Click here to switch to the map view.

The map label for this gene is ilvA [H]

Identifier: 146284056

GI number: 146284056

Start: 4047907

End: 4048647

Strand: Direct

Name: ilvA [H]

Synonym: PST_3744

Alternate gene names: 146284056

Gene position: 4047907-4048647 (Clockwise)

Preceding gene: 146284053

Following gene: 146284057

Centisome position: 88.63

GC content: 67.34

Gene sequence:

>741_bases
ATGATCAGCGCCACCCGCGGCAATCACGGCATCAGCCTGGCGATGGCCGCGCGACAGGCCGGCGTGCCGCTGAAGATTCT
GGTACCGCTGGGCAACTCGACGGAAAAGAATGCGGCGATGCGCGCCTGCGGCGCCGAAGTCATCGAACACGGTGAGGATT
TCGACACGGCGCGCCAGCAAGCCGCCGAGCTGGCGAGACAGAGCGGCTGGCATTTCGTCCCCGCGCTGCATCCGGACCTG
ATTCGCGGGGTCGCCACCTATGCCCTGGAGCTCTTCGAGGCCGCGCCGCCGCTGCGCCGCGTCTACGTGCCCATCGGCAT
GGGCTCGGGGATCTGCGGCCTGATTCGCGTCCGCGACCTGCTGGGGCTGGACTGCGAAATCGTCGGCGTCGTTTCCAGCG
CAGCGGACGCATACGCACAGAGCTTCGAGCAGGGGCGGATCGTCTGCACCAAGACCGCCGATACGTTCGCCGATGGCATG
GCCTGCCGGATGCCTGCTGCCGAAGCACTGGAGATCATTGCCGAAGGTGCCGCACGCATCGTGCGTGTCGATGACGGCGA
AATCCGCCGGGCGATCGTCGCGCTCCACGAAGACACCCACAACACCGCGGAGGGCGCCGGCGCCGCCGCACTGGCTGCCG
CCATTCAGGAACGCGCGCTTAACACGGGGGAACGCATTGCCGTCGTGCTCAGCGGCGCCAACATCGATCGCCGTGTGCTG
GCGGGGATATTGACGAGCTGA

Upstream 100 bases:

>100_bases
GGTCTAGCACGAAAACCACACGCCGGCCGGTGCATTCAAGGTTCGCGGCGGCCTGCTCTACGTGCATGAGCTGCTCGGGC
GCGAGCCACGACTGCCCGGC

Downstream 100 bases:

>100_bases
CGGGTGCGGCGTCGCAGTTCGATCATCTGCAACCCTCGCTGCGACCTGCTTGAAAAGCTTACCTATACGTCTGTACAGCG
GTTTCAGTGAGCAAACCAAC

Product: hypothetical protein

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MISATRGNHGISLAMAARQAGVPLKILVPLGNSTEKNAAMRACGAEVIEHGEDFDTARQQAAELARQSGWHFVPALHPDL
IRGVATYALELFEAAPPLRRVYVPIGMGSGICGLIRVRDLLGLDCEIVGVVSSAADAYAQSFEQGRIVCTKTADTFADGM
ACRMPAAEALEIIAEGAARIVRVDDGEIRRAIVALHEDTHNTAEGAGAAALAAAIQERALNTGERIAVVLSGANIDRRVL
AGILTS

Sequences:

>Translated_246_residues
MISATRGNHGISLAMAARQAGVPLKILVPLGNSTEKNAAMRACGAEVIEHGEDFDTARQQAAELARQSGWHFVPALHPDL
IRGVATYALELFEAAPPLRRVYVPIGMGSGICGLIRVRDLLGLDCEIVGVVSSAADAYAQSFEQGRIVCTKTADTFADGM
ACRMPAAEALEIIAEGAARIVRVDDGEIRRAIVALHEDTHNTAEGAGAAALAAAIQERALNTGERIAVVLSGANIDRRVL
AGILTS
>Mature_246_residues
MISATRGNHGISLAMAARQAGVPLKILVPLGNSTEKNAAMRACGAEVIEHGEDFDTARQQAAELARQSGWHFVPALHPDL
IRGVATYALELFEAAPPLRRVYVPIGMGSGICGLIRVRDLLGLDCEIVGVVSSAADAYAQSFEQGRIVCTKTADTFADGM
ACRMPAAEALEIIAEGAARIVRVDDGEIRRAIVALHEDTHNTAEGAGAAALAAAIQERALNTGERIAVVLSGANIDRRVL
AGILTS

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=242, Percent_Identity=26.8595041322314, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1790207, Length=239, Percent_Identity=33.8912133891213, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789505, Length=245, Percent_Identity=28.5714285714286, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71991565, Length=242, Percent_Identity=28.099173553719, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17537387, Length=237, Percent_Identity=29.535864978903, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322631, Length=246, Percent_Identity=29.6747967479675, Blast_Score=99, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320930, Length=246, Percent_Identity=28.0487804878049, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21355833, Length=246, Percent_Identity=30.0813008130081, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR005787
- InterPro:   IPR001721 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 25803; Mature: 25803

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISATRGNHGISLAMAARQAGVPLKILVPLGNSTEKNAAMRACGAEVIEHGEDFDTARQQ
CCCCCCCCCCEEEEEEHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
AAELARQSGWHFVPALHPDLIRGVATYALELFEAAPPLRRVYVPIGMGSGICGLIRVRDL
HHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
LGLDCEIVGVVSSAADAYAQSFEQGRIVCTKTADTFADGMACRMPAAEALEIIAEGAARI
HCCCCEEEHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCHHHHHHHHHHCCCEE
VRVDDGEIRRAIVALHEDTHNTAEGAGAAALAAAIQERALNTGERIAVVLSGANIDRRVL
EEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
AGILTS
HHHHCC
>Mature Secondary Structure
MISATRGNHGISLAMAARQAGVPLKILVPLGNSTEKNAAMRACGAEVIEHGEDFDTARQQ
CCCCCCCCCCEEEEEEHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
AAELARQSGWHFVPALHPDLIRGVATYALELFEAAPPLRRVYVPIGMGSGICGLIRVRDL
HHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHH
LGLDCEIVGVVSSAADAYAQSFEQGRIVCTKTADTFADGMACRMPAAEALEIIAEGAARI
HCCCCEEEHHHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCCCHHHHHHHHHHCCCEE
VRVDDGEIRRAIVALHEDTHNTAEGAGAAALAAAIQERALNTGERIAVVLSGANIDRRVL
EEECCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHH
AGILTS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3315862; 3550695; 1379743; 9278503; 3003115; 2473940; 9562556 [H]