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The map label for this gene is radA [H]

Identifier: 146298590

GI number: 146298590

Start: 938779

End: 940140

Strand: Direct

Name: radA [H]

Synonym: Fjoh_0828

Alternate gene names: 146298590

Gene position: 938779-940140 (Clockwise)

Preceding gene: 146298589

Following gene: 146298591

Centisome position: 15.4

GC content: 38.69

Gene sequence:

>1362_bases
ATGTCAAAAGTTAAAACTTCCTTTTTTTGCCAAAATTGCGGCACCCAATATTCTAAATGGCAGGGGCAGTGCAACGCGTG
CAAGGAATGGAATACAATTGCTGAAGAAATAATCCAGAAACAAGAAAAAGTCGCCTGGAAAAGCGAGCCGACTTCATCAA
GTAAAGCACCAAGACCTTTAAAAATAACTGAAATTGATTCGGCGCAGGAAATTCGTATGGATACTACCGACGGCGAATTG
AATCGTGTTTTGGGAGGCGGAATTGTTCCGGGATCTTTAACACTTTTAGGCGGCGAACCAGGAATTGGAAAAAGTACACT
TTTACTTCAAATCTCATTAAAATTACCTTATAAAACCTTATATGTTTCTGGCGAAGAAAGCCAGAAACAAATAAAAATGC
GTGCCGAAAGAATAACGCCAAACAGCGATAACTGCTATATTTTAACGGAAACCAAAACGCAAAACATTTTTAAACAAATT
GAAGCCATTCAGCCTGAAATTGTAATCATCGATTCGATTCAGACTTTACATACAGATTATATTGAATCTACTGCCGGCAG
TATTTCTCAAATTAGAGAAACCACTGCCGAATTGATCAAATTTGCTAAAGAAACCAATATTCCGGTTATTTTAATTGGTC
ATATTACTAAAGACGGAAATATCGCCGGACCAAAAATTCTGGAACACATGGTTGACACCGTTCTTCAGTTTGAAGGCGAC
AGAAACCATATTTATCGAATTCTGCGTTCGCTGAAAAATCGCTTTGGTTCAACCTCTGAACTGGGAATTTACGAAATGCT
TGGAAGCGGTTTGCGAGAAGTCAGCAATCCGTCAGAAATATTAATTTCACACAAAGACGAAGAATTATCAGGAACTGCAA
TTGCCACAACTCTTGAGGGAATGCGCCCGCTGATGATCGAAATTCAGTCTTTGGTAAGTACCGCTGTTTATGGAACTCCT
CAAAGAAGCACAACAGGTTACAATGCCAAACGTCTAAACATGATTTTGGCTGTTTTAGAAAAAAGAGCCGGATTCCGTTT
GGGTGCAAAAGACGTTTTCTTAAATGTAACCGGAGGAATTTCTGTAGATGATCCTGCGATTGATCTTGCAGTTGTTGCTG
CAATTTTATCATCAAATGAAGATATACCCGTTGGAAAAGGTTTTTGTTTTGCCGGTGAAGTTGGTCTTTCTGGAGAAATT
CGTCCTGTAAACCGTGTAGACCAGCGCATTCAGGAAGCCGAAAAATTAGGCTTCGACACGATCTTTGTTTCTAAATACAA
TAAAATTGCTTTAAAAAATACAGGAATCAAAGTTGAACTTGTAGCAAAAATTGAAGATGTTGCCAGTATCCTTTTTGGTT
GA

Upstream 100 bases:

>100_bases
ACCAGAATGCTTCTCAAATGGAGCCAATTGGAGATTTAAACAAAGATTTGATGTATGAGAAAATCGACGAAATGAATACG
CTTGCTAAAAAAAGTAAATA

Downstream 100 bases:

>100_bases
TCTCTGATAAAAATACCTCTATGAATTTTCCTAAACAAAAAATATACAAAGCTTTAAAAATACTTGCCGTAGTATTTATA
TTCCTTTGCATTGGTCTGTA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSKVKTSFFCQNCGTQYSKWQGQCNACKEWNTIAEEIIQKQEKVAWKSEPTSSSKAPRPLKITEIDSAQEIRMDTTDGEL
NRVLGGGIVPGSLTLLGGEPGIGKSTLLLQISLKLPYKTLYVSGEESQKQIKMRAERITPNSDNCYILTETKTQNIFKQI
EAIQPEIVIIDSIQTLHTDYIESTAGSISQIRETTAELIKFAKETNIPVILIGHITKDGNIAGPKILEHMVDTVLQFEGD
RNHIYRILRSLKNRFGSTSELGIYEMLGSGLREVSNPSEILISHKDEELSGTAIATTLEGMRPLMIEIQSLVSTAVYGTP
QRSTTGYNAKRLNMILAVLEKRAGFRLGAKDVFLNVTGGISVDDPAIDLAVVAAILSSNEDIPVGKGFCFAGEVGLSGEI
RPVNRVDQRIQEAEKLGFDTIFVSKYNKIALKNTGIKVELVAKIEDVASILFG

Sequences:

>Translated_453_residues
MSKVKTSFFCQNCGTQYSKWQGQCNACKEWNTIAEEIIQKQEKVAWKSEPTSSSKAPRPLKITEIDSAQEIRMDTTDGEL
NRVLGGGIVPGSLTLLGGEPGIGKSTLLLQISLKLPYKTLYVSGEESQKQIKMRAERITPNSDNCYILTETKTQNIFKQI
EAIQPEIVIIDSIQTLHTDYIESTAGSISQIRETTAELIKFAKETNIPVILIGHITKDGNIAGPKILEHMVDTVLQFEGD
RNHIYRILRSLKNRFGSTSELGIYEMLGSGLREVSNPSEILISHKDEELSGTAIATTLEGMRPLMIEIQSLVSTAVYGTP
QRSTTGYNAKRLNMILAVLEKRAGFRLGAKDVFLNVTGGISVDDPAIDLAVVAAILSSNEDIPVGKGFCFAGEVGLSGEI
RPVNRVDQRIQEAEKLGFDTIFVSKYNKIALKNTGIKVELVAKIEDVASILFG
>Mature_452_residues
SKVKTSFFCQNCGTQYSKWQGQCNACKEWNTIAEEIIQKQEKVAWKSEPTSSSKAPRPLKITEIDSAQEIRMDTTDGELN
RVLGGGIVPGSLTLLGGEPGIGKSTLLLQISLKLPYKTLYVSGEESQKQIKMRAERITPNSDNCYILTETKTQNIFKQIE
AIQPEIVIIDSIQTLHTDYIESTAGSISQIRETTAELIKFAKETNIPVILIGHITKDGNIAGPKILEHMVDTVLQFEGDR
NHIYRILRSLKNRFGSTSELGIYEMLGSGLREVSNPSEILISHKDEELSGTAIATTLEGMRPLMIEIQSLVSTAVYGTPQ
RSTTGYNAKRLNMILAVLEKRAGFRLGAKDVFLNVTGGISVDDPAIDLAVVAAILSSNEDIPVGKGFCFAGEVGLSGEIR
PVNRVDQRIQEAEKLGFDTIFVSKYNKIALKNTGIKVELVAKIEDVASILFG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=452, Percent_Identity=43.8053097345133, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49742; Mature: 49611

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKTSFFCQNCGTQYSKWQGQCNACKEWNTIAEEIIQKQEKVAWKSEPTSSSKAPRPL
CCCCCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
KITEIDSAQEIRMDTTDGELNRVLGGGIVPGSLTLLGGEPGIGKSTLLLQISLKLPYKTL
EEEECCCHHHEEEECCCCHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEE
YVSGEESQKQIKMRAERITPNSDNCYILTETKTQNIFKQIEAIQPEIVIIDSIQTLHTDY
EECCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHH
IESTAGSISQIRETTAELIKFAKETNIPVILIGHITKDGNIAGPKILEHMVDTVLQFEGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
RNHIYRILRSLKNRFGSTSELGIYEMLGSGLREVSNPSEILISHKDEELSGTAIATTLEG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHC
MRPLMIEIQSLVSTAVYGTPQRSTTGYNAKRLNMILAVLEKRAGFRLGAKDVFLNVTGGI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEEECCC
SVDDPAIDLAVVAAILSSNEDIPVGKGFCFAGEVGLSGEIRPVNRVDQRIQEAEKLGFDT
CCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCE
IFVSKYNKIALKNTGIKVELVAKIEDVASILFG
EEEECCCEEEEECCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVKTSFFCQNCGTQYSKWQGQCNACKEWNTIAEEIIQKQEKVAWKSEPTSSSKAPRPL
CCCCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
KITEIDSAQEIRMDTTDGELNRVLGGGIVPGSLTLLGGEPGIGKSTLLLQISLKLPYKTL
EEEECCCHHHEEEECCCCHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEE
YVSGEESQKQIKMRAERITPNSDNCYILTETKTQNIFKQIEAIQPEIVIIDSIQTLHTDY
EECCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECHHHHHHHH
IESTAGSISQIRETTAELIKFAKETNIPVILIGHITKDGNIAGPKILEHMVDTVLQFEGD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
RNHIYRILRSLKNRFGSTSELGIYEMLGSGLREVSNPSEILISHKDEELSGTAIATTLEG
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHC
MRPLMIEIQSLVSTAVYGTPQRSTTGYNAKRLNMILAVLEKRAGFRLGAKDVFLNVTGGI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEEECCC
SVDDPAIDLAVVAAILSSNEDIPVGKGFCFAGEVGLSGEIRPVNRVDQRIQEAEKLGFDT
CCCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCE
IFVSKYNKIALKNTGIKVELVAKIEDVASILFG
EEEECCCEEEEECCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]