Click here to switch to the map view.

The map label for this gene is 146298441

Identifier: 146298441

GI number: 146298441

Start: 743902

End: 745263

Strand: Direct

Name: 146298441

Synonym: Fjoh_0678

Alternate gene names: NA

Gene position: 743902-745263 (Clockwise)

Preceding gene: 146298437

Following gene: 146298443

Centisome position: 12.2

GC content: 34.95

Gene sequence:

>1362_bases
ATGAAAACAGGAATTGATGCTATATCTTTTGACGTAGCAAACATACATTTACCCATAAAAACTTTGGCCATTGCCAGAAA
TATTGAACCAGAAAAATTAGAAAAAGGTCTTGGATTACTAAAAATGACTTTCCCGGACGTTCATCAGGACGCTGTTGTTT
TTGGAGCAAATGCTTTAACCAAGCTTATCATTGATAATAAAATTGACTTAAAAGAAATAAGCCGAATTTATGTTGGTACC
GAAAGCAGCATTGACAGCTCTAAACCAATTGCTTCTTATTTAATTAGTTTAATGGAGCAAAAATTTGGCGAAGATTCACT
AGCAGAATGTGATGTTGTAGATTTTACTTTTGCCTGCATTGGCGGAGTTGACGCGATGCAAAACTGTCTTGATTTCGTAA
AATTAAATCCAGCAAAAAAAGCAATTGTAGTTACTTCTGATTTTGCAAAATACGACTTAAACTCTGGCGGAGAATATACG
CAAGGTGCCGGAGCAGTTGCAATGCTGATTACCGCAGACCCAAAAATTATTGCTTTTGATGACAATTGGGCAACAAGCAC
AAAAGGCGTTTTCGATTTCTTTAAACCATACAGAACTATCTCTAAAGAAGAAATTACAAAAAATACCAGCAACGACCCTT
GGTTTGACAATTTAGAAGCCGAAATTGAAATCCATAAAGACCAGCCGGTTTTTGACGGACAATATTCTAACCAATGTTAT
ATGGATCGTACGCGCAATGCCTACTTTTCATTCAAAAAATTAAAAAACACTACCGAAACTCTTTATAACACTTGGCACAG
TATCGTAATGCACCTGCCATATTCTTTCCAGGGAAGAAGAATGTTATCTGAAATCTACGCTTTAGACAGTGCCGAAAAAA
TTATCGCTGATGATATTGCACCAGCCGATTATCAGACAAAAATTAAAGAAGTGGCAAAATCTGATGATTACAGAAGTTTT
GTAACCGAGAAATTACAACCTGCAGAATTGGCTTCTTCTTTAATAGGAAACCTTTACACTGGTTCTATTTTCATGGGATT
GTTATCGACTTTGGCTCATTTTTATGATACAAAAAAAGAAGTTGCAGGAACTAAATTTGGTTTCCTTGCTTATGGAAGCG
GATCAAAATCGAAAGTTTTTGAAGGAACGATTCAGCCAGAATGGAAATCAGCTTTAGAAAACGTAAAGCTTTTTGAAAAT
TTAACCGAAAGCACAGAAATTGATTTCAACACTTACGAAAGCCTTCATAAAAAAGAACAAAAACAAAGTATAAGAACTCC
AAAAAACGAATGGATTTTAGACAGAATCGAAAAAGAGATTCCTGTTTTAATTGGAGCTCGTTATTATAAATGGATTGATT
AA

Upstream 100 bases:

>100_bases
TTTGTTTTCTTCAAAAGTGTTCTATGTCCGGAATTTTATCAAAGTAAAAAATTGTCATTCCGCAACATAGTTTTACCTTT
AGCGGCTCAAAAAAATAAGA

Downstream 100 bases:

>100_bases
GAAATTCCAATAAAAAAACCGAAGCAAATGCTTCGGTTTTTTTATAACACAAAGTTTGTCATTGCGAGGAACGAAGCAAC
CACACTAACAATAATAAACG

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKTGIDAISFDVANIHLPIKTLAIARNIEPEKLEKGLGLLKMTFPDVHQDAVVFGANALTKLIIDNKIDLKEISRIYVGT
ESSIDSSKPIASYLISLMEQKFGEDSLAECDVVDFTFACIGGVDAMQNCLDFVKLNPAKKAIVVTSDFAKYDLNSGGEYT
QGAGAVAMLITADPKIIAFDDNWATSTKGVFDFFKPYRTISKEEITKNTSNDPWFDNLEAEIEIHKDQPVFDGQYSNQCY
MDRTRNAYFSFKKLKNTTETLYNTWHSIVMHLPYSFQGRRMLSEIYALDSAEKIIADDIAPADYQTKIKEVAKSDDYRSF
VTEKLQPAELASSLIGNLYTGSIFMGLLSTLAHFYDTKKEVAGTKFGFLAYGSGSKSKVFEGTIQPEWKSALENVKLFEN
LTESTEIDFNTYESLHKKEQKQSIRTPKNEWILDRIEKEIPVLIGARYYKWID

Sequences:

>Translated_453_residues
MKTGIDAISFDVANIHLPIKTLAIARNIEPEKLEKGLGLLKMTFPDVHQDAVVFGANALTKLIIDNKIDLKEISRIYVGT
ESSIDSSKPIASYLISLMEQKFGEDSLAECDVVDFTFACIGGVDAMQNCLDFVKLNPAKKAIVVTSDFAKYDLNSGGEYT
QGAGAVAMLITADPKIIAFDDNWATSTKGVFDFFKPYRTISKEEITKNTSNDPWFDNLEAEIEIHKDQPVFDGQYSNQCY
MDRTRNAYFSFKKLKNTTETLYNTWHSIVMHLPYSFQGRRMLSEIYALDSAEKIIADDIAPADYQTKIKEVAKSDDYRSF
VTEKLQPAELASSLIGNLYTGSIFMGLLSTLAHFYDTKKEVAGTKFGFLAYGSGSKSKVFEGTIQPEWKSALENVKLFEN
LTESTEIDFNTYESLHKKEQKQSIRTPKNEWILDRIEKEIPVLIGARYYKWID
>Mature_453_residues
MKTGIDAISFDVANIHLPIKTLAIARNIEPEKLEKGLGLLKMTFPDVHQDAVVFGANALTKLIIDNKIDLKEISRIYVGT
ESSIDSSKPIASYLISLMEQKFGEDSLAECDVVDFTFACIGGVDAMQNCLDFVKLNPAKKAIVVTSDFAKYDLNSGGEYT
QGAGAVAMLITADPKIIAFDDNWATSTKGVFDFFKPYRTISKEEITKNTSNDPWFDNLEAEIEIHKDQPVFDGQYSNQCY
MDRTRNAYFSFKKLKNTTETLYNTWHSIVMHLPYSFQGRRMLSEIYALDSAEKIIADDIAPADYQTKIKEVAKSDDYRSF
VTEKLQPAELASSLIGNLYTGSIFMGLLSTLAHFYDTKKEVAGTKFGFLAYGSGSKSKVFEGTIQPEWKSALENVKLFEN
LTESTEIDFNTYESLHKKEQKQSIRTPKNEWILDRIEKEIPVLIGARYYKWID

Specific function: Unknown

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0219 family [H]

Homologues:

Organism=Homo sapiens, GI148298764, Length=376, Percent_Identity=25, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI148298677, Length=376, Percent_Identity=25, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI5031751, Length=392, Percent_Identity=25, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17560126, Length=439, Percent_Identity=28.0182232346241, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323509, Length=396, Percent_Identity=26.7676767676768, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24654139, Length=367, Percent_Identity=25.6130790190736, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24654137, Length=367, Percent_Identity=25.6130790190736, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24654133, Length=367, Percent_Identity=25.6130790190736, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24654135, Length=367, Percent_Identity=25.6130790190736, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17933694, Length=367, Percent_Identity=25.6130790190736, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013747
- InterPro:   IPR004656
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08541 ACP_syn_III_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 51109; Mature: 51109

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTGIDAISFDVANIHLPIKTLAIARNIEPEKLEKGLGLLKMTFPDVHQDAVVFGANALT
CCCCCCEEEEEEEEEEEHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCCCEEECHHHHH
KLIIDNKIDLKEISRIYVGTESSIDSSKPIASYLISLMEQKFGEDSLAECDVVDFTFACI
HHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GGVDAMQNCLDFVKLNPAKKAIVVTSDFAKYDLNSGGEYTQGAGAVAMLITADPKIIAFD
CCHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEC
DNWATSTKGVFDFFKPYRTISKEEITKNTSNDPWFDNLEAEIEIHKDQPVFDGQYSNQCY
CCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCH
MDRTRNAYFSFKKLKNTTETLYNTWHSIVMHLPYSFQGRRMLSEIYALDSAEKIIADDIA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHCCC
PADYQTKIKEVAKSDDYRSFVTEKLQPAELASSLIGNLYTGSIFMGLLSTLAHFYDTKKE
CCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGTKFGFLAYGSGSKSKVFEGTIQPEWKSALENVKLFENLTESTEIDFNTYESLHKKEQ
HCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KQSIRTPKNEWILDRIEKEIPVLIGARYYKWID
HHHHCCCCHHHHHHHHHHHCCEEECCHHHHCCC
>Mature Secondary Structure
MKTGIDAISFDVANIHLPIKTLAIARNIEPEKLEKGLGLLKMTFPDVHQDAVVFGANALT
CCCCCCEEEEEEEEEEEHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCCCEEECHHHHH
KLIIDNKIDLKEISRIYVGTESSIDSSKPIASYLISLMEQKFGEDSLAECDVVDFTFACI
HHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GGVDAMQNCLDFVKLNPAKKAIVVTSDFAKYDLNSGGEYTQGAGAVAMLITADPKIIAFD
CCHHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEEC
DNWATSTKGVFDFFKPYRTISKEEITKNTSNDPWFDNLEAEIEIHKDQPVFDGQYSNQCY
CCCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCH
MDRTRNAYFSFKKLKNTTETLYNTWHSIVMHLPYSFQGRRMLSEIYALDSAEKIIADDIA
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHCCC
PADYQTKIKEVAKSDDYRSFVTEKLQPAELASSLIGNLYTGSIFMGLLSTLAHFYDTKKE
CCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAGTKFGFLAYGSGSKSKVFEGTIQPEWKSALENVKLFENLTESTEIDFNTYESLHKKEQ
HCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KQSIRTPKNEWILDRIEKEIPVLIGARYYKWID
HHHHCCCCHHHHHHHHHHHCCEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA