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The map label for this gene is nasF [H]

Identifier: 146284404

GI number: 146284404

Start: 4446150

End: 4447481

Strand: Direct

Name: nasF [H]

Synonym: PST_4092

Alternate gene names: 146284404

Gene position: 4446150-4447481 (Clockwise)

Preceding gene: 146284403

Following gene: 146284405

Centisome position: 97.34

GC content: 65.24

Gene sequence:

>1332_bases
ATGACCGACAAGCCGAACAATGACCAGCCCAGCGACCTGACCCTGGCCTCGCGCCGCAGCTTCCTCAAGCATTCGATCAT
CGCCGCCAGCGGGGTCGCCCTGTTCGGCGGCATGTCCCTGGGCATGCGTTCGGCCGCCTGGGCTGCGGGCGGCGACGTGG
AAACCAGCAAGGCCAAGCTCGGCTTCATCGCCCTGACCGACGCCGCACCGCTGTTCGTCGCCGCGGAGAAAGGCTTCTTC
GCCAAGTACGGGATGACCGAGGTCGAGGTGCTCAAGCAGTCTTCCTGGGGCACCACCCGCGACAACCTGGTGCTCGGCTC
GGCGAAGAATGGCATCGATGGCGCGCACATCCTCACGCCCATGCCCTACCTGATCAGTGCCGGCATCGTCACGCCGAACA
ACCAGCCGGTGCCCATGTCGATCCTCGCGCGGCTCAACCTGGCCGGGCAGTGCATCTCGGTGGGCGAGGAGTATCGCGAT
CTGAAGATCGGCCTCGACAGCACGCCGTTCAAGGCGGCGTTGGAGGCGAAGAAGGCCGGCGGCAAGAAGGTCAGCGCCGC
GGTGACCTTTCCCGGCGGCACCCACGACCTGTGGATGCGCTACTGGCTGGCCGCCGGCGGCATCGACCCGAACGCCGACA
TCAACACCATCGTCGTGCCGCCAGCGCAGATGGTCGCCAACATGAAGGTCGGCAGCATGGACACCTTCTGCGTCTGCGAA
CCGTGGAACGCCCAGCTGATCAACCAGAAGATCGGCTACACCGCCAACACCACCGGCGAGCTGTGGCACAACCACCCGGA
AAAGGCCTTCGCCCTGCGCAGCGACTACGTGGCCGCCAACCCCAACGCCGCCCGCGCGCTGACCATGGCGATCATGGAAG
CCCAACAGTTCTGCGAGAAGGCCGAGAACAAGGAAGAAGTGGCGAAGATCTGCTCGCAACGCAAGTGGATCGGCGCGCCC
TACAAGGACATCGTCGACCGCATGAAGGGCAACTTCGCCTACGGCACCGGCAAGGTCATCGAGAACCATCCCGAGCAGAT
GCGCTACTGGGACGACCACGCCTCCTATCCGTTCCAGAGCCACGACCTCTGGTTCCTCACCGAGAACAAGCGCTGGGGCT
ACCTGCCGGCCGACTTCGACAGCCAGGCGCTGATCGCCAAGGTCAACCGCGAAGACATCTGGCGCGCCGCAGCCGGCGAG
CTGAACCTGCCCGCCGAGCTGATCCCGACCTCCACCTCCCGCGGCGTGGAGAAGTTCTTCGACGGCAAGCTGTTCGACCC
CGAGAACCCGCAAGCCTACCTGGACAGCCTGACCATCAAGGCCATGGCCTGA

Upstream 100 bases:

>100_bases
TCGGCTTCCACAACTGACTTTCAGGACAACGGTGTCCACGCTCTCCGGCTCCAGCCGGGCGGTGTGGCGCCGTTTTTTTT
CGCCCAAAGGAAATTCGACC

Downstream 100 bases:

>100_bases
TAACCGCAACCCGCGAGGAAAAGCCCATGAATGCCAACGTCAAACTGCAGCCCGCCAGCACCGAGCCGGTGTCCATTGTC
CGCCCATCCAGAGCCGCTGC

Product: nitrate ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTDKPNNDQPSDLTLASRRSFLKHSIIAASGVALFGGMSLGMRSAAWAAGGDVETSKAKLGFIALTDAAPLFVAAEKGFF
AKYGMTEVEVLKQSSWGTTRDNLVLGSAKNGIDGAHILTPMPYLISAGIVTPNNQPVPMSILARLNLAGQCISVGEEYRD
LKIGLDSTPFKAALEAKKAGGKKVSAAVTFPGGTHDLWMRYWLAAGGIDPNADINTIVVPPAQMVANMKVGSMDTFCVCE
PWNAQLINQKIGYTANTTGELWHNHPEKAFALRSDYVAANPNAARALTMAIMEAQQFCEKAENKEEVAKICSQRKWIGAP
YKDIVDRMKGNFAYGTGKVIENHPEQMRYWDDHASYPFQSHDLWFLTENKRWGYLPADFDSQALIAKVNREDIWRAAAGE
LNLPAELIPTSTSRGVEKFFDGKLFDPENPQAYLDSLTIKAMA

Sequences:

>Translated_443_residues
MTDKPNNDQPSDLTLASRRSFLKHSIIAASGVALFGGMSLGMRSAAWAAGGDVETSKAKLGFIALTDAAPLFVAAEKGFF
AKYGMTEVEVLKQSSWGTTRDNLVLGSAKNGIDGAHILTPMPYLISAGIVTPNNQPVPMSILARLNLAGQCISVGEEYRD
LKIGLDSTPFKAALEAKKAGGKKVSAAVTFPGGTHDLWMRYWLAAGGIDPNADINTIVVPPAQMVANMKVGSMDTFCVCE
PWNAQLINQKIGYTANTTGELWHNHPEKAFALRSDYVAANPNAARALTMAIMEAQQFCEKAENKEEVAKICSQRKWIGAP
YKDIVDRMKGNFAYGTGKVIENHPEQMRYWDDHASYPFQSHDLWFLTENKRWGYLPADFDSQALIAKVNREDIWRAAAGE
LNLPAELIPTSTSRGVEKFFDGKLFDPENPQAYLDSLTIKAMA
>Mature_442_residues
TDKPNNDQPSDLTLASRRSFLKHSIIAASGVALFGGMSLGMRSAAWAAGGDVETSKAKLGFIALTDAAPLFVAAEKGFFA
KYGMTEVEVLKQSSWGTTRDNLVLGSAKNGIDGAHILTPMPYLISAGIVTPNNQPVPMSILARLNLAGQCISVGEEYRDL
KIGLDSTPFKAALEAKKAGGKKVSAAVTFPGGTHDLWMRYWLAAGGIDPNADINTIVVPPAQMVANMKVGSMDTFCVCEP
WNAQLINQKIGYTANTTGELWHNHPEKAFALRSDYVAANPNAARALTMAIMEAQQFCEKAENKEEVAKICSQRKWIGAPY
KDIVDRMKGNFAYGTGKVIENHPEQMRYWDDHASYPFQSHDLWFLTENKRWGYLPADFDSQALIAKVNREDIWRAAAGEL
NLPAELIPTSTSRGVEKFFDGKLFDPENPQAYLDSLTIKAMA

Specific function: Essential component of the nitrate-transporting system. May be the substrate-binding protein [H]

COG id: COG0715

COG function: function code P; ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CmpA/NrtA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 48418; Mature: 48286

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKPNNDQPSDLTLASRRSFLKHSIIAASGVALFGGMSLGMRSAAWAAGGDVETSKAKL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHCCCHHCCCCCCCCCCCCCEE
GFIALTDAAPLFVAAEKGFFAKYGMTEVEVLKQSSWGTTRDNLVLGSAKNGIDGAHILTP
EEEEECCCCCEEEEECCCCEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHCC
MPYLISAGIVTPNNQPVPMSILARLNLAGQCISVGEEYRDLKIGLDSTPFKAALEAKKAG
CHHHHCCCEECCCCCCCHHHHHHHHHHCHHHHHHCHHHCEEEECCCCCCHHHHHHHHHCC
GKKVSAAVTFPGGTHDLWMRYWLAAGGIDPNADINTIVVPPAQMVANMKVGSMDTFCVCE
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHCCCCCCCCCEEEEC
PWNAQLINQKIGYTANTTGELWHNHPEKAFALRSDYVAANPNAARALTMAIMEAQQFCEK
CCCHHHHHHHCCCCCCCCHHHHCCCCHHHHEECCCCEECCCCHHHHHHHHHHHHHHHHHH
AENKEEVAKICSQRKWIGAPYKDIVDRMKGNFAYGTGKVIENHPEQMRYWDDHASYPFQS
CCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEECCCCHHHCCHHHHHCCCCCCCCCCCC
HDLWFLTENKRWGYLPADFDSQALIAKVNREDIWRAAAGELNLPAELIPTSTSRGVEKFF
CCEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHH
DGKLFDPENPQAYLDSLTIKAMA
CCCCCCCCCCHHHHHCEEEEEEC
>Mature Secondary Structure 
TDKPNNDQPSDLTLASRRSFLKHSIIAASGVALFGGMSLGMRSAAWAAGGDVETSKAKL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCHHHCCCHHCCCCCCCCCCCCCEE
GFIALTDAAPLFVAAEKGFFAKYGMTEVEVLKQSSWGTTRDNLVLGSAKNGIDGAHILTP
EEEEECCCCCEEEEECCCCEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHCC
MPYLISAGIVTPNNQPVPMSILARLNLAGQCISVGEEYRDLKIGLDSTPFKAALEAKKAG
CHHHHCCCEECCCCCCCHHHHHHHHHHCHHHHHHCHHHCEEEECCCCCCHHHHHHHHHCC
GKKVSAAVTFPGGTHDLWMRYWLAAGGIDPNADINTIVVPPAQMVANMKVGSMDTFCVCE
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHCCCCCCCCCEEEEC
PWNAQLINQKIGYTANTTGELWHNHPEKAFALRSDYVAANPNAARALTMAIMEAQQFCEK
CCCHHHHHHHCCCCCCCCHHHHCCCCHHHHEECCCCEECCCCHHHHHHHHHHHHHHHHHH
AENKEEVAKICSQRKWIGAPYKDIVDRMKGNFAYGTGKVIENHPEQMRYWDDHASYPFQS
CCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEECCCCHHHCCHHHHHCCCCCCCCCCCC
HDLWFLTENKRWGYLPADFDSQALIAKVNREDIWRAAAGELNLPAELIPTSTSRGVEKFF
CCEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHHCCCCCHHHHHHHH
DGKLFDPENPQAYLDSLTIKAMA
CCCCCCCCCCHHHHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]