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The map label for this gene is metG

Identifier: 146298619

GI number: 146298619

Start: 967792

End: 969927

Strand: Direct

Name: metG

Synonym: Fjoh_0857

Alternate gene names: 146298619

Gene position: 967792-969927 (Clockwise)

Preceding gene: 146298617

Following gene: 146298620

Centisome position: 15.87

GC content: 37.64

Gene sequence:

>2136_bases
ATGACACAGAATCCAAAGAGATATACAATCACGGCGGCATTACCTTACACAAATGGACCAATCCACATTGGGCATTTGGC
GGGGGTTTACGTGCCTGCAGATATATATTCGAGATATTTAAGATTGCAGGGAAAAGATGTAGCATTTATTTGCGGAAGCG
ATGAACACGGCGTTGCAATTTCAATGAAAGCAAAAAAAGAAGGAGTTACGCCACAAGAAGTTATTGATAAATATGATGGA
ATTATTCGTAAATCATTTGCTGATTTCGGAATTTCATTCAACAATTACTCTAGAACTTCGGCAAAAATTCATCATGATAC
GGCTTCAGAATTCTTTAGAACTTTGTATGATAAAGGCGATTTTATTGAAGAAGTTACAGAGCAATTGTACGATGCAAAAG
CAAATCAGTTTTTAGCAGACCGTTTTGTGGTTGGAACTTGCCCAAAATGCGACAATCCGGAAGCTTATGGCGATCAGTGC
GAAAAATGCGGATCGACTTTGAACGCGACTGATTTGATCAACCCAAAATCGACAATTACAGGAGAAACTCCAATTTTAAA
AGAAACAAAACACTGGTTTTTACCTTTAGATAGATATTCTGATTTCTTAACAAAATGGATTCTTGAAGGTCACAAAAACG
ACTGGAAACCTAATGTTTACGGACAAGTAAAATCCTGGATCGACGGCGGACTTGAGCCTCGTGCTGTAACACGTGACCTT
GACTGGGGAATTGACGTTCCGGTTGAAGGTGCCGAAGGAAAAAAATTATACGTTTGGTTTGATGCACCTATTGGTTACAT
TTCTTCTACGAAAGAATGGGCAGCACGCGAAGGAAAAGACTGGGAACCATATTGGAAAGATGAAGAAACCAAATTGGTTC
ACTTTATTGGGAAAGACAATATTGTTTTTCACTGTATCATTTTTCCAGCGATGCTAAAAGCTGAAGGAAGCTACATTTTA
CCAGACAACGTTCCGGCAAATGAGTTTTTAAATTTAGAAGGAAACAAACTTTCGACTTCTAAAAACTGGGCAGTTTGGTT
ACACGAATATTTAGAAGAATTTCCAGATAAGCAGGATGTTTTACGTTACGCGTTAACATCAAACGCTCCTGAAACAAAAG
ATAACGATTTTACCTGGAAAGATTTCCAAGCTAGAAACAACAACGAATTGGTTGCTATTTTTGGAAACTTCGTAAATCGT
GTTGTGGTTTTAACCAACAAATATTACGATGGTGTTATCCCGACTCCAAACGAATTTACTGAAATCGACGAACAAACTTT
AGCTGAATTAAAAGCATATCCAGCCGTGATTTCAAGTTCGGTTGAGCGTTACAGATTCCGTGAAGCTCTGGGCGAATTGA
TGAATGTTGCTCGTTTAGGAAATAAATACCTTGCAGATGAAGAACCTTGGAAAGTAATGAAAGACAATCCAGAGCGTGTA
AAAACACAAATGTATGTAGCATTGCAAATTGCTGCAGCGTTGAGCGTTTTGGCTGAACCGTTTTTACCTTTTACAGCTGC
TAAATTGTCTAAAATCCTGAACTTAGGTGATCTTAAAGAACATTTTGAAGGGTTTAGCAAGTTCCTGAAAGAAAGACATC
AGGATGCAAACGACATCATTATTGATAAAACATTGGGTTGGAATGATATTTCTGAAAACTCAGATTTAATTCCGGCCGGA
CATAAAATTGGCGAAGCTGAATTGCTTTTCGCTAAAATAGAAGACGAAGAAATACAAAAACAAATAGATAAATTGGAAGC
GACAAAAACCGCAAATATTGCTGAAAACCAAAAAGCAGAACCACAAAAAGATTTAATTCAGTTTGAAGATTTTGCAAAAA
TGGATATTCGTATAGGAACTATTCTTGAGGCTGAAAAAATGCCAAAAGCAAACAAACTTTTGGTTCTTAAAGTAGATACG
GGAATTGATGTTCGTACAATTGTTTCGGGAATTGCAGAGAGTTTTTCGCCAGAAGAAATCATCGGAAAACGTGTTTCTGT
ATTAGCAAACCTTGCTCCAAGAGCTTTACGCGGCGTTGAAAGTCAAGGAATGATCTTGATGACAACAAATGCCGAAGGAA
AACTGGTTTTTGTAAATCCAGATGCTGATGCACCAAATGGAGCAACAGTAAACTAA

Upstream 100 bases:

>100_bases
TAGGAACGATCCATATTAAAAGTCCTTTTCGTCCTGAATCTCCATGAAACTTAAAAATCTCAATTCTATTCCTTATTTTT
GCACTCAATAAAATTGAGTT

Downstream 100 bases:

>100_bases
GAAGTTCTTTCTAGAATCGAAATATTAAAAACATGAGAAATTTCTCATGTTTTTTACTTTTATATAAGTTTATAATTGTA
AGTTTGTCTTGCAAAACATT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MTQNPKRYTITAALPYTNGPIHIGHLAGVYVPADIYSRYLRLQGKDVAFICGSDEHGVAISMKAKKEGVTPQEVIDKYDG
IIRKSFADFGISFNNYSRTSAKIHHDTASEFFRTLYDKGDFIEEVTEQLYDAKANQFLADRFVVGTCPKCDNPEAYGDQC
EKCGSTLNATDLINPKSTITGETPILKETKHWFLPLDRYSDFLTKWILEGHKNDWKPNVYGQVKSWIDGGLEPRAVTRDL
DWGIDVPVEGAEGKKLYVWFDAPIGYISSTKEWAAREGKDWEPYWKDEETKLVHFIGKDNIVFHCIIFPAMLKAEGSYIL
PDNVPANEFLNLEGNKLSTSKNWAVWLHEYLEEFPDKQDVLRYALTSNAPETKDNDFTWKDFQARNNNELVAIFGNFVNR
VVVLTNKYYDGVIPTPNEFTEIDEQTLAELKAYPAVISSSVERYRFREALGELMNVARLGNKYLADEEPWKVMKDNPERV
KTQMYVALQIAAALSVLAEPFLPFTAAKLSKILNLGDLKEHFEGFSKFLKERHQDANDIIIDKTLGWNDISENSDLIPAG
HKIGEAELLFAKIEDEEIQKQIDKLEATKTANIAENQKAEPQKDLIQFEDFAKMDIRIGTILEAEKMPKANKLLVLKVDT
GIDVRTIVSGIAESFSPEEIIGKRVSVLANLAPRALRGVESQGMILMTTNAEGKLVFVNPDADAPNGATVN

Sequences:

