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The map label for this gene is rhdA [H]

Identifier: 146284111

GI number: 146284111

Start: 4106055

End: 4106870

Strand: Direct

Name: rhdA [H]

Synonym: PST_3799

Alternate gene names: 146284111

Gene position: 4106055-4106870 (Clockwise)

Preceding gene: 146284110

Following gene: 146284112

Centisome position: 89.9

GC content: 68.63

Gene sequence:

>816_bases
GTGTCCGCCTTTTCCAATCTGCCACTGGTGATCGAGCCCGCCGATCTGGCCACCCGCCTCGATGCGCCCGAGCTGATCCT
GGTCGACCTGACCAGTGCTGCCCGCTATGCCGAAGGCCACCTGCCGGGCGCCCGCTTCGTCGATCCCAAACAGACCCAAC
TGGGCCGACCGCCCGCACCAGGCCTGCTGCCCGAACAGGCGCAGCTGGAACGGGTGTTCGGCGCGCTGGGCCATCGCCCC
GATGCGGTGTACGTGGTGTACGACGACGAAGGCGGCGGCTGGGCCGGGCGCTTCATCTGGTTGCTCGACGTGATCGGCCA
CCGGCACTACCACTATCTCAACGGCGGGCTGCATGCCTGGCTGGACGAAGCCCGTCCGCTGAGCCGGGAGATACCCGAAG
CGGCGACCGGTACACCGCACCTGGTCGTGGACGGCGCCCCGACCGCCACTCGCCACTACATCGAGAGCCGCCTCGGCGCC
GACGACCTGGTGATCTGGGACGCCCGCTCGCCGGCCGAATACCGCGGCGAGAAGCTCTTCGCCGCCCGCGGAGGCCATAT
CCCCGGGGCGGTCAATTTCGAGTGGACGGCCGCCATGGACCCCGCCCGCGCGTTGCGCATCCGCGAAGACATCGCCGAGC
GCCTGCTGGCAATGGGCATCGTTCCGGACAAGGAAATCGTCACCCACTGCCAGACTCACCACCGCTCCGGCTTCACCTAC
CTGCTGGCCAAGGCGCTGGGCTATCCCCGCGTCAAGGGCTATGCCGGTTCCTGGGCCGAATGGGGCAACCTGCCTGACAC
CCCGGTGCAGCAATGA

Upstream 100 bases:

>100_bases
CATCACAATGCATCGAGCGCGCTTTGAGTCTGTTCGGCAGGCGCGGGGGCTGAGCTTTTTCGCTAGACTTCGCCCCTTCA
ACGACTAGTCGAGGCCTGCC

Downstream 100 bases:

>100_bases
AAAGGACACACATGAAAGACCGCCTGTTCGTCATCAGCCAGTATGTGCTGCCGCATCACCTGATTTCCCGCCTGGCCGGC
TGCCTGGCCGAATGCCGCCT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSAFSNLPLVIEPADLATRLDAPELILVDLTSAARYAEGHLPGARFVDPKQTQLGRPPAPGLLPEQAQLERVFGALGHRP
DAVYVVYDDEGGGWAGRFIWLLDVIGHRHYHYLNGGLHAWLDEARPLSREIPEAATGTPHLVVDGAPTATRHYIESRLGA
DDLVIWDARSPAEYRGEKLFAARGGHIPGAVNFEWTAAMDPARALRIREDIAERLLAMGIVPDKEIVTHCQTHHRSGFTY
LLAKALGYPRVKGYAGSWAEWGNLPDTPVQQ

Sequences:

>Translated_271_residues
MSAFSNLPLVIEPADLATRLDAPELILVDLTSAARYAEGHLPGARFVDPKQTQLGRPPAPGLLPEQAQLERVFGALGHRP
DAVYVVYDDEGGGWAGRFIWLLDVIGHRHYHYLNGGLHAWLDEARPLSREIPEAATGTPHLVVDGAPTATRHYIESRLGA
DDLVIWDARSPAEYRGEKLFAARGGHIPGAVNFEWTAAMDPARALRIREDIAERLLAMGIVPDKEIVTHCQTHHRSGFTY
LLAKALGYPRVKGYAGSWAEWGNLPDTPVQQ
>Mature_270_residues
SAFSNLPLVIEPADLATRLDAPELILVDLTSAARYAEGHLPGARFVDPKQTQLGRPPAPGLLPEQAQLERVFGALGHRPD
AVYVVYDDEGGGWAGRFIWLLDVIGHRHYHYLNGGLHAWLDEARPLSREIPEAATGTPHLVVDGAPTATRHYIESRLGAD
DLVIWDARSPAEYRGEKLFAARGGHIPGAVNFEWTAAMDPARALRIREDIAERLLAMGIVPDKEIVTHCQTHHRSGFTYL
LAKALGYPRVKGYAGSWAEWGNLPDTPVQQ

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=244, Percent_Identity=27.4590163934426, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI61835204, Length=231, Percent_Identity=29.4372294372294, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI194473681, Length=231, Percent_Identity=29.4372294372294, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI194473668, Length=231, Percent_Identity=29.4372294372294, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI87082121, Length=274, Percent_Identity=29.1970802919708, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081967, Length=249, Percent_Identity=24.8995983935743, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=279, Percent_Identity=22.5806451612903, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 29811; Mature: 29680

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFSNLPLVIEPADLATRLDAPELILVDLTSAARYAEGHLPGARFVDPKQTQLGRPPAP
CCCCCCCCEEEECHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
GLLPEQAQLERVFGALGHRPDAVYVVYDDEGGGWAGRFIWLLDVIGHRHYHYLNGGLHAW
CCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHH
LDEARPLSREIPEAATGTPHLVVDGAPTATRHYIESRLGADDLVIWDARSPAEYRGEKLF
HHHCCCHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHCCCEEE
AARGGHIPGAVNFEWTAAMDPARALRIREDIAERLLAMGIVPDKEIVTHCQTHHRSGFTY
EECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
LLAKALGYPRVKGYAGSWAEWGNLPDTPVQQ
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure 
SAFSNLPLVIEPADLATRLDAPELILVDLTSAARYAEGHLPGARFVDPKQTQLGRPPAP
CCCCCCCEEEECHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHCCCCCCC
GLLPEQAQLERVFGALGHRPDAVYVVYDDEGGGWAGRFIWLLDVIGHRHYHYLNGGLHAW
CCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHH
LDEARPLSREIPEAATGTPHLVVDGAPTATRHYIESRLGADDLVIWDARSPAEYRGEKLF
HHHCCCHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCHHHCCCEEE
AARGGHIPGAVNFEWTAAMDPARALRIREDIAERLLAMGIVPDKEIVTHCQTHHRSGFTY
EECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
LLAKALGYPRVKGYAGSWAEWGNLPDTPVQQ
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]