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The map label for this gene is tolC [H]

Identifier: 146284129

GI number: 146284129

Start: 4128925

End: 4130412

Strand: Direct

Name: tolC [H]

Synonym: PST_3817

Alternate gene names: 146284129

Gene position: 4128925-4130412 (Clockwise)

Preceding gene: 146284127

Following gene: 146284131

Centisome position: 90.4

GC content: 64.18

Gene sequence:

>1488_bases
TTGCCCATCAGTTCTATTCAGGATGGACCCAACATGCTGCGCAGACTCCCCCTGGCTCTCGCCGTGGCCACCCTCACTGC
CGGAGGGGCCTGGGCGCAAGAGACCGTCCCCCTCGCCAGCAAGACCGATCTGGTCAGCGTCTACCAGCAAGCCGTCAACA
ACAACGCCGACCTGGCTGCAGCTCGCGCACAGTTCCGCGCCCGCCAGGAAGTGGTGCCACAGGCACGCGCCGGCCTGCTG
CCCAACCTCAGCGCCGGCGCCGAGCTGAGCGACACCGAAACGGACGTCGATACCAGCACGGGCTCCACGTCGCTATCACG
CAGTGGCACCGCCTATCAGGCGACGCTGAGCCAGCCGATCTTCCGTGCTGATCGCTGGTTCCAGCTCAAGGCCGCGGAAG
CCGTGAGCGAGCAGGCCGCAATCGAATACTCCATCGCCGAGCAGGACCTGATCCTGCAGAGCGCCCAGGCCTACTTCGCC
GTGCTGCGCGCGCAGGACAACCTGGCGGCGGTGAAGGCCGAAGAAGCCGCATTCAAGCGCCAGCTCGACCAGGCCAACGA
ACGCTTCGAGGTCGGTCTTTCCGACAAGACCGATGTGCTCGAGGCCCAGGCCGGGTTCGATACGGCGCGGGCCAACCGCT
CCATCGCCCAGCGCCAGGTTGACGATGCCTTCCAGGCTCTGTTCACCCTGACCAACCGCGAGTACATCGCCCTGGAAGGC
ATCCGCCACACCCTGCCGGTGCTGGCCCCGGTACCCAACGAAGCCAAGGCCTGGGTCGATACGGCGACGCGGCAGAACCT
CAACCTGCTGGCAGTGAATTACGCGGTGACAGCGGCGGAAGAAACCCTGCGCCAGCGCCGCTCCGGCCATGCACCGACGC
TCGATGCGGTGGCGTCCTATCGCAAGGGCGACAACGACTCGCTGGGTTTCAGCAATTCCCAGAGTATTCCGGGCTTCGAC
ATGCCGCGCTATACCGGCGATGTCGAACAACGCAGCATTGGCCTGCAGCTGAACATCCCGATCTACAGCGGCGGCCTGAC
CACTTCGCAGAGTCGTGAGGCCTATTACCAGCTCAGCCAGAGCGAACAGCAGCGTGAGAGCCTGCGCCGCCAAGTGGTCG
AATCCACACGCAACCTGCATCGGGCGGTGAATACGGATATCGAACAGGTCGAAGCCCGCCGGCAGTCGATCATCTCCAAC
CAGAGCGCGCTCGAGGCCACTGAAATCGGCTATCAGGTCGGCACGCGTAACATCGTCGACGTGCTCGACACTCAGCGCCG
GCTGTATGGCGCTGTGCGCGACTACAACAACGCCCGCTACGACTACATTCTCGACAACCTGCGTCTGAAACAGGCCGCCG
GCACACTCAACCCGGACGACTTGCAGCAGCTGGCAGCGTATCTGAAGCCAGATTACAACCCGGACACCGACTTCCTGCCG
CCGGACCTGCCGCAGACCATCGGTCAGCCGGTGCGGGTGGATTATTGA

Upstream 100 bases:

>100_bases
GCGAAACTAGTCGACCGTTCGGTCATTTGCCCGGACCGCCATCGCTGCCTCGCACCACGGCGCACGCGCGCCTTGACCCC
CTGACAGCTGCCGCTTAGCC

Downstream 100 bases:

>100_bases
GCCAGGGGGCTCGCTTGAACCTTGACCCGGTGGGCTAAAGCCCACCAGGCGCTCGCACCCCAGACGGCGCAATGACGCCG
ACAGTTAATCCGACTTGCTC

Product: outer membrane efflux protein TolC, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MPISSIQDGPNMLRRLPLALAVATLTAGGAWAQETVPLASKTDLVSVYQQAVNNNADLAAARAQFRARQEVVPQARAGLL
PNLSAGAELSDTETDVDTSTGSTSLSRSGTAYQATLSQPIFRADRWFQLKAAEAVSEQAAIEYSIAEQDLILQSAQAYFA
VLRAQDNLAAVKAEEAAFKRQLDQANERFEVGLSDKTDVLEAQAGFDTARANRSIAQRQVDDAFQALFTLTNREYIALEG
IRHTLPVLAPVPNEAKAWVDTATRQNLNLLAVNYAVTAAEETLRQRRSGHAPTLDAVASYRKGDNDSLGFSNSQSIPGFD
MPRYTGDVEQRSIGLQLNIPIYSGGLTTSQSREAYYQLSQSEQQRESLRRQVVESTRNLHRAVNTDIEQVEARRQSIISN
QSALEATEIGYQVGTRNIVDVLDTQRRLYGAVRDYNNARYDYILDNLRLKQAAGTLNPDDLQQLAAYLKPDYNPDTDFLP
PDLPQTIGQPVRVDY

Sequences:

>Translated_495_residues
MPISSIQDGPNMLRRLPLALAVATLTAGGAWAQETVPLASKTDLVSVYQQAVNNNADLAAARAQFRARQEVVPQARAGLL
PNLSAGAELSDTETDVDTSTGSTSLSRSGTAYQATLSQPIFRADRWFQLKAAEAVSEQAAIEYSIAEQDLILQSAQAYFA
VLRAQDNLAAVKAEEAAFKRQLDQANERFEVGLSDKTDVLEAQAGFDTARANRSIAQRQVDDAFQALFTLTNREYIALEG
IRHTLPVLAPVPNEAKAWVDTATRQNLNLLAVNYAVTAAEETLRQRRSGHAPTLDAVASYRKGDNDSLGFSNSQSIPGFD
MPRYTGDVEQRSIGLQLNIPIYSGGLTTSQSREAYYQLSQSEQQRESLRRQVVESTRNLHRAVNTDIEQVEARRQSIISN
QSALEATEIGYQVGTRNIVDVLDTQRRLYGAVRDYNNARYDYILDNLRLKQAAGTLNPDDLQQLAAYLKPDYNPDTDFLP
PDLPQTIGQPVRVDY
>Mature_494_residues
PISSIQDGPNMLRRLPLALAVATLTAGGAWAQETVPLASKTDLVSVYQQAVNNNADLAAARAQFRARQEVVPQARAGLLP
NLSAGAELSDTETDVDTSTGSTSLSRSGTAYQATLSQPIFRADRWFQLKAAEAVSEQAAIEYSIAEQDLILQSAQAYFAV
LRAQDNLAAVKAEEAAFKRQLDQANERFEVGLSDKTDVLEAQAGFDTARANRSIAQRQVDDAFQALFTLTNREYIALEGI
RHTLPVLAPVPNEAKAWVDTATRQNLNLLAVNYAVTAAEETLRQRRSGHAPTLDAVASYRKGDNDSLGFSNSQSIPGFDM
PRYTGDVEQRSIGLQLNIPIYSGGLTTSQSREAYYQLSQSEQQRESLRRQVVESTRNLHRAVNTDIEQVEARRQSIISNQ
SALEATEIGYQVGTRNIVDVLDTQRRLYGAVRDYNNARYDYILDNLRLKQAAGTLNPDDLQQLAAYLKPDYNPDTDFLPP
DLPQTIGQPVRVDY

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=444, Percent_Identity=35.1351351351351, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54544; Mature: 54413

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISSIQDGPNMLRRLPLALAVATLTAGGAWAQETVPLASKTDLVSVYQQAVNNNADLAA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCHHHH
ARAQFRARQEVVPQARAGLLPNLSAGAELSDTETDVDTSTGSTSLSRSGTAYQATLSQPI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCHH
FRADRWFQLKAAEAVSEQAAIEYSIAEQDLILQSAQAYFAVLRAQDNLAAVKAEEAAFKR
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QLDQANERFEVGLSDKTDVLEAQAGFDTARANRSIAQRQVDDAFQALFTLTNREYIALEG
HHHHCCCCEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
IRHTLPVLAPVPNEAKAWVDTATRQNLNLLAVNYAVTAAEETLRQRRSGHAPTLDAVASY
HHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHH
RKGDNDSLGFSNSQSIPGFDMPRYTGDVEQRSIGLQLNIPIYSGGLTTSQSREAYYQLSQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECEEEECEEEECCCCCCHHHHHHHHHHH
SEQQRESLRRQVVESTRNLHRAVNTDIEQVEARRQSIISNQSALEATEIGYQVGTRNIVD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHH
VLDTQRRLYGAVRDYNNARYDYILDNLRLKQAAGTLNPDDLQQLAAYLKPDYNPDTDFLP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PDLPQTIGQPVRVDY
CCCHHHCCCCEECCC
>Mature Secondary Structure 
PISSIQDGPNMLRRLPLALAVATLTAGGAWAQETVPLASKTDLVSVYQQAVNNNADLAA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCHHHH
ARAQFRARQEVVPQARAGLLPNLSAGAELSDTETDVDTSTGSTSLSRSGTAYQATLSQPI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCHH
FRADRWFQLKAAEAVSEQAAIEYSIAEQDLILQSAQAYFAVLRAQDNLAAVKAEEAAFKR
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
QLDQANERFEVGLSDKTDVLEAQAGFDTARANRSIAQRQVDDAFQALFTLTNREYIALEG
HHHHCCCCEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
IRHTLPVLAPVPNEAKAWVDTATRQNLNLLAVNYAVTAAEETLRQRRSGHAPTLDAVASY
HHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHH
RKGDNDSLGFSNSQSIPGFDMPRYTGDVEQRSIGLQLNIPIYSGGLTTSQSREAYYQLSQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECEEEECEEEECCCCCCHHHHHHHHHHH
SEQQRESLRRQVVESTRNLHRAVNTDIEQVEARRQSIISNQSALEATEIGYQVGTRNIVD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHH
VLDTQRRLYGAVRDYNNARYDYILDNLRLKQAAGTLNPDDLQQLAAYLKPDYNPDTDFLP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PDLPQTIGQPVRVDY
CCCHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]