>Translated_711_residues
MTQNPKRYTITAALPYTNGPIHIGHLAGVYVPADIYSRYLRLQGKDVAFICGSDEHGVAISMKAKKEGVTPQEVIDKYDG
IIRKSFADFGISFNNYSRTSAKIHHDTASEFFRTLYDKGDFIEEVTEQLYDAKANQFLADRFVVGTCPKCDNPEAYGDQC
EKCGSTLNATDLINPKSTITGETPILKETKHWFLPLDRYSDFLTKWILEGHKNDWKPNVYGQVKSWIDGGLEPRAVTRDL
DWGIDVPVEGAEGKKLYVWFDAPIGYISSTKEWAAREGKDWEPYWKDEETKLVHFIGKDNIVFHCIIFPAMLKAEGSYIL
PDNVPANEFLNLEGNKLSTSKNWAVWLHEYLEEFPDKQDVLRYALTSNAPETKDNDFTWKDFQARNNNELVAIFGNFVNR
VVVLTNKYYDGVIPTPNEFTEIDEQTLAELKAYPAVISSSVERYRFREALGELMNVARLGNKYLADEEPWKVMKDNPERV
KTQMYVALQIAAALSVLAEPFLPFTAAKLSKILNLGDLKEHFEGFSKFLKERHQDANDIIIDKTLGWNDISENSDLIPAG
HKIGEAELLFAKIEDEEIQKQIDKLEATKTANIAENQKAEPQKDLIQFEDFAKMDIRIGTILEAEKMPKANKLLVLKVDT
GIDVRTIVSGIAESFSPEEIIGKRVSVLANLAPRALRGVESQGMILMTTNAEGKLVFVNPDADAPNGATVN
>Mature_710_residues
TQNPKRYTITAALPYTNGPIHIGHLAGVYVPADIYSRYLRLQGKDVAFICGSDEHGVAISMKAKKEGVTPQEVIDKYDGI
IRKSFADFGISFNNYSRTSAKIHHDTASEFFRTLYDKGDFIEEVTEQLYDAKANQFLADRFVVGTCPKCDNPEAYGDQCE
KCGSTLNATDLINPKSTITGETPILKETKHWFLPLDRYSDFLTKWILEGHKNDWKPNVYGQVKSWIDGGLEPRAVTRDLD
WGIDVPVEGAEGKKLYVWFDAPIGYISSTKEWAAREGKDWEPYWKDEETKLVHFIGKDNIVFHCIIFPAMLKAEGSYILP
DNVPANEFLNLEGNKLSTSKNWAVWLHEYLEEFPDKQDVLRYALTSNAPETKDNDFTWKDFQARNNNELVAIFGNFVNRV
VVLTNKYYDGVIPTPNEFTEIDEQTLAELKAYPAVISSSVERYRFREALGELMNVARLGNKYLADEEPWKVMKDNPERVK
TQMYVALQIAAALSVLAEPFLPFTAAKLSKILNLGDLKEHFEGFSKFLKERHQDANDIIIDKTLGWNDISENSDLIPAGH
KIGEAELLFAKIEDEEIQKQIDKLEATKTANIAENQKAEPQKDLIQFEDFAKMDIRIGTILEAEKMPKANKLLVLKVDTG
IDVRTIVSGIAESFSPEEIIGKRVSVLANLAPRALRGVESQGMILMTTNAEGKLVFVNPDADAPNGATVN

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=674, Percent_Identity=34.1246290801187, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI24308436, Length=533, Percent_Identity=25.891181988743, Blast_Score=141, Evalue=3e-33,
Organism=Homo sapiens, GI4507947, Length=99, Percent_Identity=43.4343434343434, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI215490009, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=1e-11,
Organism=Homo sapiens, GI45006986, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=1e-11,
Organism=Homo sapiens, GI215490011, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=1e-11,
Organism=Escherichia coli, GI1788432, Length=725, Percent_Identity=32.551724137931, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17540794, Length=613, Percent_Identity=35.3996737357259, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=24.4618395303327, Blast_Score=105, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71992781, Length=273, Percent_Identity=26.3736263736264, Blast_Score=70, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321704, Length=584, Percent_Identity=34.4178082191781, Blast_Score=345, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6321610, Length=536, Percent_Identity=22.9477611940299, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24655311, Length=645, Percent_Identity=35.5038759689922, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI21358483, Length=610, Percent_Identity=21.9672131147541, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21357223, Length=85, Percent_Identity=41.1764705882353, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_FLAJ1 (A5FLM7)

Other databases:

- EMBL:   CP000685
- RefSeq:   YP_001193210.1
- ProteinModelPortal:   A5FLM7
- SMR:   A5FLM7
- STRING:   A5FLM7
- GeneID:   5091713
- GenomeReviews:   CP000685_GR
- KEGG:   fjo:Fjoh_0857
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   FJOH376686:FJOH_0857-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 80195; Mature: 80063

