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The map label for this gene is mexA [H]

Identifier: 146299027

GI number: 146299027

Start: 1443960

End: 1445027

Strand: Direct

Name: mexA [H]

Synonym: Fjoh_1267

Alternate gene names: 146299027

Gene position: 1443960-1445027 (Clockwise)

Preceding gene: 146299026

Following gene: 146299028

Centisome position: 23.68

GC content: 32.87

Gene sequence:

>1068_bases
ATGAAGAAAATAATTATAACAATCGTGATCATAGCCGTAGCTTTTTTCGGGATAAGCTATATCTTAAATAAAAATAAAGC
AGAAAACGAAGGTAAAACTGCTATCGTAGCCGAAAAAAATGCTGCAGTATCTGTAAAAGTAGCCACTGTTAAAACAGAAG
ATGTTAATCTTGGTTTTACTGCAAATGGAAACTTTGTGCCAATTCAGGAATTAACTTTCTCTGCTGAAAAATCTGGAAAA
GTAATCAGTGTTTTAGCCAAAGAAGGTGATTATGTAAGAGTAGGACAGACATTACTAACAGTAAGAGGTGATGTTATTAA
TGTAAATGCTCAGCAGGCTCAGGCTGTTTATCAAAATGCAAAATCTGATTACGAAAGATATGAAAACGCTTTTAAAACAG
GCGGTGTAACAAAACAACAATTAGATCAGGCAAAATTAGCTTTGACAAATGCACAATCTAACTTAACACAAGCTAATATC
AACGTTGGAGATACTAAAGTAAAAGCTCCAATTAACGGATACATCAATAAAAAATATATTGAACCGGGATCTATTTTAGC
AGGAATGCCGGCAACAGCTTTGTTTGATATCGTAAACGTTTCTAAATTAAAATTAACTGTTACAGTAAACGAAAGCCAGG
TTGCAAGCTTAAAATTAGGAAACTCAGTGAGCATTACTGCTAGTGTTTATCCTGATAAAACATTCTCTGGAAAAATTACT
TTCATCGCTGCAAAAGCAGATGCTTCATTAAACTTCCCTGTTGAAATCGAAATTACAAACAATGCTAATAATGACTTAAA
AGCAGGTATGTACGGAACAGCAAACTTTGGTTCAAACAACCAAAAACAAAACTTAAAAGTGGTTCCTAGAAATGCATTTG
TTGGAAGTGTAAGCAGTAACGAAATTTTCGTAGTTGAAAATAATGTAGCAAAATTGAAAAAAGTTGTTGCAGGAAGAATT
TTAGGAGATAAAGTTGAAATCATCAATGGTTTAAATGACGGAGACGTAGTAATTATTACAGGTCAGATCAACTTACAAGA
CGGAAATACAGTAGAAATTATTAAATAA

Upstream 100 bases:

>100_bases
AAATAATTATACAAGAGCAGTTTTAAATTATAAAATTGCCGAAATATCTCTAATCAAATCAAAAGGCGAACTTAAATCTC
TTACTAAATAACAAATTACA

Downstream 100 bases:

>100_bases
TTGTACCGCTTTAAGCCGTAAGCAATAAGCTTTAAGCCTTTTAAAAAAGCCTAAAGCCTACAGCGTATAGCCTACAGCCT
AAAACAAAATATATGAAATT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKKIIITIVIIAVAFFGISYILNKNKAENEGKTAIVAEKNAAVSVKVATVKTEDVNLGFTANGNFVPIQELTFSAEKSGK
VISVLAKEGDYVRVGQTLLTVRGDVINVNAQQAQAVYQNAKSDYERYENAFKTGGVTKQQLDQAKLALTNAQSNLTQANI
NVGDTKVKAPINGYINKKYIEPGSILAGMPATALFDIVNVSKLKLTVTVNESQVASLKLGNSVSITASVYPDKTFSGKIT
FIAAKADASLNFPVEIEITNNANNDLKAGMYGTANFGSNNQKQNLKVVPRNAFVGSVSSNEIFVVENNVAKLKKVVAGRI
LGDKVEIINGLNDGDVVIITGQINLQDGNTVEIIK

Sequences:

