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The map label for this gene is rhtB [H]

Identifier: 146284236

GI number: 146284236

Start: 4254452

End: 4255054

Strand: Direct

Name: rhtB [H]

Synonym: PST_3924

Alternate gene names: 146284236

Gene position: 4254452-4255054 (Clockwise)

Preceding gene: 146284235

Following gene: 146284237

Centisome position: 93.15

GC content: 66.5

Gene sequence:

>603_bases
ATGACCGCCACCTACCTCGCCTTTGCCGCCGCCGTAGCCCTGCTGATCGCCTCGCCCGGCCCCGTCGTCGCCCTGGTGGT
GGCCGACGCCCGCCGCCGCTGGCCGCTGTGGACGATTCTCGGTGGCGTGGTATCGGCCCAGGTACTGATGATCGCCGCGC
TGGTGATGATCTACCTGGCGCTGGACCTGCAACCGATCGTGCTCGAGGCCGGCCAGATCCTTGGCGGGCTCTACCTTATC
TGGCTCGGCTGCGACGGCCTGTGCGGCGGCCGCAGCGACACCGCCACGCCAACCCGCAGTGAAGCGCACTATTTCTGGCG
GGCCATGGCGGTGGGCCTGTCCAACCCGAAGGACATCCTGTTCTTCCTCGCCTTTCTGCCCGGCTTCATCCTGCCCGCGC
AGCCGTTCGCGCCGCAGGCGACAGTGCTCATCCTGATCTGGGCAATCATCGACCTGAGCATCCTGCTCACCTATAGCCTG
CTGTCGCGTCGCCTGGCAGGCGAAGGGCTGATCCAGCGCCTGCTGGATATCCTGCCCAACTACTTCCTCATCGGCCTGGG
TCTGGTTTCCTGCACCCTCGGACTGTCCCGCCTGCTGCATTGA

Upstream 100 bases:

>100_bases
CCCGCTTCGACGGCAGCGACCGCTGGATACAGCGGGCATTCGCCATTTCCAATCCCAACTTCTACACCGAGCGCCTGGGC
TTCGGCCTGGTGACCGAACT

Downstream 100 bases:

>100_bases
CCCTGCGCCAGCAAGGGCAATATCAAAACTTTTTGATATTGCCCTTGCTGGGTGAAAACGGTCCGACTAGACTTCGCGGC
CTATGAGCCTGCGCTTTCCC

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MTATYLAFAAAVALLIASPGPVVALVVADARRRWPLWTILGGVVSAQVLMIAALVMIYLALDLQPIVLEAGQILGGLYLI
WLGCDGLCGGRSDTATPTRSEAHYFWRAMAVGLSNPKDILFFLAFLPGFILPAQPFAPQATVLILIWAIIDLSILLTYSL
LSRRLAGEGLIQRLLDILPNYFLIGLGLVSCTLGLSRLLH

Sequences:

>Translated_200_residues
MTATYLAFAAAVALLIASPGPVVALVVADARRRWPLWTILGGVVSAQVLMIAALVMIYLALDLQPIVLEAGQILGGLYLI
WLGCDGLCGGRSDTATPTRSEAHYFWRAMAVGLSNPKDILFFLAFLPGFILPAQPFAPQATVLILIWAIIDLSILLTYSL
LSRRLAGEGLIQRLLDILPNYFLIGLGLVSCTLGLSRLLH
>Mature_199_residues
TATYLAFAAAVALLIASPGPVVALVVADARRRWPLWTILGGVVSAQVLMIAALVMIYLALDLQPIVLEAGQILGGLYLIW
LGCDGLCGGRSDTATPTRSEAHYFWRAMAVGLSNPKDILFFLAFLPGFILPAQPFAPQATVLILIWAIIDLSILLTYSLL
SRRLAGEGLIQRLLDILPNYFLIGLGLVSCTLGLSRLLH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=104, Percent_Identity=37.5, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21542; Mature: 21411

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATYLAFAAAVALLIASPGPVVALVVADARRRWPLWTILGGVVSAQVLMIAALVMIYLA
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLQPIVLEAGQILGGLYLIWLGCDGLCGGRSDTATPTRSEAHYFWRAMAVGLSNPKDIL
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
FFLAFLPGFILPAQPFAPQATVLILIWAIIDLSILLTYSLLSRRLAGEGLIQRLLDILPN
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFLIGLGLVSCTLGLSRLLH
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TATYLAFAAAVALLIASPGPVVALVVADARRRWPLWTILGGVVSAQVLMIAALVMIYLA
CHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLQPIVLEAGQILGGLYLIWLGCDGLCGGRSDTATPTRSEAHYFWRAMAVGLSNPKDIL
HCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
FFLAFLPGFILPAQPFAPQATVLILIWAIIDLSILLTYSLLSRRLAGEGLIQRLLDILPN
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFLIGLGLVSCTLGLSRLLH
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]