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The map label for this gene is hemL

Identifier: 146284070

GI number: 146284070

Start: 4066280

End: 4067563

Strand: Direct

Name: hemL

Synonym: PST_3758

Alternate gene names: 146284070

Gene position: 4066280-4067563 (Clockwise)

Preceding gene: 146284069

Following gene: 146284071

Centisome position: 89.03

GC content: 67.29

Gene sequence:

>1284_bases
ATGTCCCGTTCCGAAACCCTCTTTGCCAGCGCCCAGACCCACATCCCCGGCGGCGTCAACTCGCCGGTTCGCGCCTTCCG
CAGCGTCGGCGGCACCCCGCTGTTCCTCAAGCACGCCGAAGGCGCCTATGTCATCGACGAGGACGACAAGCGCTACGTCG
ACTACGTCGGCTCCTGGGGCCCGATGATCCTCGGCCACAGCCACCCGCAGGTACTGGATGCCGTGCGTCGCCAGCTCGAG
CACGGGCTGTCCTACGGCGCACCGACCGCGATGGAAACCGAGATGGCAGAGCTGGTCTGCCGCCTGGTGCCGTCCATGGA
GATGGTCCGCATGGTCAGCTCCGGCACCGAGGCAACCATGAGCGCGATCCGCCTGGCCCGTGGTTACACCGGTCGCGACG
CCATCATCAAGTTCGAGGGCTGCTATCACGGCCACTCCGACAGCCTGCTGGTGAAAGCCGGCTCCGGCGCCCTGACCCAG
GGCGTACCAAGCTCAGCGGGCGTGCCGGCGGACTTCGCCAAGCACACCCTGACCCTGGCCTACAACGACCTCGACGAAGT
CGAAGCCACGCTGAAGGAAAAGGGCGAGCAGGTCGCCTGCATCATCGTCGAGCCGGTGGCCGGCAACATGAACTGCGTGC
CGCCGGCGCCGGGCTTCCTCGAGGGCCTGCGCCGCCTGTGCGACGCGCACGGCGTGGTGCTGATCTTCGACGAGGTGATG
ACCGGTTTCCGCGTCGCCCTCGGCGGTGCCCAGGCCTACTACGGCGTGACGCCGGACCTTTCGACCTTCGGCAAGATCAT
TGGCGGCGGCATGCCGGTGGGCTGCTTCGGCGGCAAGCGCGCCATCATGGAACGCATTGCCCCGCTCGGCCCGGTCTACC
AGGCTGGCACGCTGTCGGGCAATCCACTGGCCATGGCCGCTGGCCTGACCACCCTGGAGTTGATCAGCCGCCCCGGCTTC
CATGACGAACTGGGTGCCTACACCAGCCGCATGCTGCAGGGCCTGCAGGATCGCGCCGATGCCGCCGGCATCCCCTTCGT
CACCACCCAGGTGGGCGGCATGTTCGGTCTGTATTTCAGCGGCGCTGACGACATCGTGACCTTCGCCGACGTCATGGCCA
GCGATGCCGACCGCTTCAAGCGCTTCTTCCACCTGATGCTCGAGGGCGGCGTTTACCTGGCGCCGAGCGCGTTCGAAGCC
GGCTTCACCTCCATCGCCCACGGCGATAAAGAGCTGGCCATCACCCTCGACGCCGCCGAGCGCGCCTTCGCCAAGCTCAA
GTGA

Upstream 100 bases:

>100_bases
TCGAGCGCCGCGCCCAGGCCTTCAGCCAACTGTTCAACACGCCCTGATTCCGCCACGGCGGGTCGCTGATGCCGGCCCGC
CCCTGTTTTGCGAGGCCCGC

Downstream 100 bases:

>100_bases
ACGCGCCGATGGAACTGCTGCAGTCCCTGCTGCTGACGCTTGCCGCTGCTGCCAGCGCGATCCAGATCGGTCGCGCCCGG
CGCCGTTACGACGAAGCGGA

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSRSETLFASAQTHIPGGVNSPVRAFRSVGGTPLFLKHAEGAYVIDEDDKRYVDYVGSWGPMILGHSHPQVLDAVRRQLE
HGLSYGAPTAMETEMAELVCRLVPSMEMVRMVSSGTEATMSAIRLARGYTGRDAIIKFEGCYHGHSDSLLVKAGSGALTQ
GVPSSAGVPADFAKHTLTLAYNDLDEVEATLKEKGEQVACIIVEPVAGNMNCVPPAPGFLEGLRRLCDAHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKRAIMERIAPLGPVYQAGTLSGNPLAMAAGLTTLELISRPGF
HDELGAYTSRMLQGLQDRADAAGIPFVTTQVGGMFGLYFSGADDIVTFADVMASDADRFKRFFHLMLEGGVYLAPSAFEA
GFTSIAHGDKELAITLDAAERAFAKLK

Sequences:

>Translated_427_residues
MSRSETLFASAQTHIPGGVNSPVRAFRSVGGTPLFLKHAEGAYVIDEDDKRYVDYVGSWGPMILGHSHPQVLDAVRRQLE
HGLSYGAPTAMETEMAELVCRLVPSMEMVRMVSSGTEATMSAIRLARGYTGRDAIIKFEGCYHGHSDSLLVKAGSGALTQ
GVPSSAGVPADFAKHTLTLAYNDLDEVEATLKEKGEQVACIIVEPVAGNMNCVPPAPGFLEGLRRLCDAHGVVLIFDEVM
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKRAIMERIAPLGPVYQAGTLSGNPLAMAAGLTTLELISRPGF
HDELGAYTSRMLQGLQDRADAAGIPFVTTQVGGMFGLYFSGADDIVTFADVMASDADRFKRFFHLMLEGGVYLAPSAFEA
GFTSIAHGDKELAITLDAAERAFAKLK
>Mature_426_residues
SRSETLFASAQTHIPGGVNSPVRAFRSVGGTPLFLKHAEGAYVIDEDDKRYVDYVGSWGPMILGHSHPQVLDAVRRQLEH
GLSYGAPTAMETEMAELVCRLVPSMEMVRMVSSGTEATMSAIRLARGYTGRDAIIKFEGCYHGHSDSLLVKAGSGALTQG
VPSSAGVPADFAKHTLTLAYNDLDEVEATLKEKGEQVACIIVEPVAGNMNCVPPAPGFLEGLRRLCDAHGVVLIFDEVMT
GFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKRAIMERIAPLGPVYQAGTLSGNPLAMAAGLTTLELISRPGFH
DELGAYTSRMLQGLQDRADAAGIPFVTTQVGGMFGLYFSGADDIVTFADVMASDADRFKRFFHLMLEGGVYLAPSAFEAG
FTSIAHGDKELAITLDAAERAFAKLK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=312, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI37574042, Length=324, Percent_Identity=27.1604938271605, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI24119277, Length=328, Percent_Identity=27.1341463414634, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI226442709, Length=293, Percent_Identity=27.6450511945392, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI226442705, Length=324, Percent_Identity=26.2345679012346, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI284507298, Length=231, Percent_Identity=31.1688311688312, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=66.1971830985916, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1788044, Length=317, Percent_Identity=32.807570977918, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789016, Length=354, Percent_Identity=31.0734463276836, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789759, Length=306, Percent_Identity=31.0457516339869, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1787560, Length=342, Percent_Identity=30.9941520467836, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693181, Length=292, Percent_Identity=29.4520547945205, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786991, Length=382, Percent_Identity=26.1780104712042, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144271, Length=298, Percent_Identity=30.8724832214765, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=343, Percent_Identity=26.8221574344023, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=35.6643356643357, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17541228, Length=356, Percent_Identity=27.247191011236, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=342, Percent_Identity=28.0701754385965, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=312, Percent_Identity=25, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21357415, Length=297, Percent_Identity=27.9461279461279, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356575, Length=344, Percent_Identity=25.2906976744186, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_PSEU5 (A4VQY0)

Other databases:

- EMBL:   CP000304
- RefSeq:   YP_001174223.1
- ProteinModelPortal:   A4VQY0
- SMR:   A4VQY0
- STRING:   A4VQY0
- GeneID:   5096719
- GenomeReviews:   CP000304_GR
- KEGG:   psa:PST_3758
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- PhylomeDB:   A4VQY0
- ProtClustDB:   PRK00062
- BioCyc:   PSTU379731:PST_3758-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45340; Mature: 45209

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSETLFASAQTHIPGGVNSPVRAFRSVGGTPLFLKHAEGAYVIDEDDKRYVDYVGSWG
CCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHCCCC
PMILGHSHPQVLDAVRRQLEHGLSYGAPTAMETEMAELVCRLVPSMEMVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTGRDAIIKFEGCYHGHSDSLLVKAGSGALTQGVPSSAGVPADFAKHTLTLA
HHHHHHCCCCCCCEEEEEECEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHEEEEE
YNDLDEVEATLKEKGEQVACIIVEPVAGNMNCVPPAPGFLEGLRRLCDAHGVVLIFDEVM
ECCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEHHHHH
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKRAIMERIAPLGPVYQAGTLSG
HHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCC
NPLAMAAGLTTLELISRPGFHDELGAYTSRMLQGLQDRADAAGIPFVTTQVGGMFGLYFS
CCEEHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCEEEEEEC
GADDIVTFADVMASDADRFKRFFHLMLEGGVYLAPSAFEAGFTSIAHGDKELAITLDAAE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHCCHHHHCCCCEEEEEECHHH
RAFAKLK
HHHHHCC
>Mature Secondary Structure 
SRSETLFASAQTHIPGGVNSPVRAFRSVGGTPLFLKHAEGAYVIDEDDKRYVDYVGSWG
CCCCEEEEECCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCHHHHHHCCCC
PMILGHSHPQVLDAVRRQLEHGLSYGAPTAMETEMAELVCRLVPSMEMVRMVSSGTEATM
CEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTGRDAIIKFEGCYHGHSDSLLVKAGSGALTQGVPSSAGVPADFAKHTLTLA
HHHHHHCCCCCCCEEEEEECEECCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHEEEEE
YNDLDEVEATLKEKGEQVACIIVEPVAGNMNCVPPAPGFLEGLRRLCDAHGVVLIFDEVM
ECCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEHHHHH
TGFRVALGGAQAYYGVTPDLSTFGKIIGGGMPVGCFGGKRAIMERIAPLGPVYQAGTLSG
HHHHHHHCCCCEEECCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHCCCCCCCCCCCCCC
NPLAMAAGLTTLELISRPGFHDELGAYTSRMLQGLQDRADAAGIPFVTTQVGGMFGLYFS
CCEEHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCEEEEEEC
GADDIVTFADVMASDADRFKRFFHLMLEGGVYLAPSAFEAGFTSIAHGDKELAITLDAAE
CCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCHHHHCCHHHHCCCCEEEEEECHHH
RAFAKLK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA