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The map label for this gene is metR [H]

Identifier: 146298635

GI number: 146298635

Start: 986313

End: 987212

Strand: Direct

Name: metR [H]

Synonym: Fjoh_0873

Alternate gene names: 146298635

Gene position: 986313-987212 (Clockwise)

Preceding gene: 146298633

Following gene: 146298636

Centisome position: 16.18

GC content: 34.78

Gene sequence:

>900_bases
ATGGAAATACGTCATTTAAAATTGATAAAAGCAATTGTTGAAGAAGGAAGCATAACAAAAGCTATTGACAAACTTCACTT
GACACAATCGGCTTTGAGTCATCAGCTCAAAGAAGCAGAATATCAATTGGGTACGCCCATTTTTTTAAGAACCAATAAAA
AACTGGTTCTTACCAAAGCCGGAGAAAAAATCTATGAACTGGCAAATGAAATCCTAAACAAACTCACCGAAACCGAAAGT
CAGATCAAACAAATGGTTTTTGGAGAATATGGCGAAATTAGAATCAGTACAGAATGTTTTTCCAGTTATCACTGGCTTCC
ATCGGTTTTAAAGCAGTTTCACCTTTTATATCCAAATGTTGAATTGAAGATTGTTACCGAAGCCACTCATATTCCGCTGC
AAAAACTTTTAGAAAACACGATTGACATTGCCATTGTAAGTGACACTATAAAAGACCATCATATAAAATACACCGAATTA
TTTCAGGATGAAGTAGTAGTGGTGATTTCAGAAAACCATCCGTGGGCCGGCAAAAAGTATGTCGTTGCCGAAGATTTTGT
AAATGAACATTTAATTATTCATTCACTTCCTATGGAAACGGTTACAATTCATCAGTATTTTTTGGCTCCAGCCAAAGTAA
GTCCGAAAAAGATAACACCGCTTCCATTAACAGAAGCTTCTCTTGAAATGGTAAAAGCTGATATGGGCATTATGTCAATG
GCAAAATGGGCATTGCAGCCACATATCAAGAATAATTCAATTAAAGCGGTAAAAGTGGGTAAAAATGGCTTAAAACGTAA
ACATTTTATCGCCACCAGAGCAAACGAAACTTATCCTGATTATTTTCAGCATTTTATTAACTTTTTACAAAACGAAATCA
ATCTTCAATGGAATATTTAA

Upstream 100 bases:

>100_bases
AGATGTCATAAAAACGAAAAGAATTCATGAACGGCATTAGTTTTTTTCATGTAAAAGCCAGTTTAAAATTTTGTTACTTT
GTTGTAGCAATACTTTAAAA

Downstream 100 bases:

>100_bases
TTAGAAAGTGCGAAATCGCCGATTTACCTAAACTGGTTATTTTATGCCAAAAACACGCCGACTTCGAAAAGGCCGACTAT
TCTCCGGAAGGCAAAGAAGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEIRHLKLIKAIVEEGSITKAIDKLHLTQSALSHQLKEAEYQLGTPIFLRTNKKLVLTKAGEKIYELANEILNKLTETES
QIKQMVFGEYGEIRISTECFSSYHWLPSVLKQFHLLYPNVELKIVTEATHIPLQKLLENTIDIAIVSDTIKDHHIKYTEL
FQDEVVVVISENHPWAGKKYVVAEDFVNEHLIIHSLPMETVTIHQYFLAPAKVSPKKITPLPLTEASLEMVKADMGIMSM
AKWALQPHIKNNSIKAVKVGKNGLKRKHFIATRANETYPDYFQHFINFLQNEINLQWNI

Sequences:

>Translated_299_residues
MEIRHLKLIKAIVEEGSITKAIDKLHLTQSALSHQLKEAEYQLGTPIFLRTNKKLVLTKAGEKIYELANEILNKLTETES
QIKQMVFGEYGEIRISTECFSSYHWLPSVLKQFHLLYPNVELKIVTEATHIPLQKLLENTIDIAIVSDTIKDHHIKYTEL
FQDEVVVVISENHPWAGKKYVVAEDFVNEHLIIHSLPMETVTIHQYFLAPAKVSPKKITPLPLTEASLEMVKADMGIMSM
AKWALQPHIKNNSIKAVKVGKNGLKRKHFIATRANETYPDYFQHFINFLQNEINLQWNI
>Mature_299_residues
MEIRHLKLIKAIVEEGSITKAIDKLHLTQSALSHQLKEAEYQLGTPIFLRTNKKLVLTKAGEKIYELANEILNKLTETES
QIKQMVFGEYGEIRISTECFSSYHWLPSVLKQFHLLYPNVELKIVTEATHIPLQKLLENTIDIAIVSDTIKDHHIKYTEL
FQDEVVVVISENHPWAGKKYVVAEDFVNEHLIIHSLPMETVTIHQYFLAPAKVSPKKITPLPLTEASLEMVKADMGIMSM
AKWALQPHIKNNSIKAVKVGKNGLKRKHFIATRANETYPDYFQHFINFLQNEINLQWNI

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=291, Percent_Identity=30.2405498281787, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI145693105, Length=291, Percent_Identity=26.1168384879725, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI157672245, Length=277, Percent_Identity=25.2707581227437, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788887, Length=190, Percent_Identity=30.5263157894737, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787879, Length=193, Percent_Identity=25.3886010362694, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786401, Length=169, Percent_Identity=29.585798816568, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790399, Length=193, Percent_Identity=25.9067357512953, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=28.6516853932584, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788481, Length=266, Percent_Identity=24.4360902255639, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787806, Length=175, Percent_Identity=26.2857142857143, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787530, Length=311, Percent_Identity=21.8649517684887, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790208, Length=184, Percent_Identity=26.6304347826087, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1789204, Length=192, Percent_Identity=27.0833333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081904, Length=316, Percent_Identity=22.7848101265823, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=177, Percent_Identity=28.2485875706215, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=20.9876543209877, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=140, Percent_Identity=31.4285714285714, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=167, Percent_Identity=27.5449101796407, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788296, Length=155, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI2367136, Length=121, Percent_Identity=29.7520661157025, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=154, Percent_Identity=27.2727272727273, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=269, Percent_Identity=22.3048327137546, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34402; Mature: 34402

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRHLKLIKAIVEEGSITKAIDKLHLTQSALSHQLKEAEYQLGTPIFLRTNKKLVLTKA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEC
GEKIYELANEILNKLTETESQIKQMVFGEYGEIRISTECFSSYHWLPSVLKQFHLLYPNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCHHHHHHHHHHHHCCCC
ELKIVTEATHIPLQKLLENTIDIAIVSDTIKDHHIKYTELFQDEVVVVISENHPWAGKKY
EEEEEECCCCCCHHHHHHCHHEEEEEECCHHHCCCHHHHCCCCCEEEEEECCCCCCCCEE
VVAEDFVNEHLIIHSLPMETVTIHQYFLAPAKVSPKKITPLPLTEASLEMVKADMGIMSM
EEEHHHCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AKWALQPHIKNNSIKAVKVGKNGLKRKHFIATRANETYPDYFQHFINFLQNEINLQWNI
HHHHHCCCCCCCCEEEEEECCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MEIRHLKLIKAIVEEGSITKAIDKLHLTQSALSHQLKEAEYQLGTPIFLRTNKKLVLTKA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEC
GEKIYELANEILNKLTETESQIKQMVFGEYGEIRISTECFSSYHWLPSVLKQFHLLYPNV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCCHHHHHHHHHHHHCCCC
ELKIVTEATHIPLQKLLENTIDIAIVSDTIKDHHIKYTELFQDEVVVVISENHPWAGKKY
EEEEEECCCCCCHHHHHHCHHEEEEEECCHHHCCCHHHHCCCCCEEEEEECCCCCCCCEE
VVAEDFVNEHLIIHSLPMETVTIHQYFLAPAKVSPKKITPLPLTEASLEMVKADMGIMSM
EEEHHHCCCEEEEEECCCCCEEHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
AKWALQPHIKNNSIKAVKVGKNGLKRKHFIATRANETYPDYFQHFINFLQNEINLQWNI
HHHHHCCCCCCCCEEEEEECCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]