>Translated_355_residues
MKKIIITIVIIAVAFFGISYILNKNKAENEGKTAIVAEKNAAVSVKVATVKTEDVNLGFTANGNFVPIQELTFSAEKSGK
VISVLAKEGDYVRVGQTLLTVRGDVINVNAQQAQAVYQNAKSDYERYENAFKTGGVTKQQLDQAKLALTNAQSNLTQANI
NVGDTKVKAPINGYINKKYIEPGSILAGMPATALFDIVNVSKLKLTVTVNESQVASLKLGNSVSITASVYPDKTFSGKIT
FIAAKADASLNFPVEIEITNNANNDLKAGMYGTANFGSNNQKQNLKVVPRNAFVGSVSSNEIFVVENNVAKLKKVVAGRI
LGDKVEIINGLNDGDVVIITGQINLQDGNTVEIIK
>Mature_355_residues
MKKIIITIVIIAVAFFGISYILNKNKAENEGKTAIVAEKNAAVSVKVATVKTEDVNLGFTANGNFVPIQELTFSAEKSGK
VISVLAKEGDYVRVGQTLLTVRGDVINVNAQQAQAVYQNAKSDYERYENAFKTGGVTKQQLDQAKLALTNAQSNLTQANI
NVGDTKVKAPINGYINKKYIEPGSILAGMPATALFDIVNVSKLKLTVTVNESQVASLKLGNSVSITASVYPDKTFSGKIT
FIAAKADASLNFPVEIEITNNANNDLKAGMYGTANFGSNNQKQNLKVVPRNAFVGSVSSNEIFVVENNVAKLKKVVAGRI
LGDKVEIINGLNDGDVVIITGQINLQDGNTVEIIK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=344, Percent_Identity=25.2906976744186, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87082036, Length=341, Percent_Identity=24.633431085044, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081796, Length=372, Percent_Identity=21.7741935483871, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37960; Mature: 37960

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIIITIVIIAVAFFGISYILNKNKAENEGKTAIVAEKNAAVSVKVATVKTEDVNLGFT
CCEEEEHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCEEEEEEEEEEECCEEEEEE
ANGNFVPIQELTFSAEKSGKVISVLAKEGDYVRVGQTLLTVRGDVINVNAQQAQAVYQNA
CCCCEEEHHHEEECCCCCCCEEEEEECCCCEEEECCEEEEEECCEEECCHHHHHHHHHHH
KSDYERYENAFKTGGVTKQQLDQAKLALTNAQSNLTQANINVGDTKVKAPINGYINKKYI
HHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCCCEEEECCCCCEEEECCCCCCEEEEEE
EPGSILAGMPATALFDIVNVSKLKLTVTVNESQVASLKLGNSVSITASVYPDKTFSGKIT
CCCCEEECCCHHHHHHHCCEEEEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCCEEE
FIAAKADASLNFPVEIEITNNANNDLKAGMYGTANFGSNNQKQNLKVVPRNAFVGSVSSN
EEEEECCCCCCCCEEEEEECCCCCCEECCEEEECCCCCCCCCCCEEEEECCEEEECCCCC
EIFVVENNVAKLKKVVAGRILGDKVEIINGLNDGDVVIITGQINLQDGNTVEIIK
EEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEECCCCEEEEEC
>Mature Secondary Structure
MKKIIITIVIIAVAFFGISYILNKNKAENEGKTAIVAEKNAAVSVKVATVKTEDVNLGFT
CCEEEEHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCEEEEEEEEEEECCEEEEEE
ANGNFVPIQELTFSAEKSGKVISVLAKEGDYVRVGQTLLTVRGDVINVNAQQAQAVYQNA
CCCCEEEHHHEEECCCCCCCEEEEEECCCCEEEECCEEEEEECCEEECCHHHHHHHHHHH
KSDYERYENAFKTGGVTKQQLDQAKLALTNAQSNLTQANINVGDTKVKAPINGYINKKYI
HHHHHHHHHHHHCCCCCHHHHHHHHEEEECCCCCCEEEECCCCCEEEECCCCCCEEEEEE
EPGSILAGMPATALFDIVNVSKLKLTVTVNESQVASLKLGNSVSITASVYPDKTFSGKIT
CCCCEEECCCHHHHHHHCCEEEEEEEEEECCCEEEEEECCCEEEEEEEECCCCCCCCEEE
FIAAKADASLNFPVEIEITNNANNDLKAGMYGTANFGSNNQKQNLKVVPRNAFVGSVSSN
EEEEECCCCCCCCEEEEEECCCCCCEECCEEEECCCCCCCCCCCEEEEECCEEEECCCCC
EIFVVENNVAKLKKVVAGRILGDKVEIINGLNDGDVVIITGQINLQDGNTVEIIK
EEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]