHHHHHHHHHHCC
NPNTTEFVEKICKMEGAESGYAFATGMAAVYSTFAGLLNSGDHIVSASSVFGSTHALFMT
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHEEEEE
YFPKWNIETSYFEINKPETIESFIKPNTKILYAESPTNPGVDVIDLELLGNIAKKHNLIL
ECCCCCCCCCEEECCCCHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCEEE
IIDNCFATPYLQQPIKFGAHLVIHSATKLIDGQGRVLGGVTVGNADLIRQIYLFSRNTGP
EEECCCCCHHHHHHHHHCCEEEEEHHHHHCCCCCCEEEEEEECCHHHHHHHHHHCCCCCC
ALSPFNAWVLSKSLETLAVRVDRHCENALKVAEFLESHPNVNSVKYPFLKSHPQYEIAKK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHCCCCCHHHHHH
QMKAGGNIIAFEIKGGIEAGRKFLNSIELCSLSANIGDTRTIVTHPASTTHSKLSEEDQL
HHHCCCCEEEEEECCCHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCHHHHCCCCCCE
AVGITQGLVRVSVGLETVEDVIADLKQALG
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]