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The map label for this gene is mexA [H]

Identifier: 146298668

GI number: 146298668

Start: 1021209

End: 1022384

Strand: Direct

Name: mexA [H]

Synonym: Fjoh_0906

Alternate gene names: 146298668

Gene position: 1021209-1022384 (Clockwise)

Preceding gene: 146298663

Following gene: 146298669

Centisome position: 16.75

GC content: 39.37

Gene sequence:

>1176_bases
ATGTGTATAATTTTAACATTTATGATTATGAATGCTTTAAAACTACCAATCCTCCCCCTCTTCTTTCTTTTAACCTTATT
TACTTCCTGCAAAAAAGAACAGCCCAAAGCTCCGCCTCCAATGCAGGCTCCTTTTGTTACGGTCAAAAGTGAAGATGTCC
CCATTTATAAAGATTTTGCAGGACAGACTTTTGGAGATTTAGACATTGAATTAATCGCAAGAGTTGATGGTATTCTCACC
GGAATTCATTTTAAAGAAGGCCAGAGAGTCAAAAAAGGCCAGCTTTTATATACCATCGACCCGTTAGAATATGATACCAA
AGTAGAGCAGGTTCGCGGACAAGTTGCCGGCGCTCAAAGTAATTTGGTAAATGCCGAAGAAGAATTAAAAAGAATCCGTC
CGCTTGCTGATATGAATGCAGTCAGTAAACGTGAATTAGATGCCGCCGTCGCAAAAGACAAAGCAGCACGATCTAACCTC
AACAGTGTACAGGCCAGTTTAAGAAATCAGCAGATTGAAAGAAGCTACTGCGACATCAGATCTCCAATTGATGGCGTAAT
TGGACTTTCCAATGCCCGATTAGGAGATTATATTACCCGAATTGGAAATGATGCGAAACTCAATACCGTTTCTAAACTAG
AAAAAGTAAGAGTACAATTTACGGTCAGTGAATCTGATTATTTGCGATATCAAAAGCAGGTCAAAGCCGGAGAACGTATT
ACTGATCTTTCTTTAATACTTTCTGACGGAAGTACCCACCCATACAAAGGAAGTCTCAATTTCTCCGACACTAAAATAGA
TCCTACAACGGGAACTGTAACCATCGAGGCGCAGTTTCCTAATCCAGATGGTACTTTACGATCCGGACAATTTGCAAAAG
TTCGTGTTTTAATTCGTACTCAAAAAGATGCCATTGTTGTCCCTCAAAAAGCAGTAACCGAAATTCAGGGACTTTTTCAG
GTTTCAATTATAGATTCTAAAAACACGATTCAAACCCGAATGGTTGAAGTCGGACAAAAAATTGGTGTCGACTGGATTAT
TACCAAAGGTTTAAAACCCGACGAAAAAGTCGCTATTATTGGAAATCAGTTTATTCAGCCCGGATCAACTGTTGTTCCCG
TTCCTTATGTAGCCGACAAAGATAAAAAGCAGATTGCATCATCTCTAAACAACTAG

Upstream 100 bases:

>100_bases
ATATTTTAATTTAAATTCTATCGTTTTAAATAAACAACATTGTTGCATTAATAAAAAACAATCTCTGCTTATCAATTGGT
TACAAAAATTTATTAAATTT

Downstream 100 bases:

>100_bases
ATTATGGATAATTTCTTTGTACGCAGACCAATCGTTGCTATTGTTCTCTCCATTTTCATTGTTTTAATTGGAGGCCTTTC
TGTCCTTTCAACACCTATTG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MCIILTFMIMNALKLPILPLFFLLTLFTSCKKEQPKAPPPMQAPFVTVKSEDVPIYKDFAGQTFGDLDIELIARVDGILT
GIHFKEGQRVKKGQLLYTIDPLEYDTKVEQVRGQVAGAQSNLVNAEEELKRIRPLADMNAVSKRELDAAVAKDKAARSNL
NSVQASLRNQQIERSYCDIRSPIDGVIGLSNARLGDYITRIGNDAKLNTVSKLEKVRVQFTVSESDYLRYQKQVKAGERI
TDLSLILSDGSTHPYKGSLNFSDTKIDPTTGTVTIEAQFPNPDGTLRSGQFAKVRVLIRTQKDAIVVPQKAVTEIQGLFQ
VSIIDSKNTIQTRMVEVGQKIGVDWIITKGLKPDEKVAIIGNQFIQPGSTVVPVPYVADKDKKQIASSLNN

Sequences:

>Translated_391_residues
MCIILTFMIMNALKLPILPLFFLLTLFTSCKKEQPKAPPPMQAPFVTVKSEDVPIYKDFAGQTFGDLDIELIARVDGILT
GIHFKEGQRVKKGQLLYTIDPLEYDTKVEQVRGQVAGAQSNLVNAEEELKRIRPLADMNAVSKRELDAAVAKDKAARSNL
NSVQASLRNQQIERSYCDIRSPIDGVIGLSNARLGDYITRIGNDAKLNTVSKLEKVRVQFTVSESDYLRYQKQVKAGERI
TDLSLILSDGSTHPYKGSLNFSDTKIDPTTGTVTIEAQFPNPDGTLRSGQFAKVRVLIRTQKDAIVVPQKAVTEIQGLFQ
VSIIDSKNTIQTRMVEVGQKIGVDWIITKGLKPDEKVAIIGNQFIQPGSTVVPVPYVADKDKKQIASSLNN
>Mature_391_residues
MCIILTFMIMNALKLPILPLFFLLTLFTSCKKEQPKAPPPMQAPFVTVKSEDVPIYKDFAGQTFGDLDIELIARVDGILT
GIHFKEGQRVKKGQLLYTIDPLEYDTKVEQVRGQVAGAQSNLVNAEEELKRIRPLADMNAVSKRELDAAVAKDKAARSNL
NSVQASLRNQQIERSYCDIRSPIDGVIGLSNARLGDYITRIGNDAKLNTVSKLEKVRVQFTVSESDYLRYQKQVKAGERI
TDLSLILSDGSTHPYKGSLNFSDTKIDPTTGTVTIEAQFPNPDGTLRSGQFAKVRVLIRTQKDAIVVPQKAVTEIQGLFQ
VSIIDSKNTIQTRMVEVGQKIGVDWIITKGLKPDEKVAIIGNQFIQPGSTVVPVPYVADKDKKQIASSLNN

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=365, Percent_Identity=31.7808219178082, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=31.3351498637602, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1786668, Length=379, Percent_Identity=33.245382585752, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87082036, Length=333, Percent_Identity=30.6306306306306, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081951, Length=129, Percent_Identity=32.5581395348837, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081796, Length=317, Percent_Identity=25.2365930599369, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43262; Mature: 43262

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCIILTFMIMNALKLPILPLFFLLTLFTSCKKEQPKAPPPMQAPFVTVKSEDVPIYKDFA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEHHHC
GQTFGDLDIELIARVDGILTGIHFKEGQRVKKGQLLYTIDPLEYDTKVEQVRGQVAGAQS
CCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHH
NLVNAEEELKRIRPLADMNAVSKRELDAAVAKDKAARSNLNSVQASLRNQQIERSYCDIR
HCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPIDGVIGLSNARLGDYITRIGNDAKLNTVSKLEKVRVQFTVSESDYLRYQKQVKAGERI
CCCHHHEECCCCHHHHHHHHCCCCCCHHHHHHHHEEEEEEEECCHHHHHHHHHHHCCCCE
TDLSLILSDGSTHPYKGSLNFSDTKIDPTTGTVTIEAQFPNPDGTLRSGQFAKVRVLIRT
EEEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEE
QKDAIVVPQKAVTEIQGLFQVSIIDSKNTIQTRMVEVGQKIGVDWIITKGLKPDEKVAII
CCCEEECCHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEE
GNQFIQPGSTVVPVPYVADKDKKQIASSLNN
CCCCCCCCCEEEECCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MCIILTFMIMNALKLPILPLFFLLTLFTSCKKEQPKAPPPMQAPFVTVKSEDVPIYKDFA
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEHHHC
GQTFGDLDIELIARVDGILTGIHFKEGQRVKKGQLLYTIDPLEYDTKVEQVRGQVAGAQS
CCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCHH
NLVNAEEELKRIRPLADMNAVSKRELDAAVAKDKAARSNLNSVQASLRNQQIERSYCDIR
HCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPIDGVIGLSNARLGDYITRIGNDAKLNTVSKLEKVRVQFTVSESDYLRYQKQVKAGERI
CCCHHHEECCCCHHHHHHHHCCCCCCHHHHHHHHEEEEEEEECCHHHHHHHHHHHCCCCE
TDLSLILSDGSTHPYKGSLNFSDTKIDPTTGTVTIEAQFPNPDGTLRSGQFAKVRVLIRT
EEEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEEE
QKDAIVVPQKAVTEIQGLFQVSIIDSKNTIQTRMVEVGQKIGVDWIITKGLKPDEKVAII
CCCEEECCHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEE
GNQFIQPGSTVVPVPYVADKDKKQIASSLNN
CCCCCCCCCEEEECCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]