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The map label for this gene is emrB [H]

Identifier: 146299115

GI number: 146299115

Start: 1551134

End: 1552717

Strand: Direct

Name: emrB [H]

Synonym: Fjoh_1355

Alternate gene names: 146299115

Gene position: 1551134-1552717 (Clockwise)

Preceding gene: 146299114

Following gene: 146299116

Centisome position: 25.44

GC content: 37.75

Gene sequence:

>1584_bases
ATGGAAGATAAAAGTATTTTTAAATCATGGGTTCCAAAATGGGCAATCATTACAATTTTGTTTGTTTGCCTTTTGCATTC
CATGATTTTATTGGGAGTTTATACGTCAAATGTAACGTATGCGGCAAGTTTTCTGGACATTGAGCCCGAAGATTTGCAGT
ACGCGATGTGCGTAACGTATGGAACTCTGCTGGCTACAATTCTCATAGAAAGCCGATTTTCGAGTTTTTTTCCTGCCAAA
AATTACCTCATGGGTGTTTATTCCTTAATTGGAGTTACGATTGTAACGTCGGGTTATGTCGATAATTTTGCGGTTTTTCT
ATTGCTGAGAGTTGCCGAAGGAATCTTAATGGCGCTTCCTGCCATTACTATCAGACAATTGCTTATTGAGCAGTTTGATT
CTAAAAATGCCATTATAATTGGTTTTTCCTTTTATTATGGTTCTCTACTGCTGTCTACGCCTTTTATTATGAATATTGCA
GTTTGGTTTCTAGATCATTACGACTGGAAATACATGCTGTATGTTTCGGGCGGACTGCAGGTTTTAAATGTCTTTTTAAT
CTTGGTTACTTTTCGTGGGCACCGAACAACAAAGAAAATCCCGTTGTATCAAATCGACTGGATGAGCTACTTTTTGGTTT
TAACAGCGATTCTTTGCGGTGCCTACTTTTTTGTGTATGCCGAGAAAAAATATTGGTTCGAATCTTCTCAAATGGTTTTA
ATGCTTATTACGGCACTTATTACGGGAGGTTTGTTCATCTTCAAAGAGCTTTTGGTTAAAAGACCCACTTTTAATTTTGA
AGTTTTTAAATATGCCAATCTTCGAATTGGGTTTTTATTGTTTTTCCTGTTTTACATCAGCAGAGCAACGCTGAGTCTTT
GCCATTCGGCGATGTATTCGATTTGGAATTGGGATCCGTCTCGAGTTGCGGGTGTGCAATACATTAACGGACTCGGAAAT
ATTATCGGATTGGTTTTGGCGGCTTTTTTCTTGATGAAATCGCTTTCAACCAAAATCATTTTTCTGATTGGTTTTTCGCT
GATTGCCTTGTATCATTTTTGGTTTACGTTTCTTTTTGTGCCCGATGTGGCTTTGTCAGATATTATTGTTCCGTACTTTT
TGCAGGGCATCGGAGTTGGATTGTTATTTGTTCCGTTGATTTTATTTACTACTTCTTCGGTTCCTGCAAATATGGCGGTT
TCTTCGGGAATTGTCGGTGTTTCTGGACGTTTCTGGGGAAGTACGATTGGTTTTTGCGTTATGCAGAATGCAACGGTATT
TTTAAATAAAAAACACTTTTTAAAACTAAGTCAGTTTGTAACGGGCGAAAATCCCGAAGCACAGCAAAACATTGCAAACG
CTACGCAGAGTTTTATTGCCAAAGGATATTCCGCAGATAATGCGAATGTTTTAGCTATGAAAAAGATTTTTGGAAGTATT
ACCAAACAATCGACTTTATTGGCCGATATGGAGATTTACACCATTGTGGGTTACGGTTTATTGGTTTTGATAATTCTTAT
TGCCTGCAATCAGCATTTGAGACAGACTATGACTTTGGTGAAAAGCAAGATTTGGATTGGGTAG

Upstream 100 bases:

>100_bases
GAACAAAGAATTCCCGTTAGAATTCAGTTGAAAGACAGCGACAAACAAGCCGACTTTTTAAGAGCGGGAATGAATATTAC
TGTGATCGCAGCACACTAAA

Downstream 100 bases:

>100_bases
AGATTTTGTTTCAGGTTTAATGTTTCAGGTTCTAAGTTCTGCTCTCATTTTATGTCATTTCGACGAAGGAGAAATCTCCG
TAAGTAACTCCGCAACGAAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MEDKSIFKSWVPKWAIITILFVCLLHSMILLGVYTSNVTYAASFLDIEPEDLQYAMCVTYGTLLATILIESRFSSFFPAK
NYLMGVYSLIGVTIVTSGYVDNFAVFLLLRVAEGILMALPAITIRQLLIEQFDSKNAIIIGFSFYYGSLLLSTPFIMNIA
VWFLDHYDWKYMLYVSGGLQVLNVFLILVTFRGHRTTKKIPLYQIDWMSYFLVLTAILCGAYFFVYAEKKYWFESSQMVL
MLITALITGGLFIFKELLVKRPTFNFEVFKYANLRIGFLLFFLFYISRATLSLCHSAMYSIWNWDPSRVAGVQYINGLGN
IIGLVLAAFFLMKSLSTKIIFLIGFSLIALYHFWFTFLFVPDVALSDIIVPYFLQGIGVGLLFVPLILFTTSSVPANMAV
SSGIVGVSGRFWGSTIGFCVMQNATVFLNKKHFLKLSQFVTGENPEAQQNIANATQSFIAKGYSADNANVLAMKKIFGSI
TKQSTLLADMEIYTIVGYGLLVLIILIACNQHLRQTMTLVKSKIWIG

Sequences:

>Translated_527_residues
MEDKSIFKSWVPKWAIITILFVCLLHSMILLGVYTSNVTYAASFLDIEPEDLQYAMCVTYGTLLATILIESRFSSFFPAK
NYLMGVYSLIGVTIVTSGYVDNFAVFLLLRVAEGILMALPAITIRQLLIEQFDSKNAIIIGFSFYYGSLLLSTPFIMNIA
VWFLDHYDWKYMLYVSGGLQVLNVFLILVTFRGHRTTKKIPLYQIDWMSYFLVLTAILCGAYFFVYAEKKYWFESSQMVL
MLITALITGGLFIFKELLVKRPTFNFEVFKYANLRIGFLLFFLFYISRATLSLCHSAMYSIWNWDPSRVAGVQYINGLGN
IIGLVLAAFFLMKSLSTKIIFLIGFSLIALYHFWFTFLFVPDVALSDIIVPYFLQGIGVGLLFVPLILFTTSSVPANMAV
SSGIVGVSGRFWGSTIGFCVMQNATVFLNKKHFLKLSQFVTGENPEAQQNIANATQSFIAKGYSADNANVLAMKKIFGSI
TKQSTLLADMEIYTIVGYGLLVLIILIACNQHLRQTMTLVKSKIWIG
>Mature_527_residues
MEDKSIFKSWVPKWAIITILFVCLLHSMILLGVYTSNVTYAASFLDIEPEDLQYAMCVTYGTLLATILIESRFSSFFPAK
NYLMGVYSLIGVTIVTSGYVDNFAVFLLLRVAEGILMALPAITIRQLLIEQFDSKNAIIIGFSFYYGSLLLSTPFIMNIA
VWFLDHYDWKYMLYVSGGLQVLNVFLILVTFRGHRTTKKIPLYQIDWMSYFLVLTAILCGAYFFVYAEKKYWFESSQMVL
MLITALITGGLFIFKELLVKRPTFNFEVFKYANLRIGFLLFFLFYISRATLSLCHSAMYSIWNWDPSRVAGVQYINGLGN
IIGLVLAAFFLMKSLSTKIIFLIGFSLIALYHFWFTFLFVPDVALSDIIVPYFLQGIGVGLLFVPLILFTTSSVPANMAV
SSGIVGVSGRFWGSTIGFCVMQNATVFLNKKHFLKLSQFVTGENPEAQQNIANATQSFIAKGYSADNANVLAMKKIFGSI
TKQSTLLADMEIYTIVGYGLLVLIILIACNQHLRQTMTLVKSKIWIG

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=415, Percent_Identity=20.4819277108434, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 59478; Mature: 59478

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDKSIFKSWVPKWAIITILFVCLLHSMILLGVYTSNVTYAASFLDIEPEDLQYAMCVTY
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHEECCCHHHHHHHHHHHH
GTLLATILIESRFSSFFPAKNYLMGVYSLIGVTIVTSGYVDNFAVFLLLRVAEGILMALP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AITIRQLLIEQFDSKNAIIIGFSFYYGSLLLSTPFIMNIAVWFLDHYDWKYMLYVSGGLQ
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEECCCHH
VLNVFLILVTFRGHRTTKKIPLYQIDWMSYFLVLTAILCGAYFFVYAEKKYWFESSQMVL
HHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEECHHHHCCCCHHHH
MLITALITGGLFIFKELLVKRPTFNFEVFKYANLRIGFLLFFLFYISRATLSLCHSAMYS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWNWDPSRVAGVQYINGLGNIIGLVLAAFFLMKSLSTKIIFLIGFSLIALYHFWFTFLFV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PDVALSDIIVPYFLQGIGVGLLFVPLILFTTSSVPANMAVSSGIVGVSGRFWGSTIGFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEECCCCHHHHHHHHHH
MQNATVFLNKKHFLKLSQFVTGENPEAQQNIANATQSFIAKGYSADNANVLAMKKIFGSI
HCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TKQSTLLADMEIYTIVGYGLLVLIILIACNQHLRQTMTLVKSKIWIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDKSIFKSWVPKWAIITILFVCLLHSMILLGVYTSNVTYAASFLDIEPEDLQYAMCVTY
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHEECCCHHHHHHHHHHHH
GTLLATILIESRFSSFFPAKNYLMGVYSLIGVTIVTSGYVDNFAVFLLLRVAEGILMALP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AITIRQLLIEQFDSKNAIIIGFSFYYGSLLLSTPFIMNIAVWFLDHYDWKYMLYVSGGLQ
HHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEECCCHH
VLNVFLILVTFRGHRTTKKIPLYQIDWMSYFLVLTAILCGAYFFVYAEKKYWFESSQMVL
HHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEECHHHHCCCCHHHH
MLITALITGGLFIFKELLVKRPTFNFEVFKYANLRIGFLLFFLFYISRATLSLCHSAMYS
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IWNWDPSRVAGVQYINGLGNIIGLVLAAFFLMKSLSTKIIFLIGFSLIALYHFWFTFLFV
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PDVALSDIIVPYFLQGIGVGLLFVPLILFTTSSVPANMAVSSGIVGVSGRFWGSTIGFCV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEECCCCHHHHHHHHHH
MQNATVFLNKKHFLKLSQFVTGENPEAQQNIANATQSFIAKGYSADNANVLAMKKIFGSI
HCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TKQSTLLADMEIYTIVGYGLLVLIILIACNQHLRQTMTLVKSKIWIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]