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The map label for this gene is mdeA [H]

Identifier: 146299251

GI number: 146299251

Start: 1716225

End: 1717529

Strand: Direct

Name: mdeA [H]

Synonym: Fjoh_1491

Alternate gene names: 146299251

Gene position: 1716225-1717529 (Clockwise)

Preceding gene: 146299250

Following gene: 146299252

Centisome position: 28.15

GC content: 37.93

Gene sequence:

>1305_bases
ATGAGCACACAAAAATTTGCAACAAACGCATTACACGCAGGACACGATGTTACCAAAAATTCAGGAACAAGAGCAGTACC
AATTTATCAGACATCATCATACGTATTTAATAATGCCGATCATGCTGCCAATTTATTCGGTCTTGCCGAAGCCGGATTTA
TTTACACAAGATTAAATAACCCAACAAACGATGTTTTAGAACAGAGGCTTGCCACACTCGAAGGCGGTATTGGAGCTGTA
GTTACGGCATCTGGAGCATCAGCAATTTCTACCACTTTATTAACTTTATTAAGAACCGGCGATCATATCGTCGCATCAAA
TAGTTTATACGGCGGTACTTACAACTTATTAAGCGTAACATTACCTCGTTTAGGAATTACAACCACTTTTGTAGACCCTT
CAGATCCACAAAATTTCACTAAAGCAGCAAAAGAAAATACCAGAGCATTTTTTGTAGAATCTTTAGGAAATCCAAAATTA
GACGTATTAGACTTAAAAGCAATTTCGGCAGAAGCCAAAGCATTTAAAGTGCCATTTATAGTAGACAATACAGTTGCGAC
ACCTTATTTATTAAACCCAATTAAATATGGTGCCGATATTGTAATTCACTCATTAACTAAATATATAGCCGGAAACGGAA
CATCATTAGGCGGTGTAATTATCGATGCCGGAAACTTTGACTGGGCTAATGGAAAATTTCCTGAATTTACAGAGCCGTCT
GCAGGATATCACGGATTAGTATATCACGAAGCTTTAGGAAATGCAGCCTTTATTGCAAAAGCAAGAATCGAAGGATTACG
AGATTTCGGAGCTGCTTTGAGTCCGTTTAATGCTTTTCAGATAATTCAGGGATTAGAAACACTTCCAATCCGAATTAAAA
AACACAGTGAAAATGCTTTGGTTCTGGCCGAATGGTTAGAAAAACAAGATGAGGTAGTCTGGGTAAATTATCCAGGTTTG
AAATCAAATAAATATTATGATTTAGCCAAAGAATATCTTCCGGAAGGGCAAAGCGGTATTATCACCTTTGGATTAAAAGG
AGGTTTTGACGCCGCTAAAAAAGTAGTAGACGAAACGAAGTTATTCTCGCTTTTGGCCAATATTGGCGACACAAAATCGC
TGATTATTCACCCTGCAAGCACTACTCACCAGCAATTGTCTGAAGAAGACCAATTGGCTACAGGAGTATCAAAAGATTTA
GTCCGACTTTCTGTTGGAATAGAAGATGTTGAAGATTTAATTGCCGACTTACAAGCGGTTTTTGAAAGCGTAACCCAATC
GCAGTACAGCATCAATAAGAATTAG

Upstream 100 bases:

>100_bases
ATAGAAGGAGAATTTTCTTTTGCTTAATCACCACAAAATTTAATTACAATCAGATAACAATATATAATAGTATTAACAAT
ACCTAAAAAACTAAGAAATC

Downstream 100 bases:

>100_bases
GTTTTTTTGTTTTTTGTTTGAAAAATTGCCTTTAGCAGCGTGAGTTCTGCTAGAGGTGATTTTTTATGAAAAGAAAATCA
TCATACAGATATATTTTAAA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSTQKFATNALHAGHDVTKNSGTRAVPIYQTSSYVFNNADHAANLFGLAEAGFIYTRLNNPTNDVLEQRLATLEGGIGAV
VTASGASAISTTLLTLLRTGDHIVASNSLYGGTYNLLSVTLPRLGITTTFVDPSDPQNFTKAAKENTRAFFVESLGNPKL
DVLDLKAISAEAKAFKVPFIVDNTVATPYLLNPIKYGADIVIHSLTKYIAGNGTSLGGVIIDAGNFDWANGKFPEFTEPS
AGYHGLVYHEALGNAAFIAKARIEGLRDFGAALSPFNAFQIIQGLETLPIRIKKHSENALVLAEWLEKQDEVVWVNYPGL
KSNKYYDLAKEYLPEGQSGIITFGLKGGFDAAKKVVDETKLFSLLANIGDTKSLIIHPASTTHQQLSEEDQLATGVSKDL
VRLSVGIEDVEDLIADLQAVFESVTQSQYSINKN

Sequences:

>Translated_434_residues
MSTQKFATNALHAGHDVTKNSGTRAVPIYQTSSYVFNNADHAANLFGLAEAGFIYTRLNNPTNDVLEQRLATLEGGIGAV
VTASGASAISTTLLTLLRTGDHIVASNSLYGGTYNLLSVTLPRLGITTTFVDPSDPQNFTKAAKENTRAFFVESLGNPKL
DVLDLKAISAEAKAFKVPFIVDNTVATPYLLNPIKYGADIVIHSLTKYIAGNGTSLGGVIIDAGNFDWANGKFPEFTEPS
AGYHGLVYHEALGNAAFIAKARIEGLRDFGAALSPFNAFQIIQGLETLPIRIKKHSENALVLAEWLEKQDEVVWVNYPGL
KSNKYYDLAKEYLPEGQSGIITFGLKGGFDAAKKVVDETKLFSLLANIGDTKSLIIHPASTTHQQLSEEDQLATGVSKDL
VRLSVGIEDVEDLIADLQAVFESVTQSQYSINKN
>Mature_433_residues
STQKFATNALHAGHDVTKNSGTRAVPIYQTSSYVFNNADHAANLFGLAEAGFIYTRLNNPTNDVLEQRLATLEGGIGAVV
TASGASAISTTLLTLLRTGDHIVASNSLYGGTYNLLSVTLPRLGITTTFVDPSDPQNFTKAAKENTRAFFVESLGNPKLD
VLDLKAISAEAKAFKVPFIVDNTVATPYLLNPIKYGADIVIHSLTKYIAGNGTSLGGVIIDAGNFDWANGKFPEFTEPSA
GYHGLVYHEALGNAAFIAKARIEGLRDFGAALSPFNAFQIIQGLETLPIRIKKHSENALVLAEWLEKQDEVVWVNYPGLK
SNKYYDLAKEYLPEGQSGIITFGLKGGFDAAKKVVDETKLFSLLANIGDTKSLIIHPASTTHQQLSEEDQLATGVSKDLV
RLSVGIEDVEDLIADLQAVFESVTQSQYSINKN

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=30.8641975308642, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI25453487, Length=405, Percent_Identity=28.8888888888889, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI299473759, Length=406, Percent_Identity=27.5862068965517, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1790375, Length=426, Percent_Identity=32.1596244131455, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789383, Length=433, Percent_Identity=29.3302540415704, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=408, Percent_Identity=35.0490196078431, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=33.2530120481928, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17560056, Length=407, Percent_Identity=33.1695331695332, Blast_Score=204, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=47.7168949771689, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319307, Length=431, Percent_Identity=34.5707656612529, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=466, Percent_Identity=27.2532188841202, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=354, Percent_Identity=30.225988700565, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI19922554, Length=420, Percent_Identity=31.6666666666667, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46805; Mature: 46674

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQKFATNALHAGHDVTKNSGTRAVPIYQTSSYVFNNADHAANLFGLAEAGFIYTRLNN
CCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEECCCCHHHHHHHHHCCCEEEEECCC
PTNDVLEQRLATLEGGIGAVVTASGASAISTTLLTLLRTGDHIVASNSLYGGTYNLLSVT
CHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHEE
LPRLGITTTFVDPSDPQNFTKAAKENTRAFFVESLGNPKLDVLDLKAISAEAKAFKVPFI
CCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHCCCCCEEECCEE
VDNTVATPYLLNPIKYGADIVIHSLTKYIAGNGTSLGGVIIDAGNFDWANGKFPEFTEPS
ECCCCCCCHHCCHHHHCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
AGYHGLVYHEALGNAAFIAKARIEGLRDFGAALSPFNAFQIIQGLETLPIRIKKHSENAL
CCCCEEEEHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEE
VLAEWLEKQDEVVWVNYPGLKSNKYYDLAKEYLPEGQSGIITFGLKGGFDAAKKVVDETK
EHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
LFSLLANIGDTKSLIIHPASTTHQQLSEEDQLATGVSKDLVRLSVGIEDVEDLIADLQAV
HHHHHHCCCCCCEEEEECCCCHHHHHCHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHH
FESVTQSQYSINKN
HHHHHHHHHCCCCC
>Mature Secondary Structure 
STQKFATNALHAGHDVTKNSGTRAVPIYQTSSYVFNNADHAANLFGLAEAGFIYTRLNN
CCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEECCCCHHHHHHHHHCCCEEEEECCC
PTNDVLEQRLATLEGGIGAVVTASGASAISTTLLTLLRTGDHIVASNSLYGGTYNLLSVT
CHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHEE
LPRLGITTTFVDPSDPQNFTKAAKENTRAFFVESLGNPKLDVLDLKAISAEAKAFKVPFI
CCCCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHCCCCCEEECCEE
VDNTVATPYLLNPIKYGADIVIHSLTKYIAGNGTSLGGVIIDAGNFDWANGKFPEFTEPS
ECCCCCCCHHCCHHHHCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
AGYHGLVYHEALGNAAFIAKARIEGLRDFGAALSPFNAFQIIQGLETLPIRIKKHSENAL
CCCCEEEEHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCEE
VLAEWLEKQDEVVWVNYPGLKSNKYYDLAKEYLPEGQSGIITFGLKGGFDAAKKVVDETK
EHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
LFSLLANIGDTKSLIIHPASTTHQQLSEEDQLATGVSKDLVRLSVGIEDVEDLIADLQAV
HHHHHHCCCCCCEEEEECCCCHHHHHCHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHH
FESVTQSQYSINKN
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]