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The map label for this gene is mhpC [H]

Identifier: 146298456

GI number: 146298456

Start: 758719

End: 759495

Strand: Direct

Name: mhpC [H]

Synonym: Fjoh_0693

Alternate gene names: 146298456

Gene position: 758719-759495 (Clockwise)

Preceding gene: 146298455

Following gene: 146298464

Centisome position: 12.44

GC content: 33.98

Gene sequence:

>777_bases
TTGAAAACGCTTTTATATAAAAACACCACGATTTCATACTCAGATTCAGGAACTGGAAACGCAATTGTTTTACTGCACGG
CTTTTTGGAAAACAAAAAAATGTGGAAAGACTATGTTGCTTTTTTTTCAGAAAAATATCGCGTCATAACAATCGATTTAT
TAGGTCATGGCGAATCTGATCCATTAGGCTATGTTCATGAAATGGAAGATAATGCCAATGTTATAAACGAAATTTTAGAA
CATTTAAAAATAGAAAAAGCGATAATTCTTGGGCATTCAATGGGCGGATATGTTGGTTTGGCTTTCGCCGAATTGTATCC
GCAGAAAATACAGAAATTGGTTTTACTAAATTCAACTTCAAAAGAAGACAGCGCCGAAAAAAAATTAAACAGAACCCGGG
CCATAAAAGCTGTAAAACAAAACTATATCAACTTTGTCAGCTTAGCCATTGCTAATTTATTCAGCGAAAACAATAGAACG
CGATTAGCAGAAGAAATTGAAAAGGCAAAAATAGAAGCTTTAAAAACTCCTTTGCAGGGAATTATAGCTTCATTGGAAGG
AATGAAAATACGAAAAGACCGAGAGTGGCTGTTAGAACAAAACCGCTTTCCTGTTTTATTAATTTTAGGAAAAAAAGATC
CTGTTTTAAGCTACGAAGAAAGTCTTTCGCAAGCAGAGAATACATCTGTACAATTAGTTTCTTTTGAAGACGGGCATATG
AGCCAAATTGAAAACAAAGAAGAATTGAAGCCTGTTTTATTACAGTTCTTCAACTAA

Upstream 100 bases:

>100_bases
AATTTATTACTACTTTAAAGATTTCCCAATTAAACCAAGACATTTCGATATTTTACTCCAGCTTTCTTAACTTTGTGCTT
CATAACAAAAAAAGTTCATT

Downstream 100 bases:

>100_bases
AATTAAAGGAAAATATTAAAAGCAGAAAAGGCCATTTCGAAATGATATCGAAACAGCCTTTTCTTCATTTTGGGGGCAAA
AATTATAATTTTGTAATAAT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKTLLYKNTTISYSDSGTGNAIVLLHGFLENKKMWKDYVAFFSEKYRVITIDLLGHGESDPLGYVHEMEDNANVINEILE
HLKIEKAIILGHSMGGYVGLAFAELYPQKIQKLVLLNSTSKEDSAEKKLNRTRAIKAVKQNYINFVSLAIANLFSENNRT
RLAEEIEKAKIEALKTPLQGIIASLEGMKIRKDREWLLEQNRFPVLLILGKKDPVLSYEESLSQAENTSVQLVSFEDGHM
SQIENKEELKPVLLQFFN

Sequences:

>Translated_258_residues
MKTLLYKNTTISYSDSGTGNAIVLLHGFLENKKMWKDYVAFFSEKYRVITIDLLGHGESDPLGYVHEMEDNANVINEILE
HLKIEKAIILGHSMGGYVGLAFAELYPQKIQKLVLLNSTSKEDSAEKKLNRTRAIKAVKQNYINFVSLAIANLFSENNRT
RLAEEIEKAKIEALKTPLQGIIASLEGMKIRKDREWLLEQNRFPVLLILGKKDPVLSYEESLSQAENTSVQLVSFEDGHM
SQIENKEELKPVLLQFFN
>Mature_258_residues
MKTLLYKNTTISYSDSGTGNAIVLLHGFLENKKMWKDYVAFFSEKYRVITIDLLGHGESDPLGYVHEMEDNANVINEILE
HLKIEKAIILGHSMGGYVGLAFAELYPQKIQKLVLLNSTSKEDSAEKKLNRTRAIKAVKQNYINFVSLAIANLFSENNRT
RLAEEIEKAKIEALKTPLQGIIASLEGMKIRKDREWLLEQNRFPVLLILGKKDPVLSYEESLSQAENTSVQLVSFEDGHM
SQIENKEELKPVLLQFFN

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mhpC alpha/beta hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=143, Percent_Identity=28.6713286713287, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI87081721, Length=265, Percent_Identity=26.0377358490566, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554012, Length=115, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29348; Mature: 29348

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLYKNTTISYSDSGTGNAIVLLHGFLENKKMWKDYVAFFSEKYRVITIDLLGHGESD
CCCEEEECCEEEECCCCCCCEEEEEEEHHHCHHHHHHHHHHHHCCCEEEEEEEECCCCCC
PLGYVHEMEDNANVINEILEHLKIEKAIILGHSMGGYVGLAFAELYPQKIQKLVLLNSTS
CCCHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
KEDSAEKKLNRTRAIKAVKQNYINFVSLAIANLFSENNRTRLAEEIEKAKIEALKTPLQG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IIASLEGMKIRKDREWLLEQNRFPVLLILGKKDPVLSYEESLSQAENTSVQLVSFEDGHM
HHHHHCCCEECCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCH
SQIENKEELKPVLLQFFN
HHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLLYKNTTISYSDSGTGNAIVLLHGFLENKKMWKDYVAFFSEKYRVITIDLLGHGESD
CCCEEEECCEEEECCCCCCCEEEEEEEHHHCHHHHHHHHHHHHCCCEEEEEEEECCCCCC
PLGYVHEMEDNANVINEILEHLKIEKAIILGHSMGGYVGLAFAELYPQKIQKLVLLNSTS
CCCHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCC
KEDSAEKKLNRTRAIKAVKQNYINFVSLAIANLFSENNRTRLAEEIEKAKIEALKTPLQG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
IIASLEGMKIRKDREWLLEQNRFPVLLILGKKDPVLSYEESLSQAENTSVQLVSFEDGHM
HHHHHCCCEECCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEEEEECCCCH
SQIENKEELKPVLLQFFN
HHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA