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The map label for this gene is pulD [H]

Identifier: 146298381

GI number: 146298381

Start: 679808

End: 681712

Strand: Direct

Name: pulD [H]

Synonym: Fjoh_0618

Alternate gene names: 146298381

Gene position: 679808-681712 (Clockwise)

Preceding gene: 146298380

Following gene: 146298382

Centisome position: 11.15

GC content: 32.49

Gene sequence:

>1905_bases
ATGTTTAAAAAATTAGGTTACGTATTTATCATACTGTTTTTTACAAATAGTATTATTGCTCAGCAAGACATAAATCAATT
AAGCAAACAATTTGATGCTCTTTCTGTACAGAAAAAAGGTCTTAATGAAACTATTAAAATTGATGTATCCGGATTAACAT
TACGAGATTTCATTTCTTCTATTGCCGAAGAACACCAGCTGAATATTGATGTCGATTCAGAATTAACCCAGCAGGTATCC
AACAACTTTTTTGATGTAACAGTAAAAGATGTATTGATTCATTTGGTTCAAAAATATGATCTGGAAGTAACTTTCATGAA
CAACATCATTGTTTTCAAAAAAAGAAAAGCAGTAACTGTTGTAGAGAAAAAAGCACCAAAAGTAATTGATGTCAGTTACA
ATCCTCAAAATGATTTTTTGTCTGTAAAACTAGAAAACGATACACTTTCGGCTGTTGCAAAAGCTATCATCGATAAATCG
GGTAAAAACTTAATTGTGGCGGCCGATGCAAAAAATCTTCGAGTTTCCTCTTATATTCTAAATCGTCCGTTTGATCAGGT
TATAGAAATGATGTCAAAATCAAATGATCTGACAGCAACAAAAGATGATAATGGCTTTTTCTATATTGAAAAAAATAAAG
ATGCGACAGTAACTGTAAATGGAAGCACGCAAAAAGTGAAACCGCCAAAAGCAAGTTCTGGACTTCCTGGATATTATGAA
GTTAATTTAAATAAAAATGGCTTTCTGTCTGTAAAAGCATATATAGCAGACGCCTCAGATGTATTAACAGAAGCTGCCGA
AAAACTGCACGTAAACTATTTTTTATACAACAAACCCGAGAATGAAAAAACTACTCTGTCTGCTGATAATATAACCTTCG
ATGAGTTGTTGTTTCACATTTTTAAAGGAAAAAAATATACATACAAAAAACAGGATAATTTATATTTAATTGGTGAAGAA
GGTTCTGAGGGATTAAGAGCTACAGAGGTAATTCAGCTTGAAAACAGATCTATTGAATTGGTTCTTCCATCGCTTCCAAA
AGTGTTTTCTGAAAAACTTGAAATTAAAGAGTTTGTCGAACTAAACGGACTTGTAGCTTCTGGTTCAAAATCTATTCTTG
AGGAATTAAAACTTTATATAAAGCAAATTGATAAGATTGTACCAATGGTGCAGATTGAAGTCATAATCGCTCAATATAAC
AAATCTCACGATGTACAAACCGGAATGAAAGCAGGACTTGACAAAATCAACAAACAAGTTACAGGCGGCACTGTGTTTCC
TACCACAGATATGACTGTAAATTCAACTTCTGTAAATAGTTTAATTGATGCATTCAATGGATTCGGAATTTTTAAACTAG
GAAAAGTTACAGAGGCTTTCTACCTAAACTTAAAATTACTCGAAAACAATTCAATTGTAAAAGTAGAATCAACTCCTAAG
ATTGCAACAATAAGCGGACATGAAGCTAAATTATCTATTGGAGAAACAAGTTACTATTTTGAACAAAGCAACCGTTTAAT
TAACAGTAATATTGGTAACGATATCTTGAATTCGGGAACATGGAAATCTACTGATGCTAATTTAAGTCTCTCTATAAAGC
CTTATGTTTCTATTGATGAAAATGTGACTTTGACAATTGTTGTCGAAAAAAGTTCATTCTTGGGAAGAGCCGGCGAGACC
GCTCCTCCTGGAAAAGCAACACAAAAATTTGAATCATTGGTACGTGTAAAAAATGGAGAAATGATTTTGCTAGGCGGTTT
AGACGAATTAAAGAAAGAAGATTCTGGCTCAGGAACTCCCCTATTATCAAGAATTCCAATTATCAAATGGTTTTTTAGCA
GCCGTAAAAAAGGGAAAAACAATTCAAAACTGCATATTTTTATTAAACCAACAGTGGTATATTAA

Upstream 100 bases:

>100_bases
GAAAACTATAAATCATTATCAGAAAAAGCTTTTGATATCCTTGCAGAAGGAGAAACAGCTTTAGAAGAAATATATTCTAT
TTTAATTAACTTATAACTAA

Downstream 100 bases:

>100_bases
TGATTACATTTTTATCTAAAATAATTAAACTAAACAATTTACATGTAATTGGAATCATCAGAAATGAAGATGAAGAAATT
TACAATGTACTTACTGTTAA

Product: type II and III secretion system protein

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MFKKLGYVFIILFFTNSIIAQQDINQLSKQFDALSVQKKGLNETIKIDVSGLTLRDFISSIAEEHQLNIDVDSELTQQVS
NNFFDVTVKDVLIHLVQKYDLEVTFMNNIIVFKKRKAVTVVEKKAPKVIDVSYNPQNDFLSVKLENDTLSAVAKAIIDKS
GKNLIVAADAKNLRVSSYILNRPFDQVIEMMSKSNDLTATKDDNGFFYIEKNKDATVTVNGSTQKVKPPKASSGLPGYYE
VNLNKNGFLSVKAYIADASDVLTEAAEKLHVNYFLYNKPENEKTTLSADNITFDELLFHIFKGKKYTYKKQDNLYLIGEE
GSEGLRATEVIQLENRSIELVLPSLPKVFSEKLEIKEFVELNGLVASGSKSILEELKLYIKQIDKIVPMVQIEVIIAQYN
KSHDVQTGMKAGLDKINKQVTGGTVFPTTDMTVNSTSVNSLIDAFNGFGIFKLGKVTEAFYLNLKLLENNSIVKVESTPK
IATISGHEAKLSIGETSYYFEQSNRLINSNIGNDILNSGTWKSTDANLSLSIKPYVSIDENVTLTIVVEKSSFLGRAGET
APPGKATQKFESLVRVKNGEMILLGGLDELKKEDSGSGTPLLSRIPIIKWFFSSRKKGKNNSKLHIFIKPTVVY

Sequences:

>Translated_634_residues
MFKKLGYVFIILFFTNSIIAQQDINQLSKQFDALSVQKKGLNETIKIDVSGLTLRDFISSIAEEHQLNIDVDSELTQQVS
NNFFDVTVKDVLIHLVQKYDLEVTFMNNIIVFKKRKAVTVVEKKAPKVIDVSYNPQNDFLSVKLENDTLSAVAKAIIDKS
GKNLIVAADAKNLRVSSYILNRPFDQVIEMMSKSNDLTATKDDNGFFYIEKNKDATVTVNGSTQKVKPPKASSGLPGYYE
VNLNKNGFLSVKAYIADASDVLTEAAEKLHVNYFLYNKPENEKTTLSADNITFDELLFHIFKGKKYTYKKQDNLYLIGEE
GSEGLRATEVIQLENRSIELVLPSLPKVFSEKLEIKEFVELNGLVASGSKSILEELKLYIKQIDKIVPMVQIEVIIAQYN
KSHDVQTGMKAGLDKINKQVTGGTVFPTTDMTVNSTSVNSLIDAFNGFGIFKLGKVTEAFYLNLKLLENNSIVKVESTPK
IATISGHEAKLSIGETSYYFEQSNRLINSNIGNDILNSGTWKSTDANLSLSIKPYVSIDENVTLTIVVEKSSFLGRAGET
APPGKATQKFESLVRVKNGEMILLGGLDELKKEDSGSGTPLLSRIPIIKWFFSSRKKGKNNSKLHIFIKPTVVY
>Mature_634_residues
MFKKLGYVFIILFFTNSIIAQQDINQLSKQFDALSVQKKGLNETIKIDVSGLTLRDFISSIAEEHQLNIDVDSELTQQVS
NNFFDVTVKDVLIHLVQKYDLEVTFMNNIIVFKKRKAVTVVEKKAPKVIDVSYNPQNDFLSVKLENDTLSAVAKAIIDKS
GKNLIVAADAKNLRVSSYILNRPFDQVIEMMSKSNDLTATKDDNGFFYIEKNKDATVTVNGSTQKVKPPKASSGLPGYYE
VNLNKNGFLSVKAYIADASDVLTEAAEKLHVNYFLYNKPENEKTTLSADNITFDELLFHIFKGKKYTYKKQDNLYLIGEE
GSEGLRATEVIQLENRSIELVLPSLPKVFSEKLEIKEFVELNGLVASGSKSILEELKLYIKQIDKIVPMVQIEVIIAQYN
KSHDVQTGMKAGLDKINKQVTGGTVFPTTDMTVNSTSVNSLIDAFNGFGIFKLGKVTEAFYLNLKLLENNSIVKVESTPK
IATISGHEAKLSIGETSYYFEQSNRLINSNIGNDILNSGTWKSTDANLSLSIKPYVSIDENVTLTIVVEKSSFLGRAGET
APPGKATQKFESLVRVKNGEMILLGGLDELKKEDSGSGTPLLSRIPIIKWFFSSRKKGKNNSKLHIFIKPTVVY

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=291, Percent_Identity=26.8041237113402, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 70671; Mature: 70671

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKLGYVFIILFFTNSIIAQQDINQLSKQFDALSVQKKGLNETIKIDVSGLTLRDFISS
CCCCHHHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
IAEEHQLNIDVDSELTQQVSNNFFDVTVKDVLIHLVQKYDLEVTFMNNIIVFKKRKAVTV
HHHHHEEEECCCHHHHHHHHCCEEEEEHHHHHHHHHHHCCEEEEEECCEEEEECCCEEEE
VEKKAPKVIDVSYNPQNDFLSVKLENDTLSAVAKAIIDKSGKNLIVAADAKNLRVSSYIL
EECCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHH
NRPFDQVIEMMSKSNDLTATKDDNGFFYIEKNKDATVTVNGSTQKVKPPKASSGLPGYYE
CCCHHHHHHHHHCCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEEE
VNLNKNGFLSVKAYIADASDVLTEAAEKLHVNYFLYNKPENEKTTLSADNITFDELLFHI
EEECCCCEEEEEEEECCHHHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCCHHHHHHHH
FKGKKYTYKKQDNLYLIGEEGSEGLRATEVIQLENRSIELVLPSLPKVFSEKLEIKEFVE
HCCCCEEEECCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHH
LNGLVASGSKSILEELKLYIKQIDKIVPMVQIEVIIAQYNKSHDVQTGMKAGLDKINKQV
HCCEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
TGGTVFPTTDMTVNSTSVNSLIDAFNGFGIFKLGKVTEAFYLNLKLLENNSIVKVESTPK
CCCEEECCCCCEECCCHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEECCCEEEEECCCC
IATISGHEAKLSIGETSYYFEQSNRLINSNIGNDILNSGTWKSTDANLSLSIKPYVSIDE
EEEECCCCEEEEECCCEEEEECCCCEECCCCCCHHHCCCCCCCCCCCEEEEEEEEEEECC
NVTLTIVVEKSSFLGRAGETAPPGKATQKFESLVRVKNGEMILLGGLDELKKEDSGSGTP
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHEECCCCEEEEECCHHHHCCCCCCCCC
LLSRIPIIKWFFSSRKKGKNNSKLHIFIKPTVVY
HHHHCCHHHHHHHHHCCCCCCCEEEEEEEEEEEC
>Mature Secondary Structure
MFKKLGYVFIILFFTNSIIAQQDINQLSKQFDALSVQKKGLNETIKIDVSGLTLRDFISS
CCCCHHHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHH
IAEEHQLNIDVDSELTQQVSNNFFDVTVKDVLIHLVQKYDLEVTFMNNIIVFKKRKAVTV
HHHHHEEEECCCHHHHHHHHCCEEEEEHHHHHHHHHHHCCEEEEEECCEEEEECCCEEEE
VEKKAPKVIDVSYNPQNDFLSVKLENDTLSAVAKAIIDKSGKNLIVAADAKNLRVSSYIL
EECCCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHH
NRPFDQVIEMMSKSNDLTATKDDNGFFYIEKNKDATVTVNGSTQKVKPPKASSGLPGYYE
CCCHHHHHHHHHCCCCCEEEECCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEEE
VNLNKNGFLSVKAYIADASDVLTEAAEKLHVNYFLYNKPENEKTTLSADNITFDELLFHI
EEECCCCEEEEEEEECCHHHHHHHHHHHEEEEEEEEECCCCCCEEEEECCCCHHHHHHHH
FKGKKYTYKKQDNLYLIGEEGSEGLRATEVIQLENRSIELVLPSLPKVFSEKLEIKEFVE
HCCCCEEEECCCCEEEEECCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHH
LNGLVASGSKSILEELKLYIKQIDKIVPMVQIEVIIAQYNKSHDVQTGMKAGLDKINKQV
HCCEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCC
TGGTVFPTTDMTVNSTSVNSLIDAFNGFGIFKLGKVTEAFYLNLKLLENNSIVKVESTPK
CCCEEECCCCCEECCCHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEECCCEEEEECCCC
IATISGHEAKLSIGETSYYFEQSNRLINSNIGNDILNSGTWKSTDANLSLSIKPYVSIDE
EEEECCCCEEEEECCCEEEEECCCCEECCCCCCHHHCCCCCCCCCCCEEEEEEEEEEECC
NVTLTIVVEKSSFLGRAGETAPPGKATQKFESLVRVKNGEMILLGGLDELKKEDSGSGTP
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHEECCCCEEEEECCHHHHCCCCCCCCC
LLSRIPIIKWFFSSRKKGKNNSKLHIFIKPTVVY
HHHHCCHHHHHHHHHCCCCCCCEEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]