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The map label for this gene is oprM [H]

Identifier: 146298670

GI number: 146298670

Start: 1025531

End: 1026898

Strand: Direct

Name: oprM [H]

Synonym: Fjoh_0908

Alternate gene names: 146298670

Gene position: 1025531-1026898 (Clockwise)

Preceding gene: 146298669

Following gene: 146298674

Centisome position: 16.82

GC content: 38.3

Gene sequence:

>1368_bases
ATGAAAACTCTAAAAATACTTCTAGCAATATTTTTGATAGCTGTTTTCCCTTACGGATGTATGGTTGGACCAAAGTATGC
CAAACCAGAACAGCCTCAAACAGATTCTTTCCGTTACAGCAATCAGTCTGCTGATAGCACAAAATCGGTTACGACTATAA
AATGGTCATCGATATTTAACGATGATGTCTTAATTGGTTTGATTGAAAAAGGAATCCAGAACAATTATGATCTTAAAATT
GCCATTGCTCGACTAGAGCAAGTACAAGCCAATCTTGGAATGGCAAAAGCCGATTTATTTCCTGCTTTTCAATATTCCGG
TCAGGTCAATAGTGCCGAAACTTTCATGCAGCCTTCATCAGTATTTGCCAATATGTCTTGGGAACTTGATTTTTGGGGAA
AATACCGTCATCAGACTAAAGCTTTACAAAATGAACTTTTAGCTTCTGATGAAGCCCGAAAAGTAGTATTGTCTAATATT
GTAAGTACAATTGCGACTTCTTATTTTGAACTGCGAGACTTTGATAATCAATTGGAAATTACCATTCACACTTTAGAAAC
CAGACAAAAAGCGTATGATATTATCAACGAACGTTTTATAAGCGGTTATGTTGCGGAATTGGATAAAGTACAAATCGAAC
AGCAGGTTGCCATTGCCGAAGCAAATATTCCGCTAATTAAAAGACAGATTACGGCTTTAGAAAATTCAATTTCAATTCTG
GTTGGACAAGTTCCACAGCCTATTCCAAGAGGAAAAACCAACAGTGAATTATTGATTTTAACAGAGTTCCCAACTTCTGT
ACCATCTGCATTACTGGAAAACAGGCCCGATGTAAAACGTCAGGAGTATTTGTATAAAGCCGCTTTTGAAAGAATTGGCG
TTGCACAAGCGTTGCGATATCCGTCATTTAACATTGGAGCGCTTGCCGGTTTTGCGAGTATTACGGTTGGAGATTTATTT
AACGACGGTTCTTACACACAAAATGTCGGCGCTGGAGTTGCAGGTCCGATCTTCAACTTCGGAAAAAACAAAAGACGTGT
TGAAGTCAATCGACAAATTGCAGAAGAAATGAAATTCAATTTCCAGAAAACCTATTTAACCGCAATTTCTGAAGTCGAGA
ACTCGCTCCAAAACGTGGCGATGTTTAAAGAAGAATGGACTGCCAGAAACAGGCAAGTCGTTGCGGCTCAAAAAAACTAC
GATCTTTCAAACGCCAGATATTACAATGGTTATGTTTCGTACTTAGAAGTTTTGGACGCACAAAGATCTTTGTTTGAAGC
GCAGTTGAGCCTTTCGCAATTAACTCAAAAACAATTAAGTTCGATGGTTCAGTTATATAAAGCGCTGGGAGGAGGATGGA
GCTTTTAA

Upstream 100 bases:

>100_bases
CTATTTTGGGAGTTTGTCTGATTCCCGTATTGTTTGTATTTATTGAAACCTTCGGAAAGAAAAAAACCGATGAAACCGAT
GAACCTAAAAAGGACGAACA

Downstream 100 bases:

>100_bases
AGGTTATGAGATGCTAAGACACTAAGTTTTTTGTTTTTATCTAACTATCATTCCGTAGGAATGTTTCATTGGTAGAAAAA
AACAGCAATCATCCGCATTT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKTLKILLAIFLIAVFPYGCMVGPKYAKPEQPQTDSFRYSNQSADSTKSVTTIKWSSIFNDDVLIGLIEKGIQNNYDLKI
AIARLEQVQANLGMAKADLFPAFQYSGQVNSAETFMQPSSVFANMSWELDFWGKYRHQTKALQNELLASDEARKVVLSNI
VSTIATSYFELRDFDNQLEITIHTLETRQKAYDIINERFISGYVAELDKVQIEQQVAIAEANIPLIKRQITALENSISIL
VGQVPQPIPRGKTNSELLILTEFPTSVPSALLENRPDVKRQEYLYKAAFERIGVAQALRYPSFNIGALAGFASITVGDLF
NDGSYTQNVGAGVAGPIFNFGKNKRRVEVNRQIAEEMKFNFQKTYLTAISEVENSLQNVAMFKEEWTARNRQVVAAQKNY
DLSNARYYNGYVSYLEVLDAQRSLFEAQLSLSQLTQKQLSSMVQLYKALGGGWSF

Sequences:

>Translated_455_residues
MKTLKILLAIFLIAVFPYGCMVGPKYAKPEQPQTDSFRYSNQSADSTKSVTTIKWSSIFNDDVLIGLIEKGIQNNYDLKI
AIARLEQVQANLGMAKADLFPAFQYSGQVNSAETFMQPSSVFANMSWELDFWGKYRHQTKALQNELLASDEARKVVLSNI
VSTIATSYFELRDFDNQLEITIHTLETRQKAYDIINERFISGYVAELDKVQIEQQVAIAEANIPLIKRQITALENSISIL
VGQVPQPIPRGKTNSELLILTEFPTSVPSALLENRPDVKRQEYLYKAAFERIGVAQALRYPSFNIGALAGFASITVGDLF
NDGSYTQNVGAGVAGPIFNFGKNKRRVEVNRQIAEEMKFNFQKTYLTAISEVENSLQNVAMFKEEWTARNRQVVAAQKNY
DLSNARYYNGYVSYLEVLDAQRSLFEAQLSLSQLTQKQLSSMVQLYKALGGGWSF
>Mature_455_residues
MKTLKILLAIFLIAVFPYGCMVGPKYAKPEQPQTDSFRYSNQSADSTKSVTTIKWSSIFNDDVLIGLIEKGIQNNYDLKI
AIARLEQVQANLGMAKADLFPAFQYSGQVNSAETFMQPSSVFANMSWELDFWGKYRHQTKALQNELLASDEARKVVLSNI
VSTIATSYFELRDFDNQLEITIHTLETRQKAYDIINERFISGYVAELDKVQIEQQVAIAEANIPLIKRQITALENSISIL
VGQVPQPIPRGKTNSELLILTEFPTSVPSALLENRPDVKRQEYLYKAAFERIGVAQALRYPSFNIGALAGFASITVGDLF
NDGSYTQNVGAGVAGPIFNFGKNKRRVEVNRQIAEEMKFNFQKTYLTAISEVENSLQNVAMFKEEWTARNRQVVAAQKNY
DLSNARYYNGYVSYLEVLDAQRSLFEAQLSLSQLTQKQLSSMVQLYKALGGGWSF

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=460, Percent_Identity=28.0434782608696, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI2367348, Length=477, Percent_Identity=23.0607966457023, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51240; Mature: 51240

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLKILLAIFLIAVFPYGCMVGPKYAKPEQPQTDSFRYSNQSADSTKSVTTIKWSSIFN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
DDVLIGLIEKGIQNNYDLKIAIARLEQVQANLGMAKADLFPAFQYSGQVNSAETFMQPSS
CCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHCHHHHCCCCCCCHHHHCCHHH
VFANMSWELDFWGKYRHQTKALQNELLASDEARKVVLSNIVSTIATSYFELRDFDNQLEI
HHCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TIHTLETRQKAYDIINERFISGYVAELDKVQIEQQVAIAEANIPLIKRQITALENSISIL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHE
VGQVPQPIPRGKTNSELLILTEFPTSVPSALLENRPDVKRQEYLYKAAFERIGVAQALRY
ECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PSFNIGALAGFASITVGDLFNDGSYTQNVGAGVAGPIFNFGKNKRRVEVNRQIAEEMKFN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEHHHHHHHHHHHHH
FQKTYLTAISEVENSLQNVAMFKEEWTARNRQVVAAQKNYDLSNARYYNGYVSYLEVLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHH
QRSLFEAQLSLSQLTQKQLSSMVQLYKALGGGWSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKTLKILLAIFLIAVFPYGCMVGPKYAKPEQPQTDSFRYSNQSADSTKSVTTIKWSSIFN
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
DDVLIGLIEKGIQNNYDLKIAIARLEQVQANLGMAKADLFPAFQYSGQVNSAETFMQPSS
CCHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHCHHHHCCCCCCCHHHHCCHHH
VFANMSWELDFWGKYRHQTKALQNELLASDEARKVVLSNIVSTIATSYFELRDFDNQLEI
HHCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TIHTLETRQKAYDIINERFISGYVAELDKVQIEQQVAIAEANIPLIKRQITALENSISIL
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHE
VGQVPQPIPRGKTNSELLILTEFPTSVPSALLENRPDVKRQEYLYKAAFERIGVAQALRY
ECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PSFNIGALAGFASITVGDLFNDGSYTQNVGAGVAGPIFNFGKNKRRVEVNRQIAEEMKFN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEHHHHHHHHHHHHH
FQKTYLTAISEVENSLQNVAMFKEEWTARNRQVVAAQKNYDLSNARYYNGYVSYLEVLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHH
QRSLFEAQLSLSQLTQKQLSSMVQLYKALGGGWSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]