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The map label for this gene is pulE [H]

Identifier: 146298380

GI number: 146298380

Start: 678400

End: 679803

Strand: Direct

Name: pulE [H]

Synonym: Fjoh_0617

Alternate gene names: 146298380

Gene position: 678400-679803 (Clockwise)

Preceding gene: 146298379

Following gene: 146298381

Centisome position: 11.13

GC content: 33.48

Gene sequence:

>1404_bases
ATGCAGGATATTATTGTTCTTTCCGAAAACCAGCATATTGTATCAAGTGATCTTGCTAATCAATATCGAATACTTCCTAA
ATCTGTTTCAGAAAATATTCTCGAATTGTATGTTGACAGTGCTAATAGCAGTGCCGACGCAAAAGAAGAACTCGAACTTT
TTTTAGGTAAAAACATTGTTTTCAGCCCAATAGATTCGTATGAAATCGAAAAGGCACTTTCTATTTATTACCGAAAAGAA
AGAATAACAACATCCAGCAAATCTCTAAATTTTGACAGAGGAGATTTTCTTGAAAATTTATTAGAAGAAGCAAAATCACT
CAAATGCAGTGATATTCATTTTGAAGTTTACGAAAAAAACGCCAGAATCAGATTTAGAATTGACGGCCAGTTAATTGAAC
GTTACAAAGTAGAACGCGAAAACTACCTTGAGCTTGTAAATAAAATCAAGATAAAATCTAAATTAAATATTACCGAAAAA
CGTTTACCTCAGGATGGTCGAATTACAAACGACTCTTTTGACATCAGGGTTTCTATTTTACCTACTTTGTTTGGAGAAAA
AATTGTAATGCGTCTTTTGGGTCAGGATGCTTCAAATATTGATTTAAGCACACTAGGTCTACAAAAGGAAGAATTAGAGC
ATTATCTTGAAGCCGTAAAAAAACCAAACGGAATTATTTTAATAAGCGGACCAACCGGTTCCGGTAAAACAACAACGCTC
TACGCAACGTTAAGATTGCTTAATGATAGCCGTAGAAATATAGTTACAGTAGAAGATCCTATTGAGTATACTTTAAAAGG
TATAAATCAGGTACAGCTTAAGGAAGATATCGGCCTGACTTTTTCATCGGCATTAAAATCATTTTTAAGACAAGATCCAG
ATGTTATCATGCTGGGAGAAATTCGTGATTCTGAAACAGCTTTAATGGCCATACGAGCATCGCTTACCGGGCATTTAGTA
TTATCTACAATCCATACCAATTCAGCAATAGGAACCATTTCCAGATTAATTGATATGGGAGTTCCCTCCTATTTAATTGC
CGAAACTTTAAATCTTTCGGTAGCACAGCGATTAATTCGAAAACTCTGTAATCATTGTAAAACCGAAGTTCCTTGTACGA
AAAAAGATTTTCCAATGAACTTTCAATTTCCGTATGAAATATCATCTTACTATAAACCTGTTGGATGCAACCAGTGTTTT
CATACCGGATATAAAGGAAGAACTGCTATTTATGAAGTCTTAACCATTGACACCAAAATAGCCGAAGCTGTAAAAAACAA
TACCATTACAGAATTATTCAGTAACGACGAAAACTATAAATCATTATCAGAAAAAGCTTTTGATATCCTTGCAGAAGGAG
AAACAGCTTTAGAAGAAATATATTCTATTTTAATTAACTTATAA

Upstream 100 bases:

>100_bases
AAATACAGTGCCACTGGCAGGCTTTTCGTCTTTGTTTTTATTTATTATTCTGGTAAAAGACTATTTGCACTTTTATCAAA
AATTAACCGTGATATAAATT

Downstream 100 bases:

>100_bases
CTAAATGTTTAAAAAATTAGGTTACGTATTTATCATACTGTTTTTTACAAATAGTATTATTGCTCAGCAAGACATAAATC
AATTAAGCAAACAATTTGAT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pullulanase secretion protein pulE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MQDIIVLSENQHIVSSDLANQYRILPKSVSENILELYVDSANSSADAKEELELFLGKNIVFSPIDSYEIEKALSIYYRKE
RITTSSKSLNFDRGDFLENLLEEAKSLKCSDIHFEVYEKNARIRFRIDGQLIERYKVERENYLELVNKIKIKSKLNITEK
RLPQDGRITNDSFDIRVSILPTLFGEKIVMRLLGQDASNIDLSTLGLQKEELEHYLEAVKKPNGIILISGPTGSGKTTTL
YATLRLLNDSRRNIVTVEDPIEYTLKGINQVQLKEDIGLTFSSALKSFLRQDPDVIMLGEIRDSETALMAIRASLTGHLV
LSTIHTNSAIGTISRLIDMGVPSYLIAETLNLSVAQRLIRKLCNHCKTEVPCTKKDFPMNFQFPYEISSYYKPVGCNQCF
HTGYKGRTAIYEVLTIDTKIAEAVKNNTITELFSNDENYKSLSEKAFDILAEGETALEEIYSILINL

Sequences:

>Translated_467_residues
MQDIIVLSENQHIVSSDLANQYRILPKSVSENILELYVDSANSSADAKEELELFLGKNIVFSPIDSYEIEKALSIYYRKE
RITTSSKSLNFDRGDFLENLLEEAKSLKCSDIHFEVYEKNARIRFRIDGQLIERYKVERENYLELVNKIKIKSKLNITEK
RLPQDGRITNDSFDIRVSILPTLFGEKIVMRLLGQDASNIDLSTLGLQKEELEHYLEAVKKPNGIILISGPTGSGKTTTL
YATLRLLNDSRRNIVTVEDPIEYTLKGINQVQLKEDIGLTFSSALKSFLRQDPDVIMLGEIRDSETALMAIRASLTGHLV
LSTIHTNSAIGTISRLIDMGVPSYLIAETLNLSVAQRLIRKLCNHCKTEVPCTKKDFPMNFQFPYEISSYYKPVGCNQCF
HTGYKGRTAIYEVLTIDTKIAEAVKNNTITELFSNDENYKSLSEKAFDILAEGETALEEIYSILINL
>Mature_467_residues
MQDIIVLSENQHIVSSDLANQYRILPKSVSENILELYVDSANSSADAKEELELFLGKNIVFSPIDSYEIEKALSIYYRKE
RITTSSKSLNFDRGDFLENLLEEAKSLKCSDIHFEVYEKNARIRFRIDGQLIERYKVERENYLELVNKIKIKSKLNITEK
RLPQDGRITNDSFDIRVSILPTLFGEKIVMRLLGQDASNIDLSTLGLQKEELEHYLEAVKKPNGIILISGPTGSGKTTTL
YATLRLLNDSRRNIVTVEDPIEYTLKGINQVQLKEDIGLTFSSALKSFLRQDPDVIMLGEIRDSETALMAIRASLTGHLV
LSTIHTNSAIGTISRLIDMGVPSYLIAETLNLSVAQRLIRKLCNHCKTEVPCTKKDFPMNFQFPYEISSYYKPVGCNQCF
HTGYKGRTAIYEVLTIDTKIAEAVKNNTITELFSNDENYKSLSEKAFDILAEGETALEEIYSILINL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=431, Percent_Identity=39.2111368909513, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=41.0994764397906, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082188, Length=126, Percent_Identity=49.2063492063492, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52926; Mature: 52926

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIIVLSENQHIVSSDLANQYRILPKSVSENILELYVDSANSSADAKEELELFLGKNIV
CCCEEEECCCCEEHHHHHHHHHEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEE
FSPIDSYEIEKALSIYYRKERITTSSKSLNFDRGDFLENLLEEAKSLKCSDIHFEVYEKN
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC
ARIRFRIDGQLIERYKVERENYLELVNKIKIKSKLNITEKRLPQDGRITNDSFDIRVSIL
CEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEH
PTLFGEKIVMRLLGQDASNIDLSTLGLQKEELEHYLEAVKKPNGIILISGPTGSGKTTTL
HHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEH
YATLRLLNDSRRNIVTVEDPIEYTLKGINQVQLKEDIGLTFSSALKSFLRQDPDVIMLGE
EEHHHHHCCCCCCEEEECCCHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHCCCCEEEEEC
IRDSETALMAIRASLTGHLVLSTIHTNSAIGTISRLIDMGVPSYLIAETLNLSVAQRLIR
CCCCCHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KLCNHCKTEVPCTKKDFPMNFQFPYEISSYYKPVGCNQCFHTGYKGRTAIYEVLTIDTKI
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCHHEEHHHHHHHHH
AEAVKNNTITELFSNDENYKSLSEKAFDILAEGETALEEIYSILINL
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDIIVLSENQHIVSSDLANQYRILPKSVSENILELYVDSANSSADAKEELELFLGKNIV
CCCEEEECCCCEEHHHHHHHHHEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEE
FSPIDSYEIEKALSIYYRKERITTSSKSLNFDRGDFLENLLEEAKSLKCSDIHFEVYEKN
ECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECC
ARIRFRIDGQLIERYKVERENYLELVNKIKIKSKLNITEKRLPQDGRITNDSFDIRVSIL
CEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCEEEEEEH
PTLFGEKIVMRLLGQDASNIDLSTLGLQKEELEHYLEAVKKPNGIILISGPTGSGKTTTL
HHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEH
YATLRLLNDSRRNIVTVEDPIEYTLKGINQVQLKEDIGLTFSSALKSFLRQDPDVIMLGE
EEHHHHHCCCCCCEEEECCCHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHCCCCEEEEEC
IRDSETALMAIRASLTGHLVLSTIHTNSAIGTISRLIDMGVPSYLIAETLNLSVAQRLIR
CCCCCHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KLCNHCKTEVPCTKKDFPMNFQFPYEISSYYKPVGCNQCFHTGYKGRTAIYEVLTIDTKI
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCHHEEHHHHHHHHH
AEAVKNNTITELFSNDENYKSLSEKAFDILAEGETALEEIYSILINL
HHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1738317; 2677007 [H]