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNPKRYTITAALPYTNGPIHIGHLAGVYVPADIYSRYLRLQGKDVAFICGSDEHGVAI
CCCCCCEEEEEEEECCCCCCEEEEECCCEEECHHHHHHHHHCCCCCEEEEECCCCCCEEE
SMKAKKEGVTPQEVIDKYDGIIRKSFADFGISFNNYSRTSAKIHHDTASEFFRTLYDKGD
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEHHHHHHHHHHHHCCCH
FIEEVTEQLYDAKANQFLADRFVVGTCPKCDNPEAYGDQCEKCGSTLNATDLINPKSTIT
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCC
GETPILKETKHWFLPLDRYSDFLTKWILEGHKNDWKPNVYGQVKSWIDGGLEPRAVTRDL
CCCCCHHCCCHHEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEECC
DWGIDVPVEGAEGKKLYVWFDAPIGYISSTKEWAAREGKDWEPYWKDEETKLVHFIGKDN
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
IVFHCIIFPAMLKAEGSYILPDNVPANEFLNLEGNKLSTSKNWAVWLHEYLEEFPDKQDV
EEEEHHHHHHHHHCCCCEECCCCCCHHHEEECCCCEECCCCCHHHHHHHHHHHCCCHHHH
LRYALTSNAPETKDNDFTWKDFQARNNNELVAIFGNFVNRVVVLTNKYYDGVIPTPNEFT
HHHHHHCCCCCCCCCCCCCCHHCCCCCCCEEEEEEHHHCEEEEEEECCCCCCCCCCCHHH
EIDEQTLAELKAYPAVISSSVERYRFREALGELMNVARLGNKYLADEEPWKVMKDNPERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHH
KTQMYVALQIAAALSVLAEPFLPFTAAKLSKILNLGDLKEHFEGFSKFLKERHQDANDII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEE
IDKTLGWNDISENSDLIPAGHKIGEAELLFAKIEDEEIQKQIDKLEATKTANIAENQKAE
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
PQKDLIQFEDFAKMDIRIGTILEAEKMPKANKLLVLKVDTGIDVRTIVSGIAESFSPEEI
CHHHHHHHHHHHHHEEEEEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHH
IGKRVSVLANLAPRALRGVESQGMILMTTNAEGKLVFVNPDADAPNGATVN
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TQNPKRYTITAALPYTNGPIHIGHLAGVYVPADIYSRYLRLQGKDVAFICGSDEHGVAI
CCCCCEEEEEEEECCCCCCEEEEECCCEEECHHHHHHHHHCCCCCEEEEECCCCCCEEE
SMKAKKEGVTPQEVIDKYDGIIRKSFADFGISFNNYSRTSAKIHHDTASEFFRTLYDKGD
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCEEEHHHHHHHHHHHHCCCH
FIEEVTEQLYDAKANQFLADRFVVGTCPKCDNPEAYGDQCEKCGSTLNATDLINPKSTIT
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCC
GETPILKETKHWFLPLDRYSDFLTKWILEGHKNDWKPNVYGQVKSWIDGGLEPRAVTRDL
CCCCCHHCCCHHEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCEEECC
DWGIDVPVEGAEGKKLYVWFDAPIGYISSTKEWAAREGKDWEPYWKDEETKLVHFIGKDN
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
IVFHCIIFPAMLKAEGSYILPDNVPANEFLNLEGNKLSTSKNWAVWLHEYLEEFPDKQDV
EEEEHHHHHHHHHCCCCEECCCCCCHHHEEECCCCEECCCCCHHHHHHHHHHHCCCHHHH
LRYALTSNAPETKDNDFTWKDFQARNNNELVAIFGNFVNRVVVLTNKYYDGVIPTPNEFT
HHHHHHCCCCCCCCCCCCCCHHCCCCCCCEEEEEEHHHCEEEEEEECCCCCCCCCCCHHH
EIDEQTLAELKAYPAVISSSVERYRFREALGELMNVARLGNKYLADEEPWKVMKDNPERV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHH
KTQMYVALQIAAALSVLAEPFLPFTAAKLSKILNLGDLKEHFEGFSKFLKERHQDANDII
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEE
IDKTLGWNDISENSDLIPAGHKIGEAELLFAKIEDEEIQKQIDKLEATKTANIAENQKAE
EECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
PQKDLIQFEDFAKMDIRIGTILEAEKMPKANKLLVLKVDTGIDVRTIVSGIAESFSPEEI
CHHHHHHHHHHHHHEEEEEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHH
IGKRVSVLANLAPRALRGVESQGMILMTTNAEGKLVFVNPDADAPNGATVN